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Materials:
Loading buffer, 5X
Parafilm
Ethidium Bromide (EtBr) solution (10mgm1), Scoop, Stained and Destained tray
Microcentrifuge racks
Gloves
Equipment:
Micropipette set
Freezer
Vortex
Imager system
We centrifuged at 12 000-15 000 rpm for 10 min at room temperature. We carefully removed
the liquid from the tube and add 1m1 of 70, ethanol (- 20°C) followed by vortex. Then, we
centrifuged again for 2 min and aspirated the supernatant. The supernatant was removed as
much as we can and followed by drying in a vacuum for 5-10 min. We dissolved the precipitate
in TE buffer with the suitable volume.