67th Conference on

Mass Spectrometry and Allied Topics

Sponsored by
June 2 - 6, 2019
American Society for Atlanta, Georgia
Mass Spectrometry

SHORT COURSES June 1 - 2

Susan Richardson
Vice President for Programs

Important Dates
January 15
Deadline to apply for
The conference and short courses will be held in the Georgia World Congress Center. Corporate
undergraduate or graduate
hospitality suites will be at the Omni CNN Center Hotel.
student conference
travel awards
The Georgia World Congress Center is surrounded by a range of hotels and restaurants in the heart of
February 1 downtown Atlanta.
Deadline for abstract Corporate Membership and Exhibits. The participation of ASMS Corporate Members is an integral part of
submission the conference. A Corporate Member exhibit booth or hospitality suite provides access to over 6,000 scientists
April 30 attending one of the most dynamic conferences in the world. Information is online at www.asms.org.
Deadline for advance Social Events.
conference registration and • Sunday: Welcome Reception in the poster/exhibit hall immediately follows the opening plenary session.
short course registration A buffet of heavy hors d’oeuvres, beer and soft beverages are free to all conference registrants.
• Monday - Thursday: Poster session coffee break and afternoon pick-me-up snacks in the poster/exhibit hall.
April 30 • Monday - Wednesday: Light snacks, beer and beverages bridge the transition from plenary session to
Deadline for hotel reservations workshops as you meet-up to discuss the science of the day.
at the conference rates • Thursday: Closing Event at the Georgia Aquarium. An incredible opportunity to visit one of the world’s
largest aquariums. A dinner buffet and full aquarium access is included. This event is a value of over $150.
Your cost is $40 per person. Purchase tickets online via conference registration. Tickets can be added on to
your existing conference registration at any time.
Travel and Accommodations.
Hartsfield-Jackson Atlanta International Airport (ATL) is one of the busiest in the world with daily non-
stop flights to hundreds of cities. Go to asms.org for details on airport public transportation (MARTA) and ride
share options.
Hotel reservations are managed online. Visit asms.org for hotels, rates and map.

www.asms.org

Go to www.asms.org to view a description of each course.
Short courses have limited enrollments. The deadline for registration is April 30. There is no on-site registration for short courses.
TWO-DAY SHORT COURSES
Saturday and Sunday, June 1 - 2, 2019
01 Bioinformatics for Protein Identification
Instructors: Nuno Bandeira (University of California San Diego),
Alexey Nesvizhskii (University of Michigan Health System), David Tabb
(Stellenbosch University)
02 Clinical Diagnostics: Innovation, Validation, Implementation and
Operation by Mass Spectrometry
Instructors: Matthew Crawford, Brian Rappold and Chris Shuford (LabCorp)
03 DMPK: Experimentation and Data Interpretation
Instructors: Mark Hayward (ITSP Solutions and Active Ingredient Tech-
nologies), Mike S. Lee (Milestone Development Services), Naidong Weng
(Janssen Research and Development), Mingshe Zhu (Bristol-Myers Squibb)
04 Glycans and Glycoproteins in Mass Spectrometry
Instructors: Jon Amster (University of Georgia), Carlito Lebrilla (University
of California, Davis), Ron Orlando (University of Georgia), Joe Zaia (Boston
University)
05 High Resolution Mass Spectrometry for Qualitative and
Quantitative Analysis: An Introduction
Instructors: Ragu Ramanathan, Matthew Blatnik, Cong Wei (Pfizer)
06 Understanding, Optimizing and Applying LC/MS/MS Techniques
using Electrospray, APCI and APPI to Develop Successful Methods
Instructor: Robert D. Voyksner (LCMS Limited)
Short Courses
07 LC-MS: Advanced Techniques and Applications
Instructor: Jack Henion (Advion, Inc.)
08 LC-MS: Practical Maintenance and Troubleshooting
Instructors: Susan Abbatiello (Northeastern University), Thomas Blau (Ion
Technology Support, Inc), Will Thompson (Duke University)
09 Native Mass Spectrometry
Instructors: Vicki Wysocki (The Ohio State University), Sophie Harvey
(The Ohio State University), David Russell (Texas A&M University), Arthur
Laganowski (Texas A&M University)
10 Peptides and Proteins in Mass Spectrometry
Instructors: Arthur Moseley (Duke University), Douglas Sheeley (NIH), Eric
Soderblom (Duke University)
11 Protein Structural Analysis by Mass Spectrometry: Hydrogen
Exchange and Covalent Labeling
Instructors: Mark Chance (Case Western Reserve), Roxana Iacob
(Northeastern University), Janna Kiselar (Case Western Reserve), Thomas
Wales (Northeastern University)
12 Protein Therapeutics: Practical Characterization and
Quantitation by Mass Spectrometry
Instructors: Guodong Chen and Li Tao (Bristol-Myers Squibb)
13 Quantitative Mass Spectrometry
Instructors: Dwight E. Matthews (University of Vermont) and Robert Bethem
(Consultant)
14 Case Studies in Quantitative Proteomics
Instructors: Meena Choi (Northeastern University), Jake Jaffe (The Broad
Institute), Brendan MacLean (University of Washington), Olga Vitek
(Northeastern University)
15 Untargeted Metabolomics: From Basic Methods to Advanced
Workflows and Isotope Labeling
Instructor: Gary Patti (Washington University School of Medicine)
ONE-DAY SHORT COURSES
Sunday June 2, 2019
17 Introduction to GLP Regulations and Bioanalytical Method
Validation by LC-MS/MS (Sunday only)
Instructor: Perry Wang (LC-MS Technical Expert)
18 Ion Mobility Mass Spectrometry: An Introduction to
Instrumentation, Applications, and Data Analysis (Sunday only)
Instructors: Erin Baker (North Carolina State University), Brian H. Clowers
(Washington State University), John A. McLean (Vanderbilt University)
19 Advanced Metabolomics: Bioinformatics, Activity &
Systems Biology
Instructors: Gary Siuzdak, H. Paul Benton, Xavi Dominguez and Erica
Majumder (The Scripps Research Institute)

