Nursing Biochemistry

Laboratory Report

Carbohydrates partII:
specific Reaction
Activity No. 4

Dela Cruz, Juan C. (Principal Author)

Palanggana, Maria Patricia D.
Sumamasa, Iba L.
Locker No.1; NurBio Lab E
Nursing Biochemistry
Laboratory Report

1st Semester, SY 2019-20

RATIONALE
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laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation
ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat.

DATA INTERPRETATION

Mucic acids:

Seliwanoff’s Test:

Seliwanoff’s Test distinguishes between aldose and ketose sugars. Ketoses are distinguished from aldoses
via their ketone/aldehyde functionality. If the sugar contains a ketone group, it is a ketose and if it
contains an aldehyde group, it is an aldose. This test is based on the fact that, when heated, ketoses are
more rapidly dehydrated than aldoses. Perform this test with glucose, fructose, maltose and sucrose.

The specific chemical equation for this specific event shown the below:

Figure.1 for the seliwanoff’s test

Bial’s Orcinol Test:

Bial’s Test is to determine the presence of pentoses (5C sugars). The components of this reagent are
resorcinol, HCl, and ferric chloride. In this test, the pentose is dehydrated to form furfural and the solution
turns bluish and a precipitate may form. Perform this test with ribose and glucose. The experiment will

____________________________________________________________________________________________________ 1
Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga University
Nursing Biochemistry
Laboratory Report

distinguished between the hexose and pentose. They react with the bials reagent and are converted to the
furfural presence of the substance.

This is the specific chemical composition and the form of the chemical test of the Bial’s:

Iodine Test:

The test is used for the detection of the starch in the solution. The blue black color is due to the formation
of the starch iodine complex. Starch contains polymers of α-amylose and amylopectin which form a
complex with iodine to give the color blue black color.Iodine test is an indicator for the presence of
starch. Iodine solution (iodine dissolved in an aqueous solution of potassium iodide) reacts with starch
producing a blue-black color. Apply this test to all the polysaccharides provided.

Table No. 1

Name of test Test solution Results Explanation

Mucic acids (theoretical result) Galactose

Glusoce

Lactose

Unknown

Seliwanoff’s Test %Fructose

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Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga University
Nursing Biochemistry
Laboratory Report

%Glucosse

%Sucrose

% Maltose

Unknown
sugar

Bial’s Orcinol Test %xylose

%glucose

%lactose

Unknown
sugar

Test solution Time of Formation Color Shape Sketch

%glucose

%fructose

%galactose

%lactose

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Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga University
Nursing Biochemistry
Laboratory Report

%maltose

%sucrose

% arabinose

Unknown sugar

ANSWERS TO QUESTIONS

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quod mazim placerat facer possim assum. Typi non habent claritatem insitam; est usus legentis in
iis qui facit eorum claritatem. Investigationes demonstraverunt lectores legere me lius quod ii
legunt saepius?

ANSWER: kvesjajcj . Typi non habent claritatem insitam; est usus legentis in iis qui facit
eorum claritatem. Investigationes demonstraverunt lectores legere me lius quod ii legunt
saepius.

2. Typi non habent claritatem insitam; est usus legentis in iis qui facit eorum claritatem.
Investigationes demonstraverunt lectores legere me lius quod ii legunt saepius?

ANSWER: . Typi non habent claritatem insitam; est usus legentis in iis qui facit eorum
claritatem. Investigationes demonstraverunt lectores legere me lius quod ii legunt saepius.

3. Etc.

CONCLUSION

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Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga University
Nursing Biochemistry
Laboratory Report

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laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation
ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat.

REFERENCES

Use APA format.

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Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga University