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INTRODUCTION
Cucumber Bettle is a common name given to number of two genera beetles both in
Chrysomelidae Family.
Heavy infestations of adult cucumber beetles can destroy stems, cotyledons, and
emerging leaves of young adult plants. Cucurbitacinas, which act as feeding stimulants, are
especially elevated in the cotyledons, leaves, stems, blossoms, and fruits of more mature plants
can be damaged, resulting in reduced yield and unmarketable fruit. Excessive feeding damage to
young squash and pumpkin plants can result in reduced yield. More mature plants can tolerate
feeding damage with little effect on yield. Root and lower stem feeding by cucumber beetle
larvae increases the incidence of certain diseases, but the importance of such damage has not
been quantified.
Adult cucumber beetle and some other plant-wounding insects also transmit bacterial wilt
to cucurbits. Cucumber and melon spices are most susceptible to bacterial wilt, squash and
pumpkin less so, and watermelon is not affected by the disease. Observations over the past few
years in New York, New Jersey, Delaware and Pennsylvania, however, suggest that this disease
may not be as widespread in the northeastern United States as one perceived. It may also be
cyclic in nature or important within some areas but not others. The bacteria may be transmitted
mechanically between plants through contact with mouthparts or feces of contaminated beetles.
Further study will be required to determine the mechanism by which the bacteria are transferred
and what proportion of beetles carry the bacteria .wilted plantsor their runners may indicate the
presence of bacterial wilt. Affected runners appear dark green at first, then become necrotic as
the wilt during the heat of the day but recover overnight. young pumpkin plants may wilt and die
within two weeks of the first symptoms, whereas summer squash may remain healthy and
productive for weeks following the first symptoms.to determine if a plant is infected with
bacterial wilt, press together two freshly cut sections of a stem and slowly pull them apart. if a
“stringy” sap (bacterial growth and associated resins) extends between the cut ends, the plant has
bacterial wilt. cucumber beetles also vector viruses such as cucumber mosaic but to doso much
Cucumber beetles adults over winter protected locations such as in woodland lifter and
under soil. Early in the spring they feed in blossoms and leaves of cultivated and wild host
plants. In new york they invade plantings of cucurbits in about mid-June just as the crop is
emerging, and their numbers remain relatively high through mid-July. One in cucurbits, the
female beetles lay groups of eggs at the bases of plants. These eggs hatch and produce larvae that
develop for 2 to 4 weeks on the roots, pupate in the soil, and appear as adults in early to mid-
August. This generation of adults remains in relatively high numbers until mid-to late September
and then overwinters. The spotted cucumber beetle migrates to the northern United States and
Canada each year; some individuals appear very early in the season in New York, however,
cucumber beetles begin to build in number in cucurbits in mid-August. Their life cycle resembles
that of the striped cucumber beetle, except that most individuals probably do not overwinter in
northern latitudes.
To control this pest, many farmers use pesticides that are expensive and affecting the
non-target organism, including human beings. If farmers keep I n buying commercial pesticide
agricultural chemicals,” says Roy C. Alimoane, Director of the Davao-based Mindanao Baptist
Rural Life Center (MBRLC) Foundation Inc. people who consume chemical-laced vegetables
risk their lives since chemicals are not always dissipated, saysAlimoane. Generally, chemicals
The Geneva-based World Health Organization report these people are poisoned by
pesticides every minute around the world. All in all, about 10,000 die annually because of
pesticides. Report shows that 62% of pesticides sold in the Philippines are insecticides of these,
46% are applied to rice and 20% to vegetables. Insecticides had become one of the major
expenses of farmers that account for about 40% of total production cost (Tacio, 2009).