2019 PROGRAM OVERVIEW

SATURDAY JUNE 1 TUESDAY JUNE 4
9:00 am - 5:00 pm Short Courses 8:30 - 10:30 am Oral Sessions
2:00 - 5:00 pm Conference Registration • TOA Informatics: Innovations
• TOB Fundamentals: Photoionization and Photodissociation
SUNDAY JUNE 2 • TOC Native MS in Structural Biology
9:00 am - 5:00 pm Short Courses • TOD Imaging: Pharmaceuticals, Metabolites, and Lipids
10:00 am - 8:00 pm Conference Registration • TOE Environmental: Emerging Contaminants (In Honor of Ron Hites)
5:00 - 6:30 pm Tutorial Lectures • TOF Protein-Ligand Interactions
Targeted Imaging Data Independent Acquisition • TOG MS in the QC Lab
Enrico Davoli Birgit Schilling • TOH Nucleic Acids and Oligonucleotides
(Mario Negri Institute) (Buck Institute) 10:30 am - 2:30 pm Poster Session, Exhibits, Lunch Break
2:30 - 4:30 pm Oral Sessions
Native Mass Spectrometry Lipidomics
• TOA Informatics: Data-Independent Acquisition
Michal Sharon Stephen Blanksby
• TOB GCxMS, GC/GC/MS, GC-MS/MS, and GC/HRMS
(Weizmann Institute) (Queensland University of Technology)
• TOC Top Down Protein Analysis
Gavin Reid
• TOD Drug Target Identification by MS
(University of Melbourne)
• TOE Food Safety & Chemistry: Innovations
6:45 - 7:45 pm Opening Session: Plenary Lecture • TOF Cancer Research
• TOG Instrumentation: Innovative Separations Approaches Coupled to MS
Mark Z. Jacobson (Stanford University) • TOH Energy, Petroleum, and Biofuels: Instrumentation and Applications
Transitioning the World Energy for All Purposes
to Stable Electricity Powered by 100% Wind, 4:45 - 5:30 pm Plenary Session: Biemann Medal
Water, and Sunlight 5:45 - 7:00 pm Workshops
After 8:00 pm Corporate Hospitality Suites, Omni CNN Center Hotel
7:45 - 9:30 pm Welcome Reception in the Poster/Exhibit Hall
WEDNESDAY JUNE 5
MONDAY JUNE 3 8:30 - 10:30 am Oral Sessions
8:30 - 10:30 am Oral Sessions • WOA Metabolomics: New Technologies and Applications
• MOA Cannabis Testing • WOB Carbohydrates
• MOB Glycopeptides and Glycoproteins • WOC Fundamentals for Everyone: Peptides and Proteins
• MOC Membrane Protein MS • WOD Microdosing and Microsampling: Analytical Challenges
• MOD Imaging: Instrumentation & Method Development • WOE Environmental: Innovative Approaches and Instrumentation
• MOE Lipidomics: Targeted and Untargeted • WOF Ion Mobility: New Developments & Applications
• MOF Fundamentals: Ion Mobility and MS (In Memory of Al Yergey) • WOG Fundamentals for Everyone: Structural Elucidation
• MOG Instrumentation: Portable and Transportable Mass Spectrometers • WOH Synthetic Polymers
• MOH Biomarkers: Qualitative Analysis
10:30 am - 2:30 pm Poster Session, Exhibits, Lunch Break
10:30 am - 2:30 pm Poster Session, Exhibits, Lunch Break 2:30 - 4:30 pm Oral Sessions
2:30 - 4:30 pm Oral Sessions • WOA Metabolomics: Untargeted Profiling
• MOA Informatics: Multiomics Integration and Applications • WOB Hydrogen-Deuterium Exchange MS: Innovations
• MOB Homeland Security: Chemical/Biological Defense • WOC Forensics: Innovations and Applications
• MOC Food Safety & Chemistry: Foodomics, Allergens, Bacteria, Foods, and Supplements • WOD Endogenous Protein Biomarkers in Drug Discovery and Development: Quantitative
• MOD Therapeutic Proteins, Antibodies, and Antibody/Drug Conjugates Analysis
• MOE Lipidomics: New MS Technologies and Applications • WOE Clinical Analysis: MS in the Operating Room
• MOF Biomarkers: Quantitative Analysis • WOF Ion Mobility: Small Molecules, Pharmaceuticals, and DMPK
• MOG Instrumentation: New Developments in Ionization and Sampling • WOG Instrumentation: Ambient Ionization & Applications
• MOH Art, Archaeology, and Paleontology • WOH Fundamentals: DDA and DIA LC-MS
4:45 - 5:30 pm Plenary Session: Award for a Distinguished Contribution in Mass Spectrometry 4:45 - 5:30 pm ASMS Meeting
5:45 - 7:00 pm Workshops 5:45 - 7:00 pm Workshops
After 8:00 pm Corporate Hospitality Suites, Omni CNN Center Hotel After 8:00 pm Corporate Hospitality Suites, Omni CNN Center Hotel
 Program Overview
THURSDAY JUNE 6 • ThOE Plant “omics”
8:30 - 10:30 am Oral Sessions • ThOF Ion Mobility: Structure
• ThOA Informatics: Metabolomics • ThOG Instrumentation: Innovations in Mass Analyzers
• ThOB Fundamentals: Ion Spectroscopy • ThOH Fundamentals: Ion Activation and Dissociation
• ThOC Posttranslational Modifications: Qualitative and Quantitative
4:45 - 5:30 pm Closing Session: Plenary Lecture
• ThOD Drug Discovery and Development: Quantitative Analysis
• ThOE Supramolecular and Macromolecular Complexes Lilly D’Angelo (Global Food & Beverage Technology Associates)
• ThOF Clinical Analysis using MS Chemistry of Food and Soft Drinks
• ThOG Stable Isotope Labeling in MS: Applications

• ThOH Exposomics, Toxicology, and Human Health
10:30 am - 2:30 pm Poster Session, Exhibits, Lunch Break 6:30 - 9:30 pm Closing Event, Georgia Aquarium (advance ticket purchase required)
2:30 - 4:30 pm Oral Sessions
• ThOA Informatics: Peptide and Protein Identification, Proteomics
• ThOB Microorganisms and the Microbiome
• ThOC Quantitative Proteomics in Systems Biology
• ThOD Covalent Labeling and Chemical Crosslinking

