Republic of the Philippines

National Capital Region

Division of City Schools
Batasan Hills National High School
IBP Road, Batasan Hills, Quezon City
SY 2018-2019

An individual science investigatory project entitled:

Experimenting the Bactericidal Capability of Coleus blumei (Mayana) Leaves against S.

aureus, K. pneumoniae and P. aeruginosa

Carlo F. Agas

Researcher

Ms. Cristine Sañada

Research Adviser

Ferbruary 2019

Research Expo 2019 Theme: “Diffusing Cognition, Yielding Reactions.”

 ACKNOWLEDGEMENT

This scientific investigatory project wouldn’t be possible without the support and help of

those who took time just to help, give ideas and motivation. First of all, The researcher would like

to give tanks to our Almighty God Jehovah for giving us such wisdom and guidance

unconditionally. The researcher would also like to thank his parents and his family who supported

me on doing this SIP financially and morally. The researcher would also like to express his

gratitude towards his research adviser, Ms. Cristine S. Sañada, for guidance and assistance

throughout the whole time. Thanks to Mrs. Mary Grace Lumame and Ms Michele Hababag,

the laboratory custodians of the chemistry and physics laboratory for allowing the researcher to

use such facilities for the sake of the experimentation of the study. Thanks also to Nicole Anne

Alayon for letting the researcher borrow equipment for experimentation such as blender. The

researcher would also like to show recognition to the efforts of their class adviser, Mrs. Rubilyn

Diane Umali for all the motivation and support she gave us. And lastly to my friends, Zaki Adrian

T. Pinili, Ivy Katrina K. Malañgen, Franxine Thea T. Camacho, and Shaira Balanza, for

supporting and helping do this SIP by helping me with writing the paper and giving advices on

practical writing. Not just that but also helping me but also for giving me motivation and innovative

ideas that helped me throughout this endeavor.

C.F.A.

Research Expo 2019 Theme: “Diffusing Cognition, Yielding Reactions.”

Title: Experimenting the Bactericidal Capability of Coleus blumei (Mayana) Leaves Against S.
aureus, K. pneumoniae and P. aeruginosa
Name: Carlo F. Agas
Research Adviser: Ms. Cristine S. Sañada
Address: IBP Rd., Batasan Hills, Quezon City

ABSTRACT
A lot of people suffer from diseases such as strep throat, urinary tract infection,
tuberculosis, and skin diseases pneumonia, which are caused by various pathogenic microbes, one
of that being, bacteria. As these adversities occur and progress, it gave the researcher the resolution
to contribute in resolving the problem by conducting this significant research study. This project
aims to determine possible alternatives to commercial antibacterial substances without spending a
lot using crude leaf exract from Colues blumei. It was also conducted to test if the said substance
is effective against various bacteria. First, the researcher gathered leaves then brought some
samples to UP Diliman for Plant Identification to confirm the plant material. Then, the leaves were
extracted by simple extraction. After that, it was immediately brought to UP Diliman, Natural
Sciences Research Institute (NSRI)-Microbiological Department for an Antimicrobial Assay, a
test if the substance can show antimicrobial properties against other microbes. The microbes that
were used in the Assay were K. pneumoniae, P. aeruginosa, and S. aureus. The results showed
that the crude leaf extract wasn’t able to inhibit K. pneumoniae with an anti-microbial index of 0,
the same with P aeruginosa with an anti-microbial index of 0. On the other hand, it was able to
inhibit S, aureus with an anti-microbial index of 1.6. While the controlled sample which is the
Chloramphenicol disc had a clearing zone of 5.3, 1.5, and 4.5 to the said bacteria respectively. As
what has been showcased, it can be concluded that Coleus blumei crude leaf extract can’t inhibit
various bacteria. It can also be concluded that compared to the antimicrobial property shown by
Chloramphenicol, the crude leaf extract has a low antibacterial property. And another conclusion
is that, the crude leaf extract have a low to no effectivity to those bacteria that the crude leaf extract
failed to inhibit compared to S. aureus.

Keywords: anti-microbial index, S. aureus, K. pneumoniae, P. aeruginosa, Coleus blumei

Research Expo 2019 Theme: “Diffusing Cognition, Yielding Reactions.”