Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
Coffee
Rinantonio Viani, Association Scientifique Internationale du Café (ASIC), 1802 Corseaux, Switzerland
Marino Petracco, illycaffé S.p.A, Trieste, Italy
Caffeine, the active principle of coffee, can being roasted and consumed as a brew, kahwa,
be removed from the green bean after soaking had conquered the whole Arabian peninsula and
in water, by dissolving it in a solvent, such as reached the Mediterranean Sea. During the 16th
dichloromethane, ethyl acetate, or supercritical century, coffee became very popular throughout
carbon dioxide. Adsorption on activated carbon the Ottoman Empire.
is also employed. In addition to caffeine, ca. The stimulating properties of coffee are de-
1 % in Arabicas and 2 % in Robustas, the dry scribed by the legends reported in Abdelkadir’s
bean contains ca. 50 % carbohydrates, 8–17 % manuscript, where strength to survive in the
lipids, up to 11 % proteins and amino acids, and desert or to stay awake and pray is restored
ca. 5 % minerals. During roasting, these com- to holy people by chewing the sweet berries
ponents form the typical coffee flavor, of which or by drinking a beverage prepared by roast-
more than 1000 components, many of them fra- ing the berries to make them softer and to re-
grant or sapid, have been identified. duce their bitterness. Leonhard Rauwolf (ca.
1540–1596), physician and botanist from Augs-
burg, was one of the first Europeans to describe
2. History [4] coffee as a drink “almost as black as ink” and
to give a vivid description of Aleppo’s coffee-
Unlike most cultivated plants whose primitive houses. Prospero Alpini (1553–1617), physi-
strains have long ago disappeared by genetic cian and professor of botany at the University of
erosion, wild coffee plants, related to the ac- Padua, drew the first picture of a coffee branch
tual cultivars of C. arabica, still exist in East from a tree seen in the garden of the Bey in
Africa in the region where the main species orig- Cairo. By way of Venice, coffee from Mocha
inated. The plant spread from the rainy forests conquered Europe in the 17th century. Coffee
around Lake Victoria to the Kaffa province in culture was introduced to the Malabar coast of
the Ethiopian highlands, north to the arid Sudan, India and to Ceylon toward the turn of the 16th
where its sweet red cherries, bunna, were picked century. By the end of the 17th century the Dutch
and chewed. Dried cherries are easily stored and had succeeded in cultivating coffee on a com-
transported; thus, their use reached the coastal mercial basis in Java; they broke the Mocha
area, where they are known as m’buni or buni, monopoly in 1711, when the first coffee pro-
and are still consumed sweetened and unroasted duced in the East Indies was auctioned in Ams-
on special occasions as a ceremonial food. terdam. Seedlings from a plant brought to Am-
Intense commercial and political exchanges sterdam in 1706 were distributed to the Euro-
across the Red Sea introduced coffee use to Ara- pean botanical gardens. Due to a state of war,
bia, and its culture may have started as early as the French waited until 1713, when a plant 5 ft
the sixth century in the fertile valleys of the coun- (ca. 2 m) tall was sent to Louis XIV, the Sun
try now known as Yemen. King. It was planted in the Jardin des Plantes
The advent of Mohammedanism, forbidding in Paris and described by Antoine de Jussieu.
the use of alcoholic beverages and encouraging By royal bequest it became the ancestor, with
exchanges through pilgrimages to Mecca, to- related plants from Amsterdam, of most of the
gether with the flourishing of the Arabian and coffee trees planted during the 18th century in
Persian civilizations, asserted the strength of the Western hemisphere, which eventually led to
coffee around the harbor of Mocha and its ex- the decay of Dutch supremacy in the trading of
tension eastward. The Persian scholars Rhazes coffee.
(850–922) and Avicenna (980–1037) mention The Swedish botanist Linnaeus, a friend of
the digestive properties of coffee, probably iden- the Jussieu family, classified the plant as Coffea
tified with bunchum by the former and called bon arabica in 1753. Caffeine [58-08-2], the active
or ban by the latter. ingredient of coffee, was isolated by Runge in
According to Abdelkadir, who in 1587 wrote 1820 from a few beans received from Goethe.