ASMS CONFERENCE & SHORT COURSE REGISTRATION, Deadline: April 30, 2019
Register online: www.asms.org The deadline for advance registration is April 30. After April 30, fees increase by $50. Short Course registration closes on April 30.
Cancellations. To cover the cost of handling and processing, $40 is deducted from the total refund for cancellations. Conference registration cancellation must be received in writing on or before May 15, 2019. Short Course cancellation
must be received by May 15.
Name: I am an UNDERGRADUATE student.
Company/Institution:
Address:
Address:
Phone: Email:
Advance Conference Registration
 ASMS Member, $200*  ASMS Student Member, $90**  Non-member, $400
 Student Non-member, $160**  Emeritus Member, No Charge Enter registration amount $ ___________

Check ONE course for which you wish to register

ü TWO-DAY SHORT COURSES (Saturday and Sunday) ASMS member* Non-Member Student**
01 Bioinformatics for Protein Identification $400 $700 $150
02 Clinical Diagnostics by MS, Innovation, Validation, Implementation, and Operation by MS $400 $700 $150
03 DMPK: Experimentation and Data Interpretation, includes cost of textbook. $506 $806 $256
04 Glycans and Glycoproteins in Mass Spectrometry $400 $700 $150
05 High Resolution MS for Qualitative and Quantitative Analysis: An Introduction $400 $700 $150
06 Understanding, Optimizing and Applying LC/MS/MS Techniques using Electrospray, APCI and APPI to Develop Successful Methods $400 $700 $150
07 LC-MS: Advanced Techniques and Applications $400 $700 $150
08 LC-MS: Practical Maintenance and Troubleshooting $400 $700 $150
09 Native Mass Spectrometry $400 $700 $150
10 Peptides and Proteins in Mass Spectrometry $400 $700 $150
11 Protein Structural Analysis by Mass Spectrometry, includes cost of textbook $477 $777 $227
12 Protein Therapeutics: Practical Characterization and Quantitation $400 $700 $150
13 Quantitative Mass Spectrometry $400 $700 $150
14 Case Studies in Quantitative Proteomics $400 $700 $150
15 Untargeted Metabolomics: From Basic Methods to Advanced Workflows and Isotope Labeling $400 $700 $150

Check one or two courses for which you wish to register

ü ONE-DAY SHORT COURSES Member* Non-Member Student**
17 Intro to GLP Regulations and Bioanalytical Method Validation by LC-MS/MS (Sunday only) $300 $500 $75
18 Ion Mobility MS: Instrumentation, Applications, & Data Analysis (Sunday only) $300 $500 $75
19 Advanced Metabolomics: Bioinformatics, Activity & Systems Biology (Sunday only) $300 $500 $75

* To register as a member, ASMS dues for 2019 must have been processed by ASMS prior to conference registration.
** To qualify for the student rate, you must be enrolled full-time in a degree program up to and including the Ph.D. at the time of the conference Enter short course amount $ ___________
Spouse/Companion includes Welcome Reception on Sunday and name badge. Does not give access to scientific talks, posters or exhibits. Enter $10. Enter companion amount $ ___________
Closing Event Thursday, June 6. $40 per registrant. (Limit one ticket per registrant & one ticket per spouse/companion,
see below for spouse/companion closing event ticket) Closing Event amount $ ___________
Closing Event for Spouse/Companion Thursday, June 6. $40 per registered spouse/companion. Closing Event for spouse/companion amount $ ___________
Separate Spouse/Companion registration ($10) is required.
ENTER TOTAL AMOUNT $ ___________
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 Please charge the TOTAL AMOUNT to my Visa, MasterCard, or AMEX.

