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Postharvest Quality of Coated Cherries cv.

‘Burlat’ as Affected
by Coating Composition and Solids Content

C. Rojas-Argudo*, M.B. Pérez-Gago and M.A. del Río

Departamento de Postcosecha Instituto Valenciano de Investigaciones Agrarias 46113 Moncada, Valencia, Spain

The aim of this work was to study the effect of edible composite coating with different
hydrophobic/hydrophilic ratio and solids content (SC) to improve storability of fresh cherries. Cherries
cv. Burlat were coated with four edible composite coatings based on locust bean gum (LBG), shellac and
beeswax. Three coatings differed on the hydrophobic/hydrophilic ratio (75/25, 50/50 and 25/75) and were
applied at constant SC (1.75%). Whereas, one coating was applied at 3.50% SC, maintaining the
hydrophobic/hydrophilic ratio at 75/25. Quality was assessed during storage at 1 °C up to 11 days fol-
lowed by 1 day at 20 °C. Increasing coating hydrophobicity decreased weight and firmness loss of cher-
ries during storage. Increasing SC did not improve weight and firmness loss, but it increased
deterioration index. In general, no benefits were achieved in colour and titratable acidity retention as a
result of coating application. Major benefits by coating application were found in weight, firmness and
deterioration index. The application of the most hydrophobic solution at 1.75% of SC prolonged the
storability of the cherries.

Key Words: cherries, edible composite coatings, shellac, beeswax, locust bean gum