the first historical account dealing specifically The formula of caffeine, which had been de-
with coffee, the expansion northward appears to termined from combustion data by Pfaff and
have been slower. It was only by the second half Liebig in 1832, was established by Medicus in
of the 15th century that coffee, which was then 1875 as 1,3,7-trimethylxanthine, C8 H10 N4 O2 ,
Coffee 3
bor is available at harvest time, immature and in the sun. They are placed in layers up to 6 cm
ripe cherries are stripped off the branches and thick, turned regularly, and sheltered from rain
collected from the ground along with the over- showers and dew. The coffee dries in 2–3 weeks,
ripe and dry ones. The leaves and twigs are win- and the husks can then be removed mechanically
nowed away in the field. Often a water siphon, from the beans.
in which the heavier cherries sink, while leaves, The wet process, which uses water flowing
twigs, and dry cherries float and are diverted, down a slight gradient, was introduced by the
is used to carry out an initial grading of the Dutch in the West Indies during the first half of
cherries. The middle fraction containing the ripe the 18th century and is used where enough water
cherries is further treated by either the dry pro- is available and the climate is humid. Harvested
cess, in which the whole cherry is dried, or the cherries are handled in bulk if they can be pro-
wet process, in which the pulp surrounding the cessed in less than one day; otherwise, they are
bean is fermented and washed away before the stored under water to avoid overheating. Where
beans are dried in their parchment [12]. Raw the water supply is abundant, cherries are pre-
beans recently processed in either of these ways graded in a washing channel or a siphon tank.
are recognized by their color: dry-processed (or Where water is scarce, the cherries are supplied
natural) beans are yellow-green to pale brown, dry to the pulper. This reduces water consump-
whereas wet-processed (or washed) beans are tion by half (60 vs. 120 L/kg). Pulping is done
green to blue-green. by squeezing the cherries through slots in drum
or disk machines to separate the outer skin and
some of the fleshy pulp (the pericarp) from the
parchment coffee (the bean surrounded by the
endocarp). Parchment coffee is then sorted by
gravity, and floaters, light parchment, peaber-
ries (oval beans from cherries containing only
one seed), and heavy parchment are sent to sep-
arate tanks. The sticky pulp still adhering to the
parchment is loosened by fermentation in a tank,
either dry in the open for 24–48 h or under water
for up to 72 h, depending on the altitude. The fine
acid taste of some of the coffees grown at high
altitudes is attributed to the long fermentation
time. Adding pectic enzymes or alkali may ac-
celerate fermentation. Fermentation is stopped
by soaking parchment coffee in water.
Pulping and fermentation are sometimes
combined in the “aquapulper,” a pulper and
Figure 1. Transverse section of a coffee cherry containing demucilager, where the pulp is rubbed off the
two seeds (courtesy Dr. E. Dentan) parchment mechanically. All pulp residues are
Pericarp: “Skin” or exocarp, red (or yellow); “Pulp” or washed away in a channel where the remaining
mesocarp, yellow-white; “Parchment” or endocarp, pale
yellow
skins and whole cherries, which are lighter, are
“Silverskin” or spermoderm, silvery flushed away and collected on wire trays. The
“Bean” or endosperm, yellow-green when dry cherries are dried in the sun for 5–10 d on wire
The site occupied by the “mucilage,” yellow-white, appears strapping, which ensures thorough air circula-
black in the figure, having been dissolved away during the
preparation of the sample.
tion, or on barbecues. Where the climate is not
A coffee cherry may contain one, two, or rarely, three seeds. sufficiently dry, sun drying can be partially or
completely replaced by artificial drying in tray
or tower air dryers for 24–36 h. Particular care
The dry process is applied where water is must be taken to avoid overheating the coffee,
scarce (as in Ethiopia and Brazil) and to the off- which results in the subsequent development of
grade cherries discarded during wet processing. a sour taste.
Freshly harvested cherries are spread out to dry
6 Coffee
Robusta cherries remain on the tree at maturity, Liberica cherries are rather pulpy and difficult
undergoing a slight fermentation, which is inter- to dry. Excelsa cherries ripen a few at a time and
rupted when the fruit dries. The ripe cherries are then drop to the soil when dry. In both cases,
selectively picked from the large shrubs by start- uncontrolled fermentation occurs, which con-
ing at the lower branches. The practice of strip- tributes to the unclean taste of their brew. Their
ping cherries off the tree at various degrees of importance is waning and they will not be dis-
maturity from green to dry, and drying, husking, cussed further.
and bagging them without sorting is becoming Coffee beans of various types are shown in
common. Figure 2.