Visa, MasterCard, or Amex Number Security Code Expiration Date

Card Holder’s Name (please print) Card Holder’s Signature

Send this form with payment to: ASMS Conference, 2019 Galisteo Street, Building I-1, Santa Fe, NM 87505
Email to info@asms.org or Fax: (505) 989-1073 (Email or Fax registrations must include credit card information.)
 TWO-DAY SHORT COURSES • ATLANTA
Saturday and Sunday, June 1 and 2, 2019
Go to www.asms.org for more detailed descriptions, sample slides, and registration.
01 Bioinformatics for Protein Identification
Bioinformatics tools are routinely used to identify proteins from
“shotgun” LC-MS/MS data sets. These tools, however, are often
poorly understood by their users. This course seeks to familiarize
proteomics researchers with the inner workings of the software
that enables this field. The course will emphasize the key steps
in protein identification: peptide-spectrum matching, error rate
estimation, and protein assembly. It will build on these topics
by discussing advanced techniques for MS/MS identification
through spectral libraries and algorithms that leverage de novo
sequence inference. Live demonstrations of the Trans-Proteomic
Pipeline, ProteoSAFe, and BumberDash / IDPicker tools will
familiarize participants with the decisions that produce reliable
results from these systems. Participants will emerge from the
course with a solid understanding of the underlying algorithms
that enable protein identification as well as the software available
to accomplish this task.
Instructors: Nuno Bandeira (University of California San Diego),
Alexey Nesvizhskii (University of Michigan Health System), David
Tabb (Stellenbosch University)
02 Clinical Diagnostics: Innovation, Validation,
Implementation and Operation by Mass Spectrometry
This course will cover aspects of mass spectrometric analysis as
applied to clinical diagnostics. Rather than approach the use of
diagnostic mass spectrometry from the ground-up, the course
will focus on those components which are singular to the clinical
diagnostics industry. Attendees will be provided with details and
practical examples of laboratory operations in an environment
which is regulated by the FDA Office of In Vitro Diagnostics (FDA-
IVD), College of American Pathologists (CAP) and the Clinical
Laboratory Improvement Act (CLIA).
Instructors: Matthew Crawford, Brian Rappold and Chris Shuford
(LabCorp)
03 DMPK: Experimentation and Data Interpretation
Mass spectrometry has become the dominant tool throughout
the drug discovery / development continuum. This short course
will provide a thesis on mass spectrometry in drug metabolism,
pharmacokinetics (DMPK), and pharmacodynamics (biomarker)
in support of R&D and the registration process. The course will
use case studies to focus on the “why” and “how” knowledge
base with regard to the use of mass spectrometry to measure
small molecule drugs, biologics, and their conjugates in the
discovery and development phases. Contents will include an
introduction to the concepts / principles of DMPK, an overview of
drug discovery / development processes, regulatory submission
requirements, and common practices in DMPK studies. Current
mass spectrometry technologies applied in ADME screening
in lead optimization, drug quantification in PK studies, drug
metabolite identification in animals and humans, as well as
GLP bioanalysis quantification in clinical and toxicology studies
will be discussed along with updated industry practices for
experimental design, data interpretation, and data reporting.
Practice sessions will be given to reinforce data analysis
techniques learned in class.
www.asms.org
Instructors: Mark Hayward (ITSP Solutions and Active Ingredient
Technologies), Mike S. Lee (Milestone Development Services),
Naidong Weng (Janssen Research and Development), Mingshe
Zhu (Bristol-Myers Squibb)
04 Glycans and Glycoproteins in Mass Spectrometry
This course is designed for scientists who want to learn specific
techniques for the MS and MS/MS characterization of glycans
and glycoproteins. The course will address fundamental
aspects of glycobiology, sample preparation and handling,
mass spectrometry (hardware and software), and bioinformatic
tools for interpretation of results. Real-world examples of the
application of these techniques will include characterization
of intact glycoproteins, characterization of released glycans,
analysis of complex mixtures of glycoproteins and glycans. The
role of MS-based methods in interdisciplinary efforts to solve
these complex problems will also be addressed.
Instructors: Jon Amster (University of Georgia), Carlito Lebrilla
(University of California, Davis), Ron Orlando (University of
Georgia), Joe Zaia (Boston University)
05 High Resolution Mass Spectrometry for Qualitative and
Quantitative Analysis: An Introduction
This introductory course is designed to teach participants
the utility, advantages, and limitations of conducting LC-
MSn analysis using high resolution mass spectrometry. The
fundamental concepts of high resolution and accurate mass
measurement will be developed, as will techniques for mass
measurement and mass calibration. Current high resolution
instrument options including time-of- flight (TOF) and Fourier
transform mass spectrometry (FTMS) will be described and
compared. Mass analyzer options will be extended into MS/
MS platforms including hybrid instruments such as Q-TOF and
LTQ-FTMS. Qualitative and quantitative applications of high
resolution mass spectrometry will be discussed, especially for
small-molecule pharmaceutical compounds, biologics (antibody
drug conjugates, peptides, and proteins) and biomarkers.
These applications will include molecular formula and structure
analyses such as identification of metabolites, as well as effects
of high resolution on sensitivity and specificity in quantitation.
Instructors: Ragu Ramanathan, Matthew Blatnik, Cong Wei (Pfizer)
 TWO-DAY SHORT COURSES • ATLANTA
Saturday and Sunday, June 1 and 2, 2019
06 Understanding, Optimizing and Applying LC/MS/MS
Techniques using Electrospray, APCI and APPI to Develop
Successful Methods
This course is designed for the chromatographer / mass
spectrometrist who wants to be successful in developing
methods, optimizing methods and solving problems using LC/
MS. The course covers the atmospheric pressure ionization
(API) techniques of electrospray, pneumatically assisted
electrospray and atmospheric pressure chemical ionization
(APCI) and atmospheric pressure photo ionization (APPI) using
single quadrupole, triple quadrupole, time-of-flight and ion
trap mass analyzers. Discussions of sample preparation and
modes of chromatography will target method development
and optimization for the analysis of “real-world” samples by LC/
MS. The course highlights the following topics with respect to
optimization methods to achieve the best sensitivity, specificity
and sample throughput.
Instructor: Robert D. Voyksner (LCMS Limited)
07 LC-MS: Advanced Techniques and Applications
This two-day course presents a comprehensive overview of
technology and techniques of analytical mass spectrometry
and from that foundation extends into exciting, disruptive
recent developments. Course will include sample preparation;
advanced separation techniques; ionization techniques for MS;
mass Analyzers; imaging and profiling by MS; high resolution
MS; miniaturization in MS; and synergistic integration.
Instructor: Jack Henion (Advion, Inc.)
08 LC-MS: Practical Maintenance and Troubleshooting
With expanding use of LC-MS in laboratories not specifically
focused or trained in analytical chemistry, the analytical skills
needed to maintain high instrument performance and data
quality are less often part of the central curriculum or training of
instrument operators in these laboratories.  Indeed, high-end LC-
MS systems are now routinely found far outside of the traditional
analytical chemistry laboratory, including labs focused on
pharmaceutical, biochemical, biological, or environmental
applications. This is a natural progression as LC-MS technologies
become more mature and robust, however the increased use
of LC-MS has not removed the need for basic troubleshooting,
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instrument maintenance and repair skills, which are critical for
limiting downtime and providing cost savings to any laboratory.
Instructors: Susan Abbatiello (Northeastern University),
Thomas Blau (Ion Technology Support, Inc), Will Thompson
(Duke University)
09 Native Mass Spectrometry
Native mass spectrometry is a growing area in the mass
spectrometry community. Native MS is making significant
contributions to structural biology and because of its ease of
application and broad applicability, often leads and guides
an integrated approach that eventually uses other structural
biology tools. This course is designed to introduce the broad field
to new practitioners of native MS. Topics to be covered include
sample preparation for native MS; instrumentation used for
native MS - how does it differ from other MS; spray conditions to
keep soluble complexes native; spray conditions for membrane
protein complexes (micelles, liposomes, nanodiscs); activation
methods for native MS (CID, CIU, SID, UVPD, ETD/ECD/EID);
complementary methods (HDX, covalent labeling, crosslinking,
top-down MS); ion mobility for native MS; online separations
approaches coupled to native MS (IEX, SEC, CE); software tools
for native MS; (Unidec, Impact, Pulsar, EMnIM, DynamXL, XLinkX,
iFAMS); case studies for protein:protein, protein ligand, RNA:
protein, DNA:protein, and membrane protein complexes.
Instructors: Vicki Wysocki (The Ohio State University), Sophie
