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Composition:
Milk is a mixture of lipids, carbohydrates, proteins and many
other organic compounds and inorganic salts dissolved or
dispersed in protein.
The most variable component of milk is fat followed by protein.
The composition of milk varies with the species, breed, diet,
lactational period and interval between milking along with
individual variation.
Fat:
The flavour of milk is due to milk fat.
Milk fat or butter fat is of great economical and nutritive value.
Buffalo’s milk contains 6.5% fat and cow’s milk contain 4.1%
fat.
Each fat globule is surrounded by a thin layer which is
composed of a lipid-protein complex and a small amount of
carbohydrate.
Milk fat contain about 64% fatty acids. Other lipid materials
present are phospholipids, sterols, free fatty acids, carotenoids
and fat-soluble vitamins.
Carotenes are responsible for the yellow colour of milk fat.
Geber test is used to know the percentage of gat present in
food.
Proteins:
Milk protein contains proteoses, peptones and milk enzymes.
1. Casein:
It constitutes 80% of the total nitrogen in milk
and contains 8.2% calcium and 5.7% carbs. It is
precipitated on the acidification of milk to pH
4.6 at 20°C.
It occurs as a colloidal protein calcium
phosphate complex.
It is also a glycoprotein. It is a good source of
essential amino acids.
It can be separated from milk by addition of
rennin.
White colour of milk is caused by reflection of
light by the colloidally dispersed casein,
calcium and phosphorous.
2. Whey proteins:
They are made of α- lactalbumin and β-
lactoglobulin, serum albumin,
immunoglobulins, enzymes and proteose-
peptones.
These are not precipitated but are coagulated
by hear.
Small amounts of lactoferrin and serum
transferrin are also present.
Whey protein concentrates which are
produced by ultrafiltration techniques are
given in lactose intolerance individuals.
Carbohydrates:
Milk contains 4-5% carbohydrate.
The chief carbohydrate is lactose which on
hydrolysis gives glucose and galactose.
Lactose has only one sixth sweetness of
sucrose.
When milk is heated, lactose reacts with
protein and develops a brown colour. (non
enzymatic browning- Maillard reaction)
Minerals:
Chlorides, phosphates, citrates, sulphates and
bicarbonates of Na, K , Ca and Mg are present
which influence the condition of stability of
proteins especially the casein fraction.
Cu and Fe are important in development of off
flavours in milk and milk products.
Trace elements like Zn, Mb and I are also
present.
Enzymes:
Enzymes found in milk originate from
mammary glands or may be released by
contaminating bacteria.
Alkaline phosphatase exists as lipoprotein and
is inactivated by normal pasteurisation
procedures. Its activity is tested to determine
the effectiveness of pasteurisation.
Milk lipase is responsible for the development
of rancid flavours in milk. Lipases may be
important in the development of desirable
flavours in some cheeses.
Xanthine oxidase is a conjugated protein
complexed with FAD, iron and Mb. The
enzyme degradation of FAD gives FMN and
riboflavin.