and bleached beans are eliminated in Arabi- Grading by cupping is sometimes performed
cas; or fluorimetrically at 360 nm, to eliminate in the producing countries, but more often it is
“stinker” beans, which have an impure taste, in done in the consuming countries. Representa-
freshly cured washed Arabicas. The beans are tive samples of coffee are examined for defective
fed into chutes on counterrotating rollers or on beans and extraneous matter, roasted and noted
belts and viewed from different angles. The re- for uniformity of roasting, ground and brewed
jected beans are driven out by compressed air in porcelain equipment, tested by smelling, and
(capacity up to 150 kg/h per channel). tasted by swiftly drawing a spoonful against the
palate [14]. Washed Arabicas are graded accord-
ing to acidity, body, and flavor. Dry-processed
Brazilian coffees are subdivided in order of de-
creasing quality as strictly soft, soft, softish,
hardish, hard, and Rioy (a typical medicinal
off-taste). The best Robustas are neutral and
clean, while defective ones are harsh, rubbery,
or earthy.
Placing 60 or 69 kg of coffee (70 kg in
Colombia) into jute bags marked with the name
of the producer and the year of harvest readies
the coffee for shipping. The shipping of Robusta
coffee in bulk containers is now practiced.
Equipment used for separating impurities and
for grading green coffee beans is shown in Fig-
ure 3.
1) Provide an economic return that covers pro- 3) Secure social and working conditions in ac-
duction and living costs plus a further margin cordance with international standards, con-
for development, ducive to the maintenance of stable commu-
2) Treat the environment responsibly so that nat- nities.
ural resources remain available to future gen-
erations,
10 Coffee
Table 3. Decaffeination by low-moisture processes
Operation Medium Conditions
t, ◦ C Time, h
Soaking steam and water 22–100 0.25–5
Decaffeination water-saturated solvent
dichloromethane [22] 60–100 2–12
ethyl acetate [23] 80–105 3–12
fats and oils* [24, 25] 95–105 6–9
Deodorization steam 100–110 1–4
Drying heat 40–80 0.5–10
* No longer used.
4.3. Processes Using Supercritical Media and a scheme of decaffeination using supercrit-
ical carbon dioxide is shown in Figure 5.
Supercritical carbon dioxide, i.e., at tempera-
tures and pressures above the critical point of
31.06 ◦ C and 7.38 MPa (73.8 bar), can be used
to decaffeinate coffee by a process called de-
straction by its inventor K. Zosel. The super-
critical medium in a pressure vessel is circulated
through moist coffee, where it becomes charged
with caffeine. It is then passed through a sec-
ond pressurized vessel containing an adsorbing
medium such as activated carbon, resin, or wa-
ter, which retains the caffeine. The solubility of
caffeine increases with the density of supercrit-
ical carbon dioxide, thus increasing with pres-
sure at constant temperature and decreasing with
temperature increases at constant pressure. This
property can be used in the separation step. Be-
sides carbon dioxide, which is employed in at
least one industrial decaffeination plant, other
gases have also been proposed for decaffeina-
tion (Table 5).
Both carbon dioxide and propane can also
dissolve caffeine in the liquid state below the
critical temperature. The pressure – temperature Figure 4. Pressure – temperature diagram of carbon diox-
ide
diagram of carbon dioxide is given in Figure 4, Densities from 100 to 1200 g/L are given as the third di-
mension [36].
12 Coffee
Figure 6 shows the temperature profile, roast- up to 20 % more extractable matter when brewed
ing losses, moisture content, apparent bulk den- and a more pronounced taste. Less spectacu-
sity, and volatile aroma released by Arabica lar results can also be attained by using tradi-
beans with an initial moisture content of 10 %, tional conduction – convection roasters at high
heated up to 240 ◦ C in 12 min. Similar graphs final temperature conditions and low gas vol-
are obtained with roasting cycles of 6–18 min umes [44 – 46]. Figures 7, 8, and 9 show exam-
by adjusting the ratio of beans to hot air. With ples of batch and continuous industrial roasters
faster roasting cycles, down to less than 2 min, (courtesy of Probat, Emmerich, Germany).
uniformity of roasting throughout the bean is re-
duced. For a given roasting loss, the apparent
bulk density, quantity of aroma released, and
extractable solids at brewing increase. Robus-
tas need longer roasting times to attain the same
roasting losses as Arabicas. On subsequent cool-
ing, which may be accelerated by quenching the
roasted beans with water, residual carbon diox-
ide is still present in the beans and is slowly
released over a period of days.
