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Service Manual
Information contained in this manual is copyrighted by Bio-Rad Laboratories, Inc. None of the contents may be
reproduced in full or in part by any person without the prior written approval of Bio-Rad Laboratories, Inc. This
manual is intended to provide information to assist the qualified field or instrument service technician to repair,
maintain, and order replace parts for the instrument supplied. Every reasonable effort has been made to keep
the information in this manual current and accurate as of the date of publication or revision. However, no guar-
antee is given or implied that the manual is error-free or accurate about any specification.
100-2414E 02/06 i
About this manual This document is part of Life Science Group’s commitment to provide
you, the service professional, with adequate information to effectively
support Bio-Rad instruments. Additional copies of this service document
may be ordered using part number 100-2414.
ii 100-2414E 02/06
Revision Control List
Each page of this manual has the catalog number, revision level and revision date for that page. Subsequent
revisions to a page will be indicated by changes to the revision level and revision date for that page. A sum-
mary of changes for all revisions is listed in the following table.
Page Changed
Revision Description Date
Number By
A - Initial Release A. Gandy Feb. 2002
B 4-11 to 4-12 Updated Checkmark Plate procedure A. Gandy June 2002
5-1 to 5-4 Added optics cleaning procedure
6-1 to 6-25 Added part numbers to sections; Updated Figure 6-9
6-16 Added check of plate position
7-1 to 7-2 Added Checkmark Plate; Updated Figure 7-3
7-4 Added Section “AdjustingPlate Carrier Belt Tension”
7-6 Added Section “Alignment of Plate Carrier”
8-5 Added Table 8-4 “Checkmark Plate Failures”
C Sec 1 thru 10 Manual Updated to Add Model 680XR A. Gandy Mar 2004
5-1 Added Note
6-3 Added 6.3.3 Model 680 XR Lamp
6-12 Added Table 6-1
6-15 to 6-16 Added 6.3.11 Model 680 XR Lamp Control PCB
6-22 Added 6.3.17 Model 680 XR Light Balancing Filter
7-8 Added Table 2 Firmware History
8-1 Updated Table 8-1
9-3 to 9-11 Updated parts information
App D Added Appendix D
D 6-12 Added Backlit LCD to Table 6-1 A. Gandy Sept 2005
7-6 Updated to reference Section 7.7
7-9 Updated Firmware Table 7-2 and added 7.6.3
7-10 to 7-11 Added Section 7.7 QC Software
9-5 to 9-11 Updated parts
E 7-8 to 7-9 Updated Firmware version 1.71; Table 7-2 update A. Gandy Feb 2006
7-11 to 7-15 Updated 7.6.4 Firmware Downloading
7-17 Added Section 7-8 External Printer Setup
8-1 Updated troubleshooting printer
9-11 Added Shield Cover
Section 1 Introduction
1.1 General Inforation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.2 Service Philosophy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.3 Safety and Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.4 Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Section 3 Installation
3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.2 Visual check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.3 Installation Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.4 Install Paper Roll (Optional Internal Printer) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.5 Computer Interface (Optional). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Section 8 Troubleshooting
8.1 About this Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1
8.2 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1
Table 8-1 General Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1
Table 8-2 Error Codes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-2
Table 8-3 Firmware Downloading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-4
Table of Contents
Section 10 Drawings
10.1 About this Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-1
10.2 Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-1
Block Diagram
Wiring Diagram
Appendix
Appendix A Model 680 System Checkout Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-1
Appendix B Model 680 XR System Checkout Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-1
Appendix C Checkmark Check Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-1
Appendix D Error Flow Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D-1
Introduction
Section 1
Introduction
Bio-Rad is not responsible for any injury or damage caused by the use of this instrument for purposes other
than for which it is intended or by modifications of the instrument not performed by Bio-Rad or an authorized
agent.
1.4 Conventions
The following conventions are used throughout this manual.:
• Left and right sides of the unit are as viewed from the front, operator’s position, unless otherwise stated.
• Dimensions are in millimeters, unless otherwise stated.
Section 2
System Overview
2.1 Introduction
The Model 680 and Model 680 XR Microplate Readers are an eight-channel, vertical pathlength photometer
that measures absorbance of the contents of 96 well mictotitration plates. They can perform single or dual
wavelength measurements, and can report absorbance values to three decimal places.
The Model 680 and 680 XR Microplate Readers can be programed by entering commands through the keypad
to define plate reading conditions and data analysis conditions as a protocol data, and select report types. A
printed report is produced by an optional on-board thermal printer or external printer. An optional incubator is
also available.
The Model 680 and 680 XR Microplate Readers feature a built-in RS-232C serial interface device for conve-
nient computer interfacing. The Microplate Manager software for personal computers offers a complete analy-
sis program. Microplate Manager supports Windows 95, Windows NT and Windows 2000.
Microplate Manager Version 5.2.1 added support for the Model 680XR reader.
The following items are included with the Model 680/680 XR Microplate Reader system:
• Microplate Reader with 415 nm, 450 nm, 490 nm and 655 nm interference filters installed
• Power Cord
• Spare Fuse
• Dust Cover
• Instruction Manual
• Warranty Card
This instrument is designed and certified to meet EN61010 requirements which are internationally accepted
electrical safety standards. Certified products are safe to use when operated in accordance with the Instruction
Manual. This instrument should not be modified or altered in any way. Alteration of this instrument will result in
voiding of the manufacturers warranty, void regulatory certification and create a potential safety hazard.
This equipment has been tested and found to comply with the limits for Class A digital device, pursuant to Part
15 of FCC rules. These limits are designed to provide a reasonable protection against harmful interference
when the equipment is operated in a commercial environment. This equipment generates, and can radiate
radio frequency energy and if not installed and used in accordance with the Instruction Manual, may cause
harmful interference to radio communications. Operation of equipment in a residential area is likely to cause
harmful interference in which case the user will be required to correct the interference at their expense.
2.5.1 Calculations
The Model 680 and 680 XR Microplate Readers uses Beer’s Law to calculate the absorbance value of each
well. Beer’s Law states that absorbance is equal to the Log10 of the ratio on the baseline measurement
intensity (Io) to the Sample measurement intensity (I).
Before measuring the plate, the reader takes a reading for all eight photodiode channels. These values are
recorded as the baseline measurement (Io) values for each channel, respectively. The reader then records
the sample measurement (I) value for each well, and calculates the absorbance using these values. Chan-
nel-to-channel error is significantly reduced because the Io value for a given channel is used only in deter-
mining the absorbance of the wells of that channel.
2.5.2 Limits
The Model 680 and 680 XR Microplate Readers display absorbance readings with absolute values up to
3.500. Absolute values greater than 3.500 are “out of range” and are displayed as either “*.***” or “-*.***”.
The first time the instrument is turned on, or after a battery replacement, the following default information will
hold in memory:
1. Both Administrator and Common user security password are set to “00000”
2. Laboratory name is “Bio-Rad Laboratories”
3. All wavelength values of the filters are set to “---” nm
4. The date and time of the calendar chip are set to 1 day, 1 month, 00 year, 0 hour and 0 minutes
5. None of the plate reading data is available
6. End Point protocol is chosen and the protocol number is set to #1
2.5.4 Reports
Eight types of reports for End Point protocol can be generated by the Model 680 Microplate Reader:
1. Raw Data Report
2. Absorbance report
3. Limit Report
4. Matrix Report
5. Cut Off report
6. Curve Fit Report
7. Concentration Report
8. Difference report
The reader will remain in the remote control mode until it receives “RL” command from the host computer or
until the “Start/Stop” key is pressed.
Transmission: Baud Rate 9600, Data Width 8, Stop Width 1, Parity None
Signal Assignment:
2.6.1 Features
• Operating Panel: Membrane pad with 4 arrow keys, 7 function keys and 11 numeral keys
• Display: LCD, 4 Lines x 20 Alphabet Characters
• Computer Interface: Bi-directional RS-232C serial communication port, 9 pin D-sub receptacle
• External Printer Port (only when internal printer option is not installed): Centronics interface with ASCII
character code; 25 pin D-sub receptacle; 80 characters output per line
• Report Types: Raw, absorbance, matrix, limit, cutoff, concentration, curve fit, difference, kinetic
• Applicable Plates: Standard 96 well microtitre plate of polystyrene - flat, “U”, “V” rigid bottom
• Mixing: 3 speeds (low, medium, high); Programmable time period 0-999 seconds
• Warm-up Time: 3 minutes
2.6.2 Optical
• Photometric Methods: Single and dual wavelength
• Photo detectors: 8 silicon diodes for measurement and 1 silicon photodiode for reference
• Light Source: Tungsten halogen lamp, 20 Watts with 3000 hours average lifetime
• Model 680 Spectral Range: 400 nm to 750 nm
• Model 680 XR Spectral Range: 340 nm to 800 nm
• Interference Filters: Wavelength Tolerance +/- 3 nm; Band Width: 10 nm typical
• Filter Capacity: 8 filters
• Model 680 Standard Filters: 415, 450, 490, 655 nm
• Model 680 XR Standard Filters: 340, 405, 415, 655 nm
• Optional Filters: 340 through 800 nm (in 5 nm increments)
• Fast Reading Speed: 6 seconds with single wavelength reading; 10 seconds with dual wavelength
reading
• Standard Reading Speed: 15 seconds with single wavelength reading; 30 seconds with dual wave-
length reading
• Indication Range: 0.000 to 3.5 OD
2.6.3 Performance
• Resolution: 0.001 OD
• Accuracy: +/- 1.0% (or 0.010 from 0 to 3.0 OD at 490 nm)
• Linearity: +/- 1.0% (from 0 to 2.0 OD); +/- 2.0% (from 2.0 to 3.0 OD)
• Reproducibility: 1.0% (or 0.005 from 0 to 2.0 OD); 1.5% (from 2.0 to 3.0 OD)
• Inter-Channel Variation: 1.5% (or 0.005 from 0 to 3.0 OD)
• Stability and Drift: 0.010 OD at OD=1 with single wavelength reading
2.6.4 Thermal
• Incubator (Optional): Temperature Set Point: 25oC to 45oC (at least 5oC above ambient)
Set Point Accuracy: +/- 0.5oC (set point = 37oC and room temperature = 25oC)
Well-to-Well Uniformity: 0.5oC (set point = 37oC and room temperature = 25oC)
Warm-up Time: 5 minutes (set point = 37oC and room temperature = 25oC)
2.6.5 Electrical
• Line Voltage: Universal Input: 100-120 VAC, 200-240 VAC, 50/60 Hz
• Fuse: T3.15 Amp, 250 Volts, 20mm x 5mm
• Power Consumption: 100 VA maximum
• Battery: Lithium, CR2032, 3 Volts; average lifetime 5 years
2.6.6 Physical
• Dimensions: 340 x 330 x 152 mm
• Weight: 5.5 kg
Section 3
Installation
3.1 Introduction
The Model 680 and 680 XR Microplate Readers are easy to install. Prior to installation, verify that all system
components have been received.
4. While pressing the Paper Feed key, direct the pointed end of the paper into the paper feed slot in the
underside of the printer until the printer grabs the paper and feeds it through the slot in the top of the
instrument.
Note: Microplate Manager version 5.2.1 is required for Model 680XR support.
Section 4
System Checkout
4.1 Introduction
This section provides the system checkout procedure for the Model 680 and 680 XR Microplate Reader. Suc-
cessful completion of the tests in this section represent the Operational Qualification of the instrument. A Sys-
tem Checkout Report is available in Appendix A and B.
The system checkout procedures for the Model 680 and 680 XR Microplate Readers require the use of a
remote computer using Microplate Manager software.
The Model 680 and 680 XR Microplate Reader contains an on board checkout procedure, Checkmark Valida-
tion Program, which requires the use of a special Checkmark test plate.
4.4.1 General
1. All system checkout procedures in this section will be performed with Microplate Manager via PC
computer.
2. The Checkmark Validation Program, if available, may be used for system checkout as an alternate
method (paragraph 4.5).
4.4.2 Setup
1. Setup computer.
2. Install Microplate Manager.
3. Install HPK protection key.
4. Connect RS-232 cable from computer to reader.
4.4.4 Startup
1. Turn ON the reader power switch. The LCD will display the version number of the on board firmware.
Record Firmware Version.
2. After about three seconds, the instrument will perform a self test diagnosis that takes about ten sec-
onds.
3. When prompted by the Log In screen (Administrator), enter the password “00000”. Press Enter.
4. The instrument will be placed in remote mode. “Remote Mode” will appear on display and touch keys
will become nonfunctional.
4.4.9 Accuracy
1. This test is performed with the use of the yellow dye test plate.
2. Use the formatted plate template from the Air Read above.
3. Click on “Reports”.
4. Check the “Raw Data Report” and “Unknown Concentrations”. Click “OK”. See Figure 5.
5. Verify that the actual well temperature is 37 ° C +/- 0.5 ° C for each well (A1, A6, A12, D6, D9, H1,
H6, and H12).
4.5.1 General
1. The Checkmark program is used with the Checkmark Reader Performance Check Plate for validat-
ing the performance of the Model 680 Microplate Reader. It will test the following parameters:
Default Measurements
• Accuracy
• Precision
• Linearity
Optional Measurements
• Plate Positioning
• Cross-talk
• Filter Check
• 0 OD and Overflow
• IR Spectral Blocking
2. The Checkmark program resides in the Model 680 Microplate Reader programs.
4.5.2 Procedure
Entering Checkmark Plate Reference Data
1. Turn on Model 680 Reader and enter password.
2. Press “Memory Recall” button.
3. Select “Protocol” and press “Enter”.
4. Select “Checkmark” and press “Enter”. Press any key if instructed.
5. Press the “Edit” button.
6. Select “Protocol” and press “Enter”.
Section 5
Preventative Maintenance
5.1 Introduction
This section provides the preventative maintenance procedure for the Model 680 and 680 XR Microplate
Reader. Preventative maintenance is recommended at least once a year.
5.3.1 Firmware
1. Connect the power cord to the connection on the back if the instrument.
2. Turn ON the power switch on the rear panel. The LCD will display the version number of the firm-
ware.
3. Record firmware version.
4. Turn OFF instrument.
5. Update to latest firmware if applicable (see Section 7.6).
3. Visually inspect for cleanliness of lenses. Clean as required with lens tissue and isopropyl alcohol
(see Section 5.3.3).
4. Reassemble instrument.
8. Clean lenses as required. If removal of bottom lenses is required for cleaning, remove plate carrier
and analog board (see Section 6.3.12).
9. Remove bottom lens block (Figure 5-4).
Section 6
Removal and Replacement
6.1 Introduction
This section provides the parts removal and replacement procedures for the Model 680 and 680 XR Microplate
Reader.
3. Remove the screw which holds the metal cover in place (Figure 6-2) and remove cover.
Note: Surface of Cover may be Hot! Allow to cool before replacing lamp.
2. Remove the two screws (arrows) that hold the black plastic cover in place (Figure 6-4) and remove
cover assembly.
Note: Do not tough new lamp with fingers. Oil on lamp may reduce lamp life.
2. Remove the top cover to left side of the instrument (Figure 6-7).
3. Remove three screws securing printer assembly and remove assembly (Figure 6-9).
4. Prior to installing printer assembly, set the jumpers on the printer control board as follows:
3. Remove three screws and lift out keypad board (Figure 6-13).
Note: Check the model and serial number for the correct replacement board.
4. Lift the plate carrier assembly, disconnect the cables and remove assembly (Figure 6-20).
5. Remove two screws securing the cover over the analog board (Figure 6-21).
7. Remove four screws securing analog board and remove (Figure 6-23).
8. Install new plate carrier assembly or analog board in reverse order of the above steps.
Note: Check the model and serial number for the correct replacement board.