“Natural pest controls like the botanicals are safer to the user and the environment
because they break down into harmless compounds within hours or days in the presence of
sunlight,” wrote Eric Vinje of Planet Natural. “They are also very close chemically to those
plants from which they are derived, so they are easily decomposed by a variety of microbes
Feeding by adult striped cucumber beetles and possibly the other beetle species
predisposes plants to gummy stem blight (causal fungus didymella bryoniae); beetles can also
Tubli is a rambling climber, with branches covered with brown hairs. Leaves are pinnate
and 30 to 50 centimeters long. Leaflets are usually oblong, 9 to 13, when matured smooth above
and subglaucous and silky beneath, 10 to 15 centimeters long, and about half as broad. Racemes
are lax, 15 to 30 centimeters in length with reddish flowers in stalked clusters. Insecticides had
become one of the major expenses of farmers that account for about 40 percent of total
production cost.
But lately, the harmful effects of inorganic pesticides to environment, humans ,and
livestock prompted various countries worldwide to ban the use of this pesticides and look for
promotes balanced and self-regulated agricultural systems. It also less harmful to the
Researchers at the University of the Philippines in the Visayas, West Visayas State
University and Panay State Polythecnic University jointly implemented a project to develop a
The product will be produced commercially and sold to farmers at affordable price. The
Philippine council of agriculture , forestry and natural resources research and development , an
Potential plants for the project were selected based on availability, high bioactivity and
endecimicity. Selection was also based on the plants whether such ingredients already known
tubers of kayos (Dioscorea hispida) were used for the development of the natural pesticides. The
plant materials were collected from Mamburao in Capiz, and Miag-ao town in Ilo-ilo. Most of
compounds isolated from tubli are isoflavonoids. The roots of Derris elliptica , which is closely
The tubers of D. hispida contain a poisonous alkaloid called dioscorine that paralyzes the
nervous system. It also has diosgenin, which generally occurs in combined form as glycoside
of steroids.
This study would like to determine the effectiveness of Derris elliptica Benth (Tubli) root
The future researchers will not have trouble in terms of using Derris elliptica Benth
(Tubli) root extract. They will no longer use the commercial pesticide and this will not affect
their health.
Agriculturist:
This study will give some agriculturist some insights about the use of Derris elliptica
Benth (Tubli) root extract. It is an alternative to chemical pesticides to help eradicate cucumber
beetles. This will also motivate them to promote Derris elliptica Benth (Tubli) root extract to
Environment:
The result of this study will help to maintain the balance of the ecosystem. The researcher
did not use synthetic chemicals that can kill non-target organisms.
Scope and Delimitation
This study is only limited to the stem extract of Derris elliptica Benth (Tubli) root extract
applied to eliminate cucumber beetle. This is conducted to know the effectiveness of Derris
elliptica Benth (Tubli) root extract in eliminating cucumber beetles. Other insects and pest are
not included in the study and only Derris elliptica Benth (Tubli) root extract serves as
independent variable.
Conceptual Framework
This paradigm will determine the effectiveness of Derris elliptica Benth (Tubli) root
extract in eliminating cucumber beetle. Conceptual framework shows that Derris elliptica Benth
(Tubli) root extract as the independent variable that will contribute to increase the Mortality rate
Hypothesis
Definition of Terms
Chrysomelidae- the insects of the beetle family chrysomelidae are commonly known as leaf
beetles.
Cotyledons- an embryonic leaf in seed-bearing plants is more of which are the first leaves to
Necrotic- the death of tissues from svere injury or disease, especially in alccalized are of the
body.
This chapter shows what is already known about the research topic as a whole.
Locale Literature
The roots of tubli contain rotenoids, a highly potent insecticidal chemical which is widely
used for the control of a variety of insect pest in the home, on domestic animals and on the field.
It also widely used as fish killer. On the field crops, it has a short (3-5 days)residual toxicity and
the residues are non-toxic to man. In recent years, Filipino scientists have found many plants to
have insecticidal properties. One of them is “tibanglan,” known locally in Mindanao as “tubli.”
coleopeterous insects.