Harvey (The Ohio State University), David Russell (Texas A&M
University), Arthur Laganowski (Texas A&M University)
10 Peptides and Proteins in Mass Spectrometry
Over the past two decades, mass spectrometry has become the
key technology for the characterization of proteins. This course is
designed as an introduction for researchers needing to expand
their knowledge of the use of mass spectrometry-based methods
for the identification, characterization, and quantification of
peptides and proteins. Background material in basic protein
chemistry will be provided along with a review of mass analyzers,
acquisition types, and ionization sources used for protein and
peptide analysis. Real-world examples will be used to illustrate
protein sample preparation strategies, characterization of intact
proteins, characterization of post-translational modifications
(with emphasis on glycosylation and phosphorylation),
identification of proteins via database searching, direct
identification of proteins from gels and complex mixtures, and
quantitative differential protein expression studies. The role of
MS-based methods in interdisciplinary efforts to solve complex
biomedical problems will also be addressed. Additionally, there
will be tutorials on the use of open source proteomic software
tools for interpretation of proteomic datasets. Problem sets
will be used to emphasize practical aspects of comprehensive
protein characterization, including peptide sequencing and
protein identification by database searching.
Instructors: Arthur Moseley (Duke University), Douglas Sheeley
(NIH), Eric Soderblom (Duke University)
 TWO-DAY SHORT COURSES • ATLANTA
Saturday and Sunday, June 1 and 2, 2019
11 Protein Structural Analysis by Mass Spectrometry:
Hydrogen Exchange and Covalent Labeling
This course is designed for those who wish to understand protein
structure with mass spectrometry.
We will focus on covalent labeling for half of the course and on
hydrogen exchange for the second half. There will be a discussion
of the theory behind each labeling method as it relates to
proteins in solution, the general methodological steps one takes
to do these analyses, and a guide on how to process and interpret
the resulting data. There will be a discussion of the pitfalls one
can encounter during these experiments. Specific examples of
protein structure analysis will be provided in applications like
enzymology, protein-ligand interactions, protein dynamics, and
membrane proteins. Course participants will receive a copy of
a book from Wiley edited by the course instructors providing
detailed additional background on all the approaches.
Instructors: Mark Chance (Case Western Reserve), Roxana Iacob
(Northeastern University), Janna Kiselar (Case Western Reserve),
Thomas Wales (Northeastern University)
12 Protein Therapeutics: Practical Characterization and
Quantitation by Mass Spectrometry
This introductory course is designed for practicing analytical
scientists (new users, chromatographers, analytical chemists,
protein chemists, and laboratory managers) performing/
supporting recombinant protein characterization/analysis, in-
process testing, quality control, quality assurance, research and
development, and manufacturing. It covers the fundamental
principles and practical applications of mass spectrometry (MS)
for the characterization and quantitation of protein therapeutics
with a focus on monoclonal antibodies including antibody-
drug conjugates (ADCs). It emphasizes problem-solving skills
in the areas where MS is used most extensively, for example,
characterization of chemical modifications and post-translational
modifications of protein therapeutics. The interpretation of
protein therapeutics mass spectra will be illustrated with real
case examples. This course will focus on electrospray ionization
(ESI) and matrix-assisted laser desorption / ionization (MALDI)
techniques in the analysis of protein therapeutics, and will
survey various mass analyzer options for the characterization of
protein therapeutics. A thorough coverage of approaches toward
method development for both qualitative and quantitative
analysis of protein therapeutics will provide a good starting
point for understanding practical issues that are frequently
encountered during implementation and applications of MS on
protein therapeutics in the laboratory.
Instructors: Guodong Chen and Li Tao (Bristol-Myers Squibb)
13 Quantitative Mass Spectrometry
This introductory/intermediate level course explores the
principles of quantitative mass spectrometry as they apply
primarily to small molecule analyses. Topics that will be discussed
include: (a) MS instrumentation used for quantitative analyses;
(b) how a quantitative assay is set up including statistics for
quantitation, preparation and evaluation of standard curves
