Practical: Study of Karyotypes (Giant Polytene Chromosomes) in Drosophila Melanogaster (Small Fruit Fly) | Dr.

Javed Siddiqui

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Practical Model Paper

Practical: Study of Karyotypes (Giant Polytene
Practical: Study to demo… Chromosomes) in Drosophila Melanogaster (Small Fruit
Fly)
Practical: Study to demo…
STUDY OF KARYOTYPES (GIANT POLYTENE
CHROMOSOMES) IN
DROSOPHILA
MELANOGASTER (SMALL FRUIT FLY)
Practical: Study of early …

MATERIAL:      1. Drosophila Larva             6. Glass slides              11. 

Practical: Study of Stag…
Practical: Study of Cytoc…
Petri
Practical: Study o… 2 dish
Light Microscope   14.
Practical: Study of a Ge…
Multiple Alleles study in …
Plastic dropper
     2. Saline solution 0.7%         7. Tweezer                   12. 
Cover slips     
     3. Aceto-orcein stain             8. Filter papers            13. 
     4. Dissecting needles             9.
 Distilled Water          
     5. Dissecting Microscope     10. Acetic acid             15.  Nail
polish

Practical No. 3 for Zoolo… TECHNIQUE:  

       1.     
Wash hands and put on gloves.
Practical No. 2 for Zoolo…
       2.     
Remove a large active larva from the Drosophila culture bottle using
tweezers.
Practical No. 1 for Zoolo…
       3.     
Dissect the larva to pinch the head just below the mouthparts using
dissecting needles.
What can we do for coll…
       4.     
Pull out the bead-like salivary glands enclosed in fat and remove the
fat.
       5.     
Put the salivary glands to a very clean slide
       6.     
Add few drops of aceto-orcein stain on the salivary glands for 4
minutes
       7.     
Put the cover slip over the stained salivary glands
       8.     
Gently warm the slide and fold the filter paper over the slide.
       9.     
Apply the moderate pressure on the cover slip with your thumb.
     10.     Tap the slide with rubber end of a pencil so that the chromosomes may
be separated.
     11.      Make a permanent slide by applying nail polish across the cover slip.
     12.     First see the giant chromosomes under low power (10X) and then high
power (40X or 100X)                microscope.
     13.     You will observe four pairs of chromosomes having light and dark bands.

http://mjigilani.blogspot.com/2015/01/practical-study-of-karyotypes-giant.html?view=sidebar[8/6/2019 7:53:06 AM]

Practical: Study of Karyotypes (Giant Polytene Chromosomes) in Drosophila Melanogaster (Small Fruit Fly) | Dr. Javed Siddiqui

Figure
6a.  Salivary glands in the head region of Drosophila’s active large larva

Figure 6 b. Karyotype in the

salivary glands of Drosophila larva
Posted 1st February 2015 by Dr. Javed Siddiqui
Labels: Drosophila, Giant Chromosome, Karyotypes, Practical, Salivary glands, Small
Fruit Fly

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goutamchintala 27 November 2016 at 22:43

thank you sir

http://mjigilani.blogspot.com/2015/01/practical-study-of-karyotypes-giant.html?view=sidebar[8/6/2019 7:53:06 AM]

Practical: Study of Karyotypes (Giant Polytene Chromosomes) in Drosophila Melanogaster (Small Fruit Fly) | Dr. Javed Siddiqui

Reply

Unknown 24 January 2019 at 03:30

Good job sir but there is need to explain how can we dissect larva to take silvery
glands out.
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