From Derris sp.: A Review on the extraction Method, Toxicity and the Field effectiveness, bio-
pesticides are natural occurring substances that control pest and derived either from plants or
microorganism it play a vital role in coping with adverse effect arise due to massive used of
synthetic pesticides and development of insecticide resistance of some insect groups in crop
production. Thus, Derris species from leguminose (Fabacea) family is a climbing plant of
Southeast Asia that was well-known for its environmental bioactive compound is used one of the
effective insecticides. Rotenone was one of bioactive constituents extracted from derris plant that
was used as agricultural pest control due to its strong paralysis action (knock-down effect) on
cold blooded animals. Moreover, it has a lethal mechanism in inhibiting respiratory enzyme of
The test conducted by the Laguna-based Ecosystems Research and Development Bureau
included leaf rollers and fruit worm of okra, stem borers of eggplant, white aphids, corn weevil
and larvae or tussock moth. Result of the study showed that 50%, 25% and 10% of “tubli” sap in
water can kill samples in 20, 45, and 120 minutes, respectively.“Tubli” has an ancient reputation
as botanical pesticide. Ethnic groups in some parts of the country have long been using “tubli” as
The insecticidal properties of “tubli” were first discovered in 1848 when the plant was
first used against the nutmeg caterpillar. In 1940, the United States was importing 2,700 metric
Agricultural experts say “tubli” is very effective against aphids, beetles, borers, the
diamond back moth, fruitflies, thrips, cabbage worms, fleas, flea beetles, lice, loopers, mites,
According to Philippine Medical Plants, Roots are insecticidal; rotenone from roots is
raw material for insecticides against plant pests. Since early times, the root has been used as fish
poison in the Philippines have used the root decoction as an insecticide. Rotenone has also been
found effective against plant lice, leaf beetles, aphids, flies, caterpillars, ticks, chicken lice, red
spiders and other insects. In the Philippines, cattle have been reported to die after eating the
leaves because of toxicity, rotenone as insecticidal is being phased out in many eco-conscious
countries.
Furthermore, according to a study conducted by Amistad, Mendoza and Sumilhig entitled
the Effectivity of Tubli Plant (Derris Elleptica Benth) root extract as mosquito larvicide
compared to a commercial larvicide, the tubli plant were obtained in one of the researchers
residence at Negros Occidental. The breeding of day-biting household mosquito larvae was done
at the residence of one of the group members located at Marigodon, Lapu-lapu City, the
preparation of the root extract was conducted at Cebu Doctors University. A population of 270
day-biting household mosquito larvae were the subjects of the research study. 90 wrigglers were
randomly allocated into 3 groups for 3 trials: experimental group(30),positive control group(30)
The researchers collected30 day-biting mosquito larvae per trial. The wrigglers were
randomly selected and were each placed in a transparent plastic container. They were divided
into three groups , each having 3 trials. The negative control group which was treated with the
100% tubli plant(Derris Elleptica Benth.) root extract. The number of dead larvae were counted
and recorded in three time intervals after the treatment. The result shows , the experimental
group treated with 100% tubli (Derris Elleptica Benth)root extract showed a perfect number of
deaths ( 30 wrigglers). The positive control group treated with the commercial larvicide also
showed a great number of deaths (26-29 wrigglers). The negative control group with only rain
water showed no deaths of larvae. Based from the data gathered , the researchers concluded that
extract from tubli plant (Derris Elleptica Benth.) was more effective than the commercial
larvicide.
National Literature
mosquito larvacidal efficacy of rotenone extracted from Malaysia Derrissp”. Rotenone is a bio-
active compound extracted from Derris elliptica (locally known a s tuba plant). It has long been
used as bio-pesticide, which is more environmental friendly than the commercially available
pesticides and has the potential to be used in eliminating mosquito larvae. Therefore, the
document of this study is to determine the mosquito larvicidal activity (LC50) through the usage
The rotenone liquid crude extract was extracted using normal soaking extraction method.
