Analyte Quarterly Vol1 2019 Ms

Analyte
Quarterly
Vol. 1 2019
Look closer at your assays,
platforms and results in
your multiplex and single
protein detection assays
• MILLIPLEX® map Assays

• SMC™ (Single Molecule
Counting) Assays
• ELISA
• RIA
• Custom Assay Development
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Platforms Fit for Your Purpose
PROTEIN DETECTION PLATFORMS
We’re here to guide you to choose the best protein detection

platform for your needs
What’s better than a high quality, sensitive, reproducible, robust immunoassay that fits your
study requirements? Having a scientific partner offering you a variety of high performance assay
platforms. Whether you need rich profiling data from a multiplex panel using Luminex® technology,
ultrasensitive detection from an SMC™ assay, or robust ELISAs we help you get data you trust.
MILLIPLEX® map Multiplex Detection

• Widest range of research areas—and species
• Broadest selection of analytes from which to choose
SMC™ Assays for SMCxPRO™ or Erenna® Instruments

• Previously undetectable assays, now quantified
• Measure to the femtogram/mL level
ELISAs
• >1,800 ELISAs, EIAs and RIAs against numerous soluble circulating and intracellular biomarkers
Flexibility and sensitivity: our platforms fit your purpose

Protein Custom
Detection Sample Dynamic Multiplex Assay
Platforms Fit for Purpose Quantitative Sensitivity Volume Range Capability Support
Luminex® platform
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Multiplex detection
Yes pg/mL ≤ 25 µL ••• Yes
Flexible platform
SMCxPRO™ or Erenna® system
Ultrasensitivity
Yes fg/mL 5–100 µL ••• Yes
High performance
ELISA
Plate reader compatibility

Yes pg/mL 50–100 µL •• Yes
Most widely cited
• Good Performance •• Strong Performance ••• Superior Performance Not Recommended Recommended
2 | sigmaaldrich.com/immunoassays
PROTEIN DETECTION PLATFORMS
NEW IMMUNOASSAY PRODUCTS
NEW! MILLIPLEX® map and Single Molecule Counting (SMC™) Kits
NEW KITS
Description Species Cat. No.
MILLIPLEX® map Panels
Cytokine Panel 1 Bovine BCYT1-33K
Immuno-Oncology Checkpoint Protein Panel 1 (expanded) Human HCKP1-11K
Pituitary Panel Canine CANPIT-96K
SMC™ Immunoassay Kits
IL-1α High Sensitivity Immunoassay Kit Human 03-0167-00
IL-5 High Sensitivity Immunoassay Kit Human 03-0168-00
IL-17A/F High Sensitivity Immunoassay Kit Human 03-0177-00
SMC™ Immunogenicity Bead Based Assay Development Kit --- 03-0175-00
VEGF-A High Sensitivity Immunoassay Kit Human 03-0180-00
Coming Soon
Description Species Cat. No.
MILLIPLEX® map Panels
Aging Mouse Coming Soon!
Metabolic Mouse Coming Soon!
Cytokine Autoantibody Human Coming Soon!
SMC™ Streamlined Kits
SMC™ Bead Based Assay Development Kit (5 plates) -- Coming Soon!
SMC™ Plate Based Assay Development Kit (5 plates) -- Coming Soon!
IFN-γ High Sensitivity Kit Human Coming Soon!
New SMC™ Streamlined Kit Improvements:

Making it easier for you to do your work
• Improved options for sample prep and incubation

• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Increased reagent volumes to accommodate automation
• Kits are verified to work on both SMCxPRO™ and Erenna® instruments
sigmaaldrich.com/immunoassays | 3
MILLIPLEX® map Analyte Index
MILLIPLEX® map ANALYTE INDEX
Analyte Page Analyte Page Analyte Page
4–6 BAX (total)........................................18 Complement Factor B ........................10

4E-BP1 (total) ...................................19 BCA-1/CXCL13 ................................... 9 Complement Factor H .................. 10, 12
4E-BP1 (Thr37/46) ............................19 Bcl-2 (total).......................................18 Complement Factor I .........................10
5’-NT/CD73 ................................ 12, 16 Bcl-2 (Ser70) ....................................18 Connective Tissue Growth Factor
6Ckine/CCL21/Exodus-2 ................ 9, 13 Bcl-xL (total).....................................18 (CTGF)..............................................15
Bcl-xL/BAD (interaction).....................18 Contactin-1 ......................................12
A BDNF................................10, 12, 14, 15 Corticosterone ..................................15
α 1-Acid Glycoprotein (AGP) Betacellulin.......................................14 Cortisol ............10, 11, 12, 13, 14, 15, 16
.......................................11, 14, 15, 16 BIM .................................................18 Creatine Kinase Muscle (CKM) ............15
α-1-Antitrypsin (A1AT) .......................12 Blk (Tyr389) .....................................19 CREB (Ser133) ....................... 18, 19, 20
MILLIPLEX® map
α-1 Microglobulin...............................12 BMP-9...............................................12 CREB (total) ............................... 18, 20

α-2 Macroglobulin (A2M).....11, 12, 14, 15 BNP..................................................11 CTACK/CCL27 ............................... 9, 11
α-Fetoprotein (AFP) ...........................11 BRAK/CXCL14.................................... 9 CTLA-4/CD152...................................11
α-MSH .................................. 12, 15, 16 BTLA................................................11 Cardiac Troponin I (cTnI)......... 11, 14, 15
α-Synuclein ......................................12 Cardiac Troponin T (cTnT)......... 11, 14, 15
ACTH ......................... 11, 13, 14, 15, 16 C CXCL16 ...................................9, 11, 14
Active Caspase 3...............................21 c-Jun (Ser73) ....................................18 CYFRA21-1 .......................................11
Active Caspase 8 (total).....................18 c-Kit (panTyr) ...................................18 Cystatin C ............................. 12, 15, 16
Active Caspase 9 (total).....................18 c-Kit/SCFR (soluble)...........................12
ADAM15 ...........................................11 c-Met/HGFR (panTyr) .........................18 D
ADAMTS13 .......................................11 c-Myc (total)......................................19 D-dimer ...........................................11
Adiponectin ........................... 10, 14, 15 C-Peptide.................................... 10, 13 DKK1 ...............................11, 12, 14, 16
Adipsin/Factor D .................... 10, 11, 14 C-Peptide 2................................. 14, 15 dPAPP-A ...........................................11
Agouti-Related Protein (AgRP) ...... 11, 13 C-Reactive Protein (CRP)......... 11, 12, 14 DPP4 ...............................................11
ALK-1 (soluble)..................................14 CA125..............................................11
Akt/PKB (Ser473) .............. 17, 18, 19, 21 CA15-3.............................................11 E
Akt/PKB (total) ................. 17, 18, 19, 21 CA19-9 ............................................11 EGF ............................. 9, 12, 14, 15, 16
Akt1 (Ser473) ...................................19 Cadherin 13 (CDH13)..........................11 EGFR/HER1/ErbB1 (soluble).......... 10, 12
Akt1 (total) ......................................19 Calbindin..........................................12 EGFR2/Her2/ErbB2 (soluble)...............12
Akt2 (Ser474) ...................................19 Carbonic Anhydrase 9 (CA9)................12 EGFR3/Her3/ErbB3 (soluble)...............12
Akt2 (total) ......................................19 Cathepsin D......................................12 eIF-4B (Ser422) ................................19
Akt3 (Ser472) ...................................19 Caveolin-1 (CAV-1).............................15 eIF-4E (Ser209) ................................19
Akt3 (total) ......................................19 CCL28............................................... 9 eIF-4G (Ser1108) ..............................19
Albumin...................................... 12, 16 CD3ε (panTyr) ..................................19 eIF2a (Ser51) ...................................19
ALDH1A1..........................................12 CD14 (soluble)...................................11 ENA-78/CXCL5 ................................... 9
Amphiregulin.....................................14 CD27................................................11 Endocan-1 (ESM-1) ...................... 11, 14
Amylin (active)..................10, 13, 14, 15 CD28................................................11 Endoglin .................................... 12, 14
Amylin (total).............................. 10, 16 CD30 (soluble)............................ 10, 14 Endostatin .......................................11
Amyloid beta 1-40 (Aβ 1-40) ........ 12, 15 CD31/PECAM-1 (soluble)......... 11, 12, 14 Endothelin-1 ............................... 12, 14
Amyloid beta 1-42 (Aβ 1-42) ........ 12, 15 CD40 ...............................................11 Eotaxin-2/CCL24/MPIF-2 ..................... 9
Angiogenin (ANG)..............................12 CD40L (soluble).......................9, 13, 14 Eotaxin-3/CCL26 ................................ 9
Angiopoietin-2............................. 12, 14 CD44 ...............................................12 Eotaxin/CCL11 .........................9, 13, 16

Angiostatin/Kringle............................12 CD80/B7-1 .......................................11 EpCAM .............................................12
Angiotensinogen (AGT).......................12 CD86/B7-2 .......................................11 Erythropoietin (EPO) .........10, 13, 14, 15
ANGPTL3..........................................11 CD137/4-1BB/TNFRSF9 (soluble)..........13 ErbB1/EGFR (panTyr) .........................18
ANGPTL4..........................................11 CD163 (soluble)................................11 ErbB2/HER2 (panTyr) ........................18
ANGPTL6/AGF....................................11 CEA..................................................11 ErbB3/HER3 (panTyr) ........................18
Antithrombin III................................11 CENP-A (Centromere Protein A) ErbB4/HER4 (panTyr) ........................18
Apelin...............................................10 (autoantibody)...................................10 Erk/MAPK 1/2 (Thr185/Tyr187)
Apolipoprotein AI (Apo AI) .................11 CENP-B (Centromere Protein B) .......................................18, 19, 20, 21
Apolipoprotein AII (Apo AII)................11 (autoantibody)...................................10 Erk/MAPK 1/2 (total) ..............18, 20, 21
Apolipoprotein B (Apo B) ....................11 Chk1 (Ser345) ..................................18 E-Selectin (soluble)............11, 12, 14, 15
Apolipoprotein CII (Apo CII) ...............11 Chk2 (Thr68) ....................................18 Estradiol .................... 11, 13, 14, 15, 16
Apolipoprotein CIII (Apo CIII) ...... 11, 12 Chromogranin-A (CHGA/CGA) ............11 Extracellular Matrix Protein 1 (ECM1)
Apolipoprotein E (Apo E) .............. 11, 12 CK-MB .............................................11 .......................................................11
ApoE4...............................................12 Cleaved PARP (total) .........................21
ARG1.......................................... 12, 16 Clusterin ............................... 12, 15, 16 F
ATF2 (Thr71) ....................................18 CNTF ......................................... 11, 13 FABP1 ........................................ 11, 12
ATR (total)........................................18 Collagen IV ......................................12 FABP3 ..............................10, 11, 12, 15
AXL (soluble).....................................12 Complement C1q (autoantibody)..........10 FABP4 ..............................................11
Complement C1q ..............................10 FADD (Ser194) ..................................19
B Complement C2 ................................10 Fas Ligand (sFasL) (soluble)
β-2-Microglobulin.................... 12, 15, 16 Complement C3 .......................... 10, 12 ................................... 9, 10, 11, 13, 14
β-Endorphin........................... 12, 15, 16 Complement C3b / iC3b .....................10 Fas/TNFRSF6 (soluble)... 9, 10, 11, 13, 16
β2-Glycoprotein (autoantibody)...........10 Complement C4 ................................10 Ferritin ............................................12
β -Tubulin (total) ...............................21 Complement C4b ..............................10 Fetuin A ..................................... 11, 12
BAD (total)........................................18 Complement C5 ................................10 FGF-1/FGF-acidic ..............................12
BAD (Ser112) ............................. 18, 21 Complement C5a ..............................10 FGF-19 .............................................11
BAFF/Blys ......................................... 9 Complement C9 ................................10 FGF-2/FGF-basic ................................ 9
Human pp. 9-12; Primate p. 13; Mouse pp. 13-15; Rat pp. 15-16;
4 | sigmaaldrich.com/milliplex Other Species p. 16; Cell Signaling pp. 17-21; Custom p. 23
MILLIPLEX® map Analyte Index (continued)
FGF-21 .............................10, 11, 14, 15 IFNβ ................................................. 9 IL-31 ........................................... 9, 13

FGF-23 ................................. 11, 14, 16 IFNβ1...............................................13 IL-32α ............................................... 9
FGFR1 (panTyr) .................................18 IFNγ ................................. 9, 13, 15, 16 IL-33/NF-HEV (mature) .................. 9, 13
Fgr (Tyr412) .....................................19 IgA ............................................ 10, 14 IL-34 ................................................ 9
Fibrinogen .................................. 11, 15 IgE ............................................ 10, 14 IL-35 ................................................ 9
Fibroblast Activation Protein (FAP).......12 IGF-1 ...............................................11 IL-36β /IL-1F8 .................................... 9
Fibronectin .......................................10 IGF1R ..............................................11 IL-36RA/IL-1F5..................................16
Flt3 Ligand.................................... 9, 16 IGF-2 ...............................................11 IL-37/IL-1F7 ....................................... 9
Flt3 (panTyr) ....................................18 IGF1R (total) ....................................17 IL-38/IL-1F10 ..................................... 9
Follistatin (FST)...................... 11, 12, 14 IGF1R (panTyr) .................................18 Insulin .............................................10
MILLIPLEX® map
Follistatin-like Protein 1 (FSTL1).... 14, 15 IGF1R (Tyr1135/1136) .......................17 Involucrin ........................................10
Fractalkine/CX3CL1 ............................ 9 IGFBP1 ............................................11 IP-10/CXCL10 ............... 9, 12, 13, 15, 16
FSH ........................... 11, 13, 14, 15, 16 IGFBP2 ............................................11 IR (total)...........................................17
Fyn (Tyr420) .....................................19 IGFBP3 ...................................... 11, 12 IR (panTyr) .......................................18
IGFBP4 ............................................11 IR (Tyr1162/1163) .............................17
G IGFBP5 ............................................11 Irisin .................................... 10, 14, 15
G-CSF ............................................... 9 IGFBP6 ............................................11 IRS1 (Ser636) .............................17, 20
Galectin-3.........................................12 IGFBP7 ............................................11 IRS1 (total) .................................17, 20
GAPDH (total) ...................................21 IgG1 .......................................... 10, 14 I-TAC/CXCL11 .................................... 9
GDF-11.............................................11 IgG2 ................................................10
GDF-15....................................... 11, 12 IgG2a ..............................................14 J
GDNF ...............................................12 IgG2b ..............................................14 Jag1.................................................11
GH ............................ 11, 13, 14, 15, 16 IgG3 .......................................... 10, 14 JNK/SAPK1 (Thr183/Tyr185)....18, 20, 21
Ghrelin (active) ........... 10, 13, 14, 15, 16 IgG4 ................................................10 JNK/SAPK1 (total) ..................18, 20, 21
GIP (total) .................. 10, 13, 14, 15, 16 IgM ........................................... 10, 14 Jo-1 (autoantibody)............................10
GITR ...............................................11 IκBα (Ser32) .....................................19
GITRL ..............................................11 IKKα/β (Ser177/181) ..........................19 K
Glial Fibrillary Acidic Protein (GFAP) ....12 IL-1α ............................ 9, 10, 13, 15, 16 Kallikrein-6 ......................................12
GLP-1 (active) ............. 10, 13, 14, 15, 16 IL-1β ................. 9, 10, 11, 13, 14, 15, 16 KC-like Protein...................................16
GLP-1 (total) ....................................10 IL-1RI (soluble)............................ 10, 14 KC/GROα /CXCL1 ..............................13
Glucagon ................... 10, 13, 14, 15, 16 IL-1RII (soluble)........................... 10, 14 Keratin-1, 10.....................................10
GM-CSF ............................ 9, 13, 15, 16 IL-1Ra .....................................9, 13, 16 Keratin-6 .........................................10
GnRH...............................................11 IL-2 .................................. 9, 13, 15, 16 KIM-1 ................................... 12, 15, 16
GOT1 ...............................................16 IL-2Rα (soluble)........................... 10, 14 Ku (autoantibody)..............................10
GP130 (soluble)........................... 10, 14 IL-3 ............................................. 9, 13
Granzyme A ................................. 9, 13 IL-4 .................................. 9, 13, 15, 16 L
Granzyme B ............................9, 10, 13 IL-4R (soluble)............................. 10, 14 Lactotransferrin (LTF)........................10
GRO ............................................ 9, 16 IL-5 .................................. 9, 13, 15, 16 LAG-3 ..............................................11
GROα/KC/CINC-1/CXCL1 ....................15 IL-6 .................. 9, 10, 11, 13, 14, 15, 16 LAT (panTyr) .....................................19
GSK3α (total)....................................17 IL-6R (soluble)............................. 10, 14 Lck (panTyr) .....................................19
GSK3α (Ser21) ..................................17 IL-6Rα (soluble).................................12 Lck (Tyr394) .....................................19