Instrument
Jumper Jumper Placement
Configuration
Without Incubator JP2 Jump
With Incubator JP2 No Jumper (Open)
5. Install new incubator control board in reverse order of the above steps.
6. Perform incubator electronics adjustment as follows. Disconnect cable from plate carrier to CN52.
7. Connect cable into Incubation Adjustment Tool, 9310183, 107.79 ohm connector on tool.
8. Turn on instrument.
9. Measure voltage between TP5 and TP0 (see Figure 6-26).
10. Adjust trim pot VR1 until the voltage measures 0.000V +/- 0.001V.
11. Connect cable into Incubation Adjustment Tool, 116.47 ohm connector on tool.
12. Measure voltage between TP5 and TP0.
13. Adjust trim pot VR1 until the voltage measures 5.528V +/- 0.006V.
14. If voltage is out of range, repeat steps 3 through 9.
Ground Screw
3. For ease of disassembly, turn over assembly. To access lamp control board, remove the five screws
securing the lamp PCB cover (Figure 6-28).
4. Remove cover.
Note: Heat sink will be removed as a result of removing the two side screws.
5. Remove the two remaining screws securing the PCB (Figure 6-29).
9. Install the two screws heat sink side first, then the two remaining screws securing the cover.
10. Reassemble in reverse order of the above steps.
6. Install new 12 volt power supply in reverse order of the above steps.
6. Install new 24 volt power supply in reverse order of the above steps.
3. Remove the screw securing the photo sensor and remove (Figure 6-35).
4. Remove two screws securing the light balancing filter (Figure 6-38).
5. Install new light balancing filter in reverse order of the above steps.
3. Remove two recessed screws securing the light balancing filter (Figure 6-40).
4. Install new light balancing filter in reverse order of the above steps.
3. Remove the two screws from the rail cover and remove cover (Figure 6-42).
4. Loosen the screw to the belt and remove belt Figure 6-43).
5. Remove two screws securing motor (Figure 6-44). Remove and unplug motor.
4. Remove two screws securing the cover over the analog board
5. Carefully remove fiber from mounting clips (Figure 6-46).
6. Install new optical fiber assembly in reverse order of the above steps.
7. When re-installing fiber into filter wheel housing, ensure that end is protruding 2 mm from plate (Fig-
ure 6-47).
Section 7
Adjustments, Calibration and Checks
7.1 Introduction
This section provides the adjustments, calibration and checks applicable to the Model 680 and Model 680 XR
Microplate Readers.
3. Set the jumper on the CPU Board (#281) per the following table:
Instrument
Jumper Jumper Placement
Configuration
6. Adjust trim pot VR1 until the voltage measures 0.000V +/- 0.001V.
7. Connect cable into Incubation Adjustment Tool, 116.47 ohm connector on tool.
8. Measure voltage between TP5 and TP0.
9. Adjust trim pot VR1 until the voltage measures 5.528V +/- 0.006V.
10. If voltage is out of range, repeat steps 3 through 9.
4. Remove two screws securing the belt cover as shown in Figure 7-6.
5. Loosen the screw and adjust the belt tension. Tension should be firm but not overly tight.
5. Use QC software to move plate beneath optics path per Section 7.7.3.
6. Check that the light is centered about the holes in the Checkmark Plate in columns 1 and 12 (Figure
7-9).
7.6 Firmware
7.6.1 General
1. Downloading firmware is achieved through a PC (Windows 95, NT, 2000, XP) and an RS-232 cable.
2. The communication requirements are as follows:
RS232C
Port: COM1
Baud Rate: 9600, 57600
Data: 8 bit 1 stop
3. The downloading program and additional files are required for downloading:
m680_up.exe mscomctl.ocx
comdlg32.ocx msvbvm60.dll
mscomm32.ocx asycfilt.dll
comcat.dll mci32.ocx
oleaut32.dll olepro32.dll
vb6stkit.dll
4. The current firmware files are:
Compatibility
Instrument Firmware
History Original New Backlit
Style Version
LCD LCD LCD
Original LCD 1.59 Initial release (compatible with serial num- Yes No No
Serial Numbers bers 10001 through 10735)
10001 - 10735 1.60 Minor corrections (compatible with serial Yes No No
numbers 10001 through 10735)
1.62 Added TSE protocols (compatible with serial Yes No No
numbers 10001 through 10735)
1.63 Changed TSE validation criteria (compatible Yes No No
with serial numbers 10001 through 10735)
1.71OL Added functionality for ESC/P printer. Yes No No
Added protocols for TSE Sheep/Goat and
Rabies (compatible with serial numbers
10001 through 10735)
New LCD 1.60NL Released for new LCD display (SN 10736 No Yes No
Serial Numbers through 13850). Minor corrections.