and analysis of variance; (c) LC/MS/MS method development
for target quantitative analysis of small molecules, including
www.asms.org
instrument and assay optimization procedures; (d) real world
approaches to assessing problems and improving method
ruggedness; (e) general guidelines for method validation in
a regulated environment; (f ) an introduction to quantitation
of endogenous compounds in biological matrices; and (g) an
introduction to quantitation in proteomics. Ample time will be
provided for general discussion of the topics presented and
other current topics in quantitative MS.
Instructors: Dwight E. Matthews (University of Vermont) and
Robert Bethem (Consultant)
14 Case Studies in Quantitative Proteomics
This course will explore practical aspects of setting up and
performing a quantitative proteomics experiment. The course
will focus on how to develop methods and perform the
analysis using the Skyline Software Ecosystem (http://skyline.
ms). Specifically, we will cover practical issues we have faced in
performing data collection and in the interpretation of data using
case studies from our own labs. At the end of the course each
participant will be familiar with introduction to quantitation of
proteins by mass spectrometry; strategies for peptide selection
as a proxy for a protein; using Skyline for method refinement and
data analysis; automated strategies for LC-MS system suitability
monitoring; visualization and assessment of multiplex peptide
LC-MS/MS data; sharing data using Panorama; assessing assay
figures of merit; different mass spectrometry data acquisition
strategies used in proteomics.
Instructors: Meena Choi (Northeastern University), Jake
Jaffe (The Broad Institute), Brendan MacLean (University of
Washington), Olga Vitek (Northeastern University)
15 Untargeted Metabolomics: From Basic Methods to
Advanced Workflows and Isotope Labeling
It has become relatively routine to acquire mass spectrometry-
based metabolomic data, either in one’s own laboratory or using
one of the many service facilities around the world. Despite this
progress, however, interpreting metabolomic results continues
to be a major challenge for many researchers that severely
limits potential applications of the technology. The overarching
objective of the two-day metabolomics short course is to guide
students to overcome this critical interpretation barrier.
Instructor: Gary Patti (Washington University-St Louis)
 ONE-DAY
ONE-DAY SHORT
SHORT COURSES
COURSES • ATLANTA
Sunday, June 2, 2019
Go to www.asms.org for more detailed descriptions, sample slides, and registration.
17 Introduction to GLP Regulations and Bioanalytical 19 Advanced Metabolomics: Bioinformatics,
Method Validation by LC-MS/MS Activity & Systems Biology
Sunday only Sunday only
“GUIDANCE FOR INDUSTRY/Bioanalytical Method Validation” The field of metabolism has had resurgence due to major
represents the Food and Drug Administration’s current thinking technological advances in mass spectrometry, which has
on this topic and was first published in May 2001. Since then, enabled new insights into the involvement of metabolites in
almost all regulated bioanalytical methods have been validated biological processes and enzymatic pathways. It is one of the
based on the guidance even though it does not create or fastest growing areas of science today and its primary analysis
confer any rights for or on any person and does not operate tool is mass spectrometry. The Advanced Metabolomics course
to bind FDA or the public. In September 2013, FDA published will complement the other ASMS Metabolomics short course
a draft guidance, which provides general recommendations for going into greater depth on key informatic topics that are critical
bioanalytical method validation using advanced technologies. to scientists trying to establish themselves in the field. Topics
The content of the original guidance issued in 2001 was revised to include metabolomics activity screening, assessing statistically
reflect advances in science and technology related to validating relevance, pathway analysis, and systems biology guided by
bioanalytical methods. This one-day short course will focus on metabolomics.
GLP regulations and the bioanalytical method validation for Instructors: Gary Siuzdak, H. Paul Benton, Xavi Dominguez and
drugs and metabolites in biological matrices using LC-MS/MS. Erica Majumder (The Scripps Research Institute)
It will help audiences to comply with FDA’s regulations for drug
discovery and development in the pharmaceutical industry
and CROs. The short course will also reflect the contents of the
updated guidance and recently published white papers with
regard to bioanalytical method validation using LC-MS/MS.
International harmonization of bioanalytical method validation
guidance will be discussed.
Instructor: Perry Wang (LC-MS Technical Expert)

