Sheet1

Methylene Blue Standard Curve

Calibration data
Concentration Instrument

Absorbance
of standards readings
1.6000
0.2500 0.2900
0.2000 0.5840
0.1000 1.1760 1.4000 f(x) = - 5.9040x + 1.7656
0.0500 1.4700 R² = 1.0000
1.2000

1.0000

0.8000

0.6000

0.4000

0.2000

0.0000
0.0000 0.0500 0.1000 0.1500
Methylene Blue0.2000 0.2500
Value (g/100g) 0.3000
R e s id u a ls

0.10%
0.05%

0.00%
0.0000 0.0500 0.1000 0.1500 0.2000 0.2500 0.3000
-0.05%

-0.10%

Standard deviation of the residuals =

value Error (σ) % Error

Slope -5.9040 0.0057 -0.10%
Intercept 1.7656 0.0010 0.05%

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Application to unknowns
Readings of Calculated Calculated % Calculated
the unknowns concentration error (σ) error
0.4400 0.2245 0.000 0.12%
0.6400 0.1907 0.000 0.13%
56

Blue0.2000 0.2500
Value (g/100g) 0.3000

0.2000 0.2500 0.3000

0.0%

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Residual calculations
Concentratio
ns of the Predicted Actual
standards readings reading
0.2500 0.2896 0.2900
X*Y X*X (Y-Ybar)^2 (Y-Ypred)^2 0.2000 0.5848 0.5840
0.0725 0.0625 0.348 0.0000 0.1000 1.1752 1.1760
0.1168 0.04 0.088 0.0000 0.0500 1.4704 1.4700
0.1176 0.01 0.088 0.0000
0.0735 0.0025 0.348 0.0000
0 0 0.000 0.0000
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0 0 0.000 0.0000
0 0 0.000 0.0000
0 0 0.000 0.0000
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0 0 0.000 0.0000
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0 0 0.000 0.0000
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0 0 0.000 0.0000

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Residuals (%
Actual- of max
predicted reading)
-0.0004 0.03% ΣX ΣY Σ(X*Y)
0.0008 -0.05% sum (Σ) 0.6000 3.5200 0.380
-0.0008 0.05% number of poin 4.0000
0.0004 -0.03% average (mea 0.1500 0.8800 0.0951

slope -5.9040
intercept 1.7656

Coefficent of determiniation, r2 1.0000

Summary of equations:
n=number of x,y data points
sumx=Σx
sumy=Σy
sumxy=Σx*y
sumx2=Σx*x
meanx=sumx/n
meany=sumy/n
slope=(n*sumxy - sumx*sumy) / (n*sumx2 - sumx*sumx)
intercept=meany-(slope*meanx)
ssy=Σ(Y-meany)^2
ssr=Σ(Y-intercept-slope*X)^2
R2 =1-(ssr/ssy)
sdb=standard deviation of intercept=SQRT(ssr/(n-2))*SQRT(sumx2/(n*sumx2 – su
sdm=standard deviation of slope=SQRT(ssr/(n-2))*SQRT(n/(n*sumx2 – sumx*sum

Note: The if( ) functions in some of the cells

are not part of the actual numerical calculation.
Their purpose is simply to blank out or zero
out cells that are not being used at the monent.

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Σ(X*X) Σ(Y-Ybar)^2 Σ(Y-Ypred)^2

0.115 0.87 0.0000
sdb sdm
0.001 0.006

Standard deviation of the residuals =

ssr/(n-2))*SQRT(sumx2/(n*sumx2 – sumx*sumx))
(n-2))*SQRT(n/(n*sumx2 – sumx*sumx))

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