10736 - 13850 1.60cNL Released for Chinese version readers (SN No Yes No
11201 through 13850).
1.62NL Added TSE protocols (compatible with serial No Yes No
numbers 10736 through 13850)
1.63NL Changed TSE validation criteria (compatible No Yes No
with serial numbers 10736 through 13850)
1.71NL Added functionality for ESC/P printer. No Yes No
Added protocols for TSE Sheep/Goat and
Rabies (compatible with serial numbers
10736 through 13850)
Model 680XR 1.63XR For Model XR functionality only No Yes No
Backlit LCD 1.70 Released for backlit LCD and integration of No No Yes
Serial Numbers English and Chinese version firmware.
13851 and higher Added functionality for ESC/P printer (effec-
tivity serial number 13851 and higher).
Note: With firmware version 1.70, the English and Chinese versions have been integrated. A language choice
has been added with this version.
1. To enter the “Select Language” screen, press the “0” key and “4” key and turn ON instrument power.
Hold for approximately 5 seconds and the “Select Language” screen will appear.
2. Use Up and Down arrow keys to select language choice. Press Enter.
3. Reader resets automatically and restarts with the selected language.
Note: If the language selected is different, the reader will clear all memory and restore default values.
2. Click on “Model 680 Update” and the following screen will appear:
7. Press Yes to update to new version. If the instrument is at the latest version, the screen will indicate
no update is required.
8. The screen shown in Figure 7-15 will indicate the status as existing data and settings are retrieved
from the Model 680 reader.
10. The following steps are also indicated on the screen shown in Figure 7-16
11. Turn OFF the Model 680 Reader.
12. While pushing both “Main” and “0” key on the keypad, turn on reader power switch. After about 10
seconds, the following screen on LCD appears:
Firmware update
Are you sure?
Yes: “Start” Key
No: “7” Key
13. Push “Start/Stop” key switch on the Model 680 reader to begin reader firmware erasing.
14. After the erasing finishes, the LCD screen “RS Receive” will appear.
15. Click the “Start” button on PC screen. The firmware will begin uploading and bar graph at the bottom
of the screen will display status. This will take approximately 7 minutes.
16. When the firmware update operation is complete, the LCD on the reader will displayed “Finished”
and the restore previous data screen will appear (Figure 7-17).
17. Turn OFF reader power switch then turn ON. A “Battery Low” error message will occur. This is nor-
mal; press Enter.
18. At the Calendar settings screen, enter Date and Time and press Enter.
19. Enter password and press Enter.
20. After completion of log-in press the Yes button on the PC screen.
21. The restoring data screen will appear (Figure 7-18).
22. When the update is complete, the firmware complete message will appear (Figure 7-19).
7.7 QC Software
Note: There are 4 modes in this program; Measure Mode, Monitor Mode, Extension Mode and Incubator. The
instructions in this section apply to the Monitor Mode which allows step-by-step positioning of the plate carrier.
QC 1: Measure Mode
2: Monitor Mode
3: Extension Mode
4: Incubator
Figure 7-21: QC Mode
MON: V
DEC:
CH: A PLT: 12
FL: 1 Gain=1.1.1.0
Figure 7-22: Monitor Mode
Definitions:
MON = AD Value (voltage conversion)
DEC = AD Value (decimal)
CH = Row Position
PLT = Plate Column Position
FL = Filter Number
Gain = Gain A, B
Commands:
Main = Back to QC Mode Screen
Change of Gain A
Change of Gain B
Change of Plate Position
Change of Filter Position
“.” (Dot) = Change of Channel
Note: The Model 680 Reader with firmware version 1.70 added support for ESC/P external printer mode.
