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6 Daily Operations

6 Daily Operations

6.1 Introduction
This chapter introduces the daily operations from the startup to the shutdown of the analyzer with a
focus on the detailed operation procedures for running samples in different working modes.
A flow chart indicating the common daily operation process is presented below.
Figure 6·1 Daily Operations Procedure

Preparation before
the operation

Startup of the
Startup instrument and the
terminal software

Daily Quality
Control

Sample Collection
and Handling

Sample Analysis

Report
Management

Shutdown of the
Shutdown terminal software and
the instrument

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6.2 Pre·operation Preparation

6.2.1 Equipment Inspection

All the samples, controls, calibrators, reagents, wastes and areas in contact with them are potentially
biohazardous. Wear proper personal protective equipment (e.g. gloves, lab uniforms, etc.) and follow
laboratory safety procedures when handling them and the relevant areas in the laboratory.

• Be sure to dispose reagents, waste, samples, consumables, etc. according to you local
legislations and regulations.
• The reagents are irritating to eyes, skin and mucosa. Wear proper personal protective
equipment (e.g. gloves, lab uniforms, etc.) and follow laboratory safety procedures when
handling them in the laboratory.
• If the reagents accidentally spill on the skin, wash them off with plenty of water and if necessary,
go see a doctor; if the reagents accidentally spill into the eyes, wash them off with plenty of water
and immediately go see a doctor.
• Keep clothing, hairs and hands away from the moving parts to avoid injury.
• The sample probe tip is sharp and may contain biohazardous materials. Exercise caution to
avoid contact with the probe when working around it.

• You should only use the BIOTA-specified reagents. Store and use the reagents as specified in
instructions for use of the reagents.
• Check if the reagents are connected correctly before using the analyzer.
• After long-distance transportation, the reagent should settle for more than one day before use.
• Be sure to use clean K2EDTA vacutainer blood collection tubes with anticoagulant, fused silica
glass/plastic test tubes, centrifugal tubes and borosilicate glass capillary tubes.
• Be sure to use the BIOTA-specified disposable products including vacutainer blood collection
tube, vacutainer blood collection tubes with anticoagulant and capillary tubes etc.

Perform the following checks before turning on the analyzer.


• Waste container
Check and make sure the waste container is empty.
• Fluidic tubing and power connections
Check and make sure the reagents and waste tubing are properly connected and not bent.
Check and make sure the power cord of the analyzer is properly plugged into the power outlet.
• Printer (optional)

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Check and make sure enough printer paper is installed, the power cord of the printer is properly
plugged into power outlet, and the printer is properly connected to the external computer.

• Keyboard, mouse and external computer


Check and make sure the network cable of the external computer is properly connected to the
analyzer.
Check and make sure the keyboard and the mouse are well connected to the external computer.

6.2.2 Tube and Barcode Preparation


Before running samples in AWB mode, you should prepare the BIOTA-specified tube racks and
tubes, and make sure the barcode labels meet the requirements.

Tube Specifications
The following types of vacutainer collection tubes should be used to collect whole blood samples.
• Ф13X75 (mm) (without the cap) vacutainer blood collection tube
• Ф12X75 (mm) (without the cap) vacutainer blood collection tube

• The height of the vacutainer blood collection tube with cap can not be higher than 83mm.
• The referred vacutainer collection tubes are not only available in AWB mode, but also available
in VWB and CWB mode.

Barcode Symbologies
The following barcode symbologies are intended to be used to make the tube barcode.
• CODE39
Code length: 1-20
• CODE93
Code length: 1-20
• CODE128
Code length: 1-20
• CODEBAR
Code length: 1-20
• CODE128
Code length: 1-20

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Barcode Specifications

The requirements for the barcode specification are as follows.


• Code height: d � 10mm
• Label width: a s 45mm
• Clear area: b � 5mm, C � 5mm
• Wide-to-narrow ratio: between 2.5:1 and 3.O:1
• Code quality: according to ANSI MH1O.8M standard, the code quality is greater or equal to C
level.

Grades of A, B, C, D, and F are assigned to evaluate the quality of barcode. The grade (G) of each
level is defined in specified range: A(3.5sGs4.0), B(2.5sG<3.5), C(1.5sG<2.5), D(0.5sG<1.5),
F(G<O.5).

6.3 Startup
This section introduces the operations related to the startup of the analyzer, including turning on the
instrument and launching the terminal software.

6.3.1 Start the analyzer

Before turning on the analyzer, make sure the autoloading area is cleared without racks or any other
objects.

1. Place the power switch at the left side of the analyzer in the [I] position.
The power indicator light will be on.
2. Check the indicator light on the analyzer.
If the indicator light is on, it indicates the analyzer has been started up.

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6.3.2 Log in Terminal Software

• Before running the software, make sure the network cable of the external computer is properly
connected to the analyzer. The analyzer starts to initialize only when the connection are
detected.
• If you failed to run the software continuously, please contact BIOTA customer service
department or your local agent immediately.
• After startup, please make sure the data/time of the computer is correct.
• You can either start up the main unit first or run the software first.

1. Start the external computer.


2. Turn on the display.

3. After entering the operation system, double click the icon to run the software.
After starting the software, the message box as shown in Figure 6-2 will pop up.
Figure 6·2 Login

4. Enter the correct user name and password in the Login message box.
The initial user name and password of administrator are admin, which was set by service
engineer.
1 to 12 digits of numeric characters can be entered for the user name and the password. No
Chinese character is allowed.
5. Click Login.
The system starts to execute the initialization operations.
The whole process lasts for 4 to 12 minutes. The time needed for initializing the system depends
on how the analyzer was shut down previously.
For the background Ref. Range of each parameter, please see A.5.2 Normal Background.

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• The background test is designed for detecting particle interference and electrical interference.
• The sample ID for the background test is background.
• If the background results exceed the Ref. Range for the first time during fluidics initialization,
then the analyzer will run the background test one more time.
• Running a test when there is a Background abnormal, you would obtain an unreliable testing
result.
• If any error is detected during initialization (e.g. the background results exceed the Ref. Range),
the analyzer will activate the alarm.

6.3.3 Log off/Switch User


You can refer to the following steps to log off or switch users.

1. Click on the top right corner of the screen.


The following dialog box will pop up.

2. Click OK and enter the username and password in the dialog box.

3. Click Login to log in the interface as a different user.

6.4 Daily Quality Control


To ensure reliable analysis results, conduct daily QC analysis on the analyzer before running
samples. See 10 Quality Control.

