A WBC Injection Syringe) M Motherboard CPU

Main Controller
B HGB and WIC Lyse and Diluent Syringe M •1-1
Main Memory •1-1Waste
WasteChamber
Chamber11Pressure
Pressure
•1-2
•1-2Waste
WasteChamber
Chamber22Vacuum
Vacuum
C Sheath Syringe M
Datastation •1-3
•1-3HGB/WIC
HGB/WICSample
SampleInlet
Inletinto
intoHGB
HGBFlow
FlowCell
Cell
•1-4
•1-4Spare
Spare
D RBC Diluent Syringe M MPM •1-5
•1-5Detergent
DetergentInlet
Inlet into
intoHGB
HGBFlowFlowCell
Cell
M SDM1 •1-6
•1-6Waste
WasteChamber
Chamber11Drain
Drain
E Sample Probe Up.Down Soft
SoftKey
KeyInterface
Interface •1-7
•1-7WOC
WOCMixing
MixingChamber
Chamber
•1-8 Spare
•1-8 Spare
F Sample Aspiration Pump M Ticket
Ticket Printer
PrinterCom2
Com2
G WBC Sample Pump M Graphic
GraphicPrinter
PrinterLIS
LISCOM
COM11 •2-1
•2-1WOC
WOCSample
SampleInjection
Injection/Prime
/PrimeOutlet
Outlet
•2-2
•2-2WOC
WOCSample
SampleInjection/
Injection/Prime
PrimeInlet
Inlet
HSSL •2-3
HSSL •2-3WOC
WOCMixing
MixingChamber
ChamberRinse
Rinse
Video SDM2 •2-4 WOC Mixing Chamber Bubble
•2-4 WOC Mixing Chamber Bubble Mix Mix

SDM DATA BUS.

+5V, +/-12V, +24V (Unreg), MPM Video
•2-5
•2-5WOC
WOCFlow
FlowCell
CellOutlet
Outlet
+5V, +/-15V MAM, 68K, SPM, DCM •2-6
•2-6WOC
WOCFlow
FlowCell
CellDrain
Drain
•2-7
•2-7WOC
WOCFlow
FlowCell
CellSheath
SheathInlet
Inlet
•2-8
•2-8Spare
Spare

•3-1
•3-1RBC/PLT
RBC/PLTTransducer
TransducerLeft
Leftside
sideDiluent
DiluentRinse
RinseInlet

VACUUM/PRESSURE MODULE
PUMP Inlet
RELAY •3-2
•3-2RBC/PLT
RBC/PLTTransducer
TransducerRight
RightSide
SideDrain
Drain
•3-3 RBC/PLT Transducer Left Side Drain
•3-3 RBC/PLT Transducer Left Side Drain
SDM3 •3-4
•3-4RBC/PLT
RBC/PLTBubble
BubbleMix
PUMP

PUMP

Mix

VACUUM
ACCUM.
•3-5
•3-5RBC/PLT
RBC/PLTTransducer
TransducerRight
RightSide
SideDetergent
DetergentInlet
Inlet

WET
•3-6
•3-6 HGB Lyse PrimeNormally
HGB Lyse Prime NormallyClosed
Closed(NC)
(NC)

VPM
•3-7
•3-7RBC/PLT
RBC/PLTCount
Count
•3-8
•3-8RBC/PLT
RBC/PLTRBC/PLT
RBC/PLTMetering
MeteringTube
TubeVent
Vent
Vacuum Pressure VACUUM
ANALYZER
ACCUM. •4-1
•4-1HGB/WIC
HGB/WICDiluent
DiluentSyringe
SyringeInlet
WET 68K CPU •4-2
•4-2HGB/WIC
HGB/WICLyse
LyseSyringe
SyringeInlet
Inlet
Inlet
SDM4 •4-3
•4-3RBC/PLT
RBC/PLTDiluent
DiluentSyringe
SyringeInlet
Inlet
•4-4
•4-4HGB/WIC
HGB/WICLyse
LyseSyringe
SyringeDispense
Dispense
•4-5
•4-5WOC
WOCSheath
SheathSyrine
SyrineInlet
Inlet
+5V, +/-15V MAM, 68K, SPM, DCM
+5V, +/-12V, +24V (Unreg), MPM

