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0.18
0.12
ABSORBANCIA (595nm)
0.1
0.08
0.06
0.04
0.02
0
0 0.1 0.2 0.3 0.4 0.5 0.6
-0.02
CC de Proteina (g/L)
Solucion Estandar Agua destilada C (g/l) Abs (540 nm)
500 0 2 1.018
400 100 1.6 0.823
300 200 1.2 0.617
200 300 0.8 0.44
100 400 0.4 0.173
50 450 0.2 0.062
0 500 0 0
1.2
1 y = 0.5232x - 0.0158
R² = 0.9968
0.8
ABSORBANCIA
0.6
0.4
0.2
0
0 0.5 1 1.5 2 2.5
-0.2
CONCENTRACION (G/L)
N° Solución agua glucosa ABS (505
estándar nm)
1 500 0 2 0.593
2 400 100 1.6 0.479
3 300 200 1.2 0.353
4 200 300 0.8 0.232
5 100 400 0.4 0.109
6 50 450 0.2 0.083
7 25 475 0.1 0.025
8 0 500 0 0
0.7
y = 0.2953x + 0.0017
0.6 R² = 0.9979
0.5
0.4
0.3
0.2
0.1
0
0 0.5 1 1.5 2 2.5
Esquema de la técnica experimental
N° MUESTRA
Solucion (A),mL Solucion (B),mL Solucion (C),mL Conc.sacarosa g/L
1 26 1 3 2.00
A1 2 23 1 6 4.00
B1 3 20 1 9 6.00
C1 4 18 1 11 7.33
A2 5 15 1 14 9.33
B2 6 12 1 17 11.33
C2 7 9 1 20 13.33
C3 8 6 1 23 15.33
A3 9 3 1 26 17.33
B3 10 0 1 29 19.33
𝐶1 𝑥𝑉1 = 𝐶2 𝑥𝑉2
A1 B1 C1 A2 B2 C2 C3 A3 B3
1 2 3 4 5 6 7 8 9 10
DNS min
0 - 0.4403670 4.3593941 0.3612385 2.1619182 41.623054 0.3038991 0.3803517 3.7858532 0.1701703
2 - 0.6123854 6.0033008 0.733945 4.2989963 82.469348 0.6670489 0.7912844 7.7129626 0.5428135
6 - 1.1284404 10.935019 1.5462538 8.9567306 171.49332 1.8902905 1.8138379 17.485072 2.4732242
10 - 1.6043578 15.483159 2.3681193 13.669262 261.56464 2.6930428 2.5783639 24.791322 2.6357034
15 - 2.5447248 24.469847 3.5053517 20.19009 386.1982 3.8685015 3.4862385 33.467494 3.7442661
Datos experimentales DNS (540nm). Concentraciones para hallar la actividad enzimática