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6.5 Sample Collection and Handling

All the samples, controls, calibrators, reagents, wastes and areas in contact with them are potentially
biohazardous. Wear proper personal protective equipment (e.g. gloves, lab uniforms, etc.) and follow
laboratory safety procedures when handling them and the relevant areas in the laboratory.

Do not touch the patients' blood sample directly.

• Do not re-use such disposable product as collection tubes, test tubes, capillary tubes, etc.
• Prepare the samples as per the procedures recommended by the reagent manufacturer.

• Be sure to use clean K2EDTA vacutainer blood collection tubes with anticoagulant, fused silica
glass/plastic test tubes, centrifugal tubes and borosilicate glass capillary tubes.
• Be sure to use the BIOTA-specified disposable products including vacutainer blood collection
tube, vacutainer blood collection tubes with anticoagulant and capillary tubes etc.
• For the whole blood samples to be used for WBC classification or PLT count, store them at
room temperature and run them within 8 hours after collection.
• If you do not need the PLT, MCV and WBC differential results, you can store the samples in a
refrigerator (2°C - 8°C) for 24 hours. You need to warm the keep samples at room temperature
for at least 30 minutes before running them.
• Be sure to shake any sample that has been prepared for a while before running it.

6.5.1 Venous Whole Blood Samples


The procedure for preparing whole blood samples is as follows:
1. Use clean K2EDTA (1.5-2.2mg/mL) vacutainer blood collection tubes with anticoagulant to
collect venous blood samples.
2. Shake the sample well according to your laboratory's protocol.

For vacutainer blood collection tube ( 12X75, cap excluded), please make sure the volume of the
whole blood sample is not less than 0.5mL.

6.5.2 Capillary Whole Blood Samples


The procedure for preparing capillary whole blood sample is as follows:

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1. Users clean centrifugal tube with anticoagulant to collect capillary whole blood samples.
2. Mix the sample according to your laboratory's protocol.

To ensure the accuracy of the analysis, make sure the volume of the capillary whole blood sample is
not less than 100 L.

Run the capillary whole blood sample within 3 minutes to 2 hours after its collection.

6.5.3 Prediluted Samples


The procedure for preparing prediluted sample is as follows:
1. Click the Add Diluent button in the function button area.
The system will prompt you with a message to add the diluent.

2. Take a clean centrifugal tube, uncap and set it under the sample probe as shown in the following
picture so that the probe tip is vertically in contact with the bottom of the tube so as to avoid
bubbles, liquid attached to the inner wall or spatter.

3. Press the aspirate key and add the diluent (180 L at a time).
After the diluent is added and you hear a beep, you can remove the centrifugal tube.
4. Add 20 L of capillary blood to the diluent, close the tube cap and shake the tube to mix the
sample.

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5. After the prediluted sample is prepared, click Cancel to exit dispensing the diluent.
6. If more portions of diluent are needed, repeat steps 3-4.

• You can also dispense 180 L of diluent by pipette into the tube.
• Be sure to keep dust from the prepared diluent.
• Be sure to run the prediluted samples within 30 minutes after the mixing.
• Be sure to mix any sample that has been prepared for a while before running it.
• Be sure to evaluate predilute stability based on your laboratory's sample population and sample
collection techniques or methods.

6.6 Sample Analysis


The analyzer provides the following four blood sample modes and two measurement modes for
sample analysis.
Blood sample modes include:
• Open-vial Venous Whole Blood (VWB)
• Open-vial Capillary Whole Blood (CWB)
• Open-vial Predilute (PD)
• Auto Venous Whole Blood (AWB)
Measurement modes include:
• CBC
• CBC+DIFF
Where, the VWB, CWB and PD modes belong to open-vial sampling analysis, the AWB mode
belongs to autoloader sampling analysis. The sample analysis procedures for open-vial sampling
and autoloader sampling will be presented on the following pages.

6.6.1 Open·vial Sampling Analysis

All the samples, controls, calibrators, reagents, wastes and areas in contact with them are potentially
biohazardous. Wear proper personal protective equipment (e.g. gloves, lab uniforms, etc.) and follow
laboratory safety procedures when handling them and the relevant areas in the laboratory.

The sample probe tip is sharp and may contain biohazardous materials. Exercise caution to avoid
contact with the probe when working around it.

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• Do not re-use such disposable product as collection tubes, test tubes, capillary tubes, etc.
• Make sure that the entered sample ID and mode exactly match those of the samples to be run.

• During aspiration, the tip of the probe should be kept at a certain distance from the bottom of the
sample container, otherwise the accuracy of aspiration volume will be affected.
• Keep the tip of the probe from contacting with the wall of the test tube to avoid blood splashing.
• Proper reference range shall be selected on the Setup interface before analysis. Otherwise, the
results may be flagged erroneously.
• Open-vial sampling analysis includes VWB sample analysis, CWB sample analysis and PD
sample analysis. Since the analysis procedures are similar, they will be presented together.

The system does not run samples as per the worklist by default. If the Run as per the worklist is
checked (See 5.3.1 Auxiliary Settings), the sample analysis will be conducted according to the
worklist upon start-up.
The sample analysis procedures in the conditions mentioned above will be presented on the
following pages.

Do not run as per the worklist


If you are not running samples as per the worklist, do as follows:
1. When the green indicator light is steady-on, click Mode & 1D in the function button area to enter
the Mode & 1D interface.
2. Choose the blood sample mode Open-vial-Venous Whole Blood (VWB), Open-vial-Capillary
Whole Blood (CWB) or Open-vial-Predilute (PD) in the Mode selection area according to the
actual situation.
Figure 6-3 Open-vial sampling analysis (do not run as per the worklist)

3. Choose the measurement mode CBC or CBC+D1FF, and enter the Sample ID.

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Refer to Table 6-1 for the description of relevant parameters.


Table 6-1 Description of open-vial sampling analysis parameters
Parameter Meaning Operation

Open-vial- Running the venous whole blood


Venous Whole samples in open-vial sampling Select from the radio box.
Blood (VWB) mode.

Open-vial-Capill Running the capillary whole blood


ary Whole Blood samples in open-vial sampling Select from the radio box.
(CWB) mode.

Open-vial-Predil Running the prediluted samples in


Select from the radio box.
ute (PD) open-vial sampling mode.

Complete Blood Count with no


differential count for white blood
CBC cells. The counting results comprise Select from the radio box.
15 parameters and the histograms
for WBC, RBC and PLT.

Complete Blood Count plus


differential count for white blood
cells. In addition to a total of 25
CBC+DIFF parameters, differential scattergrams Select from the radio box.
and the histograms for WBC/BASO,
RBC and PLT, the counting results
also include 4 research parameters.

Input in the textbox directly.


