2019-2020

Bacterial Contamination of drinking

water testing by sulphide ion

NAME:- AKASH AKSHAT, ANISH,ANJALI

ROLL NO :- 01,02,03,04
Class:- XI ’Sci’
Submitted to:- Sunil Vodela

SCHOOL:- K.V. HAPPY VALLEY | Happy Valley

This to certify that this project on the topic
‘checking the bacterial contamination of
drinking water testing by sulphide ion’ has
been successfully completed and submitted
by Mr. Akshat Dixit of Class XI ‘Science’
and Kendriya Vidyalaya Happy Valley
Shillong, during the session 2019-2020
under the guidance of and supervision and
has been submitted to the Department of
Chemistry.
Date:

Internal Examiner External Examiner

Principal
This to certify that this project on the topic
‘checking the bacterial contamination of
drinking water testing by sulphide ion’ has
been successfully completed and submitted
by Mr.AKASH BISWASof Class XI ‘Science’
and Kendriya Vidyalaya Happy Valley
Shillong, during the session 2019-2020
under the guidance of and supervision and
has been submitted to the Department of
Chemistry.
Date:

Internal Examiner External Examiner

Principal
This to certify that this project on the topic
‘checking the bacterial contamination of
drinking water testing by sulphide ion’ has
been successfully completed and submitted
by Mr. ANISH KUMAR of Class XI ‘Science’
and Kendriya Vidyalaya Happy Valley
Shillong, during the session 2019-2020
under the guidance of and supervision and
has been submitted to the Department of
Chemistry.
Date:

Internal Examiner External Examiner

Principal
This to certify that this project on the topic
‘checking the bacterial contamination of
drinking water testing by sulphide ion’ has
been successfully completed and submitted
by Miss.ANJALI MAHATO of Class XI
‘Science’ and Kendriya Vidyalaya Happy
Valley Shillong, during the session 2019-
2020 under the guidance of and supervision
and has been submitted to the Department
of Chemistry.
Date:

Internal Examiner External Examiner

Principal
I Akshat Dixit, AKASH BISWAS, ANISH
KUMAR, ANJALI MAHATO of Class XI
Science, of Kendriya Vidyalaya Happy
Valley Shillong-07 performed this
investigatory project under the guidance of
my chemistry teacher Sunil Vodela.

I am very thankful to my teacher and

parents who helped me in my investigatory
project. Without whose motivation and
help the successful completion of this
project would not have been possible
1. INTRODUCTION
2. AIM
3. REQUIREMENTS
4. PROCEDURE
5. OBSERVATION
6. RESULT
7. CONCLUSIONS
8. PRECAUTIONS
9. BIBLIOGRAPHY
The sulphide ion test for bacterial
contamination is good. In surface waters,
hydrogen sulphide is formed under oxygen
deficient conditions. Hydrogen sulphide is also
produced from the decomposition of sulphur
containing organic compounds. The
concentration of sulphide ion becomes
significant only at pH 10 or above.
Under acidic conditions, the concentration of
hydrogen sulphide predominates. Hydrogen
sulphide is a weak acid, which ionizes to yield
hydrosulphide(HS-) and sulphide (S2) ions.
Hydrogen sulphide is highly toxic to fish.
Concentrations of total sulphide as low as 0.01
mg/L make the water uninhabited fish culture.
Hydrogen sulphide also reduces the aesthetic
value of the water body due to foul colour. The
bacterial contamination can be tested using
H2S strip.
Presence of pathogenic bacteria in water can
be detected by testing the sulphide content of
water. The presence of sulphide ions in water
is an indicator of: i) High pH above 10.ii)
Sulphide producing bacteria iii) Diminished
oxygen concentrations Conducting the
sulphide ion test:- The sulphide ion test is
conducted using an Hydrogen sulphide strip
(H₂S). - The H₂S strip is dipped into the water to
be tested for 14 to 16 hours. - The H₂S strip
turns black if sulphide ions are present in the
water. The black colour is due to the ionization
of the H₂S.
To test the contamination of drinking water by
bacteria be checking the sulphide ion
concentration and find out the cause of
contamination.
Apparatus Required
1. Hydrogen Sulphide (H2S strip)
2. Beaker
3. Drinking water
4. Thermometer

CHEMICALS REQUIRED

1. Hydrogen Sulphide (H2S)

 This experiment is to be done in an
incubator

 Add about 250ml of water in a beaker.