INTRODUCTION storage life of cherries, along with other quality para-


meters such as loss of brightness, firmness and appear-
There is an increasing consumer interest regarding ance (Drake, 1991). Sweet cherries offer good
more wholesome food and more natural products. This tolerance to high levels of CO2 (Beaudry, 1999), thus
has orientated coatings research towards the develop- modified-atmosphere packaging (MAP) has been used
ment of new waxes, specifically ‘edible coatings’ to to preserve their postharvest quality (André et al.,
improve food appearance and safety. Nowadays it is 1982; Remón et al., 2000). Despite MAP effectiveness,
possible to apply the technology of coatings to fruits its use is associated with problems, such as water con-
that are consumed with skin and that are easily spoiled, densation caused by temperature fluctuations during
for example, cherries. storage and/or transportation, and waste generation
Edible coatings can incorporate proteins, polysac- through the use of plastic materials. Edible coatings
charides and lipids (Baldwin, 1994, 1999). Polysaccha- provide a semi-permeable barrier to gases and water
rides have been used as film forming agents and vapour, which creates a modified atmosphere in the
because of their selective permeabilities to O2 and CO2 fruit and delays dehydration. In addition, from an
(Nisperos-Carriedo, 1994). Polysaccharides commonly environmental perspective, the replacement of plastic
used in edible coatings include cellulose derivatives, materials by edible or biodegradable films is also envi-
starches and chitosan (Nisperos-Carriedo, 1994) and a ronmentally desirable.
few recent studies deal with the use of LBG in edible Even though there is little literature about edible
coatings (Chen and Nussinovitch, 2000, 2001; Albert coatings on cherries, good results have been reported:
and Mittel, 2002). reducing weight loss and surface pitting (Lidster, 1981;
Weight loss is a major factor in determining the Drake et al., 1988; Rojas and del Río, 2001; Rojas et
al., 2003), maintaining texture (Duarte et al., 1999) and
improving postharvest quality in general (Yaman and
*To whom correspondence should be sent Bayindirli, 2002). On the other hand, a slight increase
(e-mail: crojas@ivia.es). in fungal spoilage of edible coated cherries, compared
Received 8 September 2004; revised 7 February 2005. to uncoated ones, was detected by Yaman and
Bayindirli (2001).
Food Sci Tech Int 2005; 11(6):417–424 Burlat is an early cherry cultivar extensively grown in
© 2005 SAGE Publications
ISSN: 1082-0132 Spain, with a very short postharvest life which has been
DOI: 10.1177/1082013205060180 reported to be less than 1 week under conventional
418 C. ROJAS-ARGUDO ET AL.
storage conditions (André et al., 1982). Postharvest materials included beeswax, shellac and polysorbate
technologies commonly applied to cherries, such as pre- 80; hydrophilic materials included LBG and glycerol.
cooling, did not provide good results when applied to cv. Each of the emulsion coatings, HL1, HL2 and HL3,
Burlat due to its high perishability (Martínez-Jávega et was prepared from mixtures of two formulations: one a
al., 1992; Alique and Zamorano, 2000). beeswax-based emulsion and the other a shellac-based
The objective of this work was to study the effect of solution. LBG dispersed in ethanol (4 mL ethanol/g
LBG–lipid composite coatings applied to ‘Burlat’ cher- LBG) was added to hot water at 60 °C. Shellac or
ries using different hydrophobic/hydrophilic ratios and beeswax were added to obtain the beeswax emulsion or
solids content and to evaluate their performance on the shellac solution, respectively. Next, polysorbate 80
maintaining postharvest quality. and glycerol were added to the beeswax-based emulsion
and glycerol was added to the shellac-based solution. To
help in the melting of the wax and resin, solutions were
MATERIALS AND METHODS heated to a temperature of 10 °C to 20 °C above the
melting point of the lipids. The pH was increased by
Materials adding NH3 to disperse the shellac and beeswax. Once
the lipids were melted, samples were homogenised with
LBG (Ceamgum CV50) was supplied by Ceamsa a high-shear probe mixer (PolyTron, Model, IKA) for
(Porriño, Spain). Beeswax (grade 1) and shellac were 2 min at 30,000 rpm. Further cooling was achieved by
purchased from Brillocera, S.A. (Valencia, Spain). placing the emulsions in an ice bath to lower the temper-
Polysorbate 80 (polyoxyethylene sorbitan ature to less than 30 °C. Appropriate quantities of the
monooleate), glycerol and ammonium hydroxide at two formulations were used to get the coating composi-
30% were from Panreac Química, S.A. (Barcelona, tions shown in Table 1. A more detailed procedure can
Spain). be found in Rojas-Argudo (2002). Coatings were
applied at 1.75% SC, additionally, the coating HL1 was
Coating Formulation applied at a higher SC (3.50%), which corresponds to
treatment 2-HL1. Treatments and characteristics of the
Three experimental coatings were formulated con- coatings are summarised in Table 2.
sisting of LBG, beeswax, shellac, polysorbate 80, glyc-
erol, NH3 and water. Glycerol and polysorbate 80 were
Methods
used as plasticiser and emulsifier, respectively. The
experimental coatings differed in the ratios of Sample Preparation and Coating Application
hydrophobic/hydrophilic components and were identi-
fied as HL1, HL2 and HL3. HL1 being the most Burlat cherries were harvested by hand in May and
hydrophobic coating and HL3 the least. Hydrophobic transported from Alpatró (Alicante) to the research

Table 1. Coatings composition.


Coating* (% dry basis)

Component HL1 HL2 HL3

LBG 13.0 26.0 39.0


Glycerol 12.0 24.0 36.0
Shellac 37.5 25.0 12.5
Beeswax 30.0 20.0 10.0
Polysorbate 80 7.5 5.0 2.5

*All coating formulations were prepared by adding 0.5 g NH3 (30%) per 1 g of shellac and per 1 g of beeswax.

Table 2. Treatments applied to Burlat cherries.


Treatment Hydrophobic/Hydrophilic Ratio* SC (%)