NOTE
• Letters, numbers and all
characters that can be entered
through the keyboard (including
special characters) are allowed
for the Sample ID. Chinese and
Identification number for the other languages (such as
Sample ID Japanese, Korean, etc) are not
samples to be run.
supported.
• The length of the entries ranges
from 1 to 25 and the entries shall
not be empty.
• The last character of a sample ID
must be numeric, but a string of
"0" only is not an acceptable
sample ID.

4. Click OK.
5. Shake the capped tube of sample for a homogeneous specimen.
For details about the preparation of blood samples, see 6.5 Sample Collection and Handling.
6. Remove the tube cap carefully and place the sample under the probe so that the probe can
aspirate the well-mixed sample.
7. Click Start or press the aspirate key on the analyzer to start running the sample.
The sample will be automatically aspirated by the sample probe.

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When the predilute counting begins, the system will prompt a dialog box. To disable such
reminders, please refer to 5.3.1 Auxiliary Settings.

8. When you hear a beep, remove the sample tube.


The analyzer will automatically run the sample and the analysis status icon and analyzer
indicator is flickering in green; the information area of the Next Sample will be refreshed.
When the analysis is complete, the analysis status icon and analyzer indicator return to
constantly-on green.
9. Repeat steps 5-8 to run the remaining samples.

• When the analyzer is running the samples, you can switch to Report interface to perform
operations including data browsing, validating, sample information editing, printing, etc. (see 7
Report), and you can also switch to other interfaces.
• When the analyzer is running the samples, all the functions related to the fluidics sequence are
not available.

Run as per the worklist


If the samples are set to be run as per the worklist, the analyzer will obtain the sample information in
the worklist according to the entered sample ID, then perform the counting. In this case, you don't
need to set the blood sample mode and measurement mode of the sample.

For details about the setting of the Run as per the worklist, see 5.3.1 Auxiliary Settings.

The procedure for running samples as per the worklist is as follows:


1. Input the sample information in the Worklist interface.
For details, see 8 Worklist.
2. When the green indicator light is steady-on, click Mode & 1D in the function button area to enter
the Mode & 1D interface.

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Figure 6-4 Open-vial sampling analysis (run as per the worklist)

3. Enter the sample ID, which should be the same as the sample to be run in the worklist.
The analyzer will match the sample information in the worklist according to the sample ID.
) If a matched sample ID is found, the sample will be run according to the blood sample mode
and measurement mode in the worklist.
) If no matching sample ID is found, and it's the first running sample, then the sample will be
run in the mode as you set.
) If no matching sample ID is found, and it's not the first running sample, then the sample will
be run in the mode of the last run.
4. Click OK.
5. Shake the capped tube of sample for a homogeneous specimen.
For details about the preparation of blood samples, see 6.5 Sample Collection and Handling.
6. Remove the tube cap carefully and place the sample under the probe so that the probe can
aspirate the well-mixed sample.
7. Click Start or press the aspirate key on the analyzer to start running the sample.
The sample will be automatically aspirated by the sample probe.
8. When you hear a beep, remove the sample tube.
The analyzer will automatically run the sample and the analysis status icon and analyzer
indicator is flickering in green; the information area of the Next Sample will be refreshed.
When the analysis is complete, the analysis status icon and analyzer indicator return to
constantly-on green.
9. Repeat steps 5-8 to run the remaining samples.

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• When the analyzer is running the samples, you can switch to Report interface to perform
operations including data browsing, validating, sample information editing, printing, etc. (see 7
Report), and you can also switch to other interfaces.
• When the analyzer is running the samples, all the functions related to the fluidics sequence are
not available.

6.6.2 Autoloader Sampling Analysis

All the samples, controls, calibrators, reagents, wastes and areas in contact with them are potentially
biohazardous. Wear proper personal protective equipment (e.g. gloves, lab uniforms, etc.) and follow
laboratory safety procedures when handling them and the relevant areas in the laboratory.

• The sample probe tip is sharp and may contain biohazardous materials. Exercise
caution to avoid contact with the probe when working around it.
• Be sure to avoid reversing the collection tube when loading, otherwise, the collection tube may
be broken and/or cause biohazard.
• Collection tubes broken may cause personal injury and/or biohazard. Be sure to place the
collection tubes in the right adapter before running, otherwise, the collection tubes may be
broken and cause biohazard.
• Before starting autoloader sample analysis, keep your hands away from the racks to avoid injury.

• Do not re-use such disposable product as collection tubes, test tubes, capillary tubes, etc.
• Repeat piercing the vacutainer blood collection tube may break the rubber tube cap. The
fragments produced may lead to incorrect analysis result. It is recommended that do not pierce
each tube for more than three times.
• Sample clump may lead to incorrect analysis results. Check if clump exists before running the
samples; if it does, handle it as per the related laboratory procedures.
• Make sure that the entered sample ID, rack No., tube No. and mode could match those of the
sample to be run exactly.

• During aspiration, the tip of the probe should be kept at a certain distance from the bottom of the
sample container, otherwise the accuracy of aspiration volume will be affected.
• Keep the tip of the probe from contacting with the wall of the test tube to avoid blood splashing.

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• Proper reference range shall be selected on the Setup interface before analysis. Otherwise, the
results may be flagged erroneously.

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6.6.2.1 Preparation
1. Prepare tubes and racks as instructed by 6.2.2 Tube and Barcode Preparation.
2. Prepare whole blood samples as instructed by 6.5.1 Venous Whole Blood Samples, and place
them in the racks.
If tube barcode scanning is required, make sure the barcode labels on the tubes, which are
placed in the racks, are facing the analyzer.
3. Place racks from the outer side of loading tray.
Placing rack from the center of loading tray may easily lead to the rack out of level.
4. Check if there is a rack in the feeding area; if so, remove it manually.
5. Remove all rack(s) on the unloading tray.

6.6.2.2 Starting Sample Analysis

• During sample analysis, please do not move the rack or tubes in the feeding area.
• If more than 6 racks samples are needed to be analyzed, you should load the rack from the right
of the autoloader while removing the completed rack from the left of the autoloader in time. Load
the rack from the outer side of loading tray, and do not push forward it.
• Make sure that the entered sample ID, rack No., tube No. and mode could match those of the
sample to be run exactly.
• If any error happens during the analysis process, which leads to remaining racks in feeding area,
click Remove Error. The analyzer will remove the error automatically and unload the rack to the
left tray of the autoloader.
• In case of power failure during sample analysis, remove the rack manually. Then open the front
housing to check if there is a fallen tube; if so, take it out.

According to different settings, the autoloader sampling analysis can be divided into:
• Do not scan the sample ID or rack No. and do not run as per the worklist
• Do not scan the sample ID or rack No. but run as per the worklist
• Automatically scan the sample ID and/or rack No. but do not run as per the worklist
• Automatically scan the sample ID and/or rack No. and run as per the worklist

Do not scan the sample 1D or rack No. and do not run as per the worklist
When running autoloader sampling analysis, the analyzer does not scan the sample ID or rack No.
and does not run as per the worklist by default. Take the following steps to perform autoloader
sampling analysis.

• For details about the settings of the sample ID and rack No. scanning, see the description of
Automatically scan Sample 1D and Automatically scan Rack No. in 5.3.5 Autoloader.
• For details about the setting of the Run as per the worklist, see 5.3.1.1 Aspirating- Run as
per the worklist.
1. When the green indicator light is steady-on, click Mode & 1D in the function button area to enter
the Mode & 1D interface.

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2. Choose Auto-Venous Whole Blood (AWB) in the Mode selection.

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See Figure 6-5.


Figure 6·5 Running the AWB Samples

3. Select the measurement mode CBC or CBC+D1FF according to the actual situation, and enter
the values of Sample 1D, Rack No. and Tube No..
Refer to Table 6-2 for the description of relevant parameters.
Table 6·2 Description of AWB Sample analysis parameters

Parameter Meaning Operation

Auto-Venous
Running the venous whole blood
Whole Blood Select from the radio box.
samples in autoloader sampling mode.
(AWB)

Complete Blood Count with no


differential count for white blood cells.
CBC The counting results comprise 15 Select from the radio box.
parameters and the histograms for WBC,
RBC and PLT.

Complete Blood Count plus differential


count for white blood cells. In addition to
a total of 25 parameters, differential
CBC+DIFF scattergrams and the histograms for Select from the radio box.
WBC/BASO, RBC and PLT, the
counting results also include 4 research
parameters.

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Parameter Meaning Operation

Input in the textbox directly.


NOTE
• Letters, numbers and all
Starting identification number of the characters that can be entered
through the keyboard (including
sample to be run. The next sample ID special characters) are allowed
will increase sequentially. for the Sample ID. Chinese and
other languages (such as
Sample ID NOTE
Japanese, Korean, etc) are not
You don't need to input the sample ID if supported.
the Automatically scan Sample 1D is • The length of the entries ranges
checked. See section 5.3.5 from 1 to 25 and the entries shall
Autoloader. not be empty.
• The last character of a sample ID
must be numeric, but a string of
"0" only is not an acceptable
sample ID.

Starting rack number of the sample to be


run.
NOTE Input in the textbox directly. The
Rack No.
You don't need to input the rack No. if input range is 1 to 100.
the Automatically scan Rack No. is
checked. See section 5.3.5
Autoloader.

Starting tube number of the sample to be


run. Input in the textbox directly.
Tube No. For example, if you enter 5 in the The input range is 1 to 10.
textbox, the sample analysis will be
started from the fifth tube position of the
first rack.

4. Click OK to save the settings.


5. Place racks loading tubes in ascending order on the level of the right tray of the autoloader, with
the rack numbers on the racks facing the analyzer.
6. Click the Start button or press the [RUN] key on the analyzer to start the sample analysis from
the starting position as you set.

After starting the AWB sample analysis, the Start button will be replaced by Stop. When you click
Stop, the analysis will be stopped after the previously analyzing of the pierced sample is finished.
Then the statistical result will be displayed.

When the sample analysis is complete, a statistics dialog box will pop up as shown in Figure 6-6.

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Figure 6·6 AWB sample analysis statistic

Refer to Table 6-3 Description of AWB sample analysis statistics parameters for the description
of relevant parameters.
Table 6·3 Description of AWB sample analysis statistics parameters

Parameter Description

Rack No. and Tube No. of the sample.


Sample Position For example, 1-2 means the rack No. of the sample is 1, and the
tube No. is 2.

Identification number of the sample.


If the sample ID is displayed like INVALID1 (INVALID2,
INVALID3, .), it means the sample ID entered or scanned is invalid.
The reason may be:
Sample ID • Invalid Rack No.
• Invalid tube barcode (sample ID)
• Worklist query failed
• LIS query failed

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Parameter Description

Indicates that the analyzer skipped the


Skip (Not at starting sample without running because the
position) sample was placed before the starting
position.

Indicates that the analyzer skipped the


Skip (Invalid Rack No.) sample without running because Rack No.
scanning failed.

Indicates that the analyzer skipped the


Skip (Invalid tube
sample without running because Sample
barcode)
ID scanning failed.

Indicates that the analyzer skipped the


Skip (Worklist query sample without running because no
failed) matching record was found when running
as per the worklist.

Indicates that the analyzer skipped the


Remarks Skip (LIS query failed) sample without running because LIS
communication failed.

Indicates that the sample analysis is


Run
complete.

Indicates that the sample analysis is


Run (Invalid Rack No.)
complete with Rack No. scanning failure.

Run (Invalid tube Indicates that the sample analysis is


barcode) complete with Sample ID scanning failure.

Indicates that the sample analysis is


Run (Worklist query
complete, but no matching record is found
failed)
in the worklist.

Indicates that the sample analysis is


Run (LIS query failed)
complete with LIS communication failure.

Indicates that there is no tube in the


Vacancy
sample position.

Finished samples Number of analyzed samples.

Skipped samples Number of skipped samples without analyzing.

Rack vacancies Number of vacancies on the rack.

You can set whether or not to display the autoloading sampling analysis statistics, and whether
or not to run samples when scanning failed or any error happened. See section 5.3.5 Autoloader.

7. Click OK to close the message box.


The analysis status icon and analyzer indicator return to constantly-on green.
When finish running, all the racks come to the left tray of the autoloader. Remove them safely.

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6 Daily Operations

• When the analyzer is running the samples, you can switch to Report interface to perform
operations including data browsing, validating, sample information editing, printing, etc. (see 7
Report), and you can also switch to other interfaces.
• When the analyzer is running the samples, all the functions related to the fluidics sequence are
not available.
• After every analysis cycle, the results will be saved to the Review interface.

Do not scan the sample 1D or rack No. but run as per the worklist
If the analyzer does not scan the sample ID or rack No. but runs as per the worklist, you can take
the following steps to perform autoloader sampling analysis.

• For details about the settings of sample ID and rack No. scanning, see the description of
Automatically scan Sample 1D and Automatically scan Rack No. parameters in 5.3.5
Autoloader.
• For details about the setting of the Run as per the worklist, see 5.3.1.1 Aspirating- Run as
per the worklist.

1. When the green indicator light is steady-on, click Mode & 1D in the function button area to enter
the Mode & 1D interface.
2. Choose Auto·Venous Whole Blood (AWB) in the Mode selection.
See Figure 6-7.
Figure 6·7 Running the AWB Samples

3. Enter the rack No. and tube No., which should be the same as the sample to be run in the
worklist.
The analyzer will match the sample information in the worklist according to the sample position
(Rack No. and Tube No.), then perform the analysis.

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6 Daily Operations

) If a matched record is found, the analyzer will run the sample according to the mode as you

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6 Daily Operations

set in the worklist.


) If no matching record is found, and the Skip the sample when it failed to match the
worklist is checked, then the analyzer will skip the sample and continue with other samples.
) If no matching record is found, and the Skip the sample when it failed to match the
worklist is unchecked, then the analyzer will run the sample in CBC+DIFF mode.
Refer to Table 6-4 for the description of relevant parameters.
Table 6·4 Description of AWB Sample analysis parameters
Parameter Meaning Operation

Auto-Venous
Running the venous whole blood samples in
Whole Blood Select from the radio box.
autoloader sampling mode.
(AWB)

Complete Blood Count with no differential


count for white blood cells. The counting
CBC Select from the radio box.
results comprise 15 parameters and the
histograms for WBC, RBC and PLT.

Complete Blood Count plus differential count


for white blood cells. In addition to a total of 25
parameters, differential scattergrams and the
CBC+DIFF Select from the radio box.
histograms for WBC/BASO, RBC and PLT,
the counting results also include 4 research
parameters.

Starting rack number of the sample to be run.


NOTE Input in the textbox directly.
Rack No.
You don't need to input the rack No. if the The input range is 1 to 100.
Automatically scan Rack No. is checked.
See section 5.3.5 Autoloader.

Starting tube number of the sample to be run.


For example, if you enter 5 in the textbox, the Input in the textbox directly.
Tube No.
sample analysis will be started from the fifth The input range is 1 to 10.
tube position of the first rack.

4. Click OK.
5. Place racks loading tubes in ascending order on the level of the right tray of the autoloader, with
the rack numbers on the racks facing the analyzer.
6. Click the Start button or press the [RUN] key on the analyzer to start the sample analysis from
the starting position as you set.

After starting the AWB sample analysis, the Start button will be replaced by Stop. When you click
Stop, the analysis will be stopped after the previously analyzing of the pierced sample is finished.
Then the statistical result will be displayed.

When the sample analysis is complete, a statistics dialog box will pop up as shown in Figure 6-8.

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6 Daily Operations

Figure 6·8 AWB sample analysis statistics

Refer to Table 6-5 for the description of relevant parameters.


Table 6·5 Description of AWB sample analysis statistics parameters

Parameter Description

Rack No. and Tube No. of the sample.


Sample Position For example, 1-2 means the rack No. of the sample is 1, and the tube
No. is 2.

Identification number of the sample.


If the sample ID is displayed like INVALID1 (INVALID2, INVALID3, .), it
means the sample ID entered or scanned is invalid. The reason may be:
Sample ID • Invalid Rack No.
• Invalid tube barcode (sample ID)
• Worklist query failed
• LIS query failed

Indicates that the analyzer skipped the


Skip (Not at starting
Remarks sample without running because the sample
position)
was placed before the starting position.

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Parameter Description

Indicates that the analyzer skipped the


Skip (Invalid Rack No.) sample without running because Rack No.
scanning failed.

Indicates that the analyzer skipped the


Skip (Invalid tube
sample without running because Sample ID
barcode)
scanning failed.

Indicates that the analyzer skipped the


Skip (Worklist query sample without running because no matching
failed) record was found when running as per the
worklist.

Indicates that the analyzer skipped the


Skip (LIS query failed) sample without running because LIS
communication failed.

Indicates that the sample analysis is


Run
complete.

Indicates that the sample analysis is complete


Run (Invalid Rack No.)
with Rack No. scanning failure.

Run (Invalid tube Indicates that the sample analysis is complete


barcode) with Sample ID scanning failure.

Indicates that the sample analysis is


Run (Worklist query
complete, but no matching record is found in
failed)
the worklist.

Indicates that the sample analysis is complete


Run (LIS query failed)
with LIS communication failure.

Indicates that there is no tube in the sample


Vacancy
position.

Finished samples Number of analyzed samples.

Skipped samples Number of skipped samples without analyzing.

Rack vacancies Number of vacancies on the rack.

You can set whether or not to display the autoloading sampling analysis statistics, and whether or
not to run samples when scanning failed or any error happened. See section 5.3.5 Autoloader.

7. Click OK to close the message box.


The analysis status icon and analyzer indicator return to constantly-on green.
When finish running, all the racks come to the left tray of the autoloader. Remove them safely.

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6 Daily Operations

• When the analyzer is running the samples, you can switch to Report interface to perform
operations including data browsing, validating, sample information editing, printing, etc. (see 7
Report), and you can also switch to other interfaces.
• When the analyzer is running the samples, all the functions related to the fluidics sequence are
not available.
• After every analysis cycle, the results will be saved to the Review interface.

Automatically scan the sample 1D and/or rack No. but do not run as per the worklist
If the analyzer is set to automatically scan the sample ID and/or rack No. but not run as per the
worklist, you can take the following steps to perform autoloader sampling analysis.

• For details about the settings of the Sample ID and rack No. scanning, see the description of
Automatically scan Sample 1D and Automatically scan Rack No. in 5.3.5 Autoloader.
• For details about the setting of the Run as per the worklist, see 5.3.1.1 Aspirating- Run as
per the worklist.
• When placing tubes in the rack, make sure the barcode labels on the tubes are facing the
analyzer.

1. When the green indicator light is steady-on, click Mode & 1D in the function button area to enter
the Mode & 1D interface.
2. Choose Auto·Venous Whole Blood (AWB) in the Mode selection.
See Figure 6-9.
Figure 6·9 Running the AWB Samples

3. Choose the measurement mode CBC or CBC+D1FF.


4. Enter the values of Sample 1D, Rack No. and Tube No. according to the actual situation.
) If the Automatically scan Rack No. and Automatically scan Sample 1D are displayed, the
starting tube No. should be entered.

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) If the Automatically scan Rack No. is displayed, the starting sample ID and starting tube No.

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6 Daily Operations

should be entered.
) If the Automatically scan Sample 1D is displayed, the starting rack No. and starting tube No.
should be entered.

