Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
Publishing
rsc.org/…/PDMS_connector.asp 1/2
11/8/2010 PDMS macro-micro Generated by Foxit PDF Creator © Foxit Software
connectors
http://www.foxitsoftware.com For evaluation only.
3. Place the filter/adhesive into the lid of the Petri dish. Make sure the
filter paper faces up and that the whole filter/adhesive assembly lies flat
against the dish. Do not worry if the edge of the filter/adhesive curls up
a little on the side. This will result in thinner PDMS connectors on that
side.
They will still be functional. Mix 25 g of
PDMS with 2.5 g (10%) hardening
agent and pour it into the dish (Figure
Figure 1 2). Place on a hot plate at 50 degrees
Celsius and cure for 4 h.
4. Remove the dish from the hot plate and let cool for 5 min. Using the
knife cut the PDMS around the edges of the dish to loosen it. Then pry
the PDMS from the dish (Figure 3).
Figure 2
5. Take the 16 gauge blunt needle and
place it on the surface of the PDMS. Turn the needle back and forth
while applying pressure to create a through-hole. Make several holes
in a row (Figure 4).
6. Take the knife and cut squares
around each of the holes. After cutting
out the row, each hole can then be
separated into individual connectors
Figure 3 (Figure 5).
7. Clean the surface of your microfluidic
device with some ethanol. (The surface
must be clean for good adhesion.) Peel
the adhesive off the bottom of the Figure 4
PDMS connector. This should remove
the PDMS core made with the blunt needle. If not, you may need to
remove it with fine tweezers.
8. Attach the connector to your microfluidic device. Insert tubing into
Figure 5 the connector and attach the other end to a fluidic source such as a
syringe (Figure 6).
Downloadable Files
Circle Template
PDF (417k)
PDMS Connectors figures
PDF (8641k) Figure 6
rsc.org/…/PDMS_connector.asp 2/2