Biochemistry for Medical Laboratory Science

Millon’s Test

MD2Y1 - 5

Group 6:
Bellen, Justin
Lopez, Jose Miguel
Murillo, Darcy
Santos, Apple Mae
Santos, Jackie
Tolosa, Mhike
Uichangco, Kraze Ann
Vidanes, Beatrix
Vijandre, Jason

Date performed: August 7, 2019

Score:
Introduction:
Millon’s test is specific to phenol containing structures (tyrosine is the only common
phenolic amino acid). Millon’s reagent is concentrated HNO3, in which mercury is dissolved
that causes it to form a red precipitate or solution, which is considered as positive. A yellow
precipitate of HgO is NOT a positive reaction but usually indicates that the solution is too
alkaline.
Materials:
 Test tube
 Beaker
 Hot plate
 Wire gauze
 Test tube clamp
 Water
 Albumin
 Tyrosine
 Phenylalanine
 Phenol
 Sodium Nitrite
Procedures:
1. Place 1 mL of albumin, tyrosine, phenylalanine, and phenol in separately labeled test
tubes.
2. Add 4 drops of Millon’s reagent into each test tubes.
3. Heat in boiling water bath for 10 minutes then cool by running into tap water.
4. Add 4 drops of Sodium Nitrite (NaNO2) and warm gently. Observe.

Results and Conclusion:

Sample Result
Albumin Formed a slightly red colored solution
Tyrosine Formed a red colored solution
Phenylalanine Did not react, still a clear white solution
Phenol Formed a red colored solution

This concludes that phenylalanine does not contain a phenol group that is why it did not
react while albumin, tyrosine, and phenol have a phenol group that is why it reacted and formed
a red colored solution.
 1. Phenol, Phenylalanine, Tyrosine, and Albumin solution after heating.

2. Phenol, Phenylalanine, Tyrosine, and Albumin solution after 4 drops of NaNO2.

References: http://www.chem.boun.edu.tr/wp-content/uploads/2014/04/Chem-415-Experiment-2.pdf