18 Ion Mobility Mass Spectrometry: An Introduction to

Instrumentation, Applications, and Data Analysis
Sunday only
Realization of the value-added by combining ion mobility
spectrometry (IMS) and mass spectrometry (MS) has generated
significant interest in its use in range of fields including omics
pursuits, threat detection, and fundamental studies of gas-
phase ion chemistry. This course provides students with a basic
understanding of the most common instrumental configurations,
experimental sequence, best practices, and the theory underlying
the different types of ion mobility systems employed both in
academic and commercial settings. An overview of hyphenated
IMS-MS instrumentation with multiple practical applications and
experimental designs will be presented including comparative
discussions on advantages and disadvantages between different
stand-alone and hyphenated techniques. This course will provide
a comprehensive look at ion mobility spectrometry and its use
in modern analytical chemistry. While ion mobility systems are
largely compatible with all mass spectrometry systems each IM-
MS combination possesses a range of unique advantages and
applications. These instrumental considerations and specific
experimental sequences will be covered in this course. Graduates
of the course will have gained a detailed understanding of IMS
and an overview of its practical applications for both stand-alone
and MS-coupled operation.
Instructors: Erin Baker (North Carolina State University), Brian
H. Clowers (Washington State University), John A. McLean
(Vanderbilt University)

www.asms.org