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ANGELES, JOHN EDUARD A.

GROUP 1
BSMD4Y1-2
1. Major drawback of frozen section immunofluorescence.
A. Tissue
B. Fluorescence microscope
C. Considerable skills
D. Fades within days after immunostaining

Answer: B. All of the choices are disadvantages of immunofluorescence. But the most common
disadvantage of this technique is the FLUORESCENCE MICROSCOPE because of its expensive price.

2. Desired thickness for frozen section immunocytochemistry.


A. 1.0x1.0x0.3cm
B. 1.1x1.1x1.1cm
C. 0.3x0.3x0.1cm
D. 0.45cm

Answer: A. A 1.0x1.0x0.3cm block of tissue can be snap frozen.

3. What step is done to prevent one reagent contaminating another?


A. Fix with alcohol
B. Clearing with xylene
C. Washing with TBS
D. Drying

Answer: C. Between each step of the staining technique, sections require several brief washing with TBS
to prevent contamination.

4. Standard method among most laboratories, in combination with frozen section processing for
immunofluorescence of renal and skin biopsies.
A. Paraffin Wax Section Immunoperoxidase Technique
B. Frozen Section Immunocytochemistry
C. Frozen Section Immunofluorescence
D. NOTA

Answer: A. Paraffin Wax Section Immunoperoxidase Technique remains a standard method because
paraffin-embedded specimens combine good morphology with localization of various cell and tissue
markers.

5. Temperature requirement in preparing paraffin wax sections for immunostaining if sections are
without adhesives.
A. 60°C
B. 24°C
C. 0°C
ANGELES, JOHN EDUARD A.
GROUP 1
BSMD4Y1-2
D. -10°C

Answer: A. Place sections at 60°C microwave oven overnight if processing sections without adhesives.

6. Best fixative to preserved cell surface antigens.


A. Acetone
B. Cold Acetone
C. B5
D. TBS

Answer: B. Cell Surface Antigens are best preserved in sections fixed briefly in COLD ACETONE.

7. Dilution of normal swine serum in preparing PAP Technique for Paraffin Section.
A. 1:7
B. 1:2
C. 1:10
D. 5:5

Answer: C. Incubate with 1:10 dilution of normal swine serum in preparing PAP technique.

8. What are the 2 labels used in Direct Technique?


- Fluorochrome
- Horseradish peroxidase

Answer: The traditional direct technique of doing immunohistochemistry is to conjugate the primary
antibody directly to the label, such as FLUOROCHROME and HORSERADISH PEROXIDASE.

9. How to avoid misinterpretation resulting from false-positive reactions?


A. Anti-albumin control
B. Triton X
C. Reheating
D. None

Answer: A. Misinterpretation resulting from false-positive reactions can be avoided by using ANTI-
ALBUMIN CONTROL.

10. Factor/s that will reduce the likelihood of weak staining due to dissociation of already formed
immune complex.
A. Low salt concentration
B. Monoclonal antibodies
C. Low temperature
ANGELES, JOHN EDUARD A.
GROUP 1
BSMD4Y1-2
D. Bot A and C

Answer: D. Low salt concentration as well as low temperatures will reduce the likelihood of weak
staining due to dissociation of already formed immune compleslx.

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