CATEGORY

Drawings/Diagrams:
Participation:
University of San Agustin Time Bound
SENIOR HIGH SCHOOL DEPARTMENT &Appearance/Organization/
Preparedness:
General Luna Street, Iloilo City, Philippines, 5000
Conclusion:
Tel. No. (+63-33) 337-4841 to 44
Analysis:
AVERAGE/FINAL SCORE:
GRADE 12- General Biology 2 LABORATORY REPORT

Gas Exchange in Animals and Plant

(ACTIVITY#2)

Group #: ____ Strand/Year & Section: ______________

Leader: _______________________________________________ Date: ______________________________
(when the activity was performed)
Member/s: _____________________________________________
_____________________________________________
_____________________________________________
I. Objective:
1. To demonstrate the effect of temperature on the breathing of fish
2. To demonstrate the release of oxygen and determine the location of stomata on the leaves of various plants.

II. Introduction
Where are stomata located?
How do plants respire? You have learned that plants use carbon dioxide to produce oxygen through process of
photosynthesis. For this to happen, there are tiny openings in the leaves called stomata. That allow the exchange of gases; carbon
dioxide gets in oxygen gets out. Where stomata mostly located? Are they located in same area in all types of leaves?

III. Materials Needed:

Request following materials in Biology Stockroom: (by group)

 Water heater(1 water bath and 1 hot plate), 250 ml beaker (3pcs.), laboratory alcohol thermometer (1pc.), petri dish
(2pcs.), tap water (1 liter)

Bring the following materials: (by group)

 2 gold fish, Ice cubes or cold water, small leaves of mayana (2 pcs.), pansit-pansitan (2 pcs.), and kalamansi (2 pcs.)
 Old newspaper, tissue or rags, masking tape for labeling

IV. Procedure

Effect of Temperature on Gas Exchange in Animals

1. The first beaker will serve as the control setup. Fill the first beaker with fresh water. Record the water temperature.
2. Fill half of the second beaker with fresh water. Get the initial temperature.
3. Slowly add lukewarm water to the second beaker until the temperature becomes 200C.
4. Put the gold fish to each beaker. Observe for 1 minute per different temperature.

How Plants Give Off Oxygen

1. Fill the bottom and the cover of the petri dish with tap water.
2. Immerse the leaves, one of each kind in three Petri dishes.
3. Observe what happened after 5 minutes.
 University of San Agustin
SENIOR HIGH SCHOOL DEPARTMENT
General Luna Street, Iloilo City, Philippines, 5000
Tel. No. (+63-33) 337-4841 to 44

V. Documentations:
How Plants Give Off Oxygen

Leaves Name Before After 5 minutes

1. Mayana

2. Kalamansi

3. Pansit-pansitan

VI. Data and Observations:

How Plants Give Off Oxygen

Leaves Name Observations

1. Mayana
2. Kalamansi
3. Pansit-pansitan

Effect of Temperature on Gas Exchange in Animals

Breathing Initial 20oC 25oC 30oC 35oC

Rate/Minute Temperature

Goldfish
Control Setup none none none none
A

Goldfish
Experimental
Setup
B

VI. Analysis:

Effect of Temperature on Gas Exchange in Animals

1. What is the importance of the control setup?

2. Was there an increase in the breathing rate of the goldfish in control setup?
3. What happened to the breathing rate of the gold fish when you increased the temperature of the water in the second beaker?
4. What does an increase in the breathing rate indicate?
5. How do you relate this activity with fish kills that happen in most bodies of water?
 University of San Agustin
SENIOR HIGH SCHOOL DEPARTMENT
General Luna Street, Iloilo City, Philippines, 5000
Tel. No. (+63-33) 337-4841 to 44

How Plants Give Off Oxygen

1. What did you observe after 5 minutes

We observe that when the leaves are in the water within 5 minutes, many bubbles came out of the leaves.
2. Where did the bubbles come from?
The bubbles came from the leaves within 5 minutes.
3. How does the number of bubbles produced differ in each leaves?
The difference of the leaves in terms of bubble producing is the size and structure of the leaves.
4. What can you infer about the structures of the different leaves?

5. Explain how the natural location of the stomata, which is underneath the surface of most leaves, helps in conserving eater?

VII. Conclusion/Summary:
(answers the objective of the activity and relate the result to personal life experience or realizations)

Based on our experiment, the temperature affect the breathing of the fish, because when we put the goldfish in warm temperature,
the tail of the fish and the fins are moving quickly unlike in the normal tap water, the fish move calmly.

In Gas exchange on plants, based on our experiment the stomata is located some in the stem of the leaves and some are in under
the leaves. Another is the release of the oxygen on the leaves are also in the stomata but near the stem based on our experiment
because when we put the leaves on water, majority of bubbles came from the stem and also that is the location of stomata. Oxygen
is inside the leaf, when you put it in water, its oxygen will find a way out resulting to bubbles. Like when you are underwater with
your goggles, when you breathe out underwater, air will come out resulting to bubbles. So as we see the result of the different gas
exchange, especially on plants, same with us humans, also they need oxygen to grow and we need to protect them because plants
also provides us oxygen.

Please note: report should be printed in short bond paper, single space and follows the font size and style of this template