APPLICATION

BRIEF

1: QUANTITATION 2: QUANTITATION
OF AFLATOXIN (B1, B2, G1, G2) IN SPICES OF AFLATOXIN (B1, B2, G1, G2) IN GRAINS
Method: HPLC-FLD Method: HPLC-FLD
IAC column: CHROMAtific Aflatoxin column, 3mL (p/n: AFLA3) IAC column: CHROMAtific Aflatoxin column, 3mL (p/n: AFLA3)

Grind and weigh 10g sample Grind and weigh 25g sample

Add 80ml 90+10 AcN+H2O, blend Add 100mL 60+40 MeOH+H2O, blend
for 3 minutes, or shake for 90 minutes for 3 minutes, or shake for 60 minutes

Filter supernatant Filter supernatant

Mix 2ml of extract with 16ml of PBS

Mix 4mL of extract with 12ml of PBS
(containing 0.4% Tween)

Apply to IAC column (p/n: AFL3) Apply to IAC column (p/n: AFL3)

Wash column with 3 x 3.3ml distilled water Wash column with 2 x 10mL distilled water

Elute column with 3x0.5ml AcN Elute column with 3x0.5ml MeOH

Inject 20μl of the eluate Inject or evaporate and re-dissolve

1 CHROMATIFIC.DE
INFO@CHROMATIFIC.DE
 APPLICATION
BRIEF

3: QUANTITATION 4: QUANTITATION
OF AFLATOXIN (B1, B2, G1, G2) IN HAY OF AFLATOXIN (B1, B2, G1, G2) IN NUTS
Method: HPLC-FLD Method: HPLC-FLD
IAC column: CHROMAtific Aflatoxin column, 3mL (p/n: AFLA3) IAC column: CHROMAtific Aflatoxin column, 3mL (p/n: AFLA3)

Grind and weigh 12.5g sample Grind and weigh 25g sample

Add 100mL 60+40 MeOH+H2O, blend Add 100mL 60+40 MeOH+H2O, blend
for 3 minutes, or shake for 60 minutes for 3 minutes, or shake for 60 minutes

Filter supernatant Filter supernatant

Mix 4mL of extract with 12ml of PBS Mix 4mL of extract with 12ml of PBS

Apply to IAC column (p/n: AFL3) Apply to IAC column (p/n: AFL3)

Wash column with 2 x 10mL distilled water Wash column with 2 x 10mL distilled water

Elute column with 3x0.5ml MeOH Elute column with 3x0.5ml MeOH

Inject or evaporate and re-dissolve Inject or evaporate and re-dissolve

2 CHROMATIFIC.DE
INFO@CHROMATIFIC.DE