• If the barcode scan is successful, the sample analysis will be performed in the set mode.
• The analyzer will skip the sample and continue with other samples when: the sample ID
scanning failed and the parameter Skip the sample when Sample 1D scanning failed is
checked, or the rack No. scanning failed and the parameter Skip the sample when Rack
No. scanning failed is checked.
• The analyzer will run the sample in CBC+DIFF mode when: the sample ID scanning failed
and the parameter Skip the sample when Sample 1D scanning failed is unchecked, or
the rack No. scanning failed and the parameter Skip the sample when Rack No.
scanning failed is unchecked.
• For details about the settings of Skip the sample when Sample 1D scanning failed and
Skip the sample when Rack No. scanning failed parameters, see 5.3.5 Autoloader.

Refer to Table 6-6 for the description of relevant parameters.


Table 6·6 Description of AWB Sample analysis parameters

Parameter Meaning Operation

Auto-Venous Running the venous whole blood


Whole Blood samples in autoloader sampling Select from the radio box.
(AWB) mode.

Complete Blood Count with no


differential count for white blood
CBC cells. The counting results comprise Select from the radio box.
15 parameters and the histograms
for WBC, RBC and PLT.

Complete Blood Count plus


differential count for white blood
cells. In addition to a total of 25
CBC+DIFF parameters, differential scattergrams Select from the radio box.
and the histograms for WBC/BASO,
RBC and PLT, the counting results
also include 4 research parameters.

Input in the textbox directly.


NOTE
Starting identification number of the
• Letters, numbers and all characters that
sample to be run. The next sample
can be entered through the keyboard
ID will increase sequentially. (including special characters) are
NOTE allowed for the Sample ID. Chinese and
Sample ID other languages (such as Japanese,
You don't need to input the sample Korean, etc) are not supported.
ID if the Automatically scan • The length of the entries ranges from 1
Sample 1D is checked. See to 25 and the entries shall not be empty.
section
5.3.5 Autoloader. • The last character of a sample ID must
be numeric, but a string of "0" only is not
an acceptable sample ID.

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6 Daily Operations

Parameter Meaning Operation

Starting rack number of the sample


to be run.
NOTE Input in the textbox directly. The input
Rack No.
You don't need to input the rack No. range is 1 to 100.
if the Automatically scan Rack
No. is checked. See section 5.3.5
Autoloader.

Starting tube number of the sample


to be run.
For example, if you enter 5 in the Input in the textbox directly.
Tube No.
textbox, the sample analysis will be The input range is 1 to 10.
started from the fifth tube position of
the first rack.

5. Click OK to save the settings.


6. Place racks loading tubes in ascending order on the level of the right tray of the autoloader, with
the rack numbers on the racks facing the analyzer.
7. Click the Start button or press the [RUN] key on the analyzer to start the sample analysis from
the starting position as you set.

After starting the AWB sample analysis, the Start button will be replaced by Stop. When you click
Stop, the analysis will be stopped after the previously analyzing of the pierced sample is finished.
Then the statistical result will be displayed.

When the sample analysis is complete, a statistics message box will pop up. See Figure 6-10.

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6 Daily Operations

Figure 6·10 AWB sample analysis statistics

Refer to Table 6-7 for the description of relevant parameters.


Table 6·7 Description of AWB sample analysis statistics parameters

Parameter Description

Rack No. and Tube No. of the sample.


Sample Position For example, 1-2 means the rack No. of the sample is 1, and the
tube No. is 2.

Identification number of the sample.


If the sample ID is displayed like INVALID1 (INVALID2,
INVALID3, .), it means the sample ID entered or scanned is invalid.
The reason may be:
Sample ID • Invalid Rack No.
• Invalid tube barcode (sample ID)
• Worklist query failed
• LIS query failed

Indicates that the analyzer skipped the


Skip (Not at starting sample without running because the
Remarks
position) sample was placed before the starting
position.

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Parameter Description

Indicates that the analyzer skipped the


Skip (Invalid Rack No.) sample without running because Rack No.
scanning failed.

Indicates that the analyzer skipped the


Skip (Invalid tube
sample without running because Sample
barcode)
ID scanning failed.

Indicates that the analyzer skipped the


Skip (Worklist query sample without running because no
failed) matching record was found when running
as per the worklist.

Indicates that the analyzer skipped the


Skip (LIS query failed) sample without running because LIS
communication failed.

Indicates that the sample analysis is


Run
complete.

Indicates that the sample analysis is


Run (Invalid Rack No.)
complete with Rack No. scanning failure.

Run (Invalid tube Indicates that the sample analysis is


barcode) complete with Sample ID scanning failure.

Indicates that the sample analysis is


Run (Worklist query
complete, but no matching record is found
failed)
in the worklist.

Indicates that the sample analysis is


Run (LIS query failed)
complete with LIS communication failure.

Indicates that there is no tube in the


Vacancy
sample position.

Finished samples Number of analyzed samples.

Skipped samples Number of skipped samples without analyzing.

Rack vacancies Number of vacancies on the rack.

You can set whether or not to display the autoloading sampling analysis statistics, and whether or
not to run samples when scanning failed or any error happened. See section 5.3.5 Autoloader.

8. Click OK to close the message box.


The analysis status icon and analyzer indicator return to constantly-on green.
When finish running, all the racks come to the left tray of the autoloader. Remove them safely.

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6 Daily Operations

• When the analyzer is running the samples, you can switch to Report interface to perform
operations including data browsing, validating, sample information editing, printing, etc. (see 7
Report), and you can also switch to other interfaces.
• When the analyzer is running the samples, all the functions related to the fluidics sequence are
not available.
• After every analysis cycle, the results will be saved to the Review interface.

Automatically scan the sample 1D and/or rack No. and run as per the worklist
If the analyzer is set to automatically scan the sample ID and/or rack No. and run as per the worklist,
you can take the following steps to perform autoloader sampling analysis.

• For details about the settings of the sample ID and rack No. scanning, see the description of
Automatically scan Sample 1D and Automatically scan Rack No. in 5.3.5 Autoloader.
• For details about the setting of the Run as per the worklist, see 5.3.1.1 Aspirating- Run as
per the worklist.
• When placing tubes in the rack, make sure the barcode labels on the tubes are facing the
analyzer.

1. When the green indicator light is steady-on, click Mode & 1D in the function button area to enter
the Mode & 1D interface.
2. Choose Auto·Venous Whole Blood (AWB) in the Mode selection.
See Figure 6-11.
Figure 6·11 Running the AWB Samples

3. Enter the starting tube No. of the sample to be run.


) If the Run as per the worklist and Automatically scan Sample 1D are displayed (no matter
whether the Automatically scan Rack No. is displayed or not), the analyzer will obtain the
sample information in the worklist according to the scanned sample ID.

