Sln Vainillina (40 Solución Solución

ug/mL) monitor PATRÓN NaOH Volumen

stock (mL) 0.1M para final
diluir (mL) (mL) abs _354_____
0 (blanco) 10 0
1 0.25 ug/mL 0,5 ml 1,5 ml 10 0.003
2 0.5 ug/mL 1 ml 9 ML 10 0.007
3 1 ug/mL 2 ml 8 ML 10 0.01
4 2 ug/mL 4 ml 6 ml 10 0.017
5 3 ug/mL 6 ml 4 ml 10 0.022
6 4 ug/mL 8 ml 2 ml 10 0.029
7 5 ug/mL (2°patrón) 10 ml 0 ml 50 0.035
8 10 ug/mL 10ml 0 ml 50 0.06
9 20 ug/mL 10 ml 0 ml 50 0.113

grafica 1
0.12
77.2 0.7

0.1 0.6

0.08 0.5

0.06 87.3 0.4

0.3
0.04
92.2
93 0.2
0.02 95
96.2
97.8 0.1
98.4
0 100 99.6
99.6
0 2 4 6 8 10 12 0 100
0 2
tramitansa abs _408___ tramitansa
100 0 100
99.6 0.03 99.6
98.4 0.07 98.4
97.8 0.1 97.8
96.2 0.17 96.2
95 0.22 95
93 0.29 93
92.2 0.35 92.2
87.3 0.6 87.3
77.2 0.1 77.2

grafica 2
0.7

0.6 87.3

0.5

0.4
92.2
0.3 93

0.2 95
96.2
0.1 97.8 77.2
98.4
99.6
0 100
0 2 4 6 8 10 12