Previous versions of firmware support only ASCII print mode.
1. To change the external printer print mode, perform the following steps:
Section 8
Troubleshooting
8.2 Troubleshooting
The following troubleshooting tables are intended as a guide to assist in diagnosing some common problems.
Will not communicate with com- Improper COM Port selected Check that COM1 port is used.
puter
Improper serial cable Check for proper serial cable .
Blank LCD upon Power Up Fuse Blown Check Fuse. If bad, replace
Will not print to external printer Check printer setup Section 7.8
(ESC/P requires firmware ver-
sion 1.70 or later)
A/D Conversion Error Loose cable connection Check cables to CPU board and
Analog Board
Bad A/D convertor Replace Analog Board.
Grinding Sound or Plate Error Carrier binding Check for source of binding.
Clean and lubricate plate carrier
rods as necessary.
Code 8072 - Parameter Out of Received command parameter is Resend command and parame-
range out of range ter
Code 8073 - Not in Remote Instrument is not in Remote Send the “AQ” command to
Mode Mode acquire Remote Control Mode
Code 8075 - Filter Disk Jammed CPU board bad Replace CPU board
Code 8076 - Carrier Jammed Plate is not properly seated Reseat plate
After Reading
Interference or blockage Check for Interference or block-
age
Code 8082 - Filter Wheel Filter wheel jammed Check filter wheel
Code 8083 - Incubator Error Thermo sensor damaged Replace plate carrier
Code 8084 - Carrier Home Sen- Photo interrupter for plate carrier Replace photo interrupter
sor Closed Status Error damaged
CPU board bad Replace CPU board
Code 8085 - Carrier Home Sen- Plate is not properly seated Reseat plate
sor Open Status Error
Interference or blockage Check for Interference or block-
age
Code 8086 - Carrier Jammed Plate is not properly seated Reseat plate
Before Reading
CPU board bad Replace CPU board
Code 8087 - Plate Carrier Posi- Plate carrier jammed after read- Check plate carrier for obstruc-
tion ing tion.
Code 8089 - Filter Missing Filter missing from filter wheel Check filter wheel and install filter
allowing light to exceed accept-
able range
Code 8090 - Security Code in Security code did not match Remove security lock
Error
Code 8091 - RS-232C Error Data communication problem Check RS-232 cable
Code 8092 - A/D Conversion Bad A/D convertor Replace Analog Board
Error
Code 8093 - Stop Key Pressed Stop key pressed during mea- User error. Reset command
surement
RS-232C Error Parity, Overrun or Framing errors Ensure RS-232 is properly con-
occurred nected. Repeat download proce-
dure
Verify Error Communicated data and written Repeat download procedure
data is different (make sure instrument power is
cycled)
Flash Write Error Flash memory could not be writ- Repeat download procedure
ten (make sure instrument power is
cycled)
Flash Erase Error Flash memory could not be Repeat download procedure
erased (cycle power; first screen to
appear is downloading screen)
Battery Error Occurs after firmware download Normal. Press Enter. Reset date,
time and filter data.
Fails Accuracy, Linearity or Erratic or non-uniform well-to-well Check for cleanliness of optics
Precision OD values and lenses. Clean as required.
Check lamp. Reseat or replace
as necessary.
Belt tension too tight or too loose Check belt tension and tighten/
loosen as necessary per Section
7.4
Failed Cross Talk Wells A2, C2, E2, G2, B3, D3, F3, Check for cleanliness of optics
and H3 are exceeding 0.003 OD and lenses. Clean as required.
due to some light path blockage.
Check lamp. Reseat or replace
as necessary.
Fails IR Blocking Detected IR light passing through Check filter for damage or deteri-
plate; not blocked by filters ation. Replace as necessary.
Section 9
Parts Information
You are an integral part of our inventory process. When you return parts or assemblies to Bio-Rad to be eval-
uated and repaired, please attach a tag to each assembly. The tag should contain the following information:
• Model and serial number of the unit which the part was removed
• The part number or catalog number of the part being returned
• The date of removal
• A description of the problem or symptoms exhibited
• Your name and other identification information
This information will help our service centers to make repairs efficiently. When you receive replacement parts,
save the packaging material for returning the defective parts for repair.