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) If the Run as per the worklist and Automatically scan Rack No. are displayed, the

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analyzer will obtain the sample information in the worklist according to the sample position.

• If the barcode scanning and worklist matching are successful, the analyzer will run the
sample in the mode as you set in the worklist, and update the sample position.
• The analyzer will skip the sample and continue with other samples when: no matching
record is found and the Skip the sample when it failed to match the worklist is checked;
the sample ID scanning failed and the parameter Skip the sample when Sample 1D
scanning failed is checked; or the rack No. scanning failed and the parameter Skip the
sample when Rack No. scanning failed is checked.
• The analyzer will run the sample in CBC+DIFF mode when: no matching record is found,
and the Skip the sample when it failed to match the worklist is unchecked; the sample
ID scanning failed and the parameter Skip the sample when Sample 1D scanning
failed is unchecked; or the rack No. scanning failed and the parameter Skip the sample
when Rack No. scanning failed is unchecked.
• For details about the settings of Skip the sample when it failed to match the worklist,
Skip the sample when Sample 1D scanning failed and Skip the sample when Rack
No. scanning failed, see 5.3.5 Autoloader.

Refer to Table 6-8 for the description of relevant parameters.


Table 6·8 Description of AWB Sample analysis parameters
Parameter Meaning Operation

Auto-Venous
Running the venous whole blood samples in
Whole Blood Select from the radio box.
autoloader sampling mode.
(AWB)

Complete Blood Count with no differential


count for white blood cells. The counting
CBC Select from the radio box.
results comprise 15 parameters and the
histograms for WBC, RBC and PLT.

Complete Blood Count plus differential count


for white blood cells. In addition to a total of
25 parameters, differential scattergrams and
CBC+DIFF Select from the radio box.
the histograms for WBC/BASO, RBC and
PLT, the counting results also include 4
research parameters.

Starting rack number of the sample to be run.


NOTE Input in the textbox directly.
Rack No.
You don't need to input the rack No. if the The input range is 1 to 100.
Automatically scan Rack No. is checked.
See section 5.3.5 Autoloader.

Starting tube number of the sample to be run.


For example, if you enter 5 in the textbox, the Input in the textbox directly.
Tube No.
sample analysis will be started from the fifth The input range is 1 to 10.
tube position of the first rack.

4. Click OK to save the settings.

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5. Place racks loading tubes in ascending order on the level of the right tray of the autoloader, with
the rack numbers on the racks facing the analyzer.

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6. Click the Start button or press the [RUN] key on the analyzer to start the sample analysis from
the starting position as you set.

After starting the AWB sample analysis, the Start button will be replaced by Stop. When you click
Stop, the analysis will be stopped after the previously analyzing of the pierced sample is finished.
Then the statistical result will be displayed.

When the sample analysis is complete, a statistics dialog box will pop up as shown in Figure
6-12.
Figure 6·12 AWB sample analysis statistics

Refer to Table 6-9 for the description of relevant parameters.


Table 6·9 Description of AWB sample analysis statistics parameters

Parameter Description

Rack No. and Tube No. of the sample.


Sample Position For example, 1-2 means the rack No. of the sample is 1, and the
tube No. is 2.

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Parameter Description

Identification number of the sample.


If the sample ID is displayed like INVALID1 (INVALID2,
INVALID3, .), it means the sample ID entered or scanned is invalid.
The reason may be:
Sample ID • Invalid Rack No.
• Invalid tube barcode (sample ID)
• Worklist query failed
• LIS query failed

Indicates that the analyzer skipped the


Skip (Not at starting sample without running because the
position) sample was placed before the starting
position.

Indicates that the analyzer skipped the


Skip (Invalid Rack No.) sample without running because Rack No.
scanning failed.

Indicates that the analyzer skipped the


Skip (Invalid tube
sample without running because Sample
barcode)
ID scanning failed.

Indicates that the analyzer skipped the


Skip (Worklist query sample without running because no
failed) matching record was found when running
as per the worklist.

Indicates that the analyzer skipped the


Remarks Skip (LIS query failed) sample without running because LIS
communication failed.

Indicates that the sample analysis is


Run
complete.

Indicates that the sample analysis is


Run (Invalid Rack No.)
complete with Rack No. scanning failure.

Run (Invalid tube Indicates that the sample analysis is


barcode) complete with Sample ID scanning failure.

Indicates that the sample analysis is


Run (Worklist query
complete, but no matching record is found
failed)
in the worklist.

Indicates that the sample analysis is


Run (LIS query failed)
complete with LIS communication failure.

Indicates that there is no tube in the


Vacancy
sample position.

Finished samples Number of analyzed samples.

Skipped samples Number of skipped samples without analyzing.

Rack vacancies Number of vacancies on the rack.

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You can set whether or not to display the autoloading sampling analysis statistics, and whether or
not to run samples when scanning failed or any error happened. See section 5.3.5 Autoloader.

7. Click OK to close the message box.


The analysis status icon and analyzer indicator return to constantly-on green.
When finish running, all the racks come to the left tray of the autoloader. Remove them safely.

• When the analyzer is running the samples, you can switch to Report interface to perform
operations including data browsing, validating, sample information editing, printing, etc. (see 7
Report), and you can also switch to other interfaces.
• When the analyzer is running the samples, all the functions related to the fluidics sequence are
not available.
• After every analysis cycle, the results will be saved to the Review interface.

6.6.2.3 Stopping Sample Analysis


When the analyzer is running AWB samples, the Start button will be replaced by Stop. When you
click Stop, the analysis will be stopped after the previously analyzing of the pierced sample is
finished.

6.6.2.4 1nserting STAT


If there is STAT sample requires running first during AWB sample analysis, you can click the STAT
button in the function button area to start the STAT sample analysis.
The autoloader will be stopped after the previously analyzing of the pierced sample is finished.
Then, the Mode & 1D, Add Diluent, Start and Exit STAT buttons in the function button area will be
activated. See Figure 6-13.
Figure 6·13 1nserting STAT

You can run the STAT sample in ways as the open-vial sampling mode, see details in 6.6.1
Open-vial Sampling Analysis.

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During STAT sample analysis, you don't need to move the racks on the autoloader which was
stopped. After exiting STAT, the autoloading sampling analysis will continue.

6.6.2.5 Exiting STAT


After the STAT sample analysis is finished, click the Exit STAT button to cancel the STAT operation.
The analysis mode will be switched to before the inserted STAT. Then the analyzer will automatically
run the unfinished samples.

6.6.3 Dealing with the Analysis Results

6.6.3.1 Automatic saving of analysis results


This analyzer automatically saves sample results. When the maximum number has been reached,
the newest result will overwrite the oldest (already backed up). The maximum number of the
automatically saved results is 300,000.