HL1 75/25 1.75


HL2 50/50 1.75
HL3 25/75 1.75
2-HL1 75/25 3.5
Uncoated – –

*Hydrophobic materials included beeswax, shellac and polysorbate 80. Hydrophilic materials included LBG and glycerol.
Postharvest Quality of Coated Cherries cv. ‘Burlat’ 419
laboratory the same day. They were stored overnight experience tasting fruits. Panellists rated flavour on a
at 5 °C. The next day, after fruit conditioning at 20 °C, 9-point scale: 1  very poor, 5  fair and 9  optimum.
cherries were selected for size, colour and absence of Samples were presented to panellists in trays labelled
physical damage, and coatings were applied. Cherries with 3-digit random codes and served at room temper-
were dip-coated by immersion in the coating solutions ature (22  1 °C). Spring water was provided for oral
for 20 s, drained of excess coating, and air-dried at rinsing between samples.
room temperature with a fan having an air flow of
7,500 m3/h. Ethanol and Acetaldehyde Content
After coating, cherries were stored at 1 °C and
90–95% RH. Fruit analysis was carried out after 6 and Samples prepared as described above were used to
11 days of storage at 1 °C, followed by 1 day of storage determine ethanol content (EC) and acetaldehyde
at 20 °C to simulate retail storage. content (AC). The method to measure EC and AC in
the juice was slightly modified from that of Davis and
Weight Loss Chace (1969). Five mL of juice was each transferred to a
10-mL vial with crimp-top cap and TFE/silicon septum
Individual weight loss in 30 fruits were registered seal and frozen (20°C) until analysed. For the analysis,
and expressed as the percentage loss of initial weight. samples were put in a water bath at 20°C for 1h and then
heated at 60°C for 10min in a water bath to reach equi-
Postharvest Quality librium in the headspace. A 1-mL sample of the head-
space was withdrawn from a vial and injected into a gas
Quality Parameters chromatograph (model 1020; Perkin Elmer Corp.,
Norwalk, Conn., USA) fitted with a Porapack QS 80/100
Fruit firmness was determined on 20 fruits per treat- (1.2m0.32cm i.d.) column and a flame ionisation
ment with a Universal Testing Machine (Model 4301; detector. Helium was the carrier gas, set at 12.3psi. Injec-
Instron Corp., Canton, Mass., USA) as the force (kg) tor, oven and detector temperatures were 175°C, 150°C
required to deform 3 mm using a 35-mm-diameter and 200°C, respectively. Ethanol and acetaldehyde were
plunger at a speed of 5 mm/min. identified by comparison of retention times with stand-
Peel colour was measured with a Minolta Chro- ards. Results were expressed as mg/100mL juice.
mameter CR-300 (Minolta Co. Ltd, Osaka, Japan) with
an 8-mm-diameter measuring area, using illuminant C
Deterioration Index
and 2° observer. Two measurements in the equatorial
area of the fruit were taken, using 50 fruits per treat- Physiological disorders and rotted cherries were
ment. The Hunter L a,b colour system was used and evaluated in 100 fruits per treatment. The different
lightness (L), chroma (C  (a2  b2)1/2) and hue angle degrees of physiological disorders, which included
(h  tan1b/a) were calculated to study colour evolu- bruising injury and surface pitting, were rated as
tion. 0  none, 1  light, 2  moderate and 3  severe. Light
Hunter colour difference (E) was calculated from was considered when disorder affected less than 25%
the equation E  (L2  a2  b2)1/2 using as refer- of fruit surface and severe when more than 50% of
ence cherry initial colour values measured before fruit surface was affected or fruit was rotted. Results
storage. were converted to an average index (0–3).
Three replicates per treatment consisting of juice
from 50 pitted cherries each were used to determine
Statistical Analysis
soluble solids content (SSC) and titratable acidity
(TA). Measurements were made with two repeated Statistical analysis was performed using STAT-
observations per replicate. SSC was measured with a GRAPHICS Plus 2.1 (Manugistics, Inc., Rockville,
digital refractometer (model PR1, Atago, Tokyo, Md., USA). Significant differences between means
Japan) and expressed as °Brix. TA was measured by were determined by least significant difference (LSD)
titration with NaOH up to pH 8.1 (phenolphthalein at 5% level of significance (p  0.05).
turning point) and expressed as g of malic acid/100 mL
of juice. Maturity index (MI) was calculated as the
°Brix/TA ratio.
RESULTS AND DISCUSSION
Flavour Evaluation Weight Loss

