PMID- 31356635

OWN - NLM
STAT- In-Data-Review
LR - 20190729
IS - 1932-6203 (Electronic)
IS - 1932-6203 (Linking)
VI - 14
IP - 7
DP - 2019
TI - Sodium butyrate interrupts the maturation of oocytes and enhances the
development
of preimplantation embryos.
PG - e0220479
LID - 10.1371/journal.pone.0220479 [doi]
AB - Histone acetylation is one of the most important posttranslational
modifications
that contribute to transcriptional initiation and chromatin remodeling. In
the
present study, we aimed to investigate the effect of sodium butyrate (NaBu),
a
natural histone deacetylase inhibitor (HDACi), on the maturation of oocytes,
preimplantation embryonic development, and expression of important
developmental
genes. The results indicated that NaBu decreased the rates of GVBD and the
first
polar body extrusion (PBE) in vitro in a dose-dependent manner. Meanwhile,
NaBu
treatment led to an abnormality in the spindle apparatus in oocytes in MI.
However, the ratio of phosphor-extracellular signal-regulated kinases (p-
ERK)/ERK
significantly decreased in oocytes treated with 2.0 mM NaBu for 8 h.
Furthermore,
NaBu treatment at 2.0 mM improved the quality of embryos and the mRNA
expression
levels of important developmental genes such as HDAC1, Sox2, and Pou5f1.
These
data suggest that although a high concentration NaBu will impede the meiosis
of
oocytes, 2.0 mM NaBu will promote the development of embryos in vitro.
Further
investigation is needed to clarify the direct/indirect effects of NaBu on the
regulation of important developmental genes and their subsequent impacts on
full-term development in mammals.
FAU - Yu, Meng-Fei
AU - Yu MF
AUID- ORCID: http://orcid.org/0000-0003-0937-5012
AD - Hubei Provincial Key Laboratory for Protection and Application of Special
Plant
Germplasm in Wuling Area of China, College of Life Sciences, South-Central
University for Nationalities, Wuhan, Hubei, China.
AD - Key Laboratory of State Ethnic Affairs Commission for Biological Technology,
College of Life Sciences, South-Central University for Nationalities, Wuhan,
Hubei, China.
FAU - Wang, Ju-Long
AU - Wang JL
AD - Key Laboratory of Agricultural Animal Genetic, Breeding, and Reproduction for
Ministry of Education, College of Animal Science, Huazhong Agricultural
University, Wuhan, China.
AD -
School of Biotechnology, Wuhu Institute of Technology, Wuhu, China.
FAU -
Yi, Jian-Ming
AU -
Yi JM
AD -
Key Laboratory of Agricultural Animal Genetic, Breeding, and Reproduction for
Ministry of Education, College of Animal Science, Huazhong Agricultural
University, Wuhan, China.
FAU - Ma, Lin
AU - Ma L
AD - Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical
Garden,
Chinese Academy of Sciences, Moshan, Wuchang, Wuhan, Hubei, China.
LA - eng
PT - Journal Article
DEP - 20190729
PL - United States
TA - PLoS One
JT - PloS one
JID - 101285081
SB - IM
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/07/30 06:00
MHDA- 2019/07/30 06:00
CRