4 g of powder extracted from plant will be separated in 100 mL of 0.

2 mol L−1 HCl and 200 mL

of ethanol respectively, at room temperature. The mixture of dye will be prepared by mixing

separately ethanol extracted from a solution of 0.2 mol L−1 HCl to a ratio of 1:1 by volume. The

glass containers will be covered with aluminium foils to prevent damage from light contact and

will be left to shake for one day. Therefore the solid residues will be filtered out to obtain clear

dye solutions