See discussions, stats, and author profiles for this publication at: https://www.researchgate.

net/publication/326266057

Bark extracts with antioxidant and antimicrobial potential

Poster · June 2018

CITATIONS READS

0 187

6 authors, including:

Duarte Neiva Ângelo Luís

University of Lisbon Universidade da Beira Interior
28 PUBLICATIONS   164 CITATIONS    38 PUBLICATIONS   429 CITATIONS   

SEE PROFILE SEE PROFILE

Jorge Gominho Ana Paula Duarte

University of Lisbon Universidade da Beira Interior
136 PUBLICATIONS   1,652 CITATIONS    101 PUBLICATIONS   1,390 CITATIONS   

SEE PROFILE SEE PROFILE

Some of the authors of this publication are also working on these related projects:

Second-generation biodiesel from Jatropha curcas oil View project

Therapeutic drug monitoring on age related diseases View project

All content following this page was uploaded by Duarte Neiva on 09 July 2018.

The user has requested enhancement of the downloaded file.

 Bark extracts with antioxidant and antimicrobial
potential
Neiva, D.M.[1]*, Luís, Â.[2], Gominho, J.[1], Domingues, F.[2], Duarte, A.P.[2], Pereira, H.[1]
[1] Centro de Estudos Florestais, Instituto Superior de Agronomia, Universidade de Lisboa
[2] Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior

OBJECTIVES
Evaluate n-hexane and ethanol extracts of barks from Acacia dealbata (Ad), Acacia melanoxylon (Am),
Eucalyptus globulus (Eg) and Picea abies (Pa) regarding antioxidant potential and antimicrobial properties.

MATERIAL & METHODS

The antioxidant properties were evaluated regarding the ferric-reducing antioxidant power (FRAP), free radical scavenging activity
(DPPH) and β-carotene bleaching test (BCB, indicative of lipid peroxidation inhibition). For the antimicrobial properties, several strains
of human infecting microorganisms (Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus, Acinetobacter baumannii,
Klebsiella pneumoniae, Pseudomonas aeruginosa, Candida tropicalis) were evaluated using the Disc Diffusion assay.

RESULTS
A
Extraction and antioxidant properties

Table 1. Extraction yield and antioxidant properties of the n-hexane and ethanol extracts of the barks.
TE- Trolox equivalent; AAI – antioxidant activity index (final concentration of DPPH in the control sample/ IC50);
*-Butylated hydroxytoluene (BHT) and **- Rutin standards

n-hexane ethanol
Eg Pa Am Ad Eg Pa Am Ad Std B
Extraction yield (%) 0.5 4.1 1.3 0.8 3.0 9.3 6.8 36.5 -
FRAP - (mgTE/gExt) 15 29 33 26 489 530 323 1295 -
BCB - Inhibition at 1g/L (%) 40 21 17 0 71 76 50 83 100 *
AAI ([DPPH]f/IC50) 0.2 0.0 0.0 0.3 2.7 3.1 2.4 7.2 6 **
A - P. abies n-hexane extract
B - A. dealbata ethanol extract
Disc diffusion assay
Table 2. Diameter of inhibition zones (mm) of n-hexane and ethanol bark extracts and controls
C D
n-hexane ethanol
Control
Eg Pa Am Ad Eg Pa Am Ad
gram positive Tetracycline
E. faecalis - - - - - 14 11 18 25
L. monocytogenes - - - - 18 16 23 29 18
S. aureus 11 8 - 9 17 16 14 21 30
gram negative Tetracycline
A. baumannii 18 9 10 17 11 10 9 15 26 C - visible inhibition of C. tropicalis growth by E.
K. pneumoniae - - - - 16 12 14 21 22 globulus n-hexane extract
P. aeruginosa - - - - 10 10 10 15 12 D - no significant inhibition of C. tropicalis growth
Yeast Amphotericin B by P. abies n-hexane extract
C. tropicalis 25 - 12 24 11 10 9 21 22

Conclusion
• This work shows the presence of compounds with antioxidant and antimicrobial properties in the
crude extracts of the four barks studied
• Ethanol extracts (rich in phenolic compounds) showed high antioxidant activity contrary to the apolar
extracts (low phenolic compounds) with very low antioxidant activity
• A. dealbata bark appears to be the most interesting especially when regarding its ethanol extract,
showing a very high extraction yield and very high antioxidant and antimicrobial activities
• The n-hexane Eg and Ad bark extracts presented a very good fungal growth inhibition.
Acknowledgements
We thank The Navigator Company, Asko Ojaniemi and Catarina Chemetova for providing the raw materials used in this study. The Forest Research
Center (CEF) was financed by Fundação para a Ciência e a Tecnologia (FCT) under UID/AGR/00239/2013. The first author acknowledges a PhD
scholarship (PD/BD/52697/2014) under the SUSFOR doctoral program, and the second author acknowledges a post-doc research fellowship
(BIPD/ICI-FC-BST-UBI 2016) within the scope of the protocol signed between Universidade da Beira Interior and Santander-Totta.

View publication stats