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Identify the
freezing point
of the isotonic solution

Understand how
Determine the use
freezing point is
of freezing point
a colligative
in physiologic
property
applications

GROUP 3
objectives 2
Physical properties of SOLUTION

HOMOGENUOS MIXTURE
COMPOSE OF SOLUTE AND SOLVENT

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COAGULATIVE PROPERTIES

DEPEND on the CONCENTRATION of solutes

NOT on the IDENTITY of the solute

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 COAGULATIVE PROPERTIES

1. VAPOR PRESSURE LOWERING

2. BOILING POINT ELEVATION

3. FREEZING POINT DEPRESSION

4. OSMOTIC PRESSURE
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 FREEZING POINT DEPRESSION

Is the difference in the melting points of pure water and a solution

There is a decrease in
temperature
for the freezing point of the
solution compared to pure water.
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Salt in a water solution disrupts molecules to form ice

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Physiological
applications
 a solution that has higher solute
concentration than another
solution

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 A solution that has a lower
solute concentration than
another solution

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 - a solution that has the same solute
concentration as another solution
- have colligative properties similar to
body fluids
- contain solutes that are not capable
of diffusing through biological
membranes
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 Sodium chloride TEST tube
USP Provided with perforated cork to fit
thermometer
Distilled water
Ice cubes
Thermometer With a small amount of table salt
With zero calibrations

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 4. Fill another beaker
with ice cubes enough
to immense the test
1. Weigh 0.18 g NaCl,
tube
USP
Clamp the test tube on
an iron stand,
2. Dissolve in a beaker
accurately during the
to make 20 mL
whole process
3. Pour 5 mL of solution
in a test tube, Fit in the Allow the solution to
cork with thermometer freeze, Record
Temperature
 thermometer
Stirring rod

Sample tube
clamps

BEAKER WITH ICE

AND SALT
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