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INTRODUCTION

In Guimaras, problems occur when subterranean termites attack the wooden elements of

human-made structures, homes, business and warehouses of fruits like mangoes. There has been

an increasing interest for the development of environment- friendly, botanical pesticides, microbial

sprays, and insect growth regulators. Subterranean colonies were in the ground beneath or near

the school, before it was built. The termites are coming into the school buildings through the

utilities holes another cracks and gaps in the concrete slab and foundations. These pests caused

destruction of school textbooks and other learning references which affects the teaching and

learning process in school. Many plant extracts and essential oils are alternative sources of termite

control agents because they constitute a rich source of bioactive chemicals (Cheng et al., 2007).

Milletia piscatoria locally known in Guimaras as “tubli” is a vine plant having roots that

possess insecticidal properties because of its rotenone content. Rotenone from roots is a raw

material for insecticides against plant pests. Since early times, the root has been used as fish poison

in the Philippines, the Dutch East Indies, and the Malay Peninsula. The Chinese and Malay

gardeners have used the root decoction as an insecticide. Rotenone has also been found effective

against plant lice, leaf beetles, aphids, flies, caterpillars, ticks, chicken lice, red spiders, and other

insects (Quigley, 2009).

In this study, the bio-termiticidal activity of Milletia piscatoria leaf extract on the mortality

of subterranean termites was evaluated. The significance of the potential bio- termiticide is in many

aspects - cost, renewability, and environment friendly.

MATERIALS AND METHODS

Collection of Leaves

Young leaves of Milletia piscatoria were gathered at Tastasan, Buenavista, Guimaras.

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Preparation of Leaf Samples

250 grams Milletia piscatoria leaves were cut into smaller sizes and were pounded using

mortar and pestle.

Figure 1. Pounding of Milletia piscatoria Leaf sample

Photo taken by: Lurela T. Ferasol

Leaf Extraction

The cut leaves of Milletia piscatoria were placed in a clean cloth and were squeezed to

obtain the extract.

Figure 2. Extraction of Milletia Piscatoria leaf extract

Photo taken by: Myra P. Gaitano

Collection of Subterranean Termites

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Subterranean Termites from the same mound were gathered from Tastasan, Buenavista,

Guimaras.

Figure 3. Collected Subterranean Termites

Photo taken by: Myra P. Gaitano

Adaptation of Subterranean Termites in artificial environment and Preparation of Set- up

Eighteen (18) plastic cups were prepared. A filter paper was cut at a size just enough to

exactly fit at the bottom part of the specimen cup. Ten (10) subterranean termites were carefully

transferred to the specimen cup with a filter paper. Subterranean Termites were allowed to adapt

to the new environment (inside the cup) for 24 hours.

Figure 4. Transferring of subterranean termites to the

plastic cups with filter paper
Treatments Photo taken by: Olga Mare S. Barredo

 Treatment 1 (25%) - 10 termites, 2.5 mL Leaf Extract, 7.5 mL Water

 Treatment 2 (50%) – 10 termites, 5 mL Leaf Extract, 5 mL Water

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 Treatment 3 (75%) – 10 termites, 7.5 mL Leaf Extract, 2.5 mL Water

 Treatment 4 (100%) – 10 termites, 10 mL Leaf Extract

 Treatment 5 (negative (-) control) – 10 termites, 10 mL Distilled Water

 Treatment 6 (positive (+) control) – 10 termites, 10 mL Kerosene

Figure 2. Extraction of Milletia Piscatoria leaf extract

Photo taken by: Myra P. Gaitano

Figure 5: Experimental Set-up

T1- R3 T4- R1 T4- R3 T2- R3 T1- R1 T3- R2

T5- R1 T6- R1 T5- R2 T6- R2 T2- R1 T5- R3

T3- R3 T6- R3 T3- R1 T2- R2 T1- R2 T4- R1

Application of Treatments

Each treatment of the three (3) replicates was gradually dropped to each specimen cup

(without hitting the termites) with subterranean termites until the filter paper was completely wet.
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A dropper was used to drop 1.5 mL of every treatment to completely wet the filter paper. It was

allowed to expend for 24 hours.

Figure 6. Extraction of Milletia Piscatoria leaf extract

Photo taken by: Myra P. Gaitano

Data and Data Gathering Procedure

The response and mortalities of the subterranean termites were observed for 24 hours as

they moved freely in the filter paper (wet with the Milletia piscatoria extract). A magnifying glass

was used to closely observe the subterranean termites.

Mortality was counted to determine the response of subterranean termites to Milletia

piscatoria leaf extract.

Cleaning and Disposal

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After the experiment, the used apparatus were washed, sterilized and returned to the cabinet

for safe keeping.

Used samples and other non-toxic waste materials were disposed at Buenavista National

High School disposal area. Toxic materials were disposed at Buenavista National HighSchool

Science Laboratory following the proper disposal procedures.

Statistical Analysis Procedure

The data were interpreted and analyzed using descriptive statistics. Mean was used to

describe the mortality rate of the subterranean termites as response to Milletia piscatoria leaf

extract.

RESULTS

The way subterranean termites responded to the Milletia piscatoria leaf extract which was

present in the filter paper where they crawled indicated that Milletia piscatoria leaf extract was a

potential termiticide. Counting of mortality was based on immobility and the unresponsiveness of

the subterranean termites to stimulus.

The varying concentrations of Milletia piscatoria leaf extract caused the mortalities of

subterranean termites after 24 hours of exposure. These results were compared to the mortalities

of subterranean termites caused the positive control (Kerosene) and the negative control (distilled

water).

Table 1 shows that 25%, 50%, 75% and 100% Milletia piscatoria leaf extract totally

eliminated the subterranean termites after 24 hours. Kerosene, which was used as positive control
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caused the mortalities of 10 subterranean termites after 24 hours while 10 subterranean termites

which were treated with distilled water (negative control) survived after 24 hours.

Table 1.Mortality of Subterranean Termites after 24 hours

Mortality
Treatments Mean
R1 R2 R3

25 % Milletia piscatoria Leaf Extract

10 10 10 10
50 % Milletia piscatoria Leaf Extract
10 10 10 10
75 % Milletia piscatoria Leaf Extract
10 10 10 10
100 % Milletia piscatoria Leaf Extract
10 10 10 10
100 % Distilled water (negative control)
0 0 0 0
100 % Kerosene (positive control) 10 10 10 10

DISCUSSION

Results of the experiment showed that Milletia piscatoria leaf extract had

components which caused the mortality of subterranean termites. The four (4) different

concentrations exhibited a promising termiticidal effect. All treatments (25%, 50%, 75% and 100%

Milletia piscatoria leaf extract) exhibited a promising performance as bio-termiticide. This was

strongly supported by the equal mean mortality caused by the treatments as compared to the

mortalities caused by kerosene, the positive control.

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There was no recorded mortality in the 100 % distilled water, negative control in the study.

This simply proves that the mortalities of subterranean termites in 25%, 50%, 75% and 100%

Milletia piscatoria leaf extract really cause by the component(s) found in the extract.

The results provided a strong indication that Milletia piscatoria leaf extracts could be

used as bio-termiticide. These also suggest that the use of commercial termicides could be

substituted by the leaf extract of this plant.

CONCLUSION

Milletia piscatoria leaf extract with 25%, 50%, 75% and 100% concentrations exhibited

effective bio-termicitidal activity against subterranean termites. Results led to formulation of

termite control solution, which used the studied leaf extracts as a way of caring the environment.
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RECOMMENDATIONS

The researcher strongly recommends:

1. the use of bio-termiticidal leaf extracts from Milletia piscatoria;

2. the leaf extract to undergo phytochemical testing;

3. the screening of bio- termiticidal potency of the plant extract in different methods; and

4. the testing of plant extracts to other insects and pests.

REFERENCES
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Baatuuwie , B and Aalangdong, O (2012), Indigenous knowledge of termite control: A case

study of five farming communities in Gushegu District of Northern Ghana. Retrieved

October 14, 2016 from https://pubs.ext.vt.edu/444/444-500/444-500_pdf.pdf

Cheng SS, Chang HT, Wu CL, Chang ST (2007). Antitermitic activities ofessential oils from

coniferoustrees against Coptotermes formosanus. Biores. Retrieved October 12,2016 from

https://www.researchgate.net/publication

United Nations Environment Programme(2000). Chemicals finding alternatives to persistent

organic pollutants (pops) for termite management. Retrieved October 14, 2016 from

http://www.unep.org/chemicalsandwaste/Portals/9/Pesticides/Alternatives-termite-

fulldocument.pdf.

Upadhay, R. et.al (2010). Anti-termite control of tropical and dessert plants.Retrieved October

14, 2016 from http://www.biotechphils.com/blog/termite-control-philippines-blog-

termites and termite control.

Quigley, C. (2009). Aboriginal Fish Poisons. American Anthropologist, Volume 58, Issue 3.

Retrieved October 14, 2016 from

http://onlinelibrary.wiley.com/doi/10.1525/aa.1956.58.3.02a00090/pdf.
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ACKNOWLEDGEMENT

Utmost gratitude is extended to the following persons:

Mr.Vizur-TY C.Gaitano and Mrs. Ma. Myra P. Gaitano, parents of the researcher;

Mrs. Love Grace H. Velasco, School Principal II, Buenavista Central School;

Miss Jocelyn V. Villa, School Principal II, Buenavista National High School;

Mrs. Lurela T. Ferasol, researcher’s adviser;

Mr. RoeyFerasol, researcher’s grammarian;

Miss Olga Mare S. Barredo;

All teachers of Buenavista Central School; and

Above all, to the Almighty God, for the strength and wisdom provided to the researcher.