•Vents 12PSI to ATMOS SOL 1 •4-6

•4-6Waste
WasteChamber
Chamber22Pressure
Pressure
•Regulates 9 PSI SOL 2
•4-7
•4-7Waste
WasteChamber
Chamber22Vacuum
Vacuum
•4-8
•4-8Waste
WasteChamber
Chamber22Drain
Drain
•Regulates 5 PSI SOL 3 HSSL
•Vacuum 1 Vent SOL 4
•5-1
•5-1Diluent
DiluentReservoir
ReservoirPressure
Pressure
•Regulates VAC 2 SOL 5
•5-2
•5-2Diluent
DiluentReservoir
ReservoirVacuum
Vacuum
DMA Control SDM5 •5-3
•5-3Sheath
SheathReservoir
ReservoirPressure
Pressure
•5-4
•5-4Sheath
SheathReservoir
ReservoirVacuum
Vacuum
•5-5
•5-5Detergent
DetergentReservoir
ReservoirPressure
Pressure
•5-6
•5-6Detergent
DetergentReservoir
ReservoirVacuum
Vacuum
DCM •5-7 Spare
•5-7 Spare
•5-8
•5-8Spare
Spare
12 Hg 4 PSI 9 PSI 12/8 Hg 12 Hg 4 PSI 12 PSI 12 Hg 9 PSI
RGT Res Dil Det Sheath WC1 WC2 Bubble Mix WC 1/2 Cap Piercer
Res Res ONLY •6-1
•6-1Diluent
DiluentOut
Out
MAM •6-2
•6-2Sheath
SheathOut
Out
Solenoid 98
SDM6 •6-3
•6-3 DetergentOut
Detergent Out
•8-1
•8-1WIC
WICTransducer
TransducerLeft
LeftSide
SideDiluent
DiluentInlet
Inlet
•8-2
•8-2WIC
WICTransducer
TransducerRight
RightSide
SideDrain
Drain
Voltage.

SPM •8-3 WIC Transducer Left Side Drain

HGBVoltage.

PMT1 PRE PMT2 PRE •8-3 WIC Transducer Left Side Drain
90 Degree 90D Depol SDM8 •8-4
•8-4WIC/HGB
WIC/HGBBubble
BubbleMix
Mix
0 Degree 10 Degree •8-5
•8-5WIC
WICTransducer
Transducer Right
RightSide
SideDetergent
DetergentInlet
Inlet
•8-6 Spare
HGB

•8-6 Spare
•8-7
•8-7WIC
WICCount
Count
WIC CDM 1
•8-8
•8-8WIC
WICMetering
MeteringTube
TubeVent
Vent
PMT1 PMT2
•9-1
•9-1Shear
ShearValve
ValveBack
BackFlush
Flush
•9-2
•9-2Shear
ShearValve
ValveDrain
Drain
RBC/PLT
RBC/PLT RBC/PLT •9-3
•9-3 CS or SL WasteDrain
CS or SL Waste Drain
Trans
Trans PRE AMP
FCM SDM9 •9-4
WIC
WIC WIC •9-4Open
OpenSample
SampleAspiration
AspirationProbe
ProbeWash
WashBlock
BlockDrain
Drain
Trans •9-5
•9-5Open
OpenMode
ModeSample
SampleAspiration
AspirationInlet
Inlet
Trans PRE AMP
•9-6 CS or SL Sample Mode Inlet
•9-6 CS or SL Sample Mode Inlet
CDM 2 •9-7
•9-7Open
OpenMode
ModeWash
WashBlock
BlockRinse
Rinse
Switching •9-8
Power Supply CCW
CCW •9-8SL
SLWash
WashBlock
BlockRinse/
Rinse/CS
CSVacuum/Pressure
Vacuum/Pressurefor
for
Limit Needle
NeedleMovement
Movement
Power Ext
ExtWaste
WasteSensor
Sensor Shear Valve LimitSwitch
Switch
Distr Module +5V, +/-12V, FCM, VPM RBC/PLT WIC
Driver Diluent
120 V AC

