Saracho, Ezra Joseph G.

Domingo, Alvin

Caranza, Jan Jair

ABT 106 – B2L

Exercise 9
Table 1 Summary of Disease incidence if Backcross Population and Parentals

PERCENT Transf Transf Transf Transf Transf Transf

BC1
DISEASE BC1 BC1 orme orme orme orme orme orme
BC1F1 INCIDENCE (%)
F3:
F3:1 F3:1 d d d d d d
Family 21 DAE 1
ID No. BC1F3 BC1F3 BC1F3 BC1F3 BC1F3 BC1F3
:1 :2 :3 :4 :5 :6
Rep Rep Rep Tot Mea Vari Varian
Rep 1 Rep 2 Rep 3 Total Mean
1 2 3 al n ance ce
62.3 207 27.0
1 75
8
70.2 69.2 1.88 1.80 1.85 5.52 1.84 0.00
.59 5
97.9 45.5 188 62.9 612.
2
2
45.3
3
1.99 1.66 1.66 5.31 1.77 0.02
.75 2 56
81.7 33.7 159 53.2 425.
3
9 7
44.2 1.91 1.53 1.65 5.09 1.70 0.03
.75 5 29
68.2 60.4 94.4 223 74.3 211.
4
1 8 4
1.83 1.78 1.98 5.59 1.86 0.01
.13 8 3
88.6 37.6 23.8 150 50.0 777.
5
4 2 1
1.95 1.58 1.38 4.90 1.63 0.06
.07 2 3
78.9 53.0 181 60.6 168.
10
5 1
50 1.90 1.72 1.70 5.32 1.77 0.01
.95 5 87
58.0 47.3 58.8 164 54.7 27.3
11
4 6 4
1.76 1.68 1.77 5.21 1.74 0.00
.25 5 9
25.8 17.7 13.8 57. 19.1 24.9
16
8 8 9
1.41 1.25 1.14 3.81 1.27 0.01
54 8 4
29.5 96. 32.2 42.7
17
5
26 41.3 1.47 1.41 1.62 4.50 1.50 0.01
85 8 8
55.9 66.9 185 61.7 20.3
18 62.5
5 2
1.80 1.75 1.83 5.37 1.79 0.00
.38 9 1
53.2 64.1 139 46.6 311.
20
3
22.5
7
1.73 1.35 1.81 4.89 1.63 0.04
.9 3 12
57.3 45.5 46.6 149 49.8 28.3
23
5 5 7
1.76 1.66 1.67 5.09 1.70 0.00
.57 6 2
14.7 16.6 68. 22.9 106.
26 37.5
1 7
1.57 1.17 1.22 3.96 1.32 0.03
87 6 38
53.9 68.1 79.6 201 67.2 109.
27
8 8 2
1.73 1.83 1.90 5.47 1.82 0.00
.77 6 98
 38.8 63.8 19.3 122 40.7 332.
30
9 9 5
1.59 1.81 1.29 4.68 1.56 0.05
.12 1 34
82.9 62.2 79.1 224 74.7 80.6
31
1 5 5
1.92 1.79 1.90 5.61 1.87 0.00
.31 7 7
68.5 47.4 33.8 149 49.9 202.
33
1 9 9
1.84 1.68 1.53 5.04 1.68 0.02
.88 6 84
39.8 11.1 20.9 71. 23.9 142.
34
6 1 2
1.60 1.05 1.32 3.97 1.32 0.05
89 6 32
51.1 44.5 37.9 133 44.5 29.0
35
7 9 7
1.71 1.65 1.58 4.94 1.65 0.00
.73 8 4
60.8 54.5 49.0 164 54.8 23.3
36
7 7 5
1.78 1.74 1.69 5.21 1.74 0.00
.49 3 3
24.3 115 38.5
40 40.1
8
51.3 122 1.60 1.39 1.71 4.70 1.57 0.02
.78 9
68.2 31.7 61.8 161 53.9 253.
41
1 5 7
1.83 1.50 1.79 5.13 1.71 0.02
.83 4 06
50.5 58.6 58.3 167 55.8 14.2
43
1 6 3
1.70 1.77 1.77 5.24 1.75 0.00
.5 3 1
76.7 67.6 52.8 197 65.7 96.6
44
4 9 9
1.89 1.83 1.72 5.44 1.81 0.00
.31 7 9
72. 59. 43. 175 58.3 143.
45 1.86 1.78 1.63 5.27 1.76 0.01
35 7 1 .16 9 48
73.0 50.0 43.6 166 55.5 159.
48
2 6 2
1.86 1.70 1.64 5.20 1.73 0.01
.7 7 26
41.1 14.2 125 41.8 517.
52 70
8 9
1.85 1.61 1.16 4.61 1.54 0.08
.46 2 55
41.9 42.4 77.0 161 53.8 270.
55
7 5 7
1.62 1.63 1.89 5.14 1.71 0.02
.5 3 14
44.6 63.6 46.3 154 51.5
57
4 2 5
73.5 1.65 1.80 1.67 5.12 1.71 0.00
.61 4
65.3 48.1 147 49.1 164.
64 34
7 7
1.53 1.82 1.68 5.03 1.68 0.01
.54 8 53
12.1 49.8 51.3 113 37.7 328.
67
2 1 2
1.08 1.70 1.71 4.49 1.50 0.09
.25 5 77
51.5 42.0 168 192.
72
9 1
75 56.2 1.71 1.62 1.88 5.21 1.74 0.01
.6 05
46.0 29.2 30.2 105 35.1 59.2
76
6 8 1
1.66 1.47 1.48 4.61 1.54 0.01
.55 8 8
73.3 39.4 176 58.8 203.
81
3
63.7
2
1.87 1.80 1.60 5.27 1.76 0.01
.46 2 57
36.7 80.3 193 64.4 386.
82 76.3
6 6
1.88 1.57 1.91 5.35 1.78 0.02
.42 7 63
54.7 40.9 125 41.9 102.
83
9 5
30 1.74 1.61 1.48 4.83 1.61 0.01
.74 1 9
55.8 53.0 45.6 154 18.4
84
2 4 4
51.5 1.75 1.72 1.66 5.13 1.71 0.00
.51 6
 54.7 44.1 20.2 119 39.7 208.
87
6 6 5
1.74 1.65 1.31 4.69 1.56 0.03
.17 2 34
75.6 48.1 30.2 154 51.3 347.
88
1 5 9
1.88 1.68 1.48 5.04 1.68 0.03
.04 5 47
65.5 46.1 28.5 140 46.7 228.
92
8 8 7
1.82 1.66 1.46 4.94 1.65 0.02
.32 7 41
31.5 38.6 95. 31.7
94
8 4
25 31 1.50 1.59 1.40 4.48 1.49 0.01
22 4
237 187 190
Rep
9.8 2.0 9.6
Total®
3 3 4
Grand 616
Total 1.4
(G) 6
50.0
Grand
929
Mean
3
89.5
20.8 19.2 40. 13.3
P2 3 3
0.00 990
06 5
(Res) 4
31.4
86.6 82.6 96.0 265 88.4
P1 7 1 0
288
.28 3
(Susc) 6

Figure 1 Scatterplot of Backcross Population

Figure 2 Histogram Chart of Transformed Backcross Population

Table 2 ANOVA of Disease Incidence of Backcross Population (0.05%)

Source of
Variation SS df MS F P-value F crit
Replication 20932.91 40 523.3228 2.206875 0.00134 1.544887
Genotype 3905.317 2 1952.658 8.234446 0.00056 3.110766
Error 18970.64 80 237.133

Total 43808.87 122

30.74 8.91
CV R2

Table 3 ANOVA of Disease Incidence of Backcross Population (0.01%)

Source of
Variation SS df MS F P-value F crit
Replication 14989.07 40 374.7268 1.84453 0.028109 2.114232
Genotype 3144.644 1 3144.644 15.47899 0.000324 7.3141
Error 8126.227 40 203.1557

Total 26259.94 81
28.45 11.97
CV R2
Table 4 Histogram Summary of Disease Incidence of Backcross Population and Parentals

bin Frequency P1 P2
10 0
20 1 1
30 2
40 6
50 9
60 14
70 6
80 2
90 0 1
100 0
More 0

HISTOGRAM
Frequency P1 P2
14
FREQUENCY

9
6

6
2

2
1
1

1
0

10 20 30 40 50 60 70 80 90 100
BIN

Figure 3 Histogram Chart of Backcross Population and Parentals

Answer to questions

3.a. The frequency distribution somewhat follows a normal bell curve. Highest population in a range
is in between 50 to 60 with a population of 14. The susceptible parental (P1) is in between range 10 to
20 and the resistant parental (P2) is in between range of 80 to 90. The frequency distribution is not
symmetrical favoring the lower mean.
3.b. The variances are heterogenous and does not follow a linear pattern. The variance and mean
are not related. There is too much deviation between the expected pattern between the variance and
mean.

3.c. Transforming the variance by logarithmic transformation. The data is still somewhat
heterogenous but the trend is now clearly visible. The pattern follows a linear horizontal line.

4.a There is significant level of variation in all data except for the replication data for 1%
significance. It has a p value of 0.02

4.b. Yes, it is important. Only the 1% significance replication shows that it is not significant. This
signifies that the data for replication has significant value.

4.c. Coefficient of variation is a measure of distance between data points. It can describe how far
each data from each other.

4.d. R2 is a measure on how close the data points are to the regression line. The regression line is a
line which best describes the trend of the data points. This can help describe if the effects of the gene to
the phenotype follows a trend.

4.e. The higher the R2 value, the more predictable the effect of the QTL to the trait of an organism. A
usable trend can be used. The higher the R2, the more a trend can be formed. The QTL can more
accurately be used determined.