Two different solvent ratios were used to extract rotenone namely: (A) methyl chloride methanol
(1:1) and (B) methyl chloride: methanol (1:9). The extracts were concentrated using rotary
performance liquid chromathography analysis (RP-HPLC) and biological activity study. Next,
the diluted extracts were subjected to the biological activity (LC50) treatment for 6 hrs. The
results showed that the concentrated liquid crude extracts of methyl chloride: methanol (1:1)
which contained the highest rotenone content produced the lowest treatment concentration of
0.024 mg/ml to achieve 50% mortality within 3 hrs of treatment (p<0.05). The rapid mortality (as
indicated by the LC50) of the mosquitoes’ larvae against rotenone extracted from Derris plant
roots has proven that it has the potential to be used as larvicide to control vector-borne diseases
Research Methodology
The purpose of this chapter is to explain in detail the research methods and the
methodology implemented for this study. The chapter will explain first the research design,
population and sampling plan and the research locale. This will be followed by the schematic
diagram, research instrument and statistical treatment. It concludes with a brief discussion on the
ethical considerations and data gathering procedure posed by the research methodology.
Research Design
The research makes use of a quantitative research strategy in the sense that it is an
inquiry into social problem, explain phenomena by gathering numerical data that are analyzed
strategy is particularly applicable for the purpose of the study, where determining the
effectiveness of Tubli (Derris elliptica) root extract to eliminate cucumber beetle (Diabrotica
undecimpunctata).
Population and Sampling Plan
According to Fraenkel and Wallen (1993:79), population is the group to which the results
of the study are intended to apply. The population in this study was the number of cucumber
which is 30.
In this study the researchers were no longer look for respondents. The researchers used
Research Locale
The experimentation and collection of materials needed in the study took placed in the
residence of Rogelio H. Surdiaz Sr. at Purok 3 Lumbangan, Zamboanga City, residencial of one
of the researchers.
Schematic Diagram
Getting of Tubli Roots
Preparation of Set-Ups
Testing of Set-Ups
Experiment
Observation
Figure 1.The Diagram shows the step by step process of our research. It starts with getting of
Tubli Roots and its extract, then, followed by getting of Cucumber Beetle. Afterwards, the
preparation of set-ups. Then, testing set-ups and lastly, is the observation to get the exact data
For the purpose of this study the researchers used observation to attain valid and reliable
result.
Statistical Treatment
and match to our study. The researchers uses this statistical method to gather and determine the
effectiveness of Tubli (Derris elliptica Benth) Root Extract on eliminating cucumber beetle and
also this statistical method is used to get the exact and valid result of the study.
Ethical Consideration
This research will be done with confidentiality on the data that will be collected. The
researchers will present the research and its approval will grant by the intellectual property of
right given to the researchers and to the Maria Clara Lorenzo Lobregat National High School
Procedure
The researchers collected and get tubli roots at Brgy. Lumbangan, Zamboanga City. After
getting the roots, we undergo the extraction process. Afterwards we come up with a 100ml root
extract and put it in a 100ml bottle spray. On the other hand, we get cucumber beetle and place it
inside the set-ups. Afterwards we do the experiment where, we sprayed the cucumber beetle and
Analysis of Data
This chapter discusses the results of the data interpreted, analyzed and treated
statistically.
Interpretation:
The table shows that the mortality rate of the cucumber beetle is higher when we spray
the Tubli root extract in the afternoon.
Interpretation:
Table 1.3 Mortality Rate of Cucumber Beetle in Third day of experiment
Interpretation:
Table 1.4 Mortality Rate of Cucumber Beetle in the fourth day of experiment
Interpretation:
Table 1.5 Mortality Rate of Cucumber Beetle in the fifth day of experiment
This chapter presents the finding and conclusions drawn and the recommendations made
as an outgrowth of this study.
Findings:
The tubli(derris elliptica benth) root extract is not effective in eliminating cucumber
beetle.
Solving the average mortality rate of cucumber beetle using z-test, result shows that there
There is no significant difference between tubli root extract and cucumber beetle that
Conclusions:
It find out that tubli (derris elliptica) root extract is effective in eliminating cucumber
beetle. It shows that tubli root extract is more and cucumber beetle and it implies that the null
hypothesis is accepted.
Recommendations:
Needs further studies on the amount and content of tubli root extract to achieve the
successful result.
Internet Links:
http://jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/5942
http://www.herdin.ph/index.php/partners?view=research&cid=52874