Table 10. Carbohydrates in soluble extract from coffee roasted at three different degrees, wt % by monosaccharide constituents and by their
polymers (glycans) (determined as single sugars)
Components Light roast Medium roast Dark roast
Arabinose 2.4 1.7 1.5
Galactose 20.0 17.8 13.9
Glucose 0.9 10.4 0.7
Mannose 8.7 10.4 16.0
Total 32.6 31.2 32.7
Table 11. Carbohydrates in soluble extract from coffee roasted at three different degrees, wt % by degree of polymerization observed
(determined as total monosaccharide)
Components Light roast Medium roast Dark roast
Monosaccharides 3.34 2.89 2.01
Oligosaccharides 8.03 9.57 7.38
Polysaccharides 20.85 18.46 23.22
Table 17. Chlorogenic acids in roasted Arabica and Robusta beans, wt % [75]
Species State of the bean
Raw Light roast Medium roast Dark roast
Arabica 6.9 2.7 2.2 0.2
Robusta 8.8 3.5 2.1 0.2
roasting, and are present in roasted and instant 5.5, is less clear; for example, pH is lower for
coffees [76]. Table 18 shows the proportion of washed coffees than for natural coffees and for
chlorogenic acids in industrial extracts. extracts than for household infusions. The sour
A good correlation exists between titratable taste of infusions that have been kept warm for a
acidity of the roasted coffee infusion, to which period of time is due to an increase in free acids,
phosphoric acid (0.2–0.3 %) also contributes, in particular quinic acid formed by hydrolysis of
and the acid taste of the cup of coffee. The corre- esters, depsides, and lactones, as well as to loss
lation with pH, which may vary between 4.7 and of pyridine.
Coffee 21
Table 18. Chlorogenic acids in industrial extracts, wt % [77]
Component Quantity
n-Chlorogenic acid 0.7–1.9
Cryptochlorogenic acid 0.8–2.3
Neochlorogenic acid 1.0–3.5
Sum 2.5–7.7
3-Feruloylquinic acid 0.3–0.8
4-Feruloylquinic acid 0.3–0.8
5-Feruloylquinic acid 0.2–0.4
Sum 0.8–2.0
3,4-Dicaffeoylquinic acid traces – 0.5
3,5-Dicaffeoylquinic acid 0.1–0.3
4,5-Dicaffeoylquinic acid 0.1–0.4
Sum 0.2–1.2
proposed as a means to evaluate the relative pro- Values range from 8.7–12.2 % of the dry
portions of Arabica and Robusta beans in com- weight. No major differences exist between Ara-
mercial roasted coffee blends. bica and Robusta, although the higher concen-
Small quantities of the antioxidants, α-, β- tration of sulfur-containing amino acids in Ro-
and γ-tocopherols, and 5-hydroxytryptamides bustas may be responsible for the differences
have been identified. The content of tryptamides perceived in the volatile flavor between the two
in the wax increases with ripening of the cherry species. Table 20 shows amino acids and pro-
at the expense of tryptophan and decreases on teins in dried raw bean.
storage and also after various treatments of the
bean. For example, the reduced level in treated Table 20. Amino acids and proteins in dried raw beans
coffee (by steaming or by solvent washing) is Nitrogenous Proportion,Components
claimed to be an index of the reduction of ir- fraction wt %
Free amino 0.2–0.8 glutamic acid, aspartic acid,
ritating substances in coffee. Tryptamides are acids γ-aminobutyric acid, alanine, proline,
decomposed by roasting, and their relationship, and serine
if any, to health effects of the brew is at least are the most important; total
concentration is possibly lower in
doubtful [84]. Arabicas than in
Robustas
Proteins 7.9–12 composition near that of the free amino
acids, with 20 % of glutamic acid and
10 % each of both aspartic acid and
leucine; histidine, cysteine, and
methionine
are also present in small quantities
Soluble 25–40 % isoelectric point at pH 5.7 for Arabicas
proteins and pH 6.3 for Robustas
Structural 60–75 % linked with polysaccharides and
proteins chlorogenic acids in the cell wall
(15–25 %
soluble
in alkali)
The content of lipids in a cup of coffee de- Only traces of free amino acids remain after
pends on the brewing method and can reach a roasting since they are thermally unstable, un-
few percent in brews made from finely ground dergoing Maillard reactions with reducing sug-
Turkish coffee or when ground coffee is boiled ars, to release volatile components and form
with water; it is insignificant in filtered coffee in- melanoidins, a polymeric material. Up to 40 %
fusions (for physiological significance, see Sec- of the proteins are denaturated and decompose
tion 8.3). Small amounts of oil (between 0.2 and during roasting, although one-half of the ami-
1 %), obtained from roast and ground coffee or no acids present in the raw bean remain as ap-
from spent grounds, are often used as support for parent polypeptides with the exception of argi-
the reincorporation of volatile aroma into an in- nine, which is completely degraded. All avail-
stant coffee powder, but the amount of oil present able polypeptides are extracted at 30 % extrac-
in the cup is usually negligible. tion yield and constitute 10–12 % of an industrial
extract.
Part of the caffeine is present as the free crys- of preparation, and crop year. The mineral com-
talline base in the cytoplasm surrounding the cell position of dry coffee beans is shown in Table
vacuole. Part of it is in the cell wall, where it may 22.
be bound as a 1 : 1 π-complex with potassium
chlorogenates, although the complex may also Table 22. Minerals in raw beans
be an artifact formed during extraction [86]. Mineral Proportion,
Caffeine is thermally stable, and only a small wt %
Potassium 1.68–2.00
part of the quantity present in the raw bean Milds 1.68–1.70
is lost by sublimation during roasting. About Brazils 1.77–1.88
85 % passes into the domestic brew and almost Robustas 1.84–2.00
100 % into industrial extracts. The caffeine con- Calcium 0.07–0.35
Magnesium 0.16–0.31
tent of industrial extracts varies between 3.0 and Phosphate 0.13–0.22
5.1 % (dry basis) for spray-dried powders, which Sulfate 0.13
usually contain mainly Robustas, and between
2.5 and 3.7 % for milder freeze-dried products, Sodium and chloride content are low, un-
which contain more Arabicas. less the coffee has been contaminated by sea-
water during transport. About 90 % of the ash
is water-soluble and passes into the brew with
7.4.3. Other Bases only insignificant losses at roasting. The extrac-
tion yield of instant coffee can be estimated by
Trigonelline [535-83-1] (Arabica 0.6–1.2 %, the measure of the potassium content, when the
Robusta 0.3–0.9 %) and traces of betaine and blend and manufacturing losses are known [87].
choline are present in the raw bean. Some Trace metal concentrations in the bean, par-
trigonelline decomposes on roasting to nico- ticularly of manganese, may be correlated with
tinic acid (niacin or vitamin PP), pyridine, cup quality [88]
and other volatile aroma constituents. Residual
trigonelline is easily extracted and constitutes
0.9–1.7 % of industrial extracts. 7.6. Products Formed during Roasting
The principal thermally reactive constituents of
the raw bean are the monosaccharides and su-
crose, the chlorogenic acids, the free amino
acids, and trigonelline (Table 23). The termi-
nal arabinose and galactose units of polysac-
Traces of diamines, putrescine, spermine, and charides are split off, and the basic, sulfur-con-
spermidine have also been identified in the raw taining, and hydroxyamino acids are also eas-
bean. They are easily decomposed during roast- ily decomposed. The lipids are relatively stable,
ing. apart from an increase in free fatty acids due to
hydrolysis of the esters and some degradation of
linolenic acid and unsaponifiable matter. During
roasting, volatile aromatic compounds and poly-
7.5. Minerals
meric brown pigments are formed, while water
and carbon dioxide are liberated.
The ash content of green coffee averages 4.0 %,
Caramel, melanoidins, caffeine, and several
varying between 3.0 and 5.4 % (dry basis) due
aromatic constituents are responsible for the
to the botanical or geographical origin, method
characteristic bitter taste of roasted coffee.
24 Coffee
Table 23. Main reactive constituents lost during roasting
the green coffee volatile compounds, and those
Constituent Proportion in Fraction reacted, that together constitute the aroma of roasted cof-
raw bean, wt % wt %
Carbohydrates fee.
Monosaccharides 0.2–0.5 ≈100 A process [96] for improving the quality of
Sucrose 2–7 70–80 Robusta coffee proposes the removal of MIB by
Polysaccharides 20–25
Mannose 4–6 20–30
steam heating. Since roasting destroys MIB, the
Galactose 3 25–30 Robusta flavor character of a brew may be due
Glucose 1 5–10 to a combination of several factors [97].
Arabinose 1 50–60 The coffee aroma obtained by extracting ei-
Chlorogenic acids 4.7–8.6 40–98
Nitrogen compounds ther the roasted beans or the brew with selec-
Free amino acids 0.2–0.8 100 tive solvents, or by concentrating the headspace
Trigonelline 0.1–0.8 30–80 over the coffee or the brew, is separated in a gas
Lipids 1 3–5
chromatograph (GC). The smell of each peak is
evaluated by sniffing the exit port of the GC, and
7.6.1. Volatile Aroma [89] described by notes such as burnt, cocoalike, but-
tery, floral, etc. (Figure 12). Once the key aroma
Two hundred and thirty volatile compounds have components have been identified by mass spec-
been identified in the aroma of green coffee [90]. trometry, their relative impact can be measured
The majority of these compounds survives roast- by the aroma dilution technique, which consists
ing and may contribute to the roasted coffee fla- of injecting an increasingly diluted aroma into
vor [91]. Albeit made up of almost one thousand the GC until only a few peaks are still perceptible
volatile compounds so far identified (most of to the nose [98]. The most potent odorants iden-
them common to all roasted foods [92]), aroma tified in Arabica and Robusta brews are listed in
constitutes just 0.1% of the weight of roasted Table 25 [99].
ground coffee [93]. Various sensors, the so-called electronic
noses, have become available for the detection of
Table 24. Important off-flavors of (green) coffee odors: chemical families such as α-dicarbonyls,
Flavor Odor threshold Responsible chemical pyrazines, and sulfur compounds can be recog-
characteristics in water nized in the headspace when present at relatively
Medicinal, rioy 1.0 ng/L 2,4,6-trichloroanisole
(TCA) [93] *
high concentrations (at least 20 µg/L).
Earthy, robusta 2.5 ng/L 2-methylisoborneol The cup profile can be presented as a star di-
(MIB) [94] * agram (Figure 13) [100], in which each flavor
Musty 5.0 ng/L geosmin *
is semi-quantitatively described by a panel of
Fruity, rotten, stinker 5–10 mg/L ethyl esters of 2- and
3-methylbutanoic and expert tasters. Star diagrams are very useful for
cyclohexanoic acids comparing samples.
[95]
An important key aroma component is 3-
* Musty/earthy off-flavors in green coffee are usually due to
different combinations of these substances and of
mercapto-3-methylbutyl formate, which as a
methoxypyrazines; all these substances are probably of pure substance has a catty/blackcurrant smell,
microbiological origin and apparently occur more readily in but when added at very high dilution to a (sol-
dry-processed beans.
uble) coffee infusion it strongly enhances the
roasted coffee note [101].
In the 1990s the focus of coffee aroma re- The nature of this heterogeneous class of yellow-
search has switched from the mere identification brown to almost black pigments is still sub-
of more and more volatile substances to the se- ject to speculation. They are formed by two
lection of those that constitute the flavor of cof- main routes. In the first, small molecules in the
fee. Two types of aroma impact substances are cells, e.g., monosaccharides and sucrose, poly-
recognized: those that contribute to specific de- merize to yellow- to dark-brown caramel, which
fects (Table 24), usually already present among reacts with chlorogenic acids to form red- to
Coffee 25
Table 25. Concentrations of the most potent odorants in Arabica and Robusta brews
Odorant Arabica, µg/L Robusta, µg/L Odor threshold in
water, µg/L
2-Furfurylthiol 19.1 39.0 0.01
(E)-β-Damascenone 1.3 1.5 0.00075
3-Mercapto-3-methylbutyl formate 5.5 4.3 0.0035
3-Methylbutanal 550 925 0.35
Methylpropanal 800 1380 0.7
Methanethiol 210 600 0.2
5-Ethyl-4-hydroxy-2-methyl-3(2H)-furanone 840 670 1.15
2-Methylbutanal 650 1300 1.3
black-brown humic acids [103]. These sugars rial, composed of intact mannose units linked
also react with amino acids via the Maillard together by galactose, arabinose, free phenolic
reaction [104] to form yellow- to black-brown acids, free amino acids, and peptide side chains,
melanoidins [105]. can be identified. These form the matrix on
In the second, similar reactions may also oc- which the brown pigments are fixed. These pro-
cur in the cell wall following thermal degrada- cesses are depicted in the following diagram:
tion of the constituents. Partially soluble mate-
Figure 12. Odor profile of a dry ground roasted coffee. The vertical axis gives the mass spectrometric abundance. Known odor
thresholds are indicated on the peaks (courtesy Dr. H. Brévard)
8.2. Dietary Value tributes, via nicotinic acid, a fraction of the daily
nutritional requirement for niacin (vitamin B3
or PP), in amounts increasing with the degree of
Coffee is consumed at breakfast and between
roast, and might play a role in the diet of under-
meals as a stimulant, or after a meal as an aid
nourished people. The composition of a cup of
to digestion, particularly in Latin cultures. The
coffee is given in Table 28.
