MULTIPLATE lgA - lgG'lgM RID

Determination of the lgA - lgG - tgM protein, by radial immunodiffusion plate

TEST SUMMARY the controls. A straight Ene, with an intercept in

-GEamineO protein, diffusing in agarose gel the range 10-11 mm, shotdd be obtained.
Sample values are determined by interpolation.
containing a specific antibody will form an
immuno-Complex, visible as a ring around the Procedure 2
well. The ring diameter is direct proportional to Construct a curve that plots the square of the
the concentration of the analysed protein. The precipitating ring versus the
logarithm
lnterassay (n= 20) Mean SD (ms/dl) CV%
proportion corresponds to the diffusion time. ln concentrations of the contols The graph will be
'1302.32 21.90 1.68
tad, at tre end (72h for lgrA and lgG' 96h for a straight line only for lq! values. Sample 1

tSU), fre square ot dbmeter will be in linear Sample values are detemiined by interpolatirn- Sampb 2 18G1.52 6.23 1_35

iotiofion b tre oncenbatbn (procedure 1-3), Procedure 3

wtrde afrer shorter pedod of difr.tsion the square Read on encloeed reference table the
of diameter will be in a logarithmic proportion to @ncentration value corresponding to the
the concentration (procedure 2). ln both cases, precipitating ring dameter. The ring value is
a calibration curve should be constructed, using obtained from lhe control serum (that has to be
at least three calibration points. However a used every time) which shouH have a
reference table is provided showing the relation confidence limil of 0.2 mm (from the reference
between any concentration and the end of the htet-asay (n= 2()) Man SD (ngl,d) cv%
table).
procedure. 1fI2-10 Lfi 225
Sarpb 1
NOTES
Sarple 2 26E.60 tl.(E 1.51

protein' After 72 h ot 96 h the difusi'n d the Heasure's limit

REAGENTS protein at any concentration is compbled' For lgA 70 - 1050 mg/dl
i lgG 300 - 3500 mg/dl
lgM 40 - 500 mg/dl

REAGENTS PREPARATTON AND SToRAGE b dbaHe to read again afler 3/5 hours' lf the WASTE DISPOSAL
dareter is still the same it is possibleto set the
in" I"rg""t. are stible until expiration date on correntation, on the contrary, if the diameter is laboratories. Please consult local regulations for
in"r"o"iitpr"rervedhorizontal at2-8"C. ftrent, ring should be remeasured after a a correct waste disPosal.
ftrts3/5 hours.
Stability after opening: two weeks if, after the {, EXPECTED VALUES
first use, is preserved werr croseo GALIBRATIoN a1 i+;C' , , ,',
2.-t!849,: ar
Tt* #le can be trsed for turther - - EE@$ffo.T.purpose the following
it@{.qEitv 3oc 80o- 1800 Elgtd
irt dngfreaccuracybyacon$olserum' contol':Eithid
based control sera are araible:
human
rdrvr 6o - 280 mg/dl
REQuIRED Bur Nor SUPPLIED
lC0O400 Multiset multiParametric 4 x l ml
MATERTALS PACKAGING
-""" (for o-1 acid Glycopr&in, c3' c4, lgA, lgG, coD RK01050
laboratory instrumentation.
lgM and Transferrin) Multiplate lgA -lgG -lgM 3 x 5 wells

PREGAUTIONS REFERENCES
ntiagvl l( I I raJ, uvr rrqrr I evr
lt is suggested to Methodscomparison --- and a commerciallv
I I
ffi,istry.2:23s(1965)
94 : 84 (1965)
oilsirvative components. Fahey & coll.- J. lmmunol.
t;iil:;f;|; i[-;;;idi"s'" .".t.",i"riitii rri" A comparison between LTA
gave the following results on
product
and swallow. "Good lvailable
70 samples:
MANUFACTURER
F"rto* in" test according to the general
LTA s.r.l.
Laboratory Practice" (GLP) guidelines' lgA LTA = x Via Milano 15/F
lgA competitor = Y 20060 Bussero (Milan) ITALY
PROCEDURE Tel: ++39 02 95409034
n=70
nemove jtre ptate from its envelope and leave Fax: ++39 02 95334185
to stand at room temperature for few minutes =1,001x+2,964 r=0,971€ e-mail: info@ltaonline.it
so that any condensed waler in the wells can lgG LTA = x
Website: htto:/,ww.ltaonline.it
evaporate. Fill the wells with 5 pl of sample lgG comPetilor = Y SYMBOLS
and/or controls and wail it has been completely n=70
adsorbilg before handling the plate. Clgse the @ For in vitro diagnqstic use only
plate and place it in a moist c-lramber. Wait the =0,9835x+23,16'l r=0,99942
E Lot ofmanufacturing
required incubation Period:
Foi lgA and lgc 72 hours for the procedure 1 or
lgM LTA = x
E Code number
3, 1 8 hours for the Procedure 2.
For lgM 96 hours for the procedurc 1 ot 3' 24
lgM competitoi =
n=70
Y
t Storage temperature interval

hours for the Procedure 2.

To quicken analysis time it is possible to put the
=0,989x+2,17 r=0,9999 I Expiration date

plates in a thermostat. Precision A Warning, read enclosed documents

RESULTS INTERPRETATION
E Read the directions

masurelFe precFitating ring to the nearest 0.1 & Biological risk

Mod. 01.00 (ver 1'2 - 13/09/2005)
mm, after the required period according to the,
procedure should be followed, and the type of
protein.
lnter-assay (n= 20) Mean SD (ms/dl) CY Yo ((
Procedure 1
299.62 6.19 2.07
Sample
Construct a curve that plots the square of the
1

precipitating ring vergus the concentrations of Sample 2 454.61 6.68 1.47