6.6.3.2 Parameter Flags


• If parameter is followed by a "↑" or "↓", it means the analysis result has exceeded the upper or
lower limit of the reference range but still within the display range.
• If the parameter is followed by a "?", it means the analysis result is suspicious.
• If you see *** instead of a result, it means the result is either invalid or beyond the display range.

For the background test, the flags for parameters or abnormal blood cell differential and morphology
are not available.

6.6.3.3 Flags of Abnormal Blood Cell Differential or Morphology


The analyzer will flag abnormal or suspicious WBC, RBC and PLT according to the scattergrams
and histograms. The flag information is defined in the table below.
Table 6·10 Flags of abnormal blood cell differential or morphology

Flag Type Flag information

Leucocytosis

Leucopenia

Neutrophilia
WBC Abnormal
Neutropenia

Lymphocytosis

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Lymphopenia

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Flag Type Flag information

Monocytosis

Eosinophilia

Basophilia

WBC abnormal

Abn.WBC scattergram

Abn. WBC histogram

Left Shift?

Suspicious Immature Cell?

RBC Lyse Resistant?

Abnor./Atypical Lym?

Abnormal WBC Channel

Abnormal DIFF Channel

Erythrocytosis

Anisocytosis

Macrocytosis
Abnormal
Microcytosis

Anemia

Hypochromia
RBC/HGB
Abnor. RBC Distr.

Dimorphologic

Iron Deficiency?

Suspicious HGB Abn./Interfere?

RBC Clump?

Abnormal RBC Channel

Abnormal HGB Channel

Thrombocytosis
Abnormal
Thrombopenia
PLT
Abnor. PLT Distr.
Suspicious
PLT Clump?

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The system shows flags for abnormal or suspicious items in different samples and measurement
modes in accordance with the impact of the abnormal or suspicious WBC, RBC or PLT items on the
results of the parameters. The correlation is shown in the following table.
Table 6·11 Flags for abnormal or suspicious items in different samples and measurement modes

Whole Blood Predilute


Type Flag
CBC CBC+DIFF CBC CBC+DIFF

WBC abnormal √ √ √ √

RBC Lyse Resistant? x √ x √

Abnormal WBC scattergram x √ x √

Abnormal WBC histogram √ √ √ √

Left Shift? x √ x √

Immature Granulocyte (IG)? x √ x √

Abnormal/Atypical Lymphocyte? x √ x √

Leucocytosis √ √ √ √

Leucopenia √ √ √ √
WBC
Neutrophilia x √ x √

Neutropenia x √ x √

Lymphocytosis x √ x √

Lymphopenia x √ x √

Monocytosis x √ x √

Eosinophilia x √ x √

Basophilia x √ x √

Abnormal WBC Channel √ √ √ √

Abnormal DIFF Channel x √ x √

Dimorphologic √ √ √ √

HGB Abn/Interfere? √ √ √ √

Anisocytosis √ √ √ √

Microcytosis √ √ √ √

RBC/HGB Macrocytosis √ √ √ √

Erythrocytosis √ √ √ √

Anemia √ √ √ √

Hypochromia √ √ √ √

Abn. RBC distr. √ √ √ √

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Whole Blood Predilute


Type Flag
CBC CBC+DIFF CBC CBC+DIFF

Iron Deficiency? � � � �

RBC Clump? � � � �

Abnormal RBC Channel � � � �

Abnormal HGB Channel � � � �

PLT Clump? � � � �

Thrombocytosis � � � �
PLT
Thrombopenia � � � �

Abnor. PLT distr. � � � �

• "� " indicates that flags will be displayed in the mode. "x" indicates that flags will not be displayed
in the mode.
9
• When the PLT value is less than 1OOx1O /L, a manual count by the microscope
is recommended.

6.7 Report Management


Upon the completion of sample analysis, you can process the results in the Report interface and
print the report.
For more details on the report, please refer to 7 Report.

6.8 Shutdown

All the samples, controls, calibrators, reagents, wastes and areas in contact with them are potentially
biohazardous. Wear proper personal protective equipment (e.g. gloves, lab uniforms, etc.) and follow
laboratory safety procedures when handling them and the relevant areas in the laboratory.

The sample probe is sharp and potentially biohazardous, Exercise caution to avoid contact with the
probe when working around it.

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Do not turn on the analyzer immediately after its shutdown. Wait at least 10 seconds before
power-on to avoid damage to the machine.

• To ensure stable analyzer performance and accurate analysis results, be sure to perform the
Shutdown procedure to shut down the analyzer after it has been running continuously for
24 hours.
• If the analyzer is shut down abnormally, you will lose the unsaved worklist information of the
samples.
• Be sure to shut down the analyzer in strict accordance with the instruction below.

Shutdown here refers to the shutdown of the analyzer and the external computer. The following
sections will introduce both procedures.

6.8.1 Shutting down the analyzer


Procedures for shutting down the analyzer are as follows:

1. Click on the top right corner of the screen.


The following message box will pop up.

2. Click Yes.
The system starts to execute the shutdown sequence and a message box pops up showing the
procedures for cleanser maintenance as below.

3. Follow the instructions and set the cleanser under the sample probe, and press the aspirate key
on the analyzer or click Aspirate to run the first cleanser sample aspiration.

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Upon the completion of the first sample aspiration, a message box will pop up as below.

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4. Follow the instructions and set the cleanser under the sample probe again, press the aspirate
key on the analyzer or click Aspirate to run the second cleanser sample aspiration.
Upon the completion of cleanser maintenance, a dialog box will pop up as below.

5. Turn to [O] the [O1I] switch located on the left side of the analyzer.
Once the analyzer is powered off, the following dialog box will pop up.

6. Click Yes and the software program will automatically shut down.
If you click No, the software program will not exit and you are still able to perform any operation
independent from the analyzer.
7. After the shut-down, vacate the waste containers and handle the waste properly.

Be sure to dispose reagents, waste, samples, consumables, etc. according to local legislations and
regulations.

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• When the analyzer is not connected to the computer, shutdowns will not be executed.
• When the analyzer is running or performing other fluidics sequence, do not force shutdown the
analyzer.
• If any error is detected during shutdown procedure, the analyzer will return to the status before
the shutdown procedure is performed, and then activate the alarm. See
14 Troubleshooting for details of removing the error.

6.8.2 Turning off the external computer

You should exit the terminal software first and then turn off the external computer according to
standard procedures. Otherwise, the database of the terminal software might be lost!

1. Turn off the external computer according to the shutdown procedures of the operation system.
2. Turn off the display.

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