NEO HERBAL

VASAI ( EAST ) MAHARASHTRA

STANDARD OPERATING PROCEDURE

TITLE : S.O.P OF REFRACTOMETER

S.O.P NO : NH/QC/SOP/03/2017
Effective date : 12/07/ 2017
Review date : 12/07/2019
Responsibility : Quality Control Department
Model no : RSR-1 / S.NO – 2883
MAKE : Rajdhani scientific instrument co.
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OPERATION AND CALIBRATION OF REFRACTOMETER

A . OPERATING PROCEDURE .
1. Ensure that the instrument is free from dust .
2. Ensure that all the parts including eyelens , prisms and mirrors are clean before
operation
3. Place the instrument on the table where adequate light can be reflected through the
mirror .
4. Open the prism lock and place few drops of liquid testing sample at the temperature
specified.
5. Allow the liquid to smear on the lower prism surface quickly close the prism lock.
6. Adjust the eyepiece cross wire with aknob by rotating it in a clockwise or anticlockwise
direction so that the sharp demarcationline passes through its centre .
7. Sharp demarcation is produced in the form of acircle having half light & half dark
portion.
8. Fine adjustment can be done by knob provided just above the prism .
9. Note down the reading from the scale with the help of the magnified lens of the
instrument .
10. Use thermometer and water jacket with the tubes provided in the instrument whenever
it is required .
11. Clean the instrument and keep under cover when not in use .
B. CALIBRATION PROCEDURE .
To achieve accuracy , the instrument should be calibrated against distilled water , which
has a refractive index of 1.3325 at 25 C AGAINST the refrence liquid given below :
REFERENCE LIQUID STANDARD R.I. OBSERVED R.I TOLERANCE
AT 20 C
Carbon tetrachloride 1.4603 0.0001
Toluene 1.4969 0.0001
O- 1.6176 0.0001
methylnapthalene

C. GENERAL CARE AND PRECAUTION .

. Proper handling of the instrument .
.temperature should be carefully adjusted and maintained since refractive index varies
significantly with temperature .

PREPARED BY APPROVED BY QC/IC

REENA SINGH PINAKIN SHAH