GSK3β (total)....................................17 IL-7 ........................................9, 13, 16 LDLR ...............................................11
GSK3β (Ser9) ...................................17 IL-8/CXCL8 ........................................ 9 Leptin ..............10, 11, 12, 13, 14, 15, 16
GSTα ......................................... 12, 16 IL-9 ............................................. 9, 13 Leptin Receptor (LEPR)/OB-R..............11
IL-10 ............................ 9, 11, 13, 15, 16 Leucine-rich alpha-2-glycoprotein 1
H IL-11 ............................................ 9, 13 (LRG1)..............................................11
H2A.X (Ser139) .................................18 IL-12 ................................................16 Leukemia Inhibitory Factor (LIF)
Haptoglobin ........................... 11, 14, 15 IL-12 (p40) ..............................9, 13, 16 ................................... 9, 10, 13, 14, 15
HB-EGF ............................................12 IL-12 (p70) ........................ 9, 13, 15, 16 LH ...................................11, 13, 14, 15
HCC-1/CCL14 ..................................... 9 IL-12/23 (p40) ..................................13 LIGHT ........................................ 11, 14
HCC-4/CCL16 .................................... 9 IL-13 ................................. 9, 13, 15, 16 LIX ............................................ 13, 15
HCGβ ...............................................11 IL-14/α -Taxilin ................................... 9 LIX/CXCL6/GCP-2.......................... 9, 11
Hck (Tyr411) .....................................19 IL-15 ............................ 9, 10, 13, 14, 15 L-Selectin (soluble)............................11
HE4 .................................................11 IL-16 ............................................ 9, 13 Lymphotactin/XCL1 ............................ 9
Hepsin .............................................12 IL-17A/CTLA8 ............... 9, 13, 14, 15, 16 Lyn (Tyr397) .....................................19
HGF .................................10, 11, 12, 14 IL-17A/F ...........................................13
HGFR/c-Met (soluble).........................12 IL-17E/IL-25 ................................. 9, 13 M
HMGB1 ............................................. 9 IL-17F .......................................... 9, 13 M-CSF ......................................... 9, 13
HSP27 (Ser78) ..................................18 IL-18 ............................ 9, 11, 13, 15, 16 Mannose-Binding Lectin (MBL)............10
HSP60 .............................................11 IL-19 ................................................. 9 MCAM/MUC18/sCD146........................11
HSP70 .............................................10 IL-20 ........................................... 9, 13 Mcl-1/BIM (interaction).......................18
Human Serum Albumin (HSA) .............10 IL-21 ........................................... 9, 13 Mcl-1 (total)......................................18
HVEM ..............................................11 IL-22 ........................................... 9, 13 MCP-1/CCL2 ...................................... 9
IL-23 ........................................... 9, 13 MCP-2/CCL8 ...................................... 9
I IL-24 ................................................ 9 MCP-3/CCL7 ...................................... 9
I-309/CCL1 ........................................ 9 IL-27 ........................................... 9, 13 MCP-4/CCL13 .................................... 9
ICAM-1 (soluble).......... 10, 11, 12, 14, 15 IL-28A/IFNλ2 ................................ 9, 13 MCP-5/CCL12 ...................................13
ICOS ...............................................11 IL-28B/IFNλ3 ................................ 9, 13 MSCFR (panTyr) ................................18
IFNα2 ............................................... 9 IL-29/IFNλ1 ....................................... 9 MDC/CCL22 .................................. 9, 13
Other Species p. 16; Cell Signaling pp. 17-21; Custom p. 23 sigmaaldrich.com/milliplex | 5
MDH1 ..............................................12 p38/SAPK2A/B (Thr180/Tyr182) Sclerostin (SOST) .................. 11, 14, 16

MDM2 (total).....................................18 ............................................18, 20, 21 SDF-1/CXCL12 .............................. 9, 14
MEK1 (Ser222) .................................18 p38/SAPK2A/B (total) .............18, 20, 21 SDH .......................................... 12, 16
Melanoma Inhibitory Activity Protein p53 (Ser15) ......................................18 Serum Amyloid A (SAA) .....................11
(MIA) ...............................................12 p53 (Ser46) ......................................18 Serum Amyloid P (SAP) ........... 11, 12, 14
Melatonin ................................... 12, 15 p70S6 Kinase (total)......................17, 18 Sex Hormone Binding Globulin
Mesothelin .......................................12 p70S6 Kinase (Thr389/412) ...........17, 18 (SHBG) ............................................12
Mi-2 (autoantibody)............................10 PAI-1 (total) .....................10, 12, 14, 15 Sm (autoantibody).............................10
Midkine ............................................12 Pancreatic Polypeptide (PP) SMAD2 (Ser465/467) .........................19
MIF ........................................... 10, 11 ................................. 10, 13, 14, 15, 16 SMAD3 (Ser423/425) .........................19
MILLIPLEX® map
MIG/CXCL9 ................................... 9, 13 PARK5/UCHL1...................................12 SMAD4 (total)...................................19

MIP-1α/CCL3 ..................................... 9 PARK7/DJ1 Src (Tyr419) .....................................19
MIP-1β/CCL4 ..................... 9, 10, 13, 16 (Parkinson’s Disease Protein 7) ..........12 SSA/Ro52 (Sjögren’s Syndrome-related
MIP-1δ/MIP-5/CCL15 .......................... 9 PCNA (Proliferating Cell Nuclear antigen A/Ro52 kDa) (autoantibody)....10
MIP-2/CXCL2 .............................. 13, 15 Antigen A) (autoantibody)...................10 SSA/Ro60 (Sjögren’s Syndrome-related
MIP-3α/CCL20 .............................. 9, 13 PD-1 ................................................11 antigen A/Ro60 kDa) (autoantibody)....10
MIP-3β/CCL19 ............................... 9, 13 PDGF-AA ...................................... 9, 12 SB/La (Sjögren’s Syndrome-related
MIP-4/PARC/CCL18 ....................... 9, 12 PDGF-AB/BB ................................. 9, 12 antigen B/La) (autoantibody)...............10
MMP-1 .............................................10 PDGF-BB ..........................................16 ST2/IL1RL1 (soluble)..........................11
MMP-2 ....................................... 10, 14 PDGFRα (panTyr) ..............................18 STAT1 (total) ....................................21
MMP-3 ....................................... 10, 14 PDGFRβ (panTyr) ..............................18 STAT1 (Tyr701) ...................... 18, 19, 21
MMP-7 .............................................10 PD-L1 ..............................................11 STAT2 (Tyr690) ................................19
MMP-8 ....................................... 10, 14 PD-L2...............................................11 STAT3 (total) .............................. 18, 20
MMP-9 .............................................10 PEDF ...............................................12 STAT3 (Ser727) .................................18
MMP-10 ............................................10 Pentraxin-3 (PTX3) ............................11 STAT3 (Tyr705) ........................... 19, 20
MMP-12 ...................................... 10, 14 Perforin ....................................... 9, 13 STAT5A/B (total)................................19
MMP-13 ............................................10 Periostin ..........................................12 STAT5A/B (Tyr694/699) ............... 18, 19
MPIF/CCL23 ....................................... 9 Progranulin (pGRN)............................11 STAT6 (Tyr641) .................................19
MR-ProADM ......................................11 PL-12 (Alanyl-tRNA Synthetase) Substance P .......................... 12, 15, 16
MSK1 (Ser212) .................................18 (autoantibody)...................................10 Superoxide Dismutase 1 (soluble)
mTOR (Ser2448) .................... 17, 20, 21 PLA2G7 ............................................11 (sSOD1)............................................12
mTOR (total) ......................... 17, 20, 21 Placental Growth Factor 2 (PLGF-2) .....14 Superoxide Dismutase 2 (soluble)
Myeloperoxidase (MPO) ............... 11, 12 Placental Growth Factor (PLGF)..... 11, 12 (sSOD2)............................................12
Myeloperoxidase (MPO) Platelet Factor (PF4)/CXCL4 ...............11 Syk (panTyr) .....................................19
(autoantibody)...................................10 PM/Sci 100 (autoantibody)..................10 Syndecan 4 (SYND4) .........................11
Myoglobin ........................................11 PRNP (Prion Protein)...........................12
Myosin Light Chain 3 (MYL3) ..............15 Progesterone .............. 11, 13, 14, 15, 16 T
Myostatin/GDF8 ..................... 10, 14, 15 Prolactin ..........................11, 14, 15, 16 T3 ............................. 11, 13, 14, 15, 16
ProMMP-9 ........................................14 T4 ............................. 11, 13, 14, 15, 16
N Properdin/Factor P ............................10 TARC/CCL17 ................................. 9, 13
NAP-2/CXCL7 ..................................... 9 Proteinase 3 (autoantibody)................10 Tau (Total) .......................................12
NCAM (soluble)..................................12 PSA (free) ........................................11 Tau (Thr181) ....................................12

NCAML1/L1CAM/CD171 ......................12 PSA (total) .......................................11 Tenascin-C (TN-C).............................12
Neurogranin (NRGN)..........................12 P-Selectin (soluble)...................... 11, 14 Testosterone .............. 11, 13, 14, 15, 16
Neuron-specific Enolase (NSE) ...........12 PTEN (total)......................................17 TFF-3 ......................................... 12, 16
Neuropilin-1 (soluble).........................12 PTEN (Ser380) ..................................17 TGFα ......................................9, 11, 13
Neurotensin .......................... 12, 15, 16 PTGDS .............................................11 TGFβ1 ........................ 10, 13, 14, 15, 16
Neutrophil Elastase-2 (ELA2) ..............10 PTH ...................................... 11, 12, 16 TGFβ2 ........................ 10, 13, 14, 15, 16
NFκB (total)......................................18 PYY (total) ................. 10, 13, 14, 15, 16 TGFβ3 ........................ 10, 13, 14, 15, 16
NFκB (Ser536) ............................ 18, 19 TGFβRII (total)..................................19
NGAL/Lipocalin-2 ........ 10, 11, 12, 15, 16 R Thrombomodulin ......................... 11, 14
NGF .................................................10 RAGE (soluble)....................... 10, 12, 14 Thrombospondin-1 (TSP-1) ................10
Notch1 .............................................11 RANKL .............................................11 Thrombospondin-2 (TSP-2).................12
NOXA/Mcl-1 (interaction)....................18 RANTES/CCL5 .............. 9, 12, 13, 15, 16 TIE1 (panTyr) ...................................18
NT proBNP .......................................11 RBP4 ......................................... 12, 16 TIE2 (panTyr) ...................................18
Renin ......................................... 12, 15 Tie-2 (soluble)...................................12
O Resistin ..................................... 10, 14 TIM-3 ..............................................11
Oncostatin-M (OSM) ............... 10, 11, 14 Ribosomal P (autoantibody)................10 TIMP-1 ....................... 10, 12, 13, 15, 16
Osteoactivin .....................................12 RNP (Ribonucleoprotein) TIMP-2 .............................................10
Osteocalcin (OC) ...............................11 (autoantibody)...................................10 TIMP-3 .............................................10
Osteocrin (OSTN)/Musclin ....... 10, 14, 15 RNP/Smith (RNP/Sm) (autoantibody)....10 TIMP-4 ............................................10
Osteonectin/SPARC ................ 10, 12, 14 RPS6 (Ser235/Ser236) .......................17 Tissue Factor (TF) .............................11
Osteopontin (OPN) ............11, 12, 15, 16 RPS6 (total)......................................17 TLR-2 ..............................................11
Osteoprotegerin (OPG) ......11, 12, 14, 16 TNFR1 (total)....................................19
Oxytocin ............................... 12, 15, 16 S TNFα ................ 9, 10, 11, 13, 14, 15, 16
S100B ..............................................12 TNFβ/Lymphotoxin-α (LTA).............. 9, 13
P SCF ........................................9, 11, 16 TNF RI (soluble) (sTNFR1)............. 10, 14
p21 (total).........................................18 Scl-70 (autoantibody).........................10 TNF RII (soluble) (sTNFR2)............ 10, 14
TPO .................................................. 9 U VEGFR1/Flt-1 (soluble)............. 10, 12, 14

TRAIL/TNFSF10 ............................ 9, 11 ucMGP .............................................11 VEGFR2/KDR/Flk-1 (panTyr) ...............18
Transglutaminase 2 (TGM2)................12 uPAR (soluble)/CD87..........................12 VEGFR2/KDR/Flk-1 (soluble)..... 10, 12, 14
TRAP5 .............................................12 Uromodulin ......................................12 VEGFR3/Flt-4 (panTyr) .......................18
TREM2..............................................12 VEGFR3/Flt-4 (soluble)............ 10, 12, 14
Troponin I (TnI)...................... 11, 14, 15 V Vitamin D binding protein ..................11
Troponin T (TnT)..................... 11, 14, 15 VCAM-1 (soluble).................... 10, 11, 12
TSC2 (total)......................................17 VEGF ...............................................15 Y
TSC2 (Ser939) ..................................17 VEGF-A ............. 9, 11, 12, 13, 14, 15, 16 Yes (Tyr421) .....................................19
TSH ........................... 11, 13, 14, 15, 16 VEGF-C ...................................... 12, 14 YKL40/CHI3L1 .............................. 9, 12
MILLIPLEX® map
TSLP ................................................. 9 VEGF-D ...................................... 12, 14
TWEAK ............................................12 VEGFR1/Flt-1 (panTyr) .......................18 Z
ZAP-70 (panTyr) ................................19
Other Species p. 16; Cell Signaling pp. 17-21; Custom p. 23

What’s in the MILLIPLEX® kit?
Protocol Included
Detection Antibody Magnetic Beads
Cocktail Capture antibody-conjugated
beads in solution
10X Wash Buffer
96-well Plate
Greiner Black Mylar
Assay Buffer
Standard
Serum Matrix
Controls
Plate Sealers Bead Diluent QC1 & QC2
SAPE
Streptavidin Phycoerythrin
sigmaaldrich.com/milliplex | 7
MILLIPLEX® map Kit Index
MILLIPLEX® map KIT INDEX
Cat. No. Page Cat. No. Page Cat. No. Page Cat. No. Page
4 CGTMAG-98K....................16 HKI6MAG-99K...................12 MKI2MAG-94K..................15

46-602MAG......................21 CKT1MAG-97K..................16 HLINJMAG-75K..................12 MMHMAG-44K...................14
46-604MAG......................21 CKT2MAG-97K..................16 HLPPMAG-57K...................11 MMMP1MAG-79K...............14
46-609MAG......................21 HMHEMAG-34K.................10 MMMP2MAG-79K...............14
46-610MAG......................21 E HMMP1MAG-55K...............10 MMMP3MAG-79K...............14
46-612MAG......................21 EQCYTMAG-93K................16 HMMP2MAG-55K...............10 MMYOMAG-74K.................14
46-613MAG......................21 EQCYTMG-93KPX23...........16 HMYOMAG-56K.................10 MPTMAG-49K....................14
46-618MAG......................21 HNABTMAG-68K................12 MSCRMAG-42K..................14
MILLIPLEX® map
46-654MAG......................21 F HNCSMAG-35K..................12 MSHMAG-21K

46-655MAG......................21 FCYTMAG-20K-PMX............16 HND3MAG-39K..................12 ....................11,13,14,15,16
46-656MAG......................21 HNDG1MAG-36K...............12 MT17MAG47K-PX25...........13
46-667MAG......................21 H HNDG2MAG-36K...............12 MTH17MAG-47K................13
46-675MAG......................21 HADCYMAG-61K................10 HNDG3MAG-36K...............12
46-677MAG......................21 HADK1MAG-61K................10 HNDG4MAG-36K...............12 N
46-685MAG......................21 HADK2MAG-61K................10 HNPMAG-35K....................12 NHPMHMAG-45K...............13
46-686MAG......................21 HAGE1MAG-20K................11 HNS1MAG-95K..................12 NHPPT1MG-46K.................13
46-694MAG......................21 HAGP1MAG-12K................12 HNS2MAG-95K..................12
46-713MAG......................21 HAIAB-10K.......................10 HPRTKMAG-01K................18 P
48-602MAG......................21 HANG2MAG-12K................12 HPTP1MAG-66K.................11 PCYTMAG-23K...................16
48-610MAG......................19 HBNMAG-51K....................11 HRNKLMAG-51K-01............11 PCYTMG-23K-13PX............16
48-611MAG......................17 HCCB4MAG-58K................12 HSCRMAG-32K..................10 PCYTMG-40K-PX23............13
48-612MAG......................17 HCCBP1MAG-58K..............11 HSCRMAG32KPX14............10 PRCYT2MAG40K................13
48-614MAG......................19 HCCBP2MAG-58K..............11 HSP1MAG-63K..................10 PRCYTMAG40K-PX24..........13
48-618MAG......................19 HCCBP3MAG-58K..............12 HSP2MAG-63K..................10 PRCYTMAG40K-PX25..........13
48-619MAG......................20 HCD8MAG-15K................... 9 HSP3MAG-63K..................10 PRCYTOMAG-40K...............13
48-621MAG......................18 HCD8MAG15K17PMX........... 9 HSTC384-28K.................... 9
48-622MAG......................20 HCKP1-11K.......................11 HSTCMAG-28SK................. 9 R
48-623MAG......................20 HCKP1-11K-PX17...............11 HSTCMAG28SPMX13........... 9 RADPCMAG-82K................15
48-624MAG......................20 HCMBMAG-22K.................12 HSTCMAG28SPMX21........... 9 RADPKMAG-80K................15
48-625MAG......................20 HCMP1MAG-19K................10 HSTCMAG384-PX21............ 9 RBN1MAG-31K..................16
48-626MAG......................20 HCMP2MAG-19K................10 HT17MG-14K-PX25............. 9 RCI1MAG-87K...................15
48-628MAG......................20 HCP2MAG-62K-PX23........... 9 HTH17MAG-14K................. 9 RECYMAG65K27PMX..........15
48-630MAG......................19 HCVD1MAG-67K................11 HTMP1MAG-54K................10 RECYTMAG-65K.................15
48-631MAG......................19 HCVD2MAG-67K................11 HTMP2MAG-54K................10 RKTX1MAG-37K................16

48-632MAG......................19 HCVD3MAG-67K................11 RKTX2MAG-37K................16
48-633MAG......................19 HCVD4MAG-67K................11 M RLI1MAG-92K...................16
48-650MAG......................19 HCVD5MAG-67K................11 MABMAG-83K...................15 RMHMAG-84K...................15
48-655MAG......................19 HCVD6MAG-67K................11 MADCYMAG-72K................14 RMNPMAG-83K..................15
48-660MAG......................18 HCYP2MAG-62K................. 9 MADKMAG-71K.................14 RMYOMAG-88K..................15
48-669MAG......................18 HCYP3MAG-63K................. 9 MADPNMAG-70K-01...........14 RPTMAG-86K....................15
48-680MAG......................18 HCYP4MAG-64K................. 9 MAGPMAG-24K..................14 RSHMAG-69K....................15
48-681MAG......................18 HCYTMAG-60K-PX29........... 9 MAP2MAG-76K..................14 RTHYMAG-30K..................15
48-682MAG......................18 HCYTMAG-60K-PX30........... 9 MBNMAG-41K...................14 RV1MAG-26K....................15
48-683MAG......................18 HCYTMAG-60K-PX38........... 9 MCD8MAG-48K.................13 RV2MAG-26K....................15
48-690MAG......................19 HCYTMAG-60K-PX41........... 9 MCD8MAG48K-PX15...........13 RV3MAG-26K....................15
HCYTOMAG-60K................. 9 MCVD1MAG-77K................14
A HDIAB-34K-PMX5..............10 MCVD2MAG-77K................14 S
APOMAG-62K....................11 HGAMMAG-301K...............10 MCYTMAG-70K-PX32..........13 SKINMAG-50K...................10
HGAMMAG-303E................10 MCYTOMAG-70K................13
B HIGFBMAG-53K.................11 MCYTOMAG-70K-PMX.........13 T
BCYT1-33K.......................16 HIGFMAG-52K...................11 MECY2MAG-73K................13 TGFBMAG-64K-01
BCYT1-33K-PX15...............16 HIL18MAG-66K.................. 9 MECY2MAG-73KPX.............13 ....................10,13,14,15,16
HKI1MAG-99K...................12 MGAMMAG-300E...............14 TGFBMAG-64K-03
C HKI2MAG-99K...................12 MGAMMAG-300K...............14 ....................10,13,14,15,16
CANPIT-96K......................16 HKI3MAG-99K...................12 MHSTCMAG-70K................13
CCYTMG-90K-PX13............16 HKI4MAG-99K...................12 MHSTCMAG-70KPMX..........13
CCYTOMAG-90K................16 HKI5MAG-99K...................12 MKI1MAG-94K..................15
MILLIPLEX® map IMMUNOLOGY
HUMAN Human Cytokine/Chemokine
Panel III
384-well Human High
Sensitivity T Cell
(Cat. No. HCYP3MAG-63K) (Cat. No. HSTC384-28K)
Immunology 21 (Cat No. HSTCMAG384-PX21)
HCC-1 / CCL14 ▲ M-CSF
21 (Bulk Cat. No. HSTCMAG384PX21BK)
Human Cytokine/Chemokine IL-11 MIG / CXCL9
Panel I IL-29 / IFNλ1 MIP-3α / CCL20 Fractalkine / CX3CL1 IL-12 (p70)
(Cat. No. HCYTOMAG-60K) I-TAC / CXCL11 MIP-3β / CCL19 GM-CSF IL-13
29 (Cat. No. HCYTMAG-60K-PX29) LIX / CXCL6 / GCP-2 NAP-2 / CXCL7 ▲ IFNγ IL-17A / CTLA8
29 (Bulk Cat. No. HCYTMAG60PMX29BK) Lymphotactin / XCL1 IL-1β IL-21
30 (Cat. No. HCYTMAG-60K-PX30) ♦ IL-2 IL-23
30 (Bulk Cat. No. HCYTMAG60PMX30BK) ♦ IL-4 I-TAC / CXCL11
38 (Cat. No. HCYTMAG-60K-PX38)
Human Cytokine/Chemokine IL-5 MIP-1α / CCL3
38 (Bulk Cat. No. HCYTMAG60PMX38BK) Panel IV IL-6 MIP-1β / CCL4
41 (Cat. No. HCYTMAG-60K-PX41) (Cat. No. HCYP4MAG-64K) IL-7 MIP-3α / CCL20
41 (Bulk Cat. No. HCYTMAG60PMX41BK) IL-8 / CXCL8 TNFα
BAFF / Blys IL-28B / IFNλ3 IL-10
sCD40L IL-9 BRAK / CXCL14 IL-32α
EGF ♦ IL-10 ♦ CCL28 IL-34
Eotaxin / CCL11 ♦ IL-12 (p40) ♦ CXCL16 IL-35 Human Th17
FGF-2 / FGF-basic IL-12 (p70) ♦ HCC-4 / CCL16 IL-36β / IL-1F8
(Cat. No. HTH17MAG-14K)
Flt3 Ligand IL-13 ♦ HMGB1 ● IL-37 / IL-1F7
25 (Cat. No. HT17MG-14K-PX25)
Fractalkine / CX3CL1 IL-15 ♦ IFNβ IL-38 / IL-1F10
25 (Bulk Cat. No. HT17MAG14PMX25BK)
G-CSF ♦ IL-17A / CTLA8 ♦ IL-14 / α-Taxilin MIP-4 / PARC / CCL18
GM-CSF ♦ IP-10 / CXCL10 ♦ IL-19 MPIF / CCL23 GM-CSF IL-17E / IL-25
GRO MCP-1 / CCL2 ♦ IL-24 YKL40/CHI3L1 IFNγ IL-17F
IFNα2 ♦ MCP-3 / CCL7 IL-1β IL-21
IFNγ ♦ MDC / CCL22 IL-2 IL-22
HUMAN
IL-1α ♦ MIP-1α / CCL3 ♦ Human IL-18 Singleplex IL-4 IL-23
IL-1β ♦ MIP-1β / CCL4 ♦ (Cat. No. HIL18MAG-66K) IL-5 IL-27
IL-1Ra ♦ PDGF-AA ▲ IL-6 IL-28A / IFNλ2
IL-2 ♦ PDGF-AB / BB ▲ IL-18 IL-9 IL-31
IL-3 ♦ RANTES / CCL5 ♦▲ IL-10 IL-33 / NF-HEV
IL-4 ♦ TGFα IL-12 (p70) (mature)
IL-5 ♦ TNFα ♦ Human High Sensitivity IL-13 MIP-3α / CCL20
IL-6 ♦ TNFβ / Lymphotoxin-α T Cell IL-15 TNFα
IL-7 ♦ (LTA) ♦ IL-17A / CTLA8 TNFβ/Lymphotoxin-α
(Cat. No. HSTCMAG-28SK)
VEGF-A ♦ (LTA)
IL-8 / CXCL8 ♦ 13 (Cat. No. HSTCMAG28SPMX13) ♦
13 (Bulk Cat. No. HSTCMAG28PMX13BK) ♦
21 (Cat. No. HSTCMAG28SPMX21) Human CD8+ T Cell
Human Cytokine/Chemokine 21 (Bulk Cat. No. HSTCMAG28PMX21BK)
Panel II (Cat. No. HCD8MAG-15K)
Fractalkine / CX3CL1 IL-12 (p70) ♦ 17 (Cat. No. HCD8MAG15K17PMX)
(Cat. No. HCYP2MAG-62K) 17 (Bulk Cat. No. HCD8MAG15KPX17BK)
GM-CSF ♦ IL-13 ♦
23 (Cat. No. HCP2MAG-62K-PX23)
IFNγ ♦ IL-17A / CTLA8
23 (Bulk Cat. No. HCP2MAG62KPX23BK) sCD137/4-1BB/ IL-4
IL-1β ♦ IL-21 TNFRSF9 IL-5

6Ckine/CCL21/ IL-28A/IFNλ2 IL-2 ♦ IL-23 sFAS / TNFRSF6 IL-6
Exodus-2 IL-33/NF-HEV IL-4 ♦ I-TAC / CXCL11 sFasL IL-10
BCA-1/CXCL13 (mature) IL-5 ♦ MIP-1α / CCL3 GM-CSF IL-13
CTACK/CCL27 LIF IL-6 ♦ MIP-1β / CCL4 Granzyme A MIP-1α / CCL3
ENA-78/CXCL5 MCP-2/CCL8 IL-7 ♦ MIP-3α / CCL20 Granzyme B MIP-1β / CCL4
Eotaxin-2/CCL24/ MCP-4/CCL13 IL-8 / CXCL8 ♦ TNFα ♦ IFNγ Perforin
MPIF-2 MIP-1δ/MIP-5/CCL15 IL-10 ♦ IL-2 TNFα
Eotaxin-3/CCL26 SCF
I-309/CCL1 SDF-1/CXCL12
IL-16 TARC/CCL17
IL-20 TPO
IL-21 TRAIL/TNFSF10
IL-23 TSLP
Legend key for MILLIPLEX® map kits

† Analytes which cannot be plexed ▲ These analytes cannot be plexed with ● Serum/Plasma only
together: other analytes in this panel in serum/ ★ Tissue Culture samples only
– Active and total plasma ^ Competitive assay format
– Free and total ◗ Requires a protease inhibitor during Available for custom premix
♦ Available in Cat. No. listed sample collection
◦ Analyte requires a different sample
▼ Premix panel only ■ Requires sample extraction dilution from others in panel
Bulk indicates Space Saver Packaging ** No standard/QC; autoantibody levels  Analyte not detectable in serum
are reported as MFIs
MILLIPLEX® map IMMUNOLOGY / IMMUNE RESPONSE / METABOLISM / ENDOCRINOLOGY
Human Soluble Cytokine Multi-Species TGFβ – 3 Plex ▼ Human IgE – Singleplex

Receptor (Cat. No. TGFBMAG-64K-03) (Cat. No. HGAMMAG-303E)
(Cat. No. HSCRMAG-32K) NON-CONFIGURABLE KIT
IgE
14 (Cat. No. HSCRMAG32KPX14)
TGFβ1 TGFβ3
14 (Bulk Cat. No. HSCRMAG32PMX14BK)
TGFβ2
sCD30 sIL-6R Metabolism/

sEGFR/sHER1/ErbB1 sRAGE
sGP130 sTNF RI Immune Response Endocrinology
sIL-1RI sTNF RII Human Adipokine Panel 1
sIL-1RII sVEGFR1 / sFlt-1 Human Autoimmune (Serum/Plasma samples)
sIL-2Rα sVEGFR2 / sKDR / sFlk-1 Autoantibody**
(Cat. No. HADK1MAG-61K)
sIL-4R sVEGFR3 / sFlt-4 (Cat. No. HAIAB-10K)
Adiponectin PAI-1 (total)
β2-Glycoprotein Ribosomal P Adipsin/Factor D Resistin
Human Complement Panel 1 C1q RNP NGAL/Lipocalin-2
CENP-A (Centromere (Ribonucleoprotein)
(Cat. No. HCMP1MAG-19K)
Protein A) RNP/Smith (RNP/
Adipsin/Factor D C5a CENP-B (Centromere Sm) Human Adipokine Panel 2
C2 C9 Protein B) Scl-70 (Serum/Plasma samples)
C4b Factor I Jo-1 Sm
(Cat. No. HADK2MAG-61K)
C5 Mannose-binding
Ku SSA/Ro52 (Sjögren’s
Lectin (MBL) Mi-2 Syndrome-related
IL-1β Leptin
Myeloperoxidase antigen A/Ro52
kDa) IL-6 MCP-1/CCL2
(MPO)
SSA/Ro60 IL-8/CXCL8 NGF
Human Complement Panel 2 PCNA (Proliferating
(Sjögren’s Insulin TNFα
Cell Nuclear
(Cat. No. HCMP2MAG-19K) Antigen A) Syndrome-related
antigen A/Ro60
HUMAN
PL-12 (Alanyl-tRNA
C1q Factor B
Synthetase)
kDa) Human Adipocyte
C3 Factor H SB/La (Sjögren’s (Cell culture samples)
PM/Sci 100
C3b / iC3b Factor P/Properdin Syndrome-related
Proteinase 3 antigen B/La) (Cat. No. HADCYMAG-61K)
C4
Adiponectin MCP-1/CCL2
HGF NGF
Human MMP Panel 1 Human Sepsis Panel 1
IL-1β PAI-1 (total)
(Cat. No. HSP1MAG-63K)
(Cat. No. HMMP1MAG-55K) IL-6 Resistin
sFAS / TNFRSF6 MIF IL-8/CXCL8 TNFα
MMP-3 MMP-13
sFasL PAI-1 (total) Leptin
MMP-12
sICAM-1 sVCAM-1
Human Diabetes ▼
Human MMP Panel 2 Human Sepsis Panel 2 5 (Cat.No. HDIAB-34K-PMX5)
(Cat. No. HMMP2MAG-55K) (Bulk Cat. No. HDIAB34KPMX5BK)
(Cat. No. HSP2MAG-63K) 5
NON-CONFIGURABLE KIT
MMP-1 MMP-9
Granzyme B MIP-1α / CCL3
MMP-2 MMP-10 C-Peptide Insulin
HSP70 MIP-1β / CCL4
MMP-7
IL-1α MMP-8 GLP-1 (active) ◗ Leptin

IL-8 / CXCL8 Glucagon ◗
Human TIMP Panel 1

(Serum/Plasma samples) Human Sepsis Panel 3 Human Metabolic Hormone
(Cat. No. HTMP1MAG-54K) (Cat. No. HSP3MAG-63K) (Cat. No. HMHEMAG-34K)
TIMP-1 TIMP-2 Amylin (active) † ◗ IL-6

Lactotransferrin (LTF) Resistin
Neutrophil Elastase-2 Thrombospondin-1 Amylin (total) † ◗ Insulin
(ELA2) (TSP-1) C-Peptide Leptin
Human TIMP Panel 2 NGAL/Lipocalin-2 Ghrelin (active) ◗ MCP-1/CCL2
(Cell culture samples) GIP (total) Pancreatic
GLP-1 (active) † ◗ Polypeptide (PP)
(Cat. No. HTMP2MAG-54K)
Human Skin GLP-1 (total) † ◗ PYY (total)
TIMP-1 TIMP-3 (Skin extracts only) Glucagon ◗ TNFα
TIMP-2 TIMP-4 (Cat. No. SKINMAG-50K)
Cortisol ^ Involucrin Human Myokine

Multi-Species TGFβ – Fibronectin Keratin-1,10
(Cat. No. HMYOMAG-56K)
Singleplex Human Serum Keratin-6
Albumin (HSA) Apelin IL-15
(Cat. No. TGFBMAG-64K-01)
BDNF Irisin
(Bulk Cat. No. TGFBMAG-64K-01BK)
Erythropoietin (EPO) LIF
Human Immunoglobulin
TGFβ1 FABP3 Myostatin/GDF8
Isotyping
FGF-21 Oncostatin-M (OSM)
(Cat. No. HGAMMAG-301K) Fractalkine/CX3CL1 Osteocrin (OSTN)/
FSTL1 Musclin
IgA IgG3
IL-6 Osteonectin/SPARC
IgG1 IgG4
IgG2 IgM
10 | sigmaaldrich.com/milliplex
MILLIPLEX® map METABOLISM / ENDOCRINOLOGY / CVD / BONE / CANCER
Human Aging Panel 1 Human CVD Panel 2 Bone
(Cat. No. HAGE1MAG-20K) (Cat. No. HCVD2MAG-67K)
Human Bone
CTACK/CCL27 IL-10★ ADAMTS13 Myoglobin (Cat. No. HBNMAG-51K)
FGF-21 IL-18 D-dimer NGAL/Lipocalin-2
ACTH Osteocalcin (OC)
GDF-11 Jag1 GDF-15 sP-Selectin
DKK1 Osteopontin (OPN)
GDF-15 Leptin sICAM-1 Serum Amyloid A
FGF-23 Osteoprotegerin (OPG)
GnRH Wnt-3a Myeloperoxidase sVCAM-1
(MPO) IL-1β PTH
IL-6
IL-6 Sclerostin (SOST)
Insulin TNFα
Human Liver Protein Human CVD Panel 3 Leptin
(Acute Phase)
(Cat. No. HLPPMAG-57K)
(Cat. No. HCVD3MAG-67K) Human RANKL – Singleplex
α-Fetoprotein (AFP) FGF-19 (Cat. No. HRNKLMAG-51K-01)
α-1-Acid Glycoprotein Haptoglobin
ANGPTL3 FGF-21
(AGP) sL-Selectin
ANGPTL4 FGF-23 RANKL
α-2-Macroglobulin Platelet Factor 4
ANGPTL6 HGF
Adipsin/Factor D (PF4)/CXCL4
FABP1
CRP Serum Amyloid P
Fetuin A (SAP) Cancer
Fibrinogen 
Human IGF Binding Protein ◗ Human Immuno-Oncology
(Cat. No. HIGFBMAG-53K) Checkpoint Protein Panel 1
IGFBP1 IGFBP5
Human CVD Panel 4 (Cat. No. HCKP1-11K) NEW
17 (Cat. No. HCKP1-11K-PX17)
IGFBP2 IGFBP6 (Cat. No. HCVD4MAG-67K)
17 (Bulk Cat. No. HCKP1-11K-PXBK17)
IGFBP3 IGFBP7
sCD31/sPECAM-1 Pentraxin-3 (PTX3)
IGFBP4 BTLA HVEM
dPAPP-A Thrombomodulin
HUMAN
CD27 ICOS
sE-Selectin Tissue Factor (TF)
CD28 LAG-3
Human IGF ■ Follistatin (FST) Troponin T (TnT)
CD40 PD-1
(Cat. No. HIGFMAG-52K) CD80/B7-1 PD-L1
IGF-1 IGF-2 Human CVD Panel 5 CD86/B7-2 PD-L2

CTLA-4/CD152 TIM-3
(Cat. No. HCVD5MAG-67K)
GITR TLR-2
GITRL
Human Pituitary Panel 1 ADAM15 Leptin Receptor
Cadherin 13 (CDH13) (LEPR)/OB-R
(Cat. No. HPTP1MAG-66K) MR-ProADM Human Circulating Cancer
sCD163
ACTH FSH Chromogranin-A Notch1 Biomarker Panel 1
(CHGA/CGA) PLA2G7 (Cat. No. HCCBP1MAG-58K)
Agouti-Related GH
Protein (AgRP) HSP60 PTGDS
LH α-Fetoprotein (AFP) IL-8/CXCL8
CNTF IGF1R sST2/IL1RL1
TSH CA125 Leptin
LDLR Syndecan 4 (SYND4)
CA15-3 MIF
CA19-9 Osteopontin (OPN)
Multi-Species Hormone ■ ^
Human CVD Panel 6 CEA Prolactin
(Cat. No. MSHMAG-21K) CYFRA21-1 PSA (free)†
(Cat. No. HCVD6MAG-67K)

Cortisol Testosterone sFAS/TNFRSF6 PSA (total)†
Estradiol T3 sCD14 LRG1 sFasL SCF
Progesterone T4 DPP4 MCAM/MUC18 FGF-2/FGF-basic TGFα
Endostatin ucMGP HCGβ TNFα
pGRN HE4 TRAIL/TNFSF10
HGF VEGF-A
Cardiovascular IL-6
Human Apolipoprotein
Human CVD Panel 1
(Cat. No. HCVD1MAG-67K) (Cat. No. APOMAG-62K) Human Circulating Cancer
Biomarker Panel 2
BNP LIX/CXCL6/GCP-2 Apo AI Apo CII
Apo AII Apo CIII
(Cat. No. HCCBP2MAG-58K)
CK-MB NT proBNP
CXCL16 Oncostatin-M (OSM) Apo B Apo E Antithrombin III Extracellular Matrix
Endocan-1 (ESM-1) Placental Growth Complement Factor Protein 1 (ECM1)
FABP3 Factor (PLGF) H (CFH) Vitamin D Binding
Troponin I (TnI) Protein
FABP4
LIGHT

MILLIPLEX® map CANCER / NEUROSCIENCE / TOXICITY
Human Circulating Cancer Neuroscience Human Neuropeptide ■ ^

Biomarker Panel 3 (Cat. No. HNPMAG-35K)
Human Amyloid Beta and Tau
(Cat. No. HCCBP3MAG-58K)
(CSF samples) α-MSH Oxytocin
Cathepsin D Melanoma Inhibitory (Cat. No. HNABTMAG-68K) β-Endorphin Substance P
Ferritin Activity (MIA) Neurotensin
Fibroblast Activation Myeloperoxidase Amyloid beta 1-40 pTau (Thr181)
Protein (FAP) (MPO) Amyloid beta 1-42 Tau (total)
Galectin-3 Sex Hormone Binding Human Circadian Stress ■ ^
Globulin (SHBG)
IGFBP3
Human Neuroscience Panel 1 (Cat. No. HNCSMAG-35K)
(CSF samples)
Cortisol Melatonin
Human Circulating Cancer (Cat. No. HNS1MAG-95K)
Biomarker Panel 4
α-Synuclein PARK5/UCHL1
(Cat. No. HCCB4MAG-58K)
Glial Fibrillary Acidic PARK7/DJ1 Toxicity
ALDH1A1 Kallikrein-6 Protein (GFAP) Transglutaminase 2
Carbonic Anhydrase Mesothelin Neuron-specific (TGM2) Human Kidney Injury Panel 1
9 (CA9) Enolase (NSE) (Urine samples)
Midkine
CD44 NCAML1/L1CAM/ (Cat. No. HKI1MAG-99K)
EpCAM CD171 Human Neuroscience Panel 2
Hepsin Transglutaminase 2 Calbindin KIM-1
(Cat. No. HNS2MAG-95K)
(TGM2) Collagen IV Osteoactivin
Angiogenin (ANG)◦ Neurogranin (NRGN) FABP1 Renin
Human Cancer/Metastasis ApoE4◦ PRNP (Prion Protein) GSTα TFF-3
Biomarker Panel 1 FABP3 TREM2 IP-10/CXCL10 TIMP-1
Ferritin
(Cat. No. HCMBMAG-22K)
DKK1 Osteoprotegerin Human Kidney Injury Panel 2

Human Neurodegenerative
HUMAN
GDF-15 (OPG) (Urine samples)

Neuron-specific Periostin Disease Panel 1 (Cat. No. HKI2MAG-99K)
Enolase (NSE) TRAP5 (Cat. No. HNDG1MAG-36K)
Osteonectin/SPARC TWEAK α-1-Microglobulin EGF
YKL40/CHI3L1 α-2-Macroglobulin Complement Factor Albumin NGAL/Lipocalin-2
Apo AI C3 Clusterin Osteopontin (OPN)
Apo CIII Complement Factor Cystatin C
Human Angiogenesis/Growth H (CFH)
Apo E
Factor Panel 1
(Cat. No. HAGP1MAG-12K) Human Kidney Injury Panel 3
Human Neurodegenerative (Urine samples)
Angiopoietin-2 HB-EGF Disease Panel 2 (Cat. No. HKI3MAG-99K)
BMP-9 HGF
(Cat. No. HNDG2MAG-36K)
EGF IL-8/CXCL8 β-2-Microglobulin Uromodulin
Endoglin Leptin α-1-Antitrypsin (A1AT) MIP-4/PARC/CCL18 RBP4
Endothelin-1 Placental Growth C4 PEDF
FGF-1/FGF-acidic Factor (PLGF)
CRP Serum Amyloid P
FGF-2/FGF-basic VEGF-A (SAP) Human Kidney Injury Panel 4
Follistatin (FST) VEGF-C (Serum/Plasma samples)
G-CSF VEGF-D Human Neurodegenerative (Cat. No. HKI4MAG-99K)

Disease Panel 3
(Cat. No. HNDG3MAG-36K) EGF Osteopontin (OPN)
Human Angiogenesis Panel 2 FABP1 PTH
BDNF PAI-1 (total) IP-10/CXCL10 Renin
(Cat. No. HANG2MAG-12K)
Cathepsin D PDGF-AA KIM-1
Angiostatin/Kringle sIL-6Rα sICAM-1 PDGF-AB/BB
sAXL sNeuropilin-1 (sNRP-1) Myeloperoxidase RANTES/CCL5
sCD31/sPECAM-1 Osteopontin (OPN) (MPO) sVCAM-1 Human Kidney Injury Panel 5
sc-Kit/sStem Cell PDGF-AB/BB sNCAM (Serum/Plasma samples)
Factor Receptor Tenascin-C (TN-C)
(SCFR) (Cat. No. HKI5MAG-99K)
Thrombospondin-2 Human Neurodegenerative
sE-Selectin (TSP-2)
sEGFR/sHER1/sErbB1
Disease Panel 4 α-1-Microglobulin Osteoactivin
sTIE-2 Collagen IV TIMP-1
sHER2/sEGFR2/
(CSF samples)
suPAR NGAL/Lipocalin-2 Uromodulin
sErbB2 (Cat. No. HNDG4MAG-36K)
sVEGFR1/sFlt-1
sHER3/sEGFR3/
sErbB3 sVEGFR2/sKDR/sFlk-1
Amyloid beta 1-40 sRAGE
sHGFR/sc-Met sVEGFR3/sFlt-4 Human Kidney Injury Panel 6
Amyloid beta 1-42 S100B
(Serum/Plasma samples)
GDNF
(Cat. No. HKI6MAG-99K)
Human Neurological β-2-Microglobulin Cystatin C

Disorders Panel 3 Clusterin RBP4
(Cat. No. HND3MAG-39K)
Angiotensinogen Osteopontin (OPN) Human Liver Injury

(AGT) Soluble Superoxide (Cat. No. HLINJMAG-75K)
Contactin-1 Dismutase 1 (sSOD1)
Fetuin A ◦
Soluble Superoxide
5'NT/CD73 MDH1
Kallikrein-6 Dismutase 2 (sSOD2)
ARG1 SDH
GSTα
MILLIPLEX® map IMMUNOLOGY / METABOLISM / ENDOCRINOLOGY
PRIMATE Metabolism/ Mouse Cytokine/
Chemokine Panel 2
Endocrinology
Immunology (Cat. No. MECY2MAG-73K)
Non-Human Primate 15 (Cat. No. MECY2MAG-73KPX)
Non-Human Primate Metabolic Hormone 15 (Bulk Cat. No. MECY2MAG73KPXBK)
Cytokine/Chemokine Panel 1 (Cat. No. NHPMHMAG-45K) Erythropoietin (EPO) IL-17A/F
(Cat. No. PRCYTOMAG-40K) Exodus-2/ IL-20
Amylin (active) ◗ Insulin
23 (Cat. No. PCYTMG-40K-PX23) CCL21/6Ckine MDC/CCL22
C-Peptide Leptin
23 (Bulk Cat. No. PRCYMAG40PMX23BK) Fractalkine/CX3CL1 MCP-5/CCL12
Ghrelin (active) ◗ MCP-1/CCL2
IFNβ1 MIP-3α/CCL20
sCD40L IL-12/23 (p40) GIP (total) Pancreatic
Polypeptide (PP) IFNγ MIP-3β/CCL19
G-CSF IL-13 GLP-1 (active) ◗
PYY (total) IL-11 TARC/CCL17
GM-CSF IL-15 Glucagon ◗
IL-16 TIMP-1
IFNγ IL-17A/CTLA8 IL-6
IL-1β IL-18
IL-1Ra MCP-1/CCL2 Mouse High Sensitivity T Cell
IL-2 MIP-1α/CCL3
Non-Human Primate Pituitary (Cat. No. MHSTCMAG-70K)
IL-4 MIP-1β/CCL4 Panel 1 18 (Cat. No. MHSTCMAG-70KPMX)
IL-5 TGFα (Cat. No. NHPPT1MG-46K) 18 (Bulk Cat. No. MHSTCMAG-70KPXBK)
IL-6 TNFα
ACTH FSH GM-CSF IL-10
IL-8/CXCL8 VEGF-A
Agouti-Related GH IFNγ IL-12 (p70)
IL-10
Protein (AgRP) LH IL-1α IL-13
CNTF TSH IL-1β IL17A/CTLA8
Non-Human Primate IL-2 KC/GROα/CXCL1
Cytokine/Chemokine Panel 2 IL-4 LIX
(Cat. No. PRCYT2MAG40K) Multi-Species Hormone ■ ^ IL-5 MCP-1/CCL2
24 (Cat. No. PRCYTMAG40K-PX24) (Cat. No. MSHMAG-21K) IL-6 MIP-2/CXCL2
24 (Bulk Cat. No. PRCY2MG40PMX24BK) IL-7 TNFα
Cortisol Testosterone
25 (Cat. No. PRCYTMAG40K-PX25)
25 (Bulk Cat. No. PRCY2MG40PMX25BK)
Estradiol T3
Progesterone T4 Mouse Th17
sCD137/4-1BB/ IL-17E/IL-25
TNFRSF9 (Cat. No. MTH17MAG-47K)
IL-21
Eotaxin/CCL11 25 (Cat. No. MT17MAG47K-PX25)
IL-22
sFasL 25 (Bulk Cat. No. MT17MAG47PMX25BK)
IL-23
FGF-2/FGF-basic
Fractalkine/CX3CL1
IL-28A/IFNλ2
IL-31
MOUSE sCD40L
GM-CSF
IL-17E/IL-25
IL-17F
Granzyme A IL-33/NF-HEV
NHP / MOUSE
Granzyme B (mature) Immunology IFNγ
IL-1β
IL-21
IL-22
IL-1α IP-10/CXCL10
IL-2 MIP-3α/CCL20 Mouse Cytokine/Chemokine IL-2 IL-23
IL-4 IL-27
IL-4 Perforin Panel 1
IL-5 IL-28B/IFNλ3
IL-6 RANTES/CCL5 ▲ (Cat. No. MCYTOMAG-70K) IL-6 IL-31
IL-16 TNFβ/Lymphotoxin-α 25 (Cat. No. MCYTOMAG-70K-PMX) ♦
(LTA) IL-10 IL-33/NF-HEV
IL-17A/CTLA8 25 (Bulk Cat. No. MCYTMAG70PMX25BK) ♦
IL-12 (p70) (mature)
32 (Cat. No. MCYTMAG-70K-PX32) MIP-3α/CCL20

IL-13
Multi-Species TGFβ – 32 (Bulk Cat. No. MCYTMAG70PMX32BK)
IL-15 TNFα
Singleplex Eotaxin/CCL11 IL-13 ♦ IL-17A/CTLA8 TNFβ/Lymphotoxin-α
(LTA)
(Cat. No. TGFBMAG-64K-01) G-CSF ♦ IL-15 ♦
(Bulk Cat. No. TGFBMAG-64K-01BK) GM-CSF ♦ IL-17A/CTLA8 ♦
IFNγ ♦ IP-10/CXCL10 ♦ Mouse CD8+ T Cell
TGFβ1
IL-1α ♦ KC/GROα/CXCL1 ♦
(Cat. No. MCD8MAG-48K)
IL-1β ♦ LIF
15 (Cat. No. MCD8MAG48K-PX15)
Multi-Species TGFβ – 3 Plex ▼ IL-2 ♦ LIX
15 (Bulk Cat. No. MCD8MAG48KPX15BK)
(Cat. No. TGFBMAG-64K-03) IL-3 MCP-1/CCL2 ♦
NON-CONFIGURABLE KIT IL-4 ♦ M-CSF sCD137/4-1BB/ IL-4
IL-5 ♦ MIG/CXCL9 TNFRSF9 IL-5
TGFβ1 TGFβ3 IL-6 ♦ MIP-1α/CCL3 ♦ sFas/TNFRSF6 IL-6
TGFβ2 IL-7 ♦ MIP-1β/CCL4 ♦ sFasL IL-10
IL-9 ♦ MIP-2/CXCL2 ♦ GM-CSF IL-13
IL-10 ♦ RANTES/CCL5 ♦ Granzyme B MIP-1β/CCL4
IL-12 (p40) ♦ TNFα ♦ IFNγ RANTES/CCL5
IL-12 (p70) ♦ VEGF-A IL-2 TNFα

Mouse Soluble Cytokine Metabolism/ Multi-Species Hormone ■ ^
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® map IMMUNOLOGY / IMMUNE RESPONSE / METABOLISM / ENDOCRINOLOGY / CVD / BONE / CANCER
Receptor
Endocrinology (Cat. No. MSHMAG-21K)
(Cat. No. MSCRMAG-42K)
Mouse Adipokine
sCD30 sRAGE Estradiol T3
sGP130 sTNF RI Progesterone T4
sIL-1RI sTNF RII (Cat. No. MADKMAG-71K)
sIL-1RII sVEGFR1/sFlt-1 IL-6 PAI-1 (total)
sIL-2Rα sVEGFR2/sKDR/sFlk-1 Insulin Resistin Cardiovascular
sIL-4R sVEGFR3/sFlt-4 Leptin TNFα
sIL-6R MCP-1/CCL2 Mouse CVD Panel 1
(Cat. No. MCVD1MAG-77K)
Mouse MMP Panel 1 Mouse Adipocyte sCD31/sPECAM-1 PAI-1 (total)
(Serum/Plasma samples) (Cell culture samples) sE-Selectin sP-Selectin
(Cat. No. MMMP1MAG-79K) sICAM-1 Thrombomodulin
(Cat. No. MADCYMAG-72K)
ProMMP-9
MMP-2 MMP-8 Adiponectin PAI-1 (total)
MMP-3 IL-6 Resistin
Leptin TNFα Mouse CVD Panel 2
MCP-1/CCL2 (Cat. No. MCVD2MAG-77K)
Mouse MMP Panel 2
(Serum/Plasma samples) sCD40L Oncostatin M (OSM)
(Cat. No. MMMP2MAG-79K)

Mouse Adiponectin – CXCL16 PLGF-2
Singleplex Endocan-1 (ESM-1) Troponin I (TnI)
proMMP-9 MMP-12 (Serum/Plasma samples) Follistatin (FST) Troponin T (TnT)
LIGHT
(Cat. No. MADPNMAG-70K-01)
Mouse MMP Panel 3 Adiponectin

(Cell culture samples) Mouse Acute Phase Panel 2
(Cat. No. MMMP3MAG-79K) (Cat. No. MAP2MAG-76K)
Mouse Metabolic Hormone
MMP-2 proMMP-9 Adipsin/Factor D CRP
(Cat. No. MMHMAG-44K)
MMP-3 MMP-12 α-1-Acid Glycoprotein Haptoglobin
Amylin (active) ◗ Leptin (AGP) Serum Amyloid P
MMP-8
C-Peptide 2 MCP-1/CCL2 α-2-Macroglobulin (SAP)
Ghrelin (active) ◗ Pancreatic
Multi-Species TGFβ – GIP (total) Polypeptide (PP)
Singleplex GLP-1 (active) ◗ PYY (total)
Bone
Glucagon ◗ Resistin
TNFα
(Bulk Cat. No. TGFBMAG-64K-01BK)
IL-6 Mouse Bone
Insulin
(Cat. No. MBNMAG-41K)
TGFβ1
ACTH Leptin
Mouse Myokine DKK1 Osteoprotegerin
Multi-Species TGFβ – 3 Plex ▼ FGF-23 (OPG)
(Cat. No. MMYOMAG-74K)
(Cat. No. TGFBMAG-64K-03) IL-6 Sclerostin (SOST)
BDNF Irisin TNFα
NON-CONFIGURABLE KIT Insulin
Erythropoietin (EPO) ★ LIF
TGFβ1 TGFβ3 FGF-21 Myostatin/GDF8
TGFβ2 Fractalkine/CX3CL1 Oncostatin-M (OSM)
Follistatin-like Osteocrin (OSTN)/ Cancer
MOUSE
Protein 1 (FSTL1) Musclin

IL-6 Osteonectin/SPARC Mouse Angiogenesis / Growth
Immune Response IL-15 Factor Panel 1
Mouse Immunoglobulin (Cat. No. MAGPMAG-24K)
Isotyping
Mouse Pituitary sALK-1 IL-6
(Cat. No. MGAMMAG-300K) Amphiregulin IL-17A/CTLA8
(Cat. No. MPTMAG-49K)
IgA IgG2b Angiopoietin-2 ▲ KC/CXCL1
ACTH LH Betacellulin ▲ Leptin
IgG1 IgG3
BDNF Prolactin sCD31/sPECAM-1 ▲ MCP-1/CCL2
IgG2a IgM
FSH TSH EGF MIP-1α/CCL3
GH Endoglin Placental Growth
Endothelin-1 Factor (PLGF-2)
Mouse IgE – Singleplex Prolactin
sFasL
(Cat. No. MGAMMAG-300E) FGF-2/FGF-basic SDF-1/CXCL12
Follistatin (FST) TNFα
IgE
G-CSF VEGF-A
HGF VEGF-C
IL-1β VEGF-D
MILLIPLEX® map NEUROSCIENCE / TOXICITY / IMMUNOLOGY / METABOLISM / ENDOCRINOLOGY / CVD
Neuroscience Multi-Species TGFβ – Rat Pituitary
Singleplex (Cat. No. RPTMAG-86K)
Mouse Amyloid Beta
ACTH LH
(Cat. No. MABMAG-83K) (Bulk Cat. No. TGFBMAG-64K-01BK)
BDNF Prolactin
Amyloid beta 1-40 Amyloid beta 1-42 TGFβ1 FSH TSH
GH
Mouse Neuropeptide ■ ^ Multi-Species TGFβ – 3 Plex ▼

(Cat. No. RMNPMAG-83K) (Cat. No. TGFBMAG-64K-03)
Rat Stress Hormone ■
NON-CONFIGURABLE KIT (Cat. No. RSHMAG-69K)
α-MSH Oxytocin
β-Endorphin Substance P TGFβ1 TGFβ3 ACTH Melatonin^
Neurotensin TGFβ2 Corticosterone^

Toxicity Metabolism/
(Cat. No. MSHMAG-21K)
Mouse Kidney Injury Panel 1 Endocrinology
(Cat. No. MKI1MAG-94K) Rat Adipokine Estradiol T3
β-2-Microglobulin Renin
(Serum/Plasma samples) Progesterone T4
IP-10/CXCL10 TIMP-1 (Cat. No. RADPKMAG-80K)

KIM-1 VEGF-A
IL-1β MCP-1 / CCL2 Rat Thyroid
IL-6 PAI-1 (total) (Cat. No. RTHYMAG-30K)
Mouse Kidney Injury Panel 2 Insulin TNFα
T3^ TSH
Leptin
(Cat. No. MKI2MAG-94K) T4^
Clusterin NGAL/Lipocalin-2
Cystatin C Osteopontin (OPN)
Rat Adipocyte
(Cell culture samples)
EGF Cardiovascular
(Cat. No. RADPCMAG-82K)
Rat Cardiac Injury Panel 1
Adiponectin MCP-1/CCL2
(Cat. No. RCI1MAG-87K)
IL-1β PAI-1 (total)
RAT IL-6
Leptin
TNFα Cardiac Troponin I
(cTnI)
FABP3
Follistatin-like Protein
Cardiac Troponin T 1 (FSTL1)
Immunology (cTnT) Myosin Light Chain 3
Rat Metabolic Hormone Creatine Kinase (MYL3)
Rat Cytokine/Chemokine Muscle (CKM) TIMP-1
(Cat. No. RMHMAG-84K)
(Cat. No. RECYTMAG-65K)
Amylin (active) ◗ Insulin
27 (Cat. No. RECYMAG65K27PMX)
C-Peptide 2 Leptin Rat Vascular Injury Panel 1
27 (Bulk Cat. No. RECYMAG65PMX27BK)
Ghrelin (active) ◗ MCP-1/CCL2 (Serum/Plasma samples)
EGF IL-10 GIP (total) Pancreatic (Cat. No. RV1MAG-26K)

Eotaxin / CCL11 IL-12 (p70) GLP-1 (active) ◗ Polypeptide (PP)
Fractalkine / CX3CL1 IL-13 Glucagon ◗ PYY (total) Caveolin-1 IL-6
G-CSF IL-17A/CTLA8 IL-6 TNFα Connective Tissue MCP-1 / CCL2
Growth Factor PAI-1 (total)
GM-CSF IL-18
(CTGF)
MOUSE/RAT
GROα / KC / CINC- IP-10/CXCL10 TIMP-1
GROα / KC / CINC-
1 / CXCL1 Leptin Rat Myokine 1 / CXCL1 TNFα
IFNγ LIX VEGF
(Cat. No. RMYOMAG-88K)
IL-1α MCP-1/CCL2
IL-1β MIP-1α/CCL3 BDNF IL-6
IL-2 MIP-2/CXCL2 Erythropoietin (EPO) IL-15 Rat Vascular Injury Panel 2
IL-4 RANTES/CCL5 FGF-21 Irisin (Serum/Plasma samples)
IL-5 TNFα Fractalkine/CX3CL1 LIF (Cat. No. RV2MAG-26K)
IL-6 VEGF Follistatin-like Protein Osteocrin (OSTN)/
1 (FSTL1) Musclin Adiponectin sICAM-1
Myostatin/GDF8 Osteonectin/SPARC sE-Selectin
Rat Vascular Injury Panel 3

Legend key for MILLIPLEX® map kits (Cat. No. RV3MAG-26K)
▼ Premix panel only ■ Requires sample extraction α-1-Acid Glycoprotein Fibrinogen ▲

Bulk indicates Space Saver Packaging ^ Competitive assay format (AGP) Haptoglobin
◗ Requires a protease inhibitor during Available for custom premix α-2-Macroglobulin
sample collection (A2M)
MILLIPLEX® map BONE / NEUROSCIENCE / TOXICITY / IMMUNOLOGY / METABOLISM / ENDOCRINOLOGY
Bone Canine Cytokine/Chemokine Multi-Species TGFβ – 3 Plex ▼

(Cat. No. CCYTOMAG-90K) (Cat. No. TGFBMAG-64K-03)
Rat Bone Panel 1 13 (Cat. No. CCYTMG-90K-PX13) NON-CONFIGURABLE KIT
(Serum/Plasma samples) 13 (Bulk Cat. No. CCYTMAG90KPX13BK)
TGFβ1 TGFβ3
(Cat. No. RBN1MAG-31K)
GM-CSF IL-15 TGFβ2
ACTH Leptin IFNγ IL-18
DKK1 Osteoprotegerin (OPG) IL-2 IP-10 / CXCL10
FGF-23 PTH IL-6 KC-like
Insulin Sclerostin (SOST) IL-7 MCP-1 / CCL2
Metabolism/
IL-8 / CXCL8 TNFα Endocrinology
IL-10
Canine Gut Hormone
Neuroscience (Cat. No. CGTMAG-98K)
Equine Cytokine/Chemokine
Rat Neuropeptide ■ ^ Amylin (total) ◗ Insulin
(Cat. No. EQCYTMAG-93K)
(Cat. No. RMNPMAG-83K) 23 (Cat. No. EQCYTMG-93KPX23) Ghrelin (active) ◗ Leptin
23 (Cat. No. EQCTMG93KPX23BK) GIP (total) Pancreatic
α-MSH Oxytocin Polypeptide (PP)
GLP-1 (active) ◗
β-Endorphin Substance P Eotaxin/CCL11 IL-6 PYY (total)
Glucagon
Neurotensin FGF-2/FGF-basic IL-8/CXCL8
Fractalkine/CX3CL1 IL-10
G-CSF IL-12 (p70) Canine Pituitary Expanded
Toxicity GM-CSF IL-13 Panel
GRO IL-17A/CTLA8
(Cat. No. CANPIT-96K) NEW
Rat Kidney Toxicity Panel 1 IFNγ IL-18
IL-1α IP-10/CXCL10 ACTH LH
(Urine samples)
IL-1β MCP-1/CCL2 FSH Prolactin
(Cat. No. RKTX1MAG-37K) IL-2 RANTES/CCL5 GH TSH
Clusterin Osteopontin (OPN) IL-4 TNFα
GSTα^ TIMP-1 IL-5
IP-10 / CXCL10 VEGF-A
KIM-1 Feline Cytokine/Chemokine ▼ (Cat. No. MSHMAG-21K)
19 (Cat. No. FCYTMAG-20K-PMX) Cortisol Testosterone

19 (Bulk Cat. No. FCYTMAG20KPX19BK)
Rat Kidney Toxicity Panel 2 Estradiol T3
NON-CONFIGURABLE KIT Progesterone T4
(Urine samples)
(Cat. No. RKTX2MAG-37K) sFAS/TNFRSF6 IL-13
Flt3 Ligand IL-18
α-1-Acid Glycoprotein
(AGP)
Cystatin C GM-CSF KC/GRO Toxicity
EGF IFNγ MCP-1 / CCL2
Albumin^ NGAL / Lipocalin-2 IL-1β PDGF-BB Canine Kidney Toxicity
β-2-Microglobulin
IL-2 RANTES / CCL5 Expanded Panel 1
IL-4 SCF (Urine samples)
IL-6 SDF-1 / CXCL12 (Cat. No. CKT1MAG-97K)
Rat Liver Injury
IL-8 / CXCL8 TNFα
(Cat. No. RLI1MAG-92K) Clusterin MCP-1 / CCL2
IL-12 (p40)
Cystatin C NGAL / Lipocalin-2
5'NT/CD73 GSTα
IL-8 / CXCL8 Osteopontin (OPN)
ARG1 SDH Porcine Cytokine/Chemokine
KIM-1
GOT1 (Cat. No. PCYTMAG-23K)
RAT / OTHER
13 (Cat. No. PCYTMG-23K-13PX)

13 (Bulk Cat. No. PCYTMAG23PMX13BK) Canine Kidney Toxicity Panel
2
GM-CSF IL-6
OTHER SPECIES IFNγ

IL-1α
IL-8/CXCL8
IL-10
(Urine samples)
(Cat. No. CKT2MAG-97K)
IL-1β IL-12
Immunology IL-1Ra IL-18
Albumin RBP4
β-2-Microglobulin TFF-3
IL-2 TNFα
Bovine Cytokine/Chemokine
IL-4
Panel 1
(Cat. No. BCYT1-33K) NEW
15 (Cat. No. BCYT1-33K-PX15) Multi-Species TGFβ –
15 (Bulk Cat. No. BCYT1-33K-PXBK15) Singleplex
IFNγ IL-36RA/IL-1F5 (Cat. No. TGFBMAG-64K-01)
IL-1α IP-10/CXCL10 (Bulk Cat. No. TGFBMAG-64K-01BK)
IL-1β MCP-1/CCL2 TGFβ1
IL-4 MIP-1α/CCL3
IL-6 MIP-1β/CCL4
IL-8/CXCL8 TNFα
IL-10 VEGF-A
IL-17A
CELL SIGNALING MILLIPLEX® map ASSAYS
Cell Signaling MILLIPLEX® map Assays
Uncover how your cells communicate
We offer three kit formats in our MILLIPLEX® map Cell Signaling portfolio to meet
your diverse needs:
1. Phosphoprotein or total protein (or mixed) multiplex assays (≥ 3-plex)
2. Same protein phosphoprotein and total protein in the same assay (2-plex)
3. Singleplex MAPmate™ kits you can plex on your own
Our Cell Signaling multiplex kits give you the gift of time and sample,
for example:
10 Proteins
MILLIPLEX® Assays Western Blots
12 Samples
Number of
96-well plates or
acrylamide gels
Number of
samples per plate 12 (>40 samples can be measured in duplicate) 12
or gel
Total data points
120 (>400 data points can be measured per plate) 12
per plate or gel
Total time to
2.5 hours + overnight incubation (~18 hr) Days/weeks
result
Amount of
1-25 µg of total protein per well 10-50 µg total protein per lane
protein required
(all 10 proteins measured in same sample) (100-500 µg total protein for 10 gels)
(µg)

Akt/mTOR (Phosphoprotein) – 11 Plex Akt/mTOR (Total) – 11 Plex
(Cat. No. 48-611MAG) AB2 ▼ NON-CONFIGURABLE KIT (Cat. No. 48-612MAG) AB2 ▼ NON-CONFIGURABLE KIT
Analyte Total Phosphorylated Analyte Total Phosphorylated

Akt/PKB 3 (Ser473) H, M, R Akt/PKB 3 H, M, R
GSK3α 3 (Ser21) H, M, R GSK3α 3 H, M, R
GSK3β 3 (Ser9) H, M, R GSK3β 3 H, M, R
IGF1R 3 (Tyr1135/1136) H, M IGF1R 3 H, M, R
IR 3 (Tyr1162/1163) H IR 3 H, R
IRS1 3 (Ser636) H, R IRS1 3 H, M, R
mTOR 3 (Ser2448) H, M mTOR 3 H, M, R
CELL SIGNALING
p70S6 Kinase 3 (Thr389/412) H, M, R p70S6 Kinase 3 H, M, R

PTEN 3 (Ser380) H, M, R PTEN 3 H, M, R
RPS6 3 (Ser235/236) H, M, R RPS6 3 H, M, R
TSC2 3 (Ser939) H, M, R TSC2 3 H, M, R
●● Can be plexed with other 2 Plexes AB1: Uses Assay Buffer 1 H Human
✸ Cannot plex with other phospho Akt AB2: Uses Assay Buffer 2 M Mouse
▼ Premix panel only R Rat
Early Apoptosis – 7 Plex Human RTK (Phosphoprotein)

(Cat. No. 48-669MAG) AB2 ▼ NON-CONFIGURABLE KIT (Choose Analytes that Meet your Needs)
(Cat. No. HPRTKMAG-01K) AB1
Analyte Total Phosphorylated
Akt/PKB 3 (Ser473) H, M, R
c-Kit (pan Tyr) H
BAD 3 (Ser112) H
c-Met/HGFR (pan Tyr) H
Bcl-2 3 (Ser70) H
ErbB1/EGFR (pan Tyr) H
Active Caspase 8 3 H ErbB2/HER2 (pan Tyr) H
Active Caspase 9 3 H ErbB3/HER3 (pan Tyr) H
JNK/SAPK1 3 (Thr183/Tyr185) H, M, R ErbB4/HER4 (pan Tyr) H
p53 3 (Ser46) H FGFR1 (pan Tyr) H
Flt3 (pan Tyr) H
IGF1R (pan Tyr) H
Human Bcl-2 Family Apoptosis Panel 1 - 6 Plex IR (pan Tyr) H
(Cat. No. 48-682MAG) AB1 ▼ NON-CONFIGURABLE KIT
MSCFR (pan Tyr) H
Analyte Total Phosphorylated PDGFRα (pan Tyr) H, M, R
BAD 3 3 (Ser112) H PDGFRβ (pan Tyr) H
BAX 3 H TIE1 (pan Tyr) H
Bcl-xL/BAD 3 H TIE2 (pan Tyr) H

(interaction) VEGFR1/Flt-1 (pan Tyr) H
BIM 3 H VEGFR2/KDR/Flk-1 (pan Tyr) H
Mcl-1/BIM 3 H VEGFR3/Flt-4 (pan Tyr) H, M, R

(interaction)
Multi-Pathway (Phosphoprotein) — 9 Plex
Human Bcl-2 Family Apoptosis Panel 2 - 4 Plex
(Cat. No. 48-683MAG) AB1 ▼ NON-CONFIGURABLE KIT Analyte Total Phosphorylated
Analyte Total Phosphorylated Akt/PKB 3 (Ser473) H, M, R
Bcl-2 3 H CREB 3 (Ser133) H, M, R
Bcl-xL 3 H Erk/MAPK 1/2 3 (Thr185/Tyr187) H, M, R

Mcl-1 3 H JNK/SAPK1 3 (Thr183/Tyr185) H, M, R
NOXA/Mcl-1 3 H NFκB 3 (Ser536) H
(interaction)
p38/SAPK2A/B 3 (Thr180/Tyr182) H, M, R
Human DNA Damage/Genotoxicity – 7 Plex p70S6 Kinase 3 (Thr389/412) H, M, R
(Cat. No. 48-621MAG) AB1 ▼ NON-CONFIGURABLE KIT STAT3 3 (Ser727) H, M, R

STAT5A/B 3 (Tyr694/699) H, M, R
ATR 3 H
Multi-Pathway (Total) — 9 Plex
Chk1 3 (Ser345) H, M, R
Chk2 3 (Thr68) H
H2A.X 3 (Ser139) H, M, R Analyte Total Phosphorylated
MDM2 3 H, M, R Akt/PKB 3 H, M, R
p21 3 H CREB 3 H, M, R
p53 3 (Ser15) H Erk/MAPK 1/2 3 H, M, R
JNK/SAPK1 3 H, M, R
MAPK/SAPK (Phosphoprotein) – 10 Plex
NFκB 3 H, M, R
p38/SAPK2A/B 3 H, M, R
p70S6 Kinase 3 H, M, R
ATF2 3 (Thr71) H, M
CELL SIGNALING
c-Jun 3 (Ser73) H, M, R STAT3 3 H, M, R
Erk/MAPK 1/2 3 (Thr185/Tyr187) H, M, R STAT5A/B 3 H, M, R
HSP27 3 (Ser78) H
JNK/SAPK1 3 (Thr183/Tyr185) H, M, R
MEK1 3 (Ser222) H, M, R
MSK1 3 (Ser212) H, M
p38/SAPK2A/B 3 (Thr180/Tyr182) H, M, R
p53 3 (Ser15) H
STAT1 3 (Tyr701) H, M
NFκB — 6 Plex T-Cell Receptor (Phosphoprotein) — 7 Plex
(Cat. No. 48-630MAG) AB1 ▼ NON-CONFIGURABLE KIT (Cat. No. 48-690MAG) AB2 ▼ NON-CONFIGURABLE KIT

c-Myc 3 H CD3ε 3 (pan Tyr) H
FADD 3 (Ser194) H CREB 3 (Ser133) H, M, R
IκBα 3 (Ser32) H Erk/MAPK 1/2 3 (Thr185/Tyr187) H, M, R
IKKα/β 3 (Ser177/181) H LAT 3 (pan Tyr) H
NFκB 3 (Ser536) H, M Lck 3 (pan Tyr) H, M, R
TNFR1 3 H Syk 3 (pan Tyr) H
ZAP-70 3 (pan Tyr) H
Protein Translation — 6 Plex
TGFβ — 6 Plex
Analyte Total Phosphorylated (Cat. No. 48-614MAG) AB2 ▼ NON-CONFIGURABLE KIT
elF2a 3 (Ser51) H, M, R Analyte Total Phosphorylated

elF-4B 3 (Ser422) H, M, R Akt/PKB 3 (Ser473) H
elF-4E 3 (Ser209) H, M, R Erk/MAPK 1/2 3 (Thr185/Tyr187) H, M, R
elF-4G 3 (Ser1108) H, M, R SMAD2 3 (Ser465/467) H, M, R
4E-BP1 3 3 (Thr37/46) H, M, R SMAD3 3 (Ser423/425) H, M, R
SMAD4 3 H, M, R
Src Family Kinase Active Site TGFβRII 3 H
(Phosphoprotein) — 8 Plex
Akt Phospho/Total – 2 Plex
Analyte Total Phosphorylated (Cat. No. 48-618MAG) AB2 ▼ ✸ ●● NON-CONFIGURABLE KIT
Blk 3 (Tyr389) H
Fgr 3 (Tyr412) H, R
Akt/PKB 3 (Ser473) H, M, R
Fyn 3 (Tyr420) H, M, R
Akt/PKB 3 H, M, R
Hck 3 (Tyr411) H, M
Lck 3 (Tyr394) H, M, R
Akt1 Phospho/Total – 2 Plex
Lyn 3 (Tyr397) H, R
(Cat. No. 48-631MAG) AB2 ▼ ✸ ●● NON-CONFIGURABLE KIT
Src 3 (Tyr419) H, M, R
Yes 3 (Tyr421) H, M, R Analyte Total Phosphorylated
Akt1 (Ser473) H, M, R
STAT (Phosphoprotein) — 5 Plex Akt1 3 H, M, R

Analyte Total Phosphorylated Akt2 Phospho/Total – 2 Plex

STAT1 3 (Tyr701) H, M (Cat. No. 48-632MAG) AB2 ▼ ✸ ●● NON-CONFIGURABLE KIT
STAT2 3 (Tyr690) H Analyte Total Phosphorylated

STAT3 3 (Tyr705) H, M, R Akt2 (Ser474) H, M, R
STAT5A/B 3 (Tyr694/699) H, M, R Akt2 3 H, M, R
STAT6 3 (Tyr641) H
Akt3 Phospho/Total – 2 Plex

(Cat. No. 48-633MAG) AB2 ▼ ✸ ●● NON-CONFIGURABLE KIT
CELL SIGNALING

Akt3 (Ser472) H, M, R
Akt3 3 H, M, R
CREB Phospho/Total – 2 Plex mTOR Phospho/Total – 2 Plex

(Cat. No. 48-628MAG) AB2 ▼ ●● NON-CONFIGURABLE KIT (Cat. No. 48-625MAG) AB2 ▼ ●● NON-CONFIGURABLE KIT

CREB 3 (Ser133) H, M, R mTOR 3 (Ser2448) H, M, R
CREB 3 H, M, R mTOR 3 H, M
Erk/MAPK 1/2 Phospho/Total – 2 Plex p38 Phospho/Total – 2 Plex

(Cat. No. 48-619MAG) AB2 ▼ ●● NON-CONFIGURABLE KIT (Cat. No. 48-624MAG) AB1 or AB2 ▼ ●● NON-CONFIGURABLE KIT

Erk/MAPK 1/2 3 (Thr185/Tyr187) H, M, R p38/SAPK2A/B 3 (Thr180/Tyr182) H, M, R
Erk/MAPK 1/2 3 H, M, R p38/SAPK2A/B 3 H, M, R
IRS1 Phospho/Total – 2 Plex STAT3 Phospho/Total – 2 Plex

(Cat. No. 48-626MAG) AB2 ▼ ●● NON-CONFIGURABLE KIT (Cat. No. 48-623MAG) AB2 ▼ ●● NON-CONFIGURABLE KIT

IRS1 3 (Ser636) H, M STAT3 3 (Tyr705) H, M, R
IRS1 3 H, M, R STAT3 3 H, M, R
JNK Phospho/Total – 2 Plex

(Cat. No. 48-622MAG) AB2 ▼ ●● NON-CONFIGURABLE KIT

JNK/SAPK1 3 (Thr183/Tyr185) H, M, R
JNK/SAPK1 3 H, M, R
CELL SIGNALING
Cell Signaling MAPmate™ Assays
Plex up to 8 individual MAPmate™ assays portfolio are available to build into custom
together using our Cell Signaling Buffer and assays. For more information on our custom
Detection Kit, or include them in existing immunoassay program, contact your sales
MILLIPLEX® map Cell Signaling Panels to enhance specialist.
the panel within the guidelines provided in the
protocols. All of the analytes in the MAPmate™
Important MAPmate™ Guidelines Species

MAPmate™ Kits Homology Cat. No.
• Consult the protocol prior to use
β-Tubulin (total) H, M, R 46-713MAG
• Use the Cell Signaling Buffer & Detection Kit
GAPDH (total) H 46-667MAG
(48-602MAG), which contains all necessary
reagents except the MAPmate™ assay Akt/PKB (Ser473) H, M, R 46-677MAG
Akt/PKB (total) H, M, R 46-675MAG
• All MAPmate™ assays use Cell Signaling Assay
Buffer 2 (AB2) BAD (Ser112) H, M 46-694MAG
Caspase 3 (Active) H, M 46-604MAG
• Phospho-specific and total MAPmate™ assays
may not be plexed together ERK/MAPK 1/2 (Thr185/Tyr187) H, M, R 46-602MAG
ERK/MAPK 1/2 (total) H, M, R 46-609MAG
• GAPDH and β-Tubulin MAPmates™ can be used
for normalization with any of the MAPmate™ JNK/SAPK1 (Thr183/Tyr185) H, M 46-613MAG
or with Cell Signaling multiplex assays JNK/SAPK1 (total) H, M 46-618MAG
• Note: additional lysates are available to order mTOR (Ser2448) H, M, R 46-686MAG
upon request mTOR (total) H, M, R 46-685MAG
p38/SAPK2A/B (Thr180/Tyr182) H, M, R 46-610MAG
p38/SAPK2A/B (total) H, M, R 46-612MAG
Cleaved PARP (total) H 46-656MAG
STAT1 (Tyr701) H, M 46-655MAG
STAT1 (total) H, M 46-654MAG

CELL SIGNALING
Custom MILLIPLEX®
MILLIPLEX® map CUSTOM IMMUNOASSAYS AND SERVICES
map Assay Development

CUSTOM
Accelerate your research with

customized MILLIPLEX® map
assays designed for YOUR
samples. We offer the following
services:
• Develop new analytes
• Multiplex new analytes with existing analytes
• Rearrange existing analytes into new panels
• Design and validate assays on your samples STEP 1
Submit full protein names, species
• Kit bridging for lot-to-lot reproducibility
and sample type for feasibility
Get started today! assessment at sigmaaldrich.com/
immunoassays, “Customize Your
Assay”, then “Request Information”
button
Dream it.
Multiplex it.
Accelerate Your Research with
Download our new STEP 2
custom menu of
Customized MILLIPLEX® map Assays
Designed for YOUR Samples
Receive work plan and quotation

• Develop new analytes
• Multiplex new analytes with
existing analytes
services brochure from sales specialist
for a complete
• Rearrange existing analytes into
new panels
• Design and validate assays on
your samples
• Kit bridging for lot-to-lot
consistency
listing of services at
EMDMillipore.com/ STEP 3
customassay Place purchase order and participate
The life science business
of Merck KGaA, Darmstadt,
Germany operates as
MilliporeSigma in the
U.S. and Canada. in project kickoff call
2018-14941_Custom Assay Brochure.indd 1 9/18/18 1:54 PM
Single analyte detection customized.

When your assays require detection of single analytes
only, consider custom assay services for SMCxPRO™ and
ELISA plate readers. Leverage sub-picogram detection
capabilities of SMCxPRO™ to quantify proteins previously
undetectable in samples. For more information on
SMCxPRO™, visit sigmaaldrich.com/immunoassays.
SMCxPRO™
ELISA Plate Reader
22 | sigmaaldrich.com/immunoassays
Custom MILLIPLEX® map Analytes
MILLIPLEX® map CUSTOM IMMUNOASSAYS AND SERVICES

Human
CUSTOM
Analyte Sample
AGP (α 1-Acid Glycoprotein) Urine
Anti-Cardiolipin antibody IgA Serum/Plasma
Anti-EBV VCA IgG Serum/Plasma
Anti-Phosphatidylserine IgG, IgM Serum/Plasma
Anti-β2 Glycoprotein IgA, IgG, IgM Serum/Plasma
Apolipoprotein A-IV Serum/Plasma
BCMA (B-cell Maturation Antigen) Serum/Plasma
BRD4 Cell Lysates
Calprotectin Serum/Plasma
CBP Cell Lysates
CD13 Aminopeptidase N Serum/Plasma
Ceruloplasmin Urine
Collagen 1 Multiple
CSTB (Cystatin B) Serum/Plasma
Elafin Serum/Plasma
Galectin-1 Serum/Plasma
GRP78 Serum/Plasma
I-FABP Serum/Plasma
LCAT (Lecithin–cholesterol Acyltransferase) Serum/Plasma
LPGDS (Lipocalin like prostaglandin D synthase) Urine
p300 Cell Lysates
Procalcitonin Serum/Plasma
Semaphorin 3A Cell and Tissue Culture Supernatants
SM22/Transgelin Serum/Plasma
ST2 (Suppressor of Tumorigenicity 2) Serum/Plasma
sTM (Soluble Thrombomodulin) Serum/Plasma
Thyroxine Binding Globulin Serum/Plasma
Transferrin Urine
VE Cadherin/Cadherin 5 Serum/Plasma
Zinc-α-2 Glycoprotein Serum/Plasma
α-2 Antiplasmin Serum/Plasma
Mouse and/or Rat

Analyte Sample
sAXL Serum/Plasma
BMP-9 Serum/Plasma
BRD4 Cell Lysates
CBP Cell Lysates
HGFR/cMET Serum/Plasma
IGF-1 Serum/Plasma
p300 Cell Lysates
Periostin Serum/Plasma
Procalcitonin Serum/Plasma
Tenascin C Serum/Plasma
TRAIL Serum/Plasma
Vasopressin Plasma
Vitronectin Serum/Plasma
Multiplexed Biomarker Analysis using
LUMINEX® SYSTEMS
Luminex® Systems
Driving Assay Consistency protein analysis. As a Luminex® partner, we are
Enhance your data generation with our Luminex® a preferred distributor of Luminex® instruments,
instrument solutions. Combined with the largest accessories and software. Our ongoing dedication
portfolio of multiplex analytes available, we and industry-leading experience with multiplex
provide you the maximum power of Luminex® technology enable you to gain more information
xMAP® technology, the most trusted, widely quickly and reliably.
used platform for biomarker screening and
Luminex® xMAP® technology offers many advantages compared to other immunoassay methods:
• Multiplexing: Data generated simultaneously • Reproducibility: High-volume production
is less prone to user error, enabling the end- of xMAP® microspheres allows assay
user to construct cleaner data sets to explore standardization that solid-phased flat arrays
multiple parameters. cannot provide.
• Low sample volume: With minimal hands-on • Speed/high-throughput: Simultaneously
time, screen more than 40 analytes in a single measure the concentration of a large number
sample using a maximum of 25 µL per well. of different analytes in a single sample, in
96- or 384-well format, enabling you to
LUMINEX®
• Magnetic bead-based format: Responds

do your work faster, gaining the early and
rapidly and efficiently to a magnetic field,
comprehensive data so critical to your work.
enabling better and faster washing techniques,
including high-throughput washing options.
Specifications
Instrument FLEXMAP 3D® System Luminex® 200™ System MAGPIX® System

Cat. No. 40-014 40-012 40-072
Description The fastest, most flexible Gen 2 of the original widely The newest most cost-
platform. used Luminex® 100™ System. effective model for running
protein assays.
Software xPONENT® 4.3 xPONENT® 4.3 xPONENT® 4.3
Optics Lasers/APDs/PMTs Lasers/APDs/PMTs LED/CCD Camera
Hardware Flow Cytometry-based Flow Cytometry-based Fluorescent Imager
Bead Compatibility Magnetic and nonmagnetic Magnetic and nonmagnetic Magnetic
Multiplex Capacity 500 100 (80 for MagPlex®) 50
Read Time (Microtiter Plate) ~20 min/96-well plate ~40 min/96-well plate ~60 min/96-well plate
~75 min/384-well plate
Applications Protein/Nucleic Acid Protein/Nucleic Acid Protein/Nucleic Acid
Dynamic Range 4.5 logs 3.5 logs 3.5 logs

Dimensions including PC 110 x 62 x 63 cm 115 x 60 x 50 cm 70 x 60 x 50 cm
(W x D x H)
Weight (Analyzer) 77.1 kg (170 lbs) 49 kg (113 lbs) 17.5 kg (38.5 lbs)
CFR 21 Part 11 3 3 3
Automation 3 3 3
Security 3 3 3
LIS 3 3 3
All systems run on the Luminex® xPONENT® 4.3 data acquisition platform providing a consistent experience, from data input to
results, no matter which instrument a scientist uses for their research. This software adapts to fit the capabilities of each platform.
24 | emdmillipore.com/luminex
LUMINEX® SERVICE / CONSUMABLES / TRAINING
Instrument and Kit Service, Consumables and Training
As a preferred partner of Luminex® instruments, instrument. Our staff can help you install the
we supply full service at point of sale and instrument, train you to use your platform, and
throughout your research. Multi-level service continue to supply you with the appropriate
contracts provide cover from Luminex® reagents to run it as required.
engineers tailored to the use of your new
Service Contracts
Unlimited Unlimited
Luminex® System Remote Emergency
1 Year Warranty Plans Support Repair 1 (PM) 2 (PM) Cat. No.
FLEXMAP 3D®, Bronze • • SVCLUMBRZFM3D
FLEXMAP 3D®, Silver • • SVCLUMSLVFM3D
FLEXMAP 3D , Gold
®
• • SVCLUMGLDFM3D
FLEXMAP 3D®, Gold 360* • • SVCLUMGLD360FM3D
FLEXMAP 3D®, Platinum • • SVCLUMPLTFM3D
FLEXMAP 3D , Platinum 360*
®
• • SVCLUMPLT360FM3D
Luminex® 200™, Bronze • • SVCLUMBRZ
Luminex® 200™, Silver • • SVCLUMSLV
Luminex 200™, Gold
®
• • SVCLUMGLD
LUMINEX®
Luminex® 200™, Gold 360* • • SVCLUMGLD360
Luminex® 200™, Platinum • • SVCLUMPLT
Luminex 200™, Platinum 360*
®
• • SVCLUMPLT360
MAGPIX®, Standard • SVCLUMGLDMAGPIX
MAGPIX®, Gold • • SVCMAGPIXGOLDPM
MAGPIX , Platinum
®
• • SVCMAGPIXPLATPM
Onsite 1-business day response Onsite 2-business days response

1 (PM) = includes 1 preventive maintenance 2 (PM) = includes 2 preventive maintenances
* 360 service includes on-site support for assays developed by Luminex® only
Consumables
Description Pack Size Cat. No.
MAGPIX® Drive Fluid 4 pack, 750 mL ea MPXDF-4PK
Sheath Fluid for Luminex® 100/200™ & FLEXMAP 3D® Systems 20 L 40-50015

Sheath Fluid (20x concentrate) 1L 40-50018
MAGPIX® Calibration Kit 25 uses MPX-CAL-K25
MAGPIX Performance Verification Kit
®
25 uses MPX-PVER-K25
Luminex® 200™ Calibration Kit (xPONENT®) 25 uses LX2R-CAL-K25
Luminex® 200™ Performance Verification Kit (xPONENT®) 25 uses LX2R-PVER-K25
FLEXMAP 3D® Calibration Kit 25 uses F3D-CAL-K25
FLEXMAP 3D® Performance Verification Kit 25 uses F3D-PVER-K25
Training
Description Cat. No.
MAGPIX® System Onsite TRONSITE-MAGPIX
Installation and Training
Luminex® 200™ System Onsite TRONSITE-LX200
FLEXMAP 3D® System Onsite TRONSITE-FM3D
MILLIPLEX® Analyst 5.1 Software TRONSITE-MA
Onsite Installation and Training
BioTek® Washer Onsite Installation TRONSITE-BIOTEK
and Training
MILLIPLEX® map Kits Training TRONSITE-MPX
emdmillipore.com/luminex | 25
BioTek® Washing & Dispensing Solutions for
WASHING & DISPENSING FOR MILLIPLEX® map ASSAYS
MILLIPLEX® map Assays

In partnership with BioTek®, we offer the latest advancements in fully automated magnetic
separation for 96- and 384-well plate washing systems. The MultiFlo™ FX allows users to dispense
kit reagents, ensuring excellent assay reproducibility.
BioTek® washer advantages:

• Fast and hands-free full plate washing
• The BioTek® 405™ TS models in 96- and 384-
well options include an intuitive touch-screen
user interface and the Ultrasonic Advantage™
for easy washing of the toughest sample types
BioTek® 405™ Select TS (touch screen) Washer

BioTek® 405™ LS Magnetic 96-well Washer 40-094
BioTek 405™ LS Magnetic/Vacuum Filtration 96-well Washer
®
40-095
BioTek® 405™ TS Magnetic 96-well Washer Complete with Touch Screen and Ultrasonic Cleaning 40-096
BioTek® 405™ TS Magnetic/Vacuum Filtration 96-well Washer Complete with Touch Screen and Ultrasonic Cleaning 40-097
BioTek® MultiFlo™ FX Automated

Reagent Dispenser
The perfect instrument for MILLIPLEX® map 384-well
Assays
• Fully automated process, just load your standards, QCs
and samples and walk away
• Optimized for both 384- and 96-well plates
• The performance of a larger robot for a fraction of the

cost
• Self-contained, programmable reagent dispenser enables
BIOTEK®
precise liquid delivery

BioTek® MultiFlo™ FX Automated Reagent Dispenser 40-099
Handheld Magnetic Separator Block for

96-well Flat Bottom or Conical Well Plates
A low-cost alternative to automated plate washers without
loss of assay performance. Simply decant or “flick” the liquid
contents and blot the remainder on a paper towel. Magnetic
beads are securely held in the wells by the block magnets.

Handheld Magnetic Separator Block for 96-well Flat Bottom 40-285
or Conical Well Plates
26 | emdmillipore.com/biotek
SMC™ (SINGLE MOLECULE COUNTING) TECHNOLOGY
SMC™ Technology
Single Molecule Counting (SMC™) Technology: Reduced
background + increased signal
SMC™
SMC™ technology provides maximum to-noise ratios over traditional immunoassay
immunoassay performance while following a technologies. The SMC™ technology thus
workflow similar to traditional ELISA technology, provides enhanced quantification at both low
as shown below. By combining a unique assay and high levels of expression on one complete
elution step and robust digital counting, system.
SMC™ technology achieves improved signal-
STANDARD PROPRIETARY
1 Analyte of Interest in 2 Capture on 3 Detect 4 Elute 5 Quantify

Solution Plate or Bead Complex is chemically Sample is detected by laser;
broken apart detection tags counted
Steps 2-3 Step 4 Step 5

Antibodies translate each Fluorescent dye-labeled On the SMCxPRO™
biomarker into a signal detection antibodies instrument, signal is
dissociate from the collected from free-floating
immunocomplex fluorochromes using a
rotating laser, camera and
counting individual molecules
by an Avalanche Photodiode
Digital counting improves sensitivity and dynamic range

The SMCxPRO™ and Erenna® instruments capture the sum of all digital events counted above

the background threshold. The digital event algorithm of the SMCxPRO™ instrument computes
digital events across a spectrum of time series for a single standard. On the Erenna®, at lower
concentrations, photons are digitally counted as single molecules. At higher concentrations, molecules
are counted in analog and a proprietary algorithm computes the total sum of all photons recorded.
The digital counting of molecules thus improves the assay sensitivity and extending the assay dynamic
range beyond what can be achieved with traditional methods.
Validation Criteria for Verified Immunoassays

A comprehensive set of criteria evaluating ultimate quantitative performance are
used to qualify verified immunoassays
• Lower limit of quantitation: Lowest point on • Spike recovery: Minimum of 10 samples
standard curve with CV <20% and accuracy spiked with acceptable recovery between
within 20% of expected values 80–120%
• Inter- and intra-assay precision: Samples • Dilutional linearity and parallelism (where
run on multiple plates over multiple days. feasible): Assays within 20% of expected value
Spiked and un-spiked samples within 20% • Endogenous range: Minimum of 10 samples
across experiments from individual healthy donors assessed for
biomarker levels for each verified assay and
lot to lot
emdmillipore.com/smctech | 27
SMC™ Immunoassay Kits
SMC™ (SINGLE MOLECULE COUNTING) ASSAYS
LLOQ Median Sample MTO or

Analyte (pg/mL)1 Endogenous (pg/mL)2 Species3 Type4 STOCK Cat. No.
SMC™
Akt1 (Ser473) 0.98 NA H, M, R L MTO 03-0100-01

Akt1 (total) 7.80 NA H, M, R L MTO 03-0099-01
Amyloid beta 1-40 6,7
5.86 Normal CSF: 1366; P: 152 H, M, R C, P STOCK 03-0145-00
Amyloid beta 1-426,7 0.98 Normal CSF: 192; P: 15 H, M, R C, P STOCK 03-0146-00
cTnI6,7 0.35 P: 3.04; S: 3.02 H, Cy, R, C, GP P, S STOCK 03-0154-00
G-CSF 0.08 17 H P MTO 03-0047-00
GM-CSF 0.02 0.2 H P MTO 03-0067-00
GLP-1 (active) 0.40 3.46 H, M, R, C P MTO 03-0024-03
GLP-1 (total) 0.39 17.8 H, M, R, C P MTO 03-0025-06
Glucagon6,7 0.78 44 H, M, R P STOCK 03-0153-00
IFN-γ 0.20 0.79 H P STOCK 03-0049-00
IL-1α6,7 NEW 0.12 P: 0.09; S: 0.10 H P,S STOCK 03-0167-00
IL-1β6,7 0.20 P: 0.11; S: 0.12 H P,S STOCK 03-0160-00
IL-2 0.05 0.21 H P, S MTO 03-0051-00
IL-46,7 0.04 P: 0.18; S: 0.18 H P,S STOCK 03-0161-00
IL-56,7 NEW 0.04 P: 0.37; S: 0.30 H P,S STOCK 03-0168-00
IL-66,7
0.08 P: 0.70; S: 0.69 H P, S STOCK 03-0155-00
IL-7 0.39 4.91 H P, S MTO 03-0094-00
IL-8 / CXCL8 0.24 3.6 H P, S MTO 03-0055-00
IL-12 0.05 0.13 H P, S STOCK 03-0057-00
IL-13 6,7
0.04 P: 0.37; S: 0.33 H P,S STOCK 03-0156-00
IL-15 0.10 3.38 H P, S MTO 03-0058-00
IL-17A6,7 0.07 P: 0.31; S: 0.30 H P, S STOCK 03-0159-00
IL-17F6,7 0.20 P: 1.10; S: 1.18 H P, S STOCK 03-0164-00
IL-17A/F Heterodimer6,7 NEW 1.17 P: 1.36; S: 3.20 H P,S STOCK 03-0177-00
IL-21 0.20 0.53 H S MTO 03-0014-07
IL-236,7 0.10 P: 0.65; S: 0.46 H P,S STOCK 03-0157-00

KIM-1 5
3.91 P: 65; S: 75; U: 147 H P, S, U MTO 03-0118-00
TNFα6,7 0.20 P: 0.85; S: 0.78 H P,S STOCK 03-0163-00
TNFα 0.40 38.6 M S MTO 03-0108-00
VEGF-A NEW 0.20 P: 48; S: 50 H P,S STOCK 03-0180-00
1
LLOQ: Lowest point on standard curve with CV < 20% and accuracy within 20% of expected values.
2
Median Endogenous: Minimum of 10 samples from individual donors assessed for ability to quantify baseline biomarker values.
3
Optimized for first species type listed. Other listed species have been tested, but not optimized for peak performance.
Key: H=Human; M=Mouse; R=Rat; GP=Guinea Pig; Cy=Cynomolgus Monkey; C=Canine
4
Optimized for use in sample type(s) listed. Key: P=Plasma; S=Serum; L=Lysate; U=Urine; C=Cerebrospinal Fluid (CSF)
5
Plate-based assay.
6
Assay characteristic data is representative for both Erenna® and SMCxPRO™.
7
New streamlined format kit. For list of changes refer to the new kits section.
For a complete list of kits qualified on both the SMCxPRO™ and Erenna® instruments,
visit sigmaaldrich.com/smcxpro
28 | emdmillipore.com/smctech
Mouse Plate-based Discovery
Immunoassay Kits
SMC™
Discovery immunoassays are provided as complete kits to run on your
Erenna® platform
• Reproducible LLOQ • Minimal sample volume
• Simple workflow
LLOQ Median Endogenous

Analyte (pg/mL) (pg/mL) Species Sample Type Cat. No.
Mouse IL-4 0.49 NA M S 03-0136-00
Mouse IL-5 0.24 6.1 M S 03-0132-00
Mouse IL-10 6.2 8.3 M S 03-0134-00
Mouse IL-13 3.91 12.0 M S 03-0133-00
Mouse IL-17A 0.98 9.2 M S 03-0123-00
Mouse IL-17F 0.49 2.2 M S 03-0125-01
Mouse IL-21 4.9 44.0 M S 03-0126-00
Mouse IL-22 100 195 M S 03-0127-00
Mouse TNFα 0.49 28.4 M S 03-0137-00
Aurora High-Performing Microplates for Use on the

SMCxPRO™ System
Partnering with Aurora Microplates, Inc., we are the

exclusive distributor of the High-Performance
384-Well Microplate- specifically packaged for use on
the SMCxPRO™. Enhanced detection and stability of
your biological materials are critical for generating

consistent, high quality data. Containing a
flat-bottom design with a high transmittance
window, Aurora plates are optimized for imaging.
Available in two convenient package sizes.

384-well Aurora plate (Case of 32 plates) ABB2-00160A
One 384-well Aurora plate w/ 3 plate seals 02-1008-00
New SMC™ Application Highlight for
Immunogenicity
Detecting negative immunogenic responses Using SMC™ technology combined with our
early in the drug discovery process is critical unique immunogenicity assay development
to ensure potential immune responses are kit, researchers can develop a homogenous
identified promptly. Informed decisions can then species independent bridging assay that is
be made as companies seek to develop effective simple, easy to design and validate. The SMC™
medicines, with improved clinical efficacy and Immunogenicity bead-based assay development
patient safety, to critical diseases. Assessing kit includes all you need to develop and validate
the immunogenicity of a compound occurs via an ADA assay. The comprehensive assay
the detection of anti-drug antibodies (ADA), development protocol is designed to guide and
specifically IgG, IgM and IgE. FDA guidelines assist you with the process. Our experienced
SMC™
continue to stress the importance of high application scientists can also help design,
sensitivity as this leads to earlier detection of a validate and also train end-users to fully utilize
primary immune response which is essential for the platform.
patient safety.
Bridging Assay Format SMC™ Technology

Alexa fluor Eluate
conjugated
drug
Capture Wash Detection
Anti-Drug
Antibody
(in-sample)
Biotinylated
Drug
Offline Sample Complex Capture Elution Single Molecule

Incubation Counting
• Complex is captured • Complex is dissociated,
• ADA in sample is onto blocked beads beads are magnetically • Rotating laser scans
incubated for 2hr or separated and eluate sample
overnight • Wash to remove transferred to read
unbound antibodies • Alexa-conjugated drug
plate.
is excited and photons
generated is counted
by an APD

SMC™ Immunogenicity Bead Based Assay Development Kit 03-0175-00
384-well Aurora plate (Case of 32 plates) ABB2-00160A
One 384-well Aurora plate w/ 3 plate seals 02-1008-00
Kit includes reagents for approximately 30 plates (96-well). Includes all

labeling reagents and buffers specific for optimizing the assay.
SMC™ (SINGLE MOLECULE COUNTING) CUSTOM ASSAYS AND SERVICES
Improve your Sensitivity with
SMC™ Homebrew Assays
Take advantage of our ultrasensitive Single Molecule
Counting (SMC™) technology while developing your own
application-specific immunoassays in either bead or plate
format. Our assay development and optimization kits provide
everything you need to build your own high performing SMC™
immunoassay for use with the SMCxPRO™ system. Use our
Better Sensitivity..... Femtogram/mL
platform to customize the assay required to accelerate your
research. We offer hands-on assay development training for
Better
Improve Sensitivity.....
your homebrew Femtogram/mL
SMC™ at your facility or one of our global training centers.
assays with SMC™ assay development kits
CUSTOM
Improve your homebrew assays with SMC™ assay development kits
Custom high-sensitivity assay development is as easy as …

Custom high-sensitivity assay development is as easy as …
EP Signal vs. Expected Concentration
108
EP Signal vs. Expected Concentration
108
107
107
EP Signal
EP Signal
106
106
105 105
104
104 10-3 10-3 10-3 10-0 10-1 10-2 103
10-3 10-3 10-3 Expected10-0
10-1
Concentration
10-2 103
Expected Concentration
Q1: Standard Group 2
Q1: Standard Group 2
Label Test Discover

Label Test Discover
Conjugate your antibodies Verify your assay Analyze and discover
Conjugate your antibodies Verify your assay Analyze and discover
Our SMC™ assay development kits provide everything you need to develop your own femtomolar-range
immunoassay for use with the SMCxPRO® System.
Our SMC™ assay development kits provide everything you need to develop your own femtomolar-range
immunoassay for use with the SMCxPRO® System.

Contact your local MilliporeSigma
Personalized Representative and ask how our kits
Assays can save you
Contact time
your and MilliporeSigma
local money.
Personalized SMC™ Assay Development Training – 3 days 96-2024-00
Representative
Name and ask how our kits
SMC™ Assay Development Training – extra day 96-2025-00
Assays
Reliable Better can save you time and money.
Phone number
SMC™ Immunoassay Development Kit 03-0078-00
Field Support Sensitivity
SMC™ Detection Labeling Kit Name
Email address 03-0076-02
Reliable Better SMC™ Capture Labeling Kit Phone number 03-0077-02
Field Support Simplistic

Sensitivity SMC™ Assay Buffer Optimization Kit 03-0122-00
Email address
Design
Simplistic
Design
EMDMillipore.com/smctech
The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada.
© 2019 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. MilliporeSigma, the vibrant M, Millipore, and SMC are
EMDMillipore.com/smctech
trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners. Detailed
information on trademarks is available via publicly accessible resources.
Lit. No. MS_FL3801EN Ver. 1.0 2019 - 19901 05/2019
The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada.
© 2019 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. MilliporeSigma, the vibrant M, Millipore, and SMC are
trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners. Detailed
2019 - on
information 19901_RS_IPS
trademarks SMC HomeBrew-
is available Flyer Version
via publicly 2_update_MSIG.indd
accessible resources. 1 21/05/19 9:53 PM
Lit. No. MS_FL3801EN Ver. 1.0 2019 - 19901 05/2019
Expert Custom Assays & Sample Testing
SMC™ (SINGLE MOLECULE COUNTING) CUSTOM ASSAYS AND SERVICES
(CAST) for SMC™ Technology

We understand you don’t always have the time or resources to develop your own assay for
your protein of interest or to test your critical samples. Our CAST team has over 130 years of
immunoassay development experience with core expertise in SMC™ technology. Let us help you
accelerate your program from discovery research into clinical trials with a custom designed program
for you.
Our services include:

Collaborative Biomarker Discovery Reagent Selection
& Development • Reagent sourcing
• Preclinical to early clinical sample testing • Screening and selection
• Immunoassay development • Antibody derivatization
• Biomarker verification to sponsor requirements • QC generation
Biotherapeutic Development Sample Testing

• Pharmacokinetics • Prototype sample testing
• Pharmacodynamics • Proprietary kit sample testing
CUSTOM
• Immunogenicity • SMC™ immunoassay kit testing
Utilize our SMCxPlore Services, customized streamlined options to “explore” SMC™ technology with
your analyte of interest or critical samples.
SMCxPlore Assay Development SMCxPlore Assay Conversion to

Feasibility: SMC™:
Development of a prototype assay using your With access to over 600 proven antibody pairs,
antibody pairs and standard. Ideal for bringing let us develop a SMC™ assay that isn’t currently
your assay in house. available.
SMCxPlore Proof of Concept Sample

Testing:
Sample testing of up to 30 of your critical
samples using one of our highly validated SMC™

immunoassays.
SMC™ (SINGLE MOLECULE COUNTING)
SMCxPRO™ Immunoassay System Specifications
SMCxPRO™ & Ancillary Product Services Cat. No.

SMCxPRO™ Complete System 95-0100-00
Standard SMCxPRO™ Installation 96-2011-00
SMCxPRO™ System Training – 1 day 96-2022-00
SMC™ Advanced Training – 3 days 96-2023-00
SMCxPRO™ IQ/OQ Installation 96-2012-00
SMCxPRO™ PQ Service 96-2013-00
BioTek® 405 Touch Microplate Washer with MAG plate 95-0004-05
Tecan Hydroflex Microplate 8-Channel Washer 95-0014-00
Jitterbug Plate Shaker 70-0009-00
Software is 21CFR part II compliant and LIMS compatible
Network/PC Requirement Three Levels of Onsite Service

• Microsoft Windows 7/10 Operating Systems
® Support for SMCxPRO™ Platform
• A static IP address and an FTP server • Total Service: Unlimited visits including labor,
• xPRO Operating and Analysis Software travel, expenses and all parts for any service
included (32- or 64-bit compliant) for one year
• Advanced Service: Labor, travel and expenses
Read Plate Format for one preventative maintenance and one
floating visit for one year
SMCxPRO™
• 384-well plate
• Essential Service: Labor for one preventative
maintenance visit for one year
Assay Format
• Bead-based assays Minimum Instrument Performance
Specifications
• Plate-based assays

Metric Specification
Instrument Specifications Slope >1.2 response/fM1

Background <3 response/fM1
• Instrument dimensions: 406.4 mm H x 355.6
mm W x 444.5 mm D (16"H x 14"W x 17.5"D) Limit of Detection (LoD) <1 fM
• Weight: 22.7 kg (50 lbs) Precision <10% CV2
• US: 115 VAC, 50-60 Hz Dynamic Range >4 logs

(Operating range 90-125 V) 1
etermined from calibrator set: 0-300 fM of 150 kD antibody labeled
D
• International: 230 VAC, 50-60 Hz with fluorophore
2
Response measurements from 30fM calibrator, n=20
(Operating range 180-250 V)

• Set up of unit on bench and complete
calibration of unit
• Run ASSIST program to verify instrument
performance
• Optional IQ/OQ and PQ Services available
emdmillipore.com/smcxpro | 33
Ancillary Equipment to Optimize your SMC™ Assays
SMC™ (SINGLE MOLECULE COUNTING) ANCILLARY EQUIPMENT
BioTek® 405TS 384 Well Plate Washer
In partnership with BioTek®, we offer the latest

advancements in 96-well plate washing: a fully
automated system designed to quickly wash an
entire plate through biomagnetic separation, washing
and vacuum filtration. The system comes specifically
optimized for use with SMC™ assays with the
inclusion of a specially designed magnetic plate and
customized pre-programmed plate washing proto-
cols. The 405™ TS model offers an easy-to-use and
glove touchable screen.

BioTek® 405™ TS Magnetic 96-well 95-0004-05
Washer Complete with Touch Screen and
Ultrasonic Cleaning
Boekel Scientific Jitterbug, 130000, Heated Microplate Shaker (115V)
The microplate shaker is temperature controlled and

ideal for immunoassays like SMC™. Includes an inner
lid to eliminate light and reduce the risk of contami-
nation.

Boekel Jitterbug Heated Microplate Shaker 70-0009-00
(115V)
Boekel Jitterbug Heated Microplate Shaker 70-0009-00A
(230V)
SMC™
Tecan® HydroFlex™ 8 Channel Plate Washer
The HydroFlex™ microplate washer is a flexible

washer with automated microplate strip washing,
magnetic bead washing and vacuum filtration in
96-well plate formats. Along with microplate shaking
and automated buffer switching included, the system
comes specifically optimized for use with SMC™
assays with the inclusion of a specially designed
magnetic plate and customized pre-programmed
plate washing protocols.