 Dip the H2S strip in the beaker with
water.
 Heat the beaker for about 35 degree
centigrade
 Check the temperature (temperature
should be at 35 degree centigrade)
 Keep it in incubator for 14-16 hours of
time.
 Check the result if the water turns black
then it is unfit for drinking / highly
contaminated for drinking or for human
use.
 The sulphide ion test for bacterial
contamination is good. In surface
waters, hydrogen sulphide is formed
under oxygen-deficient conditions.
Hydrogen sulphide is also produced
from the decomposition of sulphur
containing organic compounds. The
concentration of sulphide ion becomes
significant only at pH 10 or above
Source of Water Result
Water from the Water is fit for
tap of house at drinking.
madanrting
Water from the Water is unfit for
tap of happy drinking
valley houses
Spring water Water is fit for
drinking
River water Water is not fit
for drinking
By the experiment done the result
says that at some place water is fit for
drinking at some places whereas it is
unfit for drinking at some places.
Especially water is unfit for drinking
from river bodies.
 Water is mostly unfit for
drinking from river.
 Water is also unfit for drinking
at some of the water that come to
houses.

 Therefore we should purify the

water before drinking. And we
should not drink water from
random places.
The handling precautions should be
numerous and multi-level. They also need
to be adapted to the volume of H2S that
you will have inside the lab and the extent
of the research effort that you plan that
involves H2S. It is one thing to run a
couple of experiments with small volumes
of H2S and it is another to use large
numbers of moles of H2S for extended
periods of time.
For small volumes and limited
experimentation, working with everything
inside of a well functioning fume hood
along with a portable monitoring system,
air packs, and notification of "neighbours"
and an H2S training program for everyone
who MIGHT get involved (include
neighbours), is an absolute minimum.
For large volumes of H2S and/or a
extended research program, you need to
have the area where the testing is
performed so well ventilated that all the air
in a room can be evacuated in just a couple
of minutes (for this assume that the
containment inside the hood will be
breached). There should be a fixed H2S
monitoring system located just above floor
level because H2S is heavier than air. This
monitoring system should be attached to
an alarm system (both audio and visual like
a fire system) with the readout located
outside of the containment area and an
alarm should activate the air evacuation of
the containment area. The air pack supply
should be located outside of containment
as should the buddy during any activity that
handles H2S. And the training of anyone
impacted by the testing should include the
operation of the H2S alarm system, use of
the rescue breathing equipment,
procedures for removing someone who
has been knocked down by the H2S inside
the containment area and EVERYONE
should be certified to perform CPR at least
every six months.
Finally, don't forget that you will need a way
to recover and neutralize any H2S that you
are handling. That goes for both the H2S
that is successfully contained in your test
as well as any H2S that is accidentally
released. For small H2S volumes for short
tests, you need to have the outlet of your
hood evaluated for exit velocity and stack
height. If you can push the H2S out fast
enough and high enough above the
building, then dispersion may be sufficient
for safe handling (once again remember
that it is heavier than air so it sinks). For
large volumes and extended research, you
need to have a commercial gas scrubber
attached to the exhaust system that is
designed to handle the air volume from the
emergency exhaust condition. These
systems can work using a number of
chemicals to scrub the H2S. I have worked
with systems based upon the use of
sodium carbonate and others that used
iron oxide particles.
1. Chemistry Lab manual
2. www.Brainly.in
3. www.Scribd.com
4. www.Meritnation.com
5. Ncert.nic.in
6. www.indiawaterportal.org
7. www.coursehero.com