Microbiology Laboratory Training Matrix

DepEd Makati

DAY 1
PERSONS MATERIALS
DATE TIME EVENT OBJECTIVES VENUE SUCCESS INDICATOR
INVOLVED NEEDED
All attendees registered and
REGISTRATION 1. To make sure that all attendees are well accounted for. MakScie received their training kits and
TRAINER AND Laptops and
7-Nov-18 8:00 AM and 2. To make sure that all attendees understood the course of Microbiology undesrtood how the training
ATTENDEES Projector
ORIENTATION the training. Lab will go and understood the
house rules
TRAINING: Safe
1. Define safe practices inside the microbiology laboratory All Attendess will be aware of
Practices for the TRAINER AND Laptops and
9:30 AM 2. Be aware that all laboratory research has risks and that Safe Practices for the
Microbiology ATTENDEES Projector
careful planning and preparation are required microbiology laboratory
Laboratory
1. To acquire skills in proper usage of Ultra Low Freezer.
All attendess acquired skills
TRAINING: Ultra TRAINER AND 2. To know the proper procedure on how to Protect your
10:00 AM Ultra Low Freezer needed in using Ultra Low
Low Freezer ATTENDEES samples with the right, energy-efficient ultra-low temperature
Freezer
freezers
12:00 NN LUNCH BREAK
1. To acquire skills in proper usage of Digital PCR.
TRAINING: Digital TRAINER AND All attendess acquired skills
1:00PM 2. To know the proper procedure on how to measure the Digital PCR
PCR ATTENDEES needed in using Digital PCR
amount of DNA or RNA present in a sample
1. To acquire skills in proper usage of Biosafety Cabinet. All attendess acquired skills
TRAINING: TRAINER AND
3:00PM 2. To know the practice of working safely with infectious Biosafety Cabinet needed in usingBiosafety
Biosafety Cabinet ATTENDEES
microorganisms. Cabinet
Microbiology Laboratory Training Matrix
DepEd Makati
Day 2
PERSONS MATERIALS
DATE TIME EVENT OBJECTIVES VENUE SUCCESS INDICATOR
INVOLVED NEEDED
8-Nov-18 8:00 AM Assembly Time
1.To acquire skills needed for the quick and accurate
determination of the mass of a sample using an analytical
balance.
MakScie All attendess acquired skills
TRAINING: TRAINER AND 2. To know the common sources of errors which affect the
8:30 AM Microbiology Analytical Balance needed in using analytical
Analytical Balance ATTENDEES weight of a sample being measured using the analytical
Lab balance.
balance.
3. To use the balance to demonstrate the need to dry
samples and standards prior to analysis.
1. To acquire skills needed for incubating microbes at the
temperature optimum for its growth.
TRAINING: TRAINER AND 2. To know how to maintain a constant temperature All attendess acquired skills
10:00 AM Incubator
Incubator ATTENDEES specifically suitable for the growth of a specific microbe. needed in using incubator.
3. To use incubator to demonstrate operation of setting the
desire temperature required.
12:00 NN LUNCH BREAK
 1. To acquire skills needed for the proper usage of the
autoclave in sterilizing materials.
TRAINING: TRAINER AND All attendess acquired skills
1:00 PM 2. To know the important use of autoclave in the laboratory. Autoclave
Autoclave ATTENDEES needed in using autoclave.
3. To use autoclave to demonstrate the proper preparation
of materials to be sterilize.
1. To acquire skills in proper using of shaker to mix the
components
2. To know the important use of shaker in laboratory
TRAINING: TRAINER AND All attendess acquired skills
2:30 PM experiments Shaker
Shaker ATTENDEES needed in using shaker.
3. To use shaker to demonstrate it's container capacity,
speed of mixing, variability of speed, liquid viscosity it can
handle.
TRAINING: TRAINER AND 1. To acquire skills in proper usage of centrifuge All attendess acquired skills
4:00 AM Centrifuge
Centrifuge ATTENDEES 2. To know the different application of centrifuge in laboratory needed in using centrifuge.
Microbiology Laboratory Training Matrix
DepEd Makati
Day 3
PERSONS MATERIALS
DATE TIME EVENT OBJECTIVES VENUE SUCCESS INDICATOR
INVOLVED NEEDED
9-Nov-18 8:00 AM Assembly Time
1. To acquire skills in proper usage of Inverted Microscope
TRAINING: with a mounted moticam MakScie Inverted All attendess acquired skills
TRAINER AND
8:30 AM Inverted 2. To use Inverted microscope to observe the specimen Microbiology Microscope needed in using inverted
ATTENDEES
Microscope living cells or organisms at the bottom of a petri dish or Lab Laptop microscope.
tissue culture flask
1. To acquire skills in proper usage of Vortex Mixer.
TRAINING: Vortex TRAINER AND All attendess acquired skills
11:00 PM 2. To use Vortex Mixer to demonstrate mix small vials of Vortex Mixer
Mixer ATTENDEES needed in using vortex mixer.
liquid
12:00 NN LUNCH BREAK
1. To acquire skills in proper usage of Rotary Evaporator All attendess acquired skills
TRAINING: TRAINER AND
1:00 PM 2. To use rotary evaporator to demonstrate the process of Rotary Evaporator needed in using rotary
Rotary Evaporator ATTENDEES
removing solvents from reaction mixtures evaporator.
TRAINING: TRAINER AND 1. To acquire skills in proper usage of Fume hood inside the All attendess acquired skills
2:30 PM Fume Hood
Fume hood ATTENDEES laboratory needed in using fume hood.
Closing TRAINER AND
3:30 PM
Ceremony ATTENDEES