Dil Metering Metering CW Diluent

DilRes
ResSensor
Sensor CW
Module Module Limit
LimitSwitch
Switch Reagent Sheath
Sheath Sheath
SheathRes
ResSensor
Sensor Empty
+12V (Unreg.), +24V (Unreg.) CDM2 Sensor Detergent
Detergent
Det Start
StartSwitch
Main
MainPower
PowerSupply
Supply DetRes
ResSensor
Sensor Switch
HGB HGB/WIC
HGB/WICLyse
Lyse
Probe
ProbeSwitch
Switch
LASER Flow Cell
Sample
SampleSwitch
Switch
POWER LASER
+/- 5V
SUPPLY
+100V
+12V (Unreg.), +24V (Unreg.) CDM1
Document Control Number 203271-102 ©2007, Abbott Laboratories, Abbott Park, Illinois
 X-B Hints
Lymphocyte and Neutrophil 00
and 100 Neutrophil 9000 and
Neutrophil 90 and 90
9000 D
D MCV MCV M C V = D ire c t M e a s u re m e n t
MCV MCV
Whole Blood Positioning Whole Blood Positioning
Whole Blood Positioning
255 255 Hgb
RBC
900 D MCH MCV HGB x 10
00 G BOOT SYSTEM ON SET UP DISK
S R MCH Hgb RBC
CD3000 GET STORES VALUES ON FLOPPY
A Function Keys RBC
I CD3000 PUT RECALLS VALUES FROM FLOPPY
N CD3500/3700/3200 SAVE STORES VALUES ON FLOPPY MCV
Z Main Menu F12 F8 = DOS Prom pt
U CD3500/3700/3200 RESTORE RECALLS VALUES FROM FLOPPY
E L Calibration F12 F1 = Dil Factors
SERVICE DISK HAS DOS FILES ON IT Hgb
A LASER OUTPUT >5MV <7MV WBC (WOC) VOLTAGE READING SCREEN Set Up F12-F1 = Color Selection RBC HGB X100
RI FD SLIT APPOX 1.2 - 1/5 MV OFFSETS VOLTAGES 1-4 <2.0 VOLTS
Diagnostics MCHC MCV HCT
T V a c A c c um le a d s B ro w n To p CHANNEL 1-4 GAIN SETTINGS <2500
F12 F1 = Set Point Entry MCHC Hgb
Y R e d B o t t o m C he c k f o r Gro und Ele c t N o is e DYNODE VOLTAGES <900 VOLTS
F12 F2 = Latex Blood Com p RBC HGB X 1000
MCV MCV X RBC
1. Blood/Latex Sam ple
VACUUM EQUATION Possible Cause
2. Cell Regions
0 255 0 255 DESIRED LEVEL
MCHC MCH Sam pling Valve Misfunction
F12 F3 RBC Edit Factor Entry Hem oglobin High bias
100 - COMPLEXITY 900 LOBULARITY VAC D/A SET = 30 X 4095 F12 F4 Peek/Poke Com m and Rbc Low Bias
If the WBC gains are set correctly, the center of the If the whole blood gains are set correctly, the center of the MCHC MCH
1. Motor Pow er Setting Hem oglobin Low Bias
Lymphocyte population should be positioned at one and one Neutrophil population should be positioned similar to the
hash mark on the 0° and 10 ° and the Neutrophil population above graphic. 2. Motor Moving Check Rbc High Bias
should be at 2.5 and 2.5 hash marks.
PRESSURE EQUATION MCV MCHC MCV Bias
3. Aperature Current
DESIRED LEVEL MCV MCH Protein Build up RBC Apert
F12 F5 = Raw Data Off F12 F6 On
Neutrophil = Monocyte = Basophil = PRES D/A SET = 30 X 4095 MCV MCHC Electronic Malfunction
Eosinophil = Neutrophil = F12 F7 = Debug Info Off F12 F8 On
Lymphocyte = Eosinophil = There are many Cause for X-B changes but think of the
WIC QC Lim its Hidden Setup QC QC Lim its F12 F1 Parameters involved in each one.....