Table 9-1: Replaceable Parts for the Model 680 and 680 XR Microplate Reader
Table 9-1: Replaceable Parts for the Model 680 and 680 XR Microplate Reader
Door 9310164
Fan 9310168
Table 9-1: Replaceable Parts for the Model 680 and 680 XR Microplate Reader
Table 9-1: Replaceable Parts for the Model 680 and 680 XR Microplate Reader
Table 9-1: Replaceable Parts for the Model 680 and 680 XR Microplate Reader
Table 9-1: Replaceable Parts for the Model 680 and 680 XR Microplate Reader
Table 9-1: Replaceable Parts for the Model 680 and 680 XR Microplate Reader
Table 9-1: Replaceable Parts for the Model 680 and 680 XR Microplate Reader
Table 9-1: Replaceable Parts for the Model 680 and 680 XR Microplate Reader
9.5 Filters
The following is a list of custom filters available:
9.6 Tools
The following is a list of special tools and software required for servicing the Model 680 Microplate Reader.
Firmware 1002446
9.7 Manuals
The following is a list of reference manuals.
1709523
(USB Hasp)
Section 10
Drawings
10.2 Drawings
• Block Diagram
• Wiring Diagram
AC Input Microplate
Photo Diodes
Analog Board
Power Supply ( Amplifier )
( A/D converter )
Power Supply
(Option)
Internal Incubation
Printer
(Option)
(Option) LCD & Key Switch
1 2 3 4 5
1 1
Inlet
Power SW Fuse
Power Supply
4p
5p
L N
12V
CN2
CN1
E
No.283-2
Option
Lamp Membrane SW
Printer Dsub 25p Dsub 9p
Control LCD
CN43 CN22
Board
2 2
CN45
CN44
Internal
30p
25p
Printer
12p 20p
CN62 CN63
No.284 No.283-1
CN61
Fan 34p
Option
PT Sensor
30p 26p 9p 3p 2p 34p
Incubation CN42 CN04 CN02 CN01 CN33 CN06 M1
CN10
CN51
10p
6p
CN05
Control
No.286-2
3 3
Board (Plate Carrier)
CN52
CN54
5p
5p
M2
CN11
No.286 Shield Case
7p
Heater
(Filter)
CN53
2p No.281 PI1
3p
CN07
4p CPU Board (Plate Carrier)
CN2a
CN1a
5p
4 Power Supply 4
CN09
PI2
4p
24V CN08
34p
(Filter)
34p
A
CN81 Base Plate
B
C
No.282 Measurement
D
5 E 5
Photo Title
Analog Board Diodes F
/QFGN/KETQRNCVG4GCFGT
9KTKPI&KCITCO
G Document Number Rev
Reference H
$4' ;CUWUJK-QEJK[COC
Date: June 4, 2001 Sheet 1 of 1
1 2 3 4 5
Appendix
Appendix
100-2414C 03/04
Appendix A
System Checkout Air Read (all wells < 0.005) Pass Fail
The above referenced instrument has been tested and meets all performance specifications.
______________________________ _____________
Bio-Rad Date
Appendix B
System Checkout Air Read (all wells < 0.005) Pass Fail
The above referenced instrument has been tested and meets all performance specifications.
______________________________ _____________
Bio-Rad Date
Appendix C
Appendix C
The Checkmark program is used with the Checkmark Reader Performance Check Plate for validating the
performance of the Model 680 Microplate Reader. It will test reader accuracy, precision and linearity. In
addition it will provide information on plate positioning, cross-talk, filter wavelength, overflow and 0 OD, and
infrared (IR) blocking for diagnostic purposes.
The Bio-Rad Catalog number is 170-6940. The Checkmark program and Check Plate is manufactured by:
Memory error
Calendar error
Printer error
Battery error
Printer error
Filter missing
Error code : 8089
Appendix D – Error Flow Diagrams