Tecan HydroFlex™ 8 Channel Plate
®
95-0014-00
Washer
ELISAs
Millipore Brand ELISAs
Metabolic/Endocrine ELISAs
Our menu of metabolic and endocrine ELISAs allows end-users a consistent and reliable method of
investigating metabolic disease states or defining outcomes within toxicological studies during drug
development.
Standard Sample Bulk Packaging

Description Species Curve Range Sensitivity Volume Cat. No. Cat. No.*
Adiponectin Human 1.56–200 ng/mL 0.2 ng/mL 10 µL EZHADP-61K EZHADP-61BK
Adiponectin (High Human 1.5–200 ng/mL 0.5 ng/mL 10 μL EZHMWAN-65K
Molecular Weight)
Adiponectin Mouse 1–50 ng/mL 0.2 ng/mL 10 µL EZMADP-60K EZMADP-60BK
Adiponectin Rat 3.125–200 ng/mL 0.4 ng/mL 10 µL EZRADP-62K
Amylin (active) Human 1–100 pM 0.7 pM 50 µL EZHA-52K
C-Peptide Human 0.2–20 ng/mL 0.05 ng/mL 10 µL EZHCP-20K EZHCP-20BK
C-Peptide Canine 0.2–10 ng/mL 0.24 ng/mL 25 µL EZCCP-47K
C-Peptide 2 Mouse, Rat 25–1600 pM 15.0 pM 20 µL EZRMCP2-21K
C-Reactive Protein (CRP) Human 0.12-10 ng/mL 0.2 ng/mL 2 μL CYT298
C-Reactive Protein (CRP) Rat 4.9-10 ng/mL 2.5 ng/mL 2 μL CYT294
FGF-21 Human 31.25–2000 pg/mL 10.0 pg/mL 50 µL EZHFGF21-19K
FGF-21 Mouse, Rat 49.4–12,000 pg/mL 10.0 pg/mL 10 µL EZRMFGF21-26K
FGF-23 Human 9.9–2400 pg/mL 3.5 pg/mL 50 µL EZHFGF23-32K EZHFGF23-32BK
Ghrelin (active) Human 25–2000 pg/mL 15.0 pg/mL 20 µL EZGRA-88K EZGRA-88BK
Ghrelin (active) Mouse, Rat 25–2000 pg/mL 8.0 pg/mL 20 µL EZRGRA-90K
Ghrelin (total) Human 100–5000 pg/mL 50.0 pg/mL 20 µL EZGRT-89K EZGRT-89BK
Ghrelin (total) Mouse, Rat 0.1–10 ng/mL 0.04 ng/mL 20 µL EZRGRT-91K
GIP (total) Human 8.2–2000 pg/mL 4.2 pg/mL 20 µL EZHGIP-54K EZHGIP-54BK
GIP (total) Mouse, Rat 8.2–2000 pg/mL 4.2 pg/mL 10 µL EZRMGIP-55K EZRMGIP-55BK
GLP-1 (active) Multi-Species 2–100 pM 1.0 pM 100 µL EGLP-35K EGLP-35BK
ELISAs
GLP-1 High Sensitivity Multi-Species See data sheet 0.14 pM 50 μL EZGLPHS-35K EZGLPHS-35BK
(active) ∆••
GLP-1 (total) Multi-Species 4.1–1000 pM 1.0 pM 20-50 μL EZGLP1T-36K EZGLP1T-36BK
GLP-2 Multi-Species 1–64 ng/mL 0.3 ng/mL 50 µL EZGLP2-37K
Glucagon ∆ Human, 0.02–2 ng/mL 0.003 ng/mL 150-300 μL EZGLU-30K EZGLU-30BK
Mouse, Rat

Growth Hormone (GH) Mouse, Rat 0.7–50 ng/mL 0.07 ng/mL 10 µL EZRMGH-45K EZRMGH-45BK
Insulin Human 2–200 µU/mL 1.0 μU/mL 20 μL EZHI-14K EZHI-14BK
Insulin Mouse, Rat 0.2–10 ng/mL 0.1 ng/mL 10 μL EZRMI-13K EZRMI-13BK
Insulin (Animal Human 2–200 µU/mL 0.85 μU/mL 20 μL EZHIASF-14K
serum free)
Leptin Canine 0.78–50 ng/mL 0.21 ng/mL 20 µL EZCL-31K
Leptin Mouse 0.2–30 ng/mL 0.05 ng/mL 10 µL EZML-82K EZML-82BK
Leptin Rat 0.2–30 ng/mL 0.08 ng/mL 10 µL EZRL-83K EZRL-83BK
Leptin “Dual Range” Human 0.5–100 ng/mL 0.2 ng/mL 25 µL EZHL-80SK EZHL-80BK
Procollagen Type IIA Human lot dependent 30.0 ng/mL 5 µL EZPIIANP-53K
N-Propeptide (PIIANP)
Proinsulin (total) Human 2–200 pM 0.5 pM 20 µL EZHPI-15K EZHPI-15BK
PYY (total) Human 10–2000 pg/mL 6.5 pg/mL 20 µL EZHPYYT66K
Resistin Human 0.16–5 ng/mL 0.02 ng/mL 20 μL EZHR-95K EZHR-95BK
SAA-3 Mouse 0.078-5 μg/mL 0.078 μg/mL 10 μL EZMSAA3-12K
* Bulk packaging available on select kits — more environmentally friendly and saves space (10 kit equivalent)
•• Preferred assay for measuring GLP-1 (active)
∆ Chemiluminescent assay
sigmaaldrich.com/elisa | 35
Millipore Brand ELISAs & RIAs
ELISAs AND RIAs
Neuroscience ELISAs
Get a complete picture of the complexities associated with normal and disease states of the nervous
system with reliable quantification of biomarkers using our neuropeptide and neurodegenerative ELISAs.
Standard Sample
Description Species Curve Range Sensitivity Volume Cat. No.
Amyloid beta 1-40 Human 16–500 pg/mL 4 pg/mL 50 µL EZHS40
Amyloid beta 1-42 Human 16–500 pg/mL 5 pg/mL 50 µL EZHS42
Amyloid beta, Set Human Contains 1 each of EZHS40 and EZHS42 EZHS-SET
Amyloid beta (Brain) 1-40 Human 16–500 pg/mL 4 pg/mL 50 µL EZBRAIN40
Amyloid beta (Brain) 1-42 Human 16–500 pg/mL 5 pg/mL 50 µL EZBRAIN42
Amyloid beta (Brain), Set Human Contains 1 each of EZBRAIN40 and EZBRAIN42 EZBRAIN-SET
BDNF (Brain-Derived Human, Rat 7.8–500 pg/mL 7.8 pg/mL 50 µL CYT306

Neurotrophic Factor)
GFAP (Glial Fibrillary Human, Mouse, Rat 1.5-100 ng/mL 1.5 ng/mL 100 μL NS830
Acidic Protein)
NGF (Nerve Growth Factor) Mouse, Rat 10–1000 pg/mL 10-15 pg/mL 50 µL CYT304
NPY (Neuropeptide Y) Human 5-1000 pg/mL 2 pg/mL 50 µL EZHNPY-25K
NPY (Neuropeptide Y) Mouse, Rat 0.01-2 ng/mL 0.004 ng/mL 20 µL EZRMNPY-27K
PEDF (Pigment Epithelium- Human 0.9–62.5 ng/mL 0.9 ng/mL 50 µL CYT420

Derived Factor)
Phosphorylated Multi-Species 0.0293–15 ng/mL 0.0585 ng/mL 1-10 µL NS170

Neurofilament,
(pNF-H) Sandwich
S100B Human 2.7-2000 pg/mL 1.3 pg/mL 50 µL EZHS100B-33K

ELISA / RIA
Radioimmunoassays (RIAs)
RIAs have long been considered a valuable, economical and accurate way to measure protein
concentration. As many researchers move away from using radioactive material, please consider
using our other high quality immunoassays for your research needs.
Standard Sample
Description Species Curve Range Sensitivity Volume Cat. No.

Adiponectin Human lot dependent 1 ng/mL 5 µL HADP-61HK
C-Peptide Canine 0.156–20 ng/mL 0.15 ng/mL 50 µL CCP-24HK
C-Peptide Human 0.1–5 ng/mL 0.1 ng/mL 50 µL HCP-20K
Ghrelin (active) Human lot dependent 7.8 pg/mL 50 µL GHRA-88HK
Ghrelin (total) Human lot dependent 93 pg/mL 50 µL GHRT-89HK
GLP-1 (active) Multi-Species 10–500 pM 3 pM 300 µL GLP1A-35HK
GLP-1 (total) Multi-Species 10–1000 pM 3 pM 300 µL GLP1T-36HK
Glucagon Multi-Species 20–400 pg/mL 20 pg/mL 50 µL GL-32K
Insulin Porcine 2–200 µU/mL 2 µU/mL 50 µL PI-12K
Insulin Rat 0.1–10 ng/mL 0.1 ng/mL 50 µL RI-13K
Insulin LisPro Multi-Species 2.5–250 µU/mL 2.5 µU/mL 100 µL LPI-16K
Insulin (sensitive) Rat 0.02–1 ng/mL 0.02 ng/mL 50 µL SRI-13K
Insulin Specific Human 2–200 μU/mL 2 μU/mL 50 μL HI-14K
Leptin Human 0.5–100 ng/mL 0.5 ng/mL 50–100 µL HL-81K
Leptin Multi-Species 1–50 ng/mL 1 ng/mL 50 µL XL-85K
Proinsulin Human 2–100 pM 2 pM 100–200 µL HPI-15K
PYY (3-36) Human lot dependent 20 pg/mL <100 µL PYY-67HK
PYY (total) Human lot dependent 10 pg/mL <100 µL PYYT-66HK
36 | sigmaaldrich.com/elisa
SEARCH OUR ELISAs ONLINE
Sigma-Aldrich Brand ELISAs
We offer a portfolio of > 1,800 ELISAs and EIAs, covering a diverse range of species and sample
types. A variety of research areas are addressed, from inflammation and cardiovascular disease to
metabolic and veterinary research. With such a large number of ELISAs, it is impossible to list them
all on a single page, so search your target at SigmaAldrich.com. To view protocols and performance
parameters, type “sample” into the Certificate of Analysis box and open the PDF.
ELISA Designations
• Sandwich ELISA ––Upcharge may apply
––Standard off-the-shelf assay • EIA
––Some may be designated “lysate” to ––Competitive ELISA
accommodate the change from serum, • Cell-Based Phosphorylation ELISA
plasma or supernatant
––For use on cell lysates to detect
• Custom Sandwich ELISA phosphorylation events
––Requires custom manufacture and validation
Cytokines Metabolic Cardiovascular Angiogenesis Signaling
✔ ✔ ✔ ✔ ✔
✔ ✔ ✔ ✔ ✔
✔ ✔ ✔ ✔
ELISAs
✔
✔ ✔

✔
✔ ✔
sigmaaldrich.com/elisa | 37
ELISA Reading and Washing Equipment in
ELISA READER / WASHER
Partnership with BioTek®

Supporting our collection of over 1800 ELISA products, we are pleased to introduce the 800 TS
Absorbance Reader and 50 TS Plate Washer which together provide a cost effective addition to your
ELISA work flow.
BioTek® 800 TS Absorbance Reader

For EMD Millipore and Sigma-Aldrich ELISAs
• Precise, accurate and versatile, used for many

applications, including ELISA, protein and
other end-point assays, plus kinetics and
cell-based assays
• Software options: Basic, Gen 5 and Gen 5
Secure
• Flexible filter options; 450nm, 590nm, 630nm
and 750nm as standard
• Optional absorbance test NIST traceable plate
• Color touchscreen for quick programming and
simple operation
• USB flash drive for convenient data export
• Durable and high quality design

BioTek® 800 TS Absorbance Reader, 40-006
Gen 5 Software
BIOTEK®

Gen 5 Secure
Basic
BioTek® 50 TS Plate Washer

BioTek® 50 TS Magnetic 96-well automated strip washer
• Color touchscreen makes programming quick and easy
• Easy touch operation for washing full or partial plates
• Reliable and safe: liquid level sensing
• Automated switching of up to 3 buffers for even greater
automation
• Automated, built-in maintenance routines for continued
reliable operation
Replaces the BioTek® ELx50 Magnetic 96-well Strip Washer

BioTek® 50 TS Plate Washer 40-301
38 | sigmaaldrich.com/elisa
Look Closer
All biomarker kits are not
created equal
With assay performance data included in every
protocol, you can discover with confidence.
Rigorous Assay Development Reproducible Results

you can Trust
Specificity
Stability
• Antibody specificity is tested to ensure
negligible cross-reactivity in the tested • All kits are rigorously tested for shipping
sample types stability to determine impact on each analyte
• Assay specificity is ensured by confirming • Sample stability is examined at a range of
consistent performance in singleplex and temperatures, and by freeze/thaw
multiplex formats
Precision and Accuracy
Method Comparisons
• Intra-assay and inter-assay CVs are typically
• We test our kits against in-house kits and kits within 10-15%
from other vendors when available, ensuring
the highest performing kits • Accuracy is normally within ± 20%
Selectivity Linearity of Dilution
• Assay buffers and bead diluents are optimized • Spike recovery in sample is performed for
to enhance antibody binding to only those each analyte at three concentrations; diluted
analytes of interest in samples sample results must be directly proportional
to the concentration of analyte in the sample,
typically within ± 20%
Detection and Sensitivity
• The assay range and minimum detectible Lot-to-Lot Consistency
concentrations (minDCs or LLOQ) for each
analyte is published in our protocols, and is • A “gold standard” calibrator is used as a
based on actual samples reference for all future production lots
Performance in a Sample Matrix Dedicated Support
•A
ssays are verified for the sample type noted • Committed care when and where you need it,
in the protocol; for serum/plasma sample from our sales specialists, technical support
kits, we always provide an optimized native scientists, customer support, and custom
serum matrix to mimic the environment of the immunoassay development team
sample, normalizing assay performance
We’ll help you choose the right platform and assays to meet your needs today, and we’ll
partner with you to meet those needs in the future—without compromising quality.
MilliporeSigma
400 Summit Drive
Burlington, MA 01803
sigmaaldrich.com
To place an order or receive technical assistance

In the U.S. and Canada, call toll-free 1-800-645-5476
For other countries across Europe and the world, please visit: sigmaaldrich.com/offices
For Technical Service, please visit: sigmaaldrich.com/techservice
© 2019 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. MilliporeSigma, the
Vibrant M, MILLIPLEX, MAPmate, SMC and SMCxPRO are trademarks of Merck KGaA, Darmstadt, Germany
or its affiliates. All other trademarks are the property of their respective owners. Detailed information on
trademarks is available via publicly accessible resources.
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• MILLIPLEX® map Assays

The life science business

We’re here to guide you to choose the best protein detection

MILLIPLEX® map Multiplex Detection

SMC™ Assays for SMCxPRO™ or Erenna® Instruments

Flexibility and sensitivity: our platforms fit your purpose

SMCxPRO™ or Erenna® system

Plate reader compatibility

NEW! MILLIPLEX® map and Single Molecule Counting (SMC™) Kits

New SMC™ Streamlined Kit Improvements:

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Analyte Page Analyte Page Analyte Page

4–6 BAX (total)........................................18 Complement Factor B ........................10

α-1 Microglobulin...............................12 BMP-9...............................................12 CREB (total) ............................... 18, 20

Angiopoietin-2............................. 12, 14 CD44 ...............................................12 Eotaxin/CCL11 .........................9, 13, 16

FGF-21 .............................10, 11, 14, 15 IFNβ ................................................. 9 IL-31 ........................................... 9, 13

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

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MDH1 ..............................................12 p38/SAPK2A/B (Thr180/Tyr182) Sclerostin (SOST) .................. 11, 14, 16

MIG/CXCL9 ................................... 9, 13 PARK5/UCHL1...................................12 SMAD4 (total)...................................19

NCAM (soluble)..................................12 PSA (free) ........................................11 Tau (Thr181) ....................................12

TPO .................................................. 9 U VEGFR1/Flt-1 (soluble)............. 10, 12, 14

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

10X Wash Buffer

4 CGTMAG-98K....................16 HKI6MAG-99K...................12 MKI2MAG-94K..................15

46-654MAG......................21 F HNCSMAG-35K..................12 MSHMAG-21K

48-631MAG......................19 HCVD2MAG-67K................11 HTMP2MAG-54K................10 RKTX1MAG-37K................16

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Legend key for MILLIPLEX® map kits

Human Soluble Cytokine Multi-Species TGFβ – 3 Plex ▼ Human IgE – Singleplex

sCD30 sIL-6R Metabolism/

IL-1α MMP-8 GLP-1 (active) ◗ Leptin

Human TIMP Panel 1

TIMP-1 TIMP-2 Amylin (active) † ◗ IL-6

Cortisol ^ Involucrin Human Myokine

IGF-1 IGF-2 Human CVD Panel 5 CD86/B7-2 PD-L2

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Legend key for MILLIPLEX® map kits

Human Circulating Cancer Neuroscience Human Neuropeptide ■ ^

DKK1 Osteoprotegerin Human Kidney Injury Panel 2

GDF-15 (OPG) (Urine samples)

G-CSF VEGF-D Human Neurodegenerative (Cat. No. HKI4MAG-99K)

Human Neurological β-2-Microglobulin Cystatin C

(Cat. No. HND3MAG-39K)

Angiotensinogen Osteopontin (OPN) Human Liver Injury

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Legend key for MILLIPLEX® map kits

(Cat. No. MMMP2MAG-79K)

Mouse MMP Panel 3 Adiponectin

Protein 1 (FSTL1) Musclin

Mouse Neuropeptide ■ ^ Multi-Species TGFβ – 3 Plex ▼

Multi-Species Hormone ■ ^

IP-10/CXCL10 TIMP-1 (Cat. No. RADPKMAG-80K)

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Rat Vascular Injury Panel 3

▼ Premix panel only ■ Requires sample extraction α-1-Acid Glycoprotein Fibrinogen ▲

Bone Canine Cytokine/Chemokine Multi-Species TGFβ – 3 Plex ▼

19 (Cat. No. FCYTMAG-20K-PMX) Cortisol Testosterone

13 (Cat. No. PCYTMG-23K-13PX)

OTHER SPECIES IFNγ

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Analyte Total Phosphorylated Analyte Total Phosphorylated

p70S6 Kinase 3 (Thr389/412) H, M, R p70S6 Kinase 3 H, M, R

• The performance of a larger robot for a fraction of the