WHOLE BLOOD GAIN VERIFICATION/ADJUSTMENT OPTICAL CV VERIFICATION

QUICK VOLTAGE CHECK
Method Detector Target Channel Range •-Pipette 2.0 ml Micro Particle solution into an
VACUUM RECOVERY SRP empty Vacutainer.
•-Mix a bottle of 7.0 λM Beads.
<3.5 AT SEA LEVEL OD 160 3 Preparation •Add 15 drops of the 7.0 λM Beads to the
PSM Gran Vacutainer and invert ten times to mix. .
TP1 +25 to +36 VDC
10D 149 3
5.0 is the max value that will be shown on the test. Target Select Run Open Tube
TP2 +13 to +18 VDC Channels 90 D 125 3
Diagnostic> More two times
TP3 +14.6 to +15.4 VDC 90 D DEP 21 2 Select >Gain Adjust>Verify Gain WOC
TP4 Analog Ground 7.0 WOC OD 35 2 RUN AND VERIFY CV RANGE
PRESSURE RECOVERY TP5 -14.6 to –15.4 VDC Detector Target CV Typical CV
7.0 WOC 10 D 65 2
<2.5 AT SEA LEVEL
TP6 100 Volt Return S 7.0 WOC 90 D SHEET 6 00 < 6% 4.0 – 4.5%
5.0 is the max value that will be shown on the test. TP7 +94 to +106 VDC R 100 < 5% 3.0 – 3.5%
P 7.0 WOC 90 D DEP SHEET 10
5.0 WIC Impedance WIC 131 1 900 < 18% 10.0-12.5%
Motor Power Check
5.0 RBC Impedance RBC 98 1 900D < 18% 10.0-12.5%
COUNT TIMES Low 1.6-2.4 Med 3.44 –5.16 High 4.88 – 7.32
3.3 PLT Impedance PLT 136 1
Refer To ISA CD3P-10 For Optical Bench Troubleshooting.
WIC UP 1.5 +/-0.35
WIC COUNT 4.5 +/- 0.3
Characteristic affecting WBC Results CD 35/3700 OPERATIONAL VERIFICATION SYSTEM SPECIFICATION
RBC UP 3.5 +/-0.35 0 0 0 0
WOC WIC RBC HGB MCV PLT RDW MPV
0 10 90 90 D
Parameter Target Range
RBC COUNT 6.5 +/- 0/3 Background 0.30 K / L 0.30 K / L 0.03 M/ λL 0.20 g / dL 10.00 K / λL
WBC X X PRESSURE 1 12 psi +/-0.8
NRBC X X X
PRESSURE 2 9 psi +/-0.5
Neu-Eos Open M. Precision
SYRINGE ASSEMBLY Thresholding X X <2.5% < 2.8% <1.5% <1.2% <1.0% <5.0%
PRESSURE 3 5 psi +/-0.3
Open M.Carryover [(Background(1)– Background(3) ) / (Last
<3.0 whole blood of precision - Background(3))] X 100 > 1%
LEF T TO R IGHT 3500/3700 RstRBC
Flagging X X X <3.0
1 WOC SAMPLE
Closed M. Precision <2.5% < 2.8% <1.5% <1.2% <1.0% <5.0% VACUUM 1 12 Hg +/-0.5
2 WOC SHEATH VarLym
Flagging X X X
3 HGB LYSE Closed M.Carryover [(Background(1)– Background(3)) / (Last whole blood of precision - Background(3))] X 100 > 1% VACUUM 2 IDLE 12 Hg +/-0.5
4 WIC DILUENT Band Flagging X X
Open M. Vs Closed M. VACCUUM 2
5 RBC DILUENT <1.75% <1.75% <1.25% <1.25% <1.25% <3.5% 8.5 Hg +/-0.5
IG Flagging X X DURNING COUNT

Blast Flagging X X
OPEN WOC TO WIC <3.0%
X X HGB VOLT 5.0 +/-0.2
CLOSED WOC TO WIC <3.0%