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GROUP 1
BSMD4Y1-2
1. Major drawback of frozen section immunofluorescence.
A. Tissue
B. Fluorescence microscope
C. Considerable skills
D. Fades within days after immunostaining
Answer: B. All of the choices are disadvantages of immunofluorescence. But the most common
disadvantage of this technique is the FLUORESCENCE MICROSCOPE because of its expensive price.
Answer: C. Between each step of the staining technique, sections require several brief washing with TBS
to prevent contamination.
4. Standard method among most laboratories, in combination with frozen section processing for
immunofluorescence of renal and skin biopsies.
A. Paraffin Wax Section Immunoperoxidase Technique
B. Frozen Section Immunocytochemistry
C. Frozen Section Immunofluorescence
D. NOTA
Answer: A. Paraffin Wax Section Immunoperoxidase Technique remains a standard method because
paraffin-embedded specimens combine good morphology with localization of various cell and tissue
markers.
5. Temperature requirement in preparing paraffin wax sections for immunostaining if sections are
without adhesives.
A. 60°C
B. 24°C
C. 0°C
ANGELES, JOHN EDUARD A.
GROUP 1
BSMD4Y1-2
D. -10°C
Answer: A. Place sections at 60°C microwave oven overnight if processing sections without adhesives.
Answer: B. Cell Surface Antigens are best preserved in sections fixed briefly in COLD ACETONE.
7. Dilution of normal swine serum in preparing PAP Technique for Paraffin Section.
A. 1:7
B. 1:2
C. 1:10
D. 5:5
Answer: C. Incubate with 1:10 dilution of normal swine serum in preparing PAP technique.
Answer: The traditional direct technique of doing immunohistochemistry is to conjugate the primary
antibody directly to the label, such as FLUOROCHROME and HORSERADISH PEROXIDASE.
Answer: A. Misinterpretation resulting from false-positive reactions can be avoided by using ANTI-
ALBUMIN CONTROL.
10. Factor/s that will reduce the likelihood of weak staining due to dissociation of already formed
immune complex.
A. Low salt concentration
B. Monoclonal antibodies
C. Low temperature
ANGELES, JOHN EDUARD A.
GROUP 1
BSMD4Y1-2
D. Bot A and C
Answer: D. Low salt concentration as well as low temperatures will reduce the likelihood of weak
staining due to dissociation of already formed immune compleslx.