The effect of the coatings on the sensory quality of Water loss due to transpiration is the cause of
the samples, initially and after each storage period, was almost all postharvest weight loss of cherries during
assessed by 8 to 10 judges, each with several years of storage (Drake, 1991). Lipids and resins are added to
420 C. ROJAS-ARGUDO ET AL.
coating formulations to impart hydrophobicity, thus to amount of coating is increased, but it can cause an
reduce water loss. Lipids include natural waxes such as excessive modification of the internal atmosphere com-
carnauba wax, candelilla wax and beeswax. Resins, such position that might induce anaerobiosis.
as shellac, wood rosin and coumarone indene are also
used to impart gloss (Baldwin, 1994). In our work,
Postharvest Quality
weight loss of coated cherries decreased as the
hydrophobic content of the coatings increased (Table 3). Quality Parameters
Not all the coating treatments were effective at reducing
weight loss compared to the control. Weight loss of Firmness was measured as the resistance force
cherries coated with the most hydrophilic coating (HL3) required to deform the cherries 3 mm. After 6 days of
was higher than weight loss of uncoated cherries, prob- storage at 1 °C plus 1 day at 20 °C, firmness increased in
ably due to a partial removal of the natural wax layer of all treatments compared to firmness at harvest (Table
the cuticle when dipping the cherries in the coating solu- 4). Other authors have also described an initial
tion together with an increase in handling during the increase in firmness after cold storage of cherries
coating application. Therefore, these results indicate the (Chen et al., 1981; Meheriuk et al., 1997; Remón et al.,
need to control the hydrophobic : hydrophilic ratio of the 2000). After this cold storage, uncoated cherries and
coatings applied to cherries. cherries coated with the most hydrophilic coating
Weight loss of cherries did not decrease as SC (HL3) were the least firm. An increase in SC from
increased from treatment HL1 to 2-HL1 (Table 3). 1.75% (HL1) to 3.5% (2-HL1) did not affect firmness.
Amarante et al. (2001) found in Comice pears coated Some authors have correlated firmness and weight
with dilutions of a commercial carnauba-based coating loss in cherries (Patten and Patterson, 1985). In our
that a little coating deposit was able to coat cuticle work, after 11 days of storage at 1 °C plus 1 day at
imperfections and that a further deposit of coating 20 °C, cherries coated with the most hydrophilic
exerted little effect on reducing water vapour perme- coating (HL3) showed the lowest firmness, which cor-
ability and, consequently, weight loss. Pérez-Gago et relates with the higher weight loss of these cherries.
al. (2003) did not observe SC influence on weight loss Cherry darkening during storage provoked a loss of
of mandarin oranges coated with hydroxypropyl lightness. Hue angle is an indicator of cherry ripeness
methylcelullose-beeswax edible composite coatings (Yaman and Bayindirli, 2002) and lower chroma values
having 4% and 8% SC. Amarante and Banks (2001) indicate less vivid colours. Rojas-Argudo (2002) did
discussed the potential of minimising coating deposit, not observe a decrease in hue angle of Burlat cherries
since small beneficial effects are expected as the stored 2 weeks at 1 °C plus 1 day at 20 °C but detected

Table 3. Effect of LBG-based coatings on weight loss of Burlat cherries.


Weight Loss at Storage (%)

Treatment* 6 days at 1 °C  1 day at 20 °C 11 days at 1 °C  1 day at 20 °C

HL1 9.0 a 16.8 a


HL2 10.6 b 21.8 b
HL3 13.8 c 25.1 c
2-HL1 9.8 a 16.4 a
Uncoated 11.4 b 21.2 b

*Hydrophobicity of the coatings decreased from HL1 to HL3. Solids content of 2-HL1 coating doubled those of HL1 coating. Means within each storage time with
different letter are significantly different at p  0.05.

Table 4. Effect of LBG-based coatings on firmness of Burlat cherries.


Firmness at Storage* (kg)

Treatment 6 days at 1 °C  1 day at 20 °C 11 days at 1 °C  1 day at 20 °C

HL1 0.416 b 0.300 bc


HL2 0.436 b 0.354 c
HL3 0.347 a 0.170 a
2-HL1 0.437 b 0.295 bc
Uncoated 0.393 ab 0.256 b

*Firmness at harvest: 0.320 kg. Firmness measured as force to deform 3 mm. Hydrophobicity of the coatings decreased from HL1 to HL3. Solids content of 2-HL1
coating was double that of HL1 coating. Means within each storage time with different letter are significantly different at p  0.05.
Postharvest Quality of Coated Cherries cv. ‘Burlat’ 421

Table 5. Effect of LBG-based coatings on Hunter colour indexes (L, c and h) and colour differences (E) during
storage of Burlat cherries.
Colour parameters at storage*

6 days at 1 °C  1 day at 20 °C 11 days at 1 °C  1 day at 20 °C

Treatment** L c h E L c h E

HL1 25.2 c 16.0 b 14.6 b 6.75 a 22.6 a 12.5 b 12.6 ab 9.22 a


HL2 24.2 b 15.4 b 13.9 b 7.02 ab 22.2 a 13.3 b 12.7 ab 9.14 a
HL3 22.8 a 11.1 a 12.4 a 9.42 c 22.2 a 10.0 a 11.8 a 10.91 b
2-HL1 25.1 c 11.9 a 13.8 ab 8.40 bc 23.8 b 13.0 b 13.9 c 8.49 a
Uncoated 24.8 bc 10.2 a 16.5 c 9.23 c 23.5 b 10.3 a 12.9 bc 9.61 ab

*At harvest: L  28.5, c  18, hue  15.9°. Means within each storage time with different letter are significantly different at p  0.05.
**Hydrophobicity of the coatings decreased from HL1 to HL3. SC of 2-HL1 coating was double that of HL1 coating.

Table 6. Effect of LBG-based coatings on quality of Burlat cherry juice stored 6 days at 1 °C followed by 1 day at
20 °C.
TA SS MI Ethanol Acetaldehyde
Treatment* (g malic/100 mL) °Brix (SS/TA) (mg/100 mL) (mg/100 mL)

HL1 0.58 a 18.3 a 31.7 b 3.0 a 1.18 a


HL2 0.57 a 18.9 s 32.9 b 5.5 ab 1.41 a
HL3 0.63 ab 19.9 ns 31.8 b 8.0 b 1.48 a
2-HL1 0.73 c 18.8 ns 25.7 a 6.5 b 1.28 a
Uncoated 0.68 bc 20.2 ns 29.6 ab 6.3 b 1.81 a

*Hydrophobicity of the coatings decreased from HL1 to HL3. Solids content of 2-HL1 coating was double that of HL1 coating. At harvest: 0.660 g malic
acid/100 mL; 16.6 °Brix; MI  25.2; EC  0.5 mg/100 mL; AC  1.15 mg/100 mL. Means within a column followed by different letters are significantly different at
p  0.05.

lightness and chroma loss. Remón (2001) reported a angle values, however there was no difference but at
decrease in lightness during storage of Burlat cherries, ambient temperature no differences were detected in
but chroma and hue angle showed oscillation without hue angle between coated and uncoated samples.
exhibiting a clear trend. In other red fruit, such as Given that changes in all three parameters were
‘Selva’ strawberries, decreases in the three chromatic detected, without exhibiting a similar trend in each
indexes (luminosity, hue angle and chroma) were treatment and showing fluctuations during storage, we
detected during storage (Holcroft and Kader, 1999). decided to study the Hunter total colour difference
In our work, coatings did not slow down the light- (E) compared to the initial colour before storage
ness loss during storage (Table 5). Coated cherries with (Table 5). E increased during storage (p  0.05).
the most hydrophilic coating (HL3) showed the lowest HL3-coated cherries showed the highest E values
L value after 6 days of storage at 1 °C plus 1 day at after the two storage periods, which indicated that this
20 °C, whereas the most hydrophobic coatings (HL1 coating was not effective in maintaining the colour of
and 2-HL1) reached the highest lightness value. After the cherries. On the other hand, the lower E values
11 days of storage at 1 °C plus 1 day at 20 °C, all the measured in HL1 and HL2 treatments after the first
coated cherries had lower lightness than the control, storage period indicated a delay in ripening. After 11
except for cherries coated with 2-HL1 which showed L days of storage at 1 °C plus 1 day at 20 °C, none of the
similar to the control. coatings were able to decrease colour difference. The
After the first storage time, HL1 and HL2-coated most hydrophilic coating induced the highest colour
cherries maintained higher chroma values than the difference. From these results, the E might be a
other treatments. Whereas, HL3, 2-HL1 and uncoated useful parameter in order to study postharvest colour
cherries had a dramatic decrease in chroma values evolution of cherries; but further studies are needed.
compared to values registered at harvest. However, Table 6 shows SSC, TA and MI of coated and
hue angle of control samples did not change from that uncoated samples after 6 days of storage at 1 °C plus 1
at harvest, but decreased for coated samples. Yaman day at 20 °C. Juice quality was not determined after 11
and Bayindirli (2002) reported that the Semperfresh days of storage at 1 °C plus 1 day at 20 °C due to the
coating was effective, maintaining skin colour of cher- high deterioration index (DI) observed in the fruit.
ries during cold storage, increasing lightness and hue Organic acids are substrates for the enzymatic
422 C. ROJAS-ARGUDO ET AL.
Table 7. Effect of LBG-based coatings on DI of Burlat cherries.
DI at storage

Treatment* 6 days at 1 °C  1 day at 20 °C 11 days at 1 °C  1 day at 20 °C

HL1 1.36 a 1.58 a


HL2 2.04 b 1.80 a,b
HL3 2.18 b 1.98 b,c
2-HL1 1.91 b 1.60 a
Uncoated 1.96 b 2.23 c

*Hydrophobicity of the coatings decreased from HL1 to HL3. SC of 2-HL1 coating doubled those of HL1 coating. DI range: 0–3. DI includes pathological and
physiological disorders. Means within each storage time with different letters are significantly different at p  0.05.

reactions of respiration which cause acidity loss during tricarboxylic acid cycle enzymes, succinate dehydro-
postharvest storage of fruits. However, after 6 days of genase activity (Mattheis et al., 1997). These authors
storage at 1 °C plus 1 day at 20 °C, the acidity of detected increases in ethanol accumulation of ‘Bing’
uncoated samples did not decrease compared to the cherries when CO2 exposure increased, but this accu-
acidity at harvest. Only samples coated with HL1 and mulation began after prolonged storage. Rojas and del
HL2 coatings had lower TA than the control. Probably, Río (2001) detected slight increases in ethanol content
the higher weight loss detected in uncoated cherries (EC) of coated ‘Burlat’ cherries using LBG–shellac
(Table 3) might have masked the acidity loss in this edible coatings and a coating containing glycerol esters
treatment. SSC increased in all samples compared to and dextrin derivatives, but did not detect an increase
SSC at harvest; however, no differences were found in EC using other LBG–composite film coatings. The
between treatments. Among coated treatments, 2-HL1 EC obtained was not sufficient to induce off-flavours in
coating (the highest SC) maintained the higher acidity cherries coated with glycerol esters and dextrin deriva-
and, therefore, had the lower MI, which indicated that tives, but cherries coated with LBG–shellac coating
SC affected acidity levels of the samples. The high were given the lowest scores by the sensory panel.
acidity level of 2-HL1 treatment contrasted with the fact
that this treatment had lower weight loss (Table 3) and Deterioration Index (DI)
then the organic acids were less concentrated. Yaman
and Bayindirli (2002) found that Semperfresh coating Cold storage, between 0 and 1 °C, is necessary for
was effective in maintaining titratable acidity and skin highly perishable fruits, such as cherries, to decrease
colour of cherries during storage; however, SSC and weight loss. However, low temperatures during storage
sugar content were not affected by coating applications. can induce cherry surface pitting (Facteau and Rowe,
1979). Some studies have shown that coating applica-
Flavour tion can alleviate chilling injury in chill-sensitive fruits,
such as mandarins (Martínez-Jávega et al., 1989; Pérez-
Flavour was not affected by coating application Gago et al., 2002) and that effect could be attributed to
after 1 week of storage and off-flavours were not a reduction in moisture loss and a modification of the
detected (results not shown). After 1 week of storage, internal atmosphere (Wang, 2000). Cherry surface
scores in all treatments ranged from 5.5 to 6.5 and pitting has also been attributed to mechanical injury
were, therefore, inside the acceptability ranking. Rojas during fruit handling (Porrit et al., 1971).
and del Río (2001) detected a decrease in flavour of Mould growth (rated as 3) and physiological disor-
shellac based-coated cherries after 1 week of storage. ders, such as surface pitting and bruising (rated from 1
to 3 according to size of the affected area) were con-
Ethanol and Acetaldehyde Contents sidered in the deterioration index (DI, Table 7). After
6 days of storage at 1 °C plus 1 day at 20 °C, DI was
Off-flavours can develop in fruit exposed to O2 high and ranged between 1 and 2. Uncoated samples
and/or CO2 as a result of anaerobic respiration and for- were evaluated as 2, which can be considered unac-
mation of ethanol and acetaldehyde (Kader, 1986). In ceptable from a commercial point of view. Among the
our work, edible coatings did not increase ethanol and coatings, only the HL1 coating was able to reduce the
acetaldehyde contents, despite the presence of an arti- DI of coated cherries. This treatment corresponded to
ficial barrier to gas diffusion around the fruit which the coating with higher hydrophobic content and
may result in reduced O2 and increased CO2 concentra- applied at low SC. After 11 days of storage at 1 °C plus
tion (Table 6). 1 day at 20 °C, the DI of uncoated samples significantly
Fermentative metabolism in cherries exposed to increased. However, the DI of some coated samples
high CO2 concentrations may occur due to inhibition of were either maintained, such as samples coated with
Postharvest Quality of Coated Cherries cv. ‘Burlat’ 423
HL2 and HL3 coatings, or reduced, such as samples Edible Coatings and Films to Improve Food Quality.
coated with 2-HL1. These results could be attributed to Lancaster, Penn.: Technomic Publishing Co., pp. 25–64.
biological variability. Nevertheless, values of DI close Baldwin E.A. (1999). Surface treatments and edible coat-
to or above 2 should be considered unacceptable from ings in food preservation. In: Shafiur Rahman M. (ed.).
the commercial point of view, which makes the HL1 Handbook of Food Preservation. New York: Marcel
coating the most appropriate treatment from the ones Dekker Inc., pp. 577–609.
tested to reduce physiological disorders. Beaudry R.M. (1999). Effect of O2 and CO2 partial pres-
sure on selected phenomena affecting fruit and veg-
etable quality. Postharvest Biology and Technology 15:
293–303.
CONCLUSION Chen P.M., Mellenthin W.M., Kelly S.B. and Facteau T.J.
(1981). Effects of low oxygen and temperature on
Coating composition affects cherry postharvest quality retention of ‘Bing’ cherries during prolonged
quality. Hydrophobic coatings improved cherry storage. Journal of the American Society for Horticul-
quality. Major benefits by coating application were tural Science 106: 533–535.
found in weight, firmness and DI. Colour was not Chen S. and Nussinovitch A. (2000). Galactomannans in
enhanced by coating application. As the hydrophilic disturbances of structured wax-hydrocolloid-based
character of the coating increased, weight and firmness coatings of citrus fruit (easy-peelers). Food Hydrocol-
loss was increased. Increasing SC did not improve loids 14: 561–568.
cherry quality in general. Colour, acidity and MI were Chen S. and Nussinovitch A. (2001). Permeability and
retained slightly with the higher SC coating, but the roughness determination of wax-hydrocolloid coatings,
high DI of this coating advises against its future appli- and their limitations in determining citrus fruit overall
cation. quality. Food Hydrocolloids 15: 127–137.
Davis P.L. and Chace W.C. (1969). Determination of
alcohol in citrus juice by gas chromatographic analysis
ACKNOWLEDGEMENTS of head space. HortScience 4: 117–119.
Drake S.R. (1991). Cherries: Preservation and quality
This work was funded by the INIA project No. standards. In: Eskin N.A. (ed.), Quality and Preserva-
tion of Fruits. Boca Raton, Fl.: CRC Inc., pp. 169–180.
SC98–056. The authors also thank Ceamsa for supply-
ing free samples of LBG. Drake S.R., Kupferman E.M. and Fellman J.K. (1988).
‘Bing’ sweet cherry (Prunus avium L.) quality as influ-
enced by wax coatings and storage temperature.
Journal of Food Science 53: 124–126, 156.
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