GRAM NEGATIVE BACILLI  H2S indicator: FeSO4 – black ; gas – splits

ENTEROBACTERIACEAE medium
- Gram negative rods TSI Reactions
- Motile with peritrichous flagella A/A: 2-3 sugars fermented A – Acid – Yellow
o Except Klebsiella, Shigella K/A: glucose fermented K – Alkaline – Red
- Facultative anaerobe K/K: NO sugar fermented
- Ferments GLUCOSE Standard Inoculation: 18-24 hours
- Often produce gas
- Catalase POSITIVE LSI (Lysine Iron Agar)
o Except Shigella dysenteriae To detect if the bacteria has the ability to degrade amino
- Oxidase NEGATIVE acid lysine
o Except Vibrio pH indicator: Bromcresol purple
- Reduce nitrate > nitrite H2S indicator: Ferric Ammonium Citrate – Black
o Except Erwina, Pantoea agglomerans Lysine decarboxylation: BUTT
(+) Purple (-) Yellow
H2S Producers Lysine deamination: SLANT
(+) Blackening of the medium (+) Red (-) Purple
- Shigella LIA Reactions
- Proteus K/K: (+) LDC Acidic - Yellow
- Providencia K/A (-) LDC Alkaline - Purple
- Arizona R/A (+) LDC
- Citrobacter freundii
- Edwardsiella IMViC TEST
-
TSI (Triple Sugar Iron) Indole (+) Red ring
 CHO: Sucrose, Lactose, Glucose
 Ratio: 10:10:1 resp. + Iron Detects: Tryptophanase
► Na thiosulfate and Ferrous sulfate – responsible for Substrate: Tryptophan
hydrogen sulfide production detection. Medium: SIM Medium, Tryptophan broth / slant
 pH indicator: phenol red (red – yellow) Indicator: Kavac’s reagent / Ehrlich reagent / PDAB