nutrient profile shows that a cup of coffee con-
Coffee 27
Figure 13. Sensory profile of a coffee brew. This sample has been characterized by a trained panel as possessing an intense
overall aroma and flavor, a roasted/burnt/smoked note, and an off-flavor described as stale/oxidized (courtesy Dr. S. Bobillot)
Table 26. Green coffee disappearance in the principal importing countries [thousands of 60-kg bags (source: International Coffee
Organization)] and consumption (kilograms per capita per year)
Country 2000 2001 2002 2003 2004 2005 Average kg per capita
Austria 862 1029 945 753 1032 809 905 6.64
Belgium 1304 987 1635 1719 1458 1307 1402 8.26
Cyprus 68 55 53 54 57 71 60 4.48
Czech Republic 550 655 646 556 546 534 581 3.27
Denmark 784 863 806 725 849 814 807 9.17
Estonia 99 110 112 118 133 150 120 5.42
Finland 896 941 962 988 1030 1048 978 11.21
France 5446 5474 5627 5360 4972 4952 5305 5.28
Germany 9283 9475 9064 9076 10177 8052 9188 6.90
Greece 713 578 864 1003 928 911 833 4.65
Hungary 733 740 709 611 561 394 625 3.83
Ireland 83 147 143 152 220 224 161 2.50
Italy 5164 5252 5180 5503 5405 5460 5327 5.57
Latvia 112 156 151 169 167 166 154 3.60
Lithuania 172 203 207 189 234 251 209 3.67
Malta 19 15 26 34 26 23 24 3.66
Netherlands 1911 1732 1641 1827 1599 1454 1694 6.26
Norway 658 711 692 682 710 743 699 9.17
Poland 1765 1958 1952 2096 2202 2133 2018 3.06
Portugal 723 750 743 745 771 749 747 4.54
Slovakia 262 308 298 318 319 368 312 3.28
Slovenia 177 193 201 182 195 200 191 5.66
Spain 3058 2869 2907 2826 2791 2959 2902 4.45
Sweden 1182 1259 1235 1181 1242 1162 1210 8.03
United Kingdom 2381 2261 2134 2196 2423 2174 2261 2.29
European Union 38 405 38 719 38 935 39 064 40 045 37 107 38 713 5.10
Switzerland 859 876 778 891 714 1056 862 7.06
USA 21 524 19 148 19 193 20 275 20 368 20 363 20 145 4.21
Japan 6876 6919 7209 6786 7167 7441 7066 3.32
Total (including 67 664 65 662 66 114 67 015 68 294 65 968 66 786 0.63
others)
28 Coffee
Table 28. Composition of a cup of coffee, mg
stimulates the central nervous system, shortens
Component Brew a Instant b reaction time, improves alertness, and reduces
Monosaccharides 0–150 15–150
Sucrose 0–200 0–200
mental fatigue [118].
Aliphatic acids 130–350 60–175 It is a cardiac muscle stimulant, a smooth
Chlorogenic acids 100–250 150–200 muscle relaxant, and improves physical perfor-
Lipids traces 0–10 mance [119], but not up to the point to be con-
Volatile aroma traces traces
Caffeine 50–150 50–100 sidered as a doping agent by the International
Trigonelline 30–60 15–35 Olympic Committee (2004). It does not induce
Minerals 100 200 addiction [121] and is neither mutagenic nor
Potassium 50 100
Niacin traces traces
teratogenic for humans at normal dosages and
Polymeric substances c consumption levels. At excessive levels, other
(by difference) 390–1230 920–1500 symptoms occur, such as stimulation of gastric
a
7.5 g of ground roasted coffee at 22 % extraction yield. acid secretion, increase of heart rate, sweating,
b
c
2.0 g of soluble coffee. nervousness, and sleeplessness. To date, the evi-
Polymeric substances contribute to the bitter taste in the cup
[110] and to the retention of aroma constituents in the soluble dence gathered from many studies “does not sug-
powder [111]; although measured as proteins and carbohydrates, gest that caffeine at present levels of consump-
they are unavailable as such after roasting. tion poses a hazard to public health” according to
the FDA, which has kept it on the GRAS (Gen-
erally Recognized As Safe) list [122].
8.3. Physiological Value
Other Physiologically Active Substances.
Coffee has been the subject of extensive sci- An association between coffee consumption and
entific research addressing its potential impact serum cholesterol levels, reported in 1983 [123],
on human health [112], caffeine attracting over is linked with the presence of cafestol (and kah-
90 % of it. weol) esters in the beverage [124]. The amount
present depends on the brewing technique (par-
Caffeine. Caffeine is the principal active in- ticularly on fineness of grinding, means of sep-
gredient in coffee, and its stimulating action is aration of grounds) and is independent of tech-
the basis for the popularity of coffee and the nological parameters (decaffeination, industrial
other caffeine-containing beverages [113, 114]. high-temperature extraction). The ester content
Indicative caffeine contents of coffee prod- is high for boiled, French press, and Middle
ucts are given in Table 29. Eastern coffees, where there is no separation
of grounds, intermediate in espresso coffee, and
negligible in instant and filtered coffees.
Table 29. Caffeine content of different coffee products
In recent years, more and more epidemi-
Product Portion, mL Caffeine, mg
Filter coffee 100–200 35–175
ologically evident positive effects on human
Filter decaffeinated 100–200 3–6 health deriving from a regular coffee consump-
Espresso coffee 20–60 30–75 tion have been highlighted [125]. Preventative
Instant coffee 100–200 35–115
effects against diabetes [126], hepatic cirrhosis
Instant decaffeinated 100–200 2–3
Ready-to-drink 225–285 60–200 and cancer [127], and Parkinson’s disease [128]
have been demonstrated by a large number of
Within minutes from ingestion, caffeine is studies. Regrettably, explanations for the under-
readily adsorbed and distributed through all of lying mechanisms are still missing. On the other
the tissues and organs, then it is degraded to sev- hand, a chemoprotective effect against colon
eral methylxanthines and excreted via the kid- cancer has been associated with the presence in
neys within a few hours [120], with only a minor coffee, beside cafestol and kahweol esters [129],
proportion appearing in the urine as unchanged of antioxidants [130] and enhancers of endoge-
caffeine. Metabolization time is longer in chil- nous detoxifying mechanisms [131].
dren and in pregnant women. The physiologi-
cal effects of caffeine at normal dose levels are Contaminants. Several types of contami-
well known [115], even if important individual nants are sometimes encountered in coffee. Pes-
variations exist [116, 117]. It is a diuretic that ticides [132], present in trace amounts in green
Coffee 29
coffee, disappear during storage, roasting and Quality, Elsevier Academic Press, Oxford
brewing. Paraffins used in the manufacture of 2005, pp. 87–178.
jute/sisal bags may contaminate the green beans 12. ISO 3509:2005 Coffee and coffee products –
[133]. Polycyclic hydrocarbons are formed dur- Vocabulary, International Organization for
ing roasting [134]; if allowed to accumulate in Standardization, Geneve 2005.
the cold spots of the roaster and of the cooler, 13. ISO 10470:2004 Green coffee – Defect
where they tend to co-sublime with caffeine, reference chart, International Organization for
forming whitish deposits, they may constitute Standardization, Geneve 2004.
14. ISO 6668:1991 Green coffee – Preparation of
a work health hazard. Ochratoxin A, a myco-
samples for use in sensory analysis,
toxin formed during cherry processing, storage
International Organization for Standardization,
or transport of the beans if moisture is uncon- Geneve 1991.
trolled, may be present in trace amounts in com- 15. Marketing and Processing of Coffee: Areas of
mercial coffee products [135], although the sub- International Cooperation, UNCTAD, Geneva
stance is partially destroyed during processing TD/B/C.1/PSC/31 1983.
[136]. Relatively high levels of ochratoxin A 16. ICO: Trade Statistics, International Coffee
may be present in instant coffees adulterated Organization, London, 2006,
with husks [137]. www.ico.org/trade statistics.asp (accessed
Acrylamide, a Maillard product derived by July 2006).
heating sugars with asparagine and present in 17. “Organic coffees”, World Coffee & Tea, 1994,
baked potato products and in bread crust [138], is Oct. 19.
formed in coffee during the early phases of roast- 18. Council Regulation (EEC) No 2091/91 of 24.
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[139]. Its carcinogenic effects, while observable L 198.1, 22.7.91; Council Regulation (EC) No
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Communities No L 186/1, 5.8.95.
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Colloids 1
Colloids
Gerhard Lagaly, Institut für Anorganische Chemie der Universität Kiel, Kiel, Germany