Monitoring exposure to

Lovibond Colour Instruments

Organic Phosphorus Insecticides

Rapid , accurate
field-testing

Lovibond AF267 Cholinesterase kit

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 L ov i b o n d ® A F 2 6 7 C h o l i n e s t e r a s e t e s t k i t
Rapid, accurate field testing
Lovibond Colour Instruments

The Lovibond® Cholinesterase Test Kit is designed for rapid field-testing of exposure to organic phosphorus insecticide and to alert
users of these chemicals (farmers, spray operators, research workers) to their absorption and unsafe working practices. The kit is
specified by the World Health Organisation in its technical information sheet No 356, 1967. It is particularly useful for emergency use
or away from the laboratory.

The absorption of organic phosphorus insecticides is accompanied by reduced levels of cholinesterase enzyme activity in the blood,
which, at normal levels, will keep muscles, nerves and glands functioning properly. Therefore, a measure of cholinesterase activity in
the blood as a percentage of the activity in normal blood serves as an indication of the seriousness of exposure and potential danger
to health. Reduced levels of cholinesterase enzyme activity can cause twitching of muscles and excessive secretion of tears and saliva;
weak breathing and feeble heartbeat may also be encountered.

The AF 267 test kit uses simple equipment and a straightforward colorimetric test method to give rapid results of sufficient accuracy
without the need for medical or clinical expertise. A small blood sample is taken from the person being tested for exposure to the
insecticides; following the addition of reagents, which develop a colour in the sample ranging from green to yellow that is dependent
on the level of cholinesterase activity, the sample is visually matched with glass colour standards in a test disc using the Lovibond®
Comparator 2000+. Each glass colour standard represents a percentage of normal cholinesterase activity, ranging from 0 - 100% in
steps of 12.5%. The results are analysed according to a simple table, indicating the action that should be taken if over-exposure is
found.

Contents The kit is supplied in a rigid, wooden carrying case and contains all reagents and apparatus necessary for field-testing as well as full
instructions for carrying out the test:
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Lovibond® Comparator 2000+ Polythene wash bottle (250 ml)

Cholinesterase disc 5/30, 0 - 100% of normal cholinesterase activity Polythene volumetric flask (250 ml)
3 precision optical glass cells (2.5 mm) Box of 100 disposable swabs
20 glass test tubes with rubber bungs Box of 200 disposable blood lancets
Test tube rack with built-in thermometer Glass stirring rod
10 ml automatic pipette Test tube brush
1000 disposable pipette tips Surgical gloves
Glass beaker (250 ml) Spatula
3 glass bottles with calibrated pipette (60 ml) 4 ampoules of bromothymol blue powder (0.112 gm)
Polythene measuring cylinder (50 ml) Bottle of acetylcholine perchlorate powder (25 gm) *
Polythene bottle (500 ml) Step-by-step instruction leaflet

* Transport restrictions may make it preferable for local supply of acetylcholine perchlorate powder. Please enquire when ordering
O rder Code 41 26 70

The Tintometer Ltd • Lovibond House • Solar Way • Solstice Park • Amesbury • SP4 7SZ • UK
Tel: +44 (0)1980 664800 • Fax: +44 (0)1980 625412 • Email: sales@tintometer.com • Website: www.tintometer.com
Lovibond® & Tintometer® are Registered Trade Marks of The Tintometer Limited. All translations and transliterations of Lovibond® & Tintometer®
are asserted as Trade Marks of The Tintometer Limited. Registered in England Number 45024. Registered Office: Lovibond House • UK. 920460_V2_05/07