Results and Discussion Dan Banh

Data Table

Tube Protein Concentration Absorbance Average of Abs Net Absorbance

1 0 0.016
2 0 0.017 0.0165 0
3 2000 0.539
4 2000 0.548 0.543 0.526
5 1600 0.548
6 1600 0.535 0.541 0.524
7 800 0.283
8 800 0.292 0.287 0.27
9 400 0.172
10 400 0.168 0.17 0.153
11 200 0.101
12 200 0.104 0.102 0.085
13 397 0.152
14 397 0.162 0.157 0.14
15 291 0.126
16 291 0.13 0.128 0.111
17 69 0.033
18 69 0.024 0.0285 0.012

Graph

BCA Assay Standard Curve

0.6
f(x) = 0x + 0.03

0.5

0.4
Net Absorbance

0.3

0.2

0.1

0
0 500 1000 1500 2000 2500
Protein Concentration (ug/ml)
Post Lab Questions
1. What is the protein concentration of your sample in the assay?
• Base on the trend line equation, three unknown samples of the diluted protein concentration are
of the following: 397ug/ml, 291 ug/ml, and 69ug/ml.

2. What is the protein concentration of your unknown tube sample?

• 397ug/ml * 5 = 1985 ug
• 291 ug/ml *10 = 2910 ug
• 69 ug/ml * 20 = 1380 ug
3. Discuss why you diluted your unknown sample, whether or not this was necessary.