Republic of the Philippines

Department of Education
Region 1
Province of Ilocos Sur
Narvacan National Central High School
NarvacanIlocos Sur

AMPALAYA (Momordica charantia)and

MALUNGGAY (Moringa oleifera)

LEAVES EXTRACT AS OINTMENT

Micah Ellah Tejada

Shama Joy Cabico

Kimberly Ann Narce

Rona Cabrales

John Paulo Ramirez

Mark Aeron Yabut

Reseachers

Mrs. Lubeth Cabatu

Research II Adviser
 CHAPTER I

INTRODUCTION

1.1. Background of the Study

For centuries, people have used the plants that grew around them to treat wounds and help

speed healing. Nearly everyone is using commercialized ointments which contains chemical that

can have side effects and can further have infections. The researchers have decided why not

make alternative ointment since there are variety of other herbs traditionally used to heal minor

wounds, scrapes and burns.

Malunggay (Moringa Oleifera) contains lunasin, an antibiotic-like protection for the cells

which also has antifungal properties. Extracts of malunggay from leaves have been known for

decades to address the following: immune system detoxification; management of blood pressure,

cholesterol, blood sugar and speeds up healing of wounds. This plant is overflowing with

vitamins A, B, C, D, E and minerals including potassium, calcium, iron, selenium and

magnesium. Moringa leaves are completely safe to human consumption. It does not contain any

toxic elements or side-effects. It is packed with flavonoids- antioxidants necessary for fighting

against free radicals that are causing all sorts of problems in the human body. Also it is very

convenient since it grows very widely in tropical and subtropical area specifically in the

Philippines.

The ampalaya plant (momordica charantia), is a tropical plant found in Africa, Asia, South

America and the Caribbean. The historic crop is well known for its medicinal value, used as a

treatment for diseases of the skin and body and consumed for general health purposes. Ampalaya

has a long history in Ayurvedic and traditional Philippine medicine. These ancient systems of
medicine have consumed the bitter gourd and other parts of the plant for centuries, attributing its

many medicinal benefits to a multitude of healthy properties. Research and studies worldwide

proved the benefits of bitter melon on the body’s health. This vegetable was also considered by

Department of Health (DOH) as an alternative in treating certain diseases including skin disease,

liver problems, HIV, diabetes, and other ailments. It can relieve headaches, good for rheumatism

and gout, treat hemorrhoids, intestinal worms, and sure several types of cancer. It disinfect burns

and heals a wound, boost the immune system, fight infections, reduce blood pressure, and good

for the liver. The antimicrobial activity is beneficial for cleaning and healing wounds. So, the

researchers decided to combine these herbal plants to produce ointment because of its health

benefits.

1.2. Statement of the Problem

This study aimed to evaluate the produced ointment in curing wounds out of Ampalaya and

Malunggay leaves extract. Specifically, it seeks to answer the question:

1. Is it acceptable to produce an ointment in curing wounds out of Ampalaya and

Malunggay leaves extract in terms of:

a. Effectiveness, and

b. Odor

1.3 Significance of the Study

This study provides people with additional knowledge on the possible uses of Ampalaya

and Malunggay leaves like in the production of ointment in treating wounds. The study aims to

create a product out of Ampalaya and Malunggay leaves that can help in treating wounds. The
researcher brings out the present relevance of our human resources; talents, abilities and

potentiality to those who are in doubt; as the commercial one. In this study many people will be

benefited especially to those who can't afford expensive medicines in curing wounds; and this

study can also be a big help to those people who has a lot of ampalaya and malunggay plant in

their yards. And we must know how to use our nature very well because it is only for our own

good. Thus, enables them to save money.

1.4 Scope and Delimitations

This study will only focus on the production of ointment in curing wounds out Ampalaya

and Malunggay leaves and its effectiveness. This study will be conducted in one of the

researcher’s residence in Quinarayan, Narvacan, Ilocos Sur. In testing the product, people who

have wounds will be the respodents of the researcher’s. This study does not cover the other parts

of the plant of Ampalaya and Malunggay leaves.

1.5. Review of Related Literature

According to Organic facts, Malunggay (Moringa Oleifera) possesses antibacterial and

antimicrobial properties and is effective against the growth of disease-causing microbes.

Scientific research has proven that moringa extracts exert a wide spectrum of protective activity

against food-borne micro-organisms such as Salmonella, Rhizopus species, E. Coli, Enterobacter

aerogenus, Pseudomonas aeruginosa and Staphylococcus aereus, This defensive activity makes

moringa extracts perfect for sanitation and preservation purposes. Also, aqueous leaves extract of

moringa oleifera possess significant wound healing properties. Research studies have provided

supporting evidence regarding the healing effects of moringa in the treatment of wounds with

respect to skin-breaking strength, wound closure rate and reduction in the scar area.
 According to Scientific Journal, Malunggay (Moringa oliefera), a topical plant of varied

uses is often utilized in folk medicine to treat open wounds suggesting its antibiotic property.

This study investigated the antibiotic activity of malunggay against the pus-forming bacteria,

Staphylococcus aureus that are the common cause of wound infections in the community.

Bacteria were cultured from a swab of a 2-day old purulent wound, pure cultures were prepared,

and their identity verified by gram staining, culture appearance and differential tests results (e.g.,

catalase, blood agar hemolysis) with the assistance of a licensed bacteriology technologist. Juice

from freshly picked malunggay leaves was extracted with the use of a commercial juicer, filter-

sterilized and tested for antibiotic activity based on the Kirby Bauer Method. Zones of inhibition

on a lawn of the pure culture of S. aureus produced by filter discs presoaked in varied

concentrations (0%, 50%, 75% and 100%) of the malunggay extract were compared to the zones

of inhibition on the same culture plate produced by discs pre-soaked in a preparation of penicillin

G). Results showed significant increases in the diameter of the zones of inhibition produced with

increasing concentrations of the malunggay leaves extract suggesting that fresh extract of

malunggay leaves has antibiotic property that can be effective in the control of community-

acquired Staphylococcus infections. Further confirmation of its efficacy effectiveness against

drug resistant bacteria is recommended.

According to Ncbi, Momordica charantia (MC; bitter gourd) is a traditional herbal

commonly used for its antidiabetic, antioxidant, contraceptive and antibacterial properties. In the

current study, the authors aim to observe the topical effect of MC cream on the wound-healing

process in rabbits. Moreover, they compare the healing potential with conventional creams used

therapeutically. Towards this aim, 28 New Zealand rabbits were divided into four groups and

excision wounds (7 cm²) were made on their backs. Open wound dressing was carried out daily
for 28 days among the experimental groups with the application of dekspanthenol (Bepanthen®;

BP group, n = 7), nitrofurazon (Furacin®; FR group, n = 7) and olive oil extract of MC (MC

group, n = 7). No application was made to the control group. At the end of day 28, areas of the

skin with initial wound area were en bloc dissected and prepared for histopathological and

stereological analysis. Inflammatory cells were abundant in the control group and cream

application led to a decrease in the number of these cells, especially in the MC group. The

highest number of fibroblasts was detected in the MC group. Furthermore, the MC group

displayed the highest fractions of epidermis to papillary dermis, fibroblasts to reticular dermis

and collagen fibres to reticular dermis. The MC group also presented a high density of blood

vessels, moderate density of collagen fibres and mature fibroblasts. The BP group showed better

epithelialisation compared with the FR group, but the latter provided more effective

reorganisation of the dermis. Different cream supplements caused healthy and fast wound

healing according to untreated controls and the results show that administration of the MC

extract improves and accelerates the process of wound healing in rabbits in comparison with the

BP and FR extracts.
 CHAPTER II

MATERIALS AND METHODS

2.1. Research Design

This experimental-type of quantitative research deals with the usage of Ampalaya and

Malunggay leaves extracts for the production of ointment in curing wounds. The purpose was

to study the Ampalaya aang Malunggay leaves extracts as an alternative of ointment to help

in curing people who have wounds.

2.2. Statistical Treatment of Data

A. Materials and Equipments

The following ingredients will be used to make ointment out of Ampalaya and

Malunggay leaves: Extract of ampalaya and malunggay leaves are the main ingredient in making

ointment. These are the other materials and equipments needed in making ointment: a boiler,

strainer or clean cloth, oil, petroleum jelly and a container where you put the finished product.

B. Procedure

In a bowl over a pan of boiling water; melt the petroleum jelly. Then add the extract of

ampalaya and malunggay leaves, heat gently for about two hours. Pour the mixture into a jelly

bag or a piece of clean cloth that you have fitted around the rim of a jug and secured with a

rubber band or string. Whilst wearing rubber gloves (the mixture will still be very hot to handle)

squeeze the mixture through the clean cloth or jelly bag. Transfer the mixture into the container

while still warm. To avoid condensation allow the ointment to cool completely before securing

with a lid. Finished colour will depend on the herbs you choose to use.
 2.3 Flow Chart

This section presents the flow chart design of the study.

Gathering the Collecting and preparing Extract the gathered

Ampalaya and the other materials ampalaya and
Malunggay leaves needed in the study malunggay leavs

Testing Boil a water then melt the

Gathering Put the finished product
the petroleum jelly and add
of data. into the container.
product. the extracted leaves of
ampalaya and malunggay
then stir it.
 BIBLIOGRAPHY

Websites

https://malunggayointment.wordpress.com/

https://malunggayointment.wordpress.com/background-of-the-study/

http://pharmacyinformatics2014-csab.blogspot.com/2014/07/ampalaya.html

http://www.medicalhealthguide.com/articles/ampalaya.htm

https://ampalayaherb.wordpress.com/

http://herbs.lovetoknow.com/Ampalaya_Properties

https://www.scribd.com/document/356735299/MALUNGGAY-RRL

https://www.organicfacts.net/health-benefits/vegetable/moringa.html

https://www.ncbi.nlm.nih.gov/pubmed/22812507

http://asianscientificjournals.com/new/publication/index.php/ljher/article/view/671