TARLAC NATIONAL HIGH SCHOOL - MAIN LK007

INACTIVATION OF Aspergillus fumigatus RESISTANCE

BY SYNERGISTIC ACTIVITY OF
BIOSYNTHESIZED COPPER NANOPARTICLES
USING BANGKOK CALACHUCHE
( Adenium obesum) AND SELECTED
AZOLE MEDICATIONS

RESULTS
In this experiment, Aspergillus fumigatus strain was used as the fungi strain to determine
the synergistic activity of biosynthesized copper nanoparticles using Adenium obesum
and selected azole antifungals. The results were observed in three distinct periods. (12,
24, 36 hours) after isolating and allowing A. fumigatus to settle.

BACKGROUND OF THE STUDY 10mL Itraconazole 10mL Fluconazole

Dilution Dilution
Table 1. Measurements
Pathogenic infections still remain as one of the main factors for morbidity and mortality around of the solution per
the world despite the variety of effective medicines being produced as multidrug resistance treatment.
10mL CuNPs T1 (CuNPs- T3 (CuNPs- T5 (CuNPs)
has become an arising world health problem that continues to involve more harmful microbes
including Aspergillus fumigatus. A. fumigatus contributes to a large portion of clinical infections Itraconazole) (1:1) Fluconazole) (1:1)

requiring hospitalization and developing resistance against antibiotics poses a huge threat to
T2 (Itraconazole) T4 (Fluconazole)
consumers’ health.
The purpose of the study is to discover breakthroughs in nanotechnology in the field of medi-
cine, especially with the growing number of multi-drug resistant pathogens, such as A. fumiga-
TREATMENT Mean Initial Mean Final GROWTH
tus. The use of this technology has become widespread and is contributing greatly to the differ- A. fumigatus Eradicated A. INHIBITION
ent fields of science. It has been proven efficient with different nanoparticles that can be used colonies fumigatus (%)
in experiments. colonies
T1 (CuNPs- 66.33 56.33 84.92%
Table 2. Mean Growth Itraconazole)
Inhibition Rate of Treat- T2 70.66 34 48.11%
ments 1-5. (Itraconazole

statement of the problem

)
T3 (CuNPs- 78.33 63 80.42%
Fluconazole)
T4 85 42 49.41%
This study seeks to determine the synergistic activity of biosynthesized CuNPs using Adenium (Fluconazole
obesum and azole medications in the inactivation of Aspergillus fumigatus. Specifically, it aims )
to answer the following questions: T5 (CuNPs) 63.33 57.66 91.04%
1. Is there a significant difference after adding Copper nanoparticles in the Itraconazole 100mg
Chart Title
100
diluted solution? 90
91.04
2. Is there a significant difference after adding Copper nanoparticles in the Fluconazole 100mg 80 84.94
70 80.42
diluted solution? 60 Figure 1.
3. Is the Copper nanoparticles solution able to inhibit the growth of the A. fumigatus colonies? 50
40 48.11 49.41 Graphical
4. What recommendations can be drawn from the study?
30 comparison of
20 the results per
10
0 treatment.

METHODOLOGY
Series 1 Column1 Column2

1
> 2 conclusions and recommendations
PREPARATION OF MATERIALS AQUEOUS EXTRACTION OF There was a significant decrease in the A. fumigatus colony count in the CuNPs-Itra-

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(Leaf Materials, Azoles, Copper Sulfate LEAF MATERIALS AND DILUTION OF AZOLES conazole and Fluconazole-CuNPs treatments compared with the Itraconazole and
Pentahydrate) (Itraconazole 100mg and Fluconazole 100mg)
Fluconazole only treatments. The results showed 36.81% and 31.01% increase in the
>

effectivity, respectively. The Copper nanoparticles (CuNPs) were able to boost the
medicinal effect of Itraconazole and Flucnazole, an azole medication highly resisted
4 > 3 by the A. fumigatus strain, provided that they are combined with CuNPs.

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The Copper nanoparticles solution had the highest efficiency rate among the five
treatments, having a 91.04% decrease in the final colony count of the A. fumiga-
BIOSYNTHESIS OF COPPER tus strain. It can be concluded that the CuNPs solution is proven effective against
NANOPARTICLES the pathogenic fungi, A. fumigatus.
ISOLATION OF A. FUMIGATUS
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The researchers recommend further analysis and experimentation with different
azole medications that are resisted by different pathogenic fungi strains, including
5 A. fumigatus. They would also recommend that more studies be done using differ-
ent pathogens, especially pathogenic bacterial strains, medications that are resist-
ANTI-FUNGAL ASSAY AND
ed by these bacteria, and different nanoparticles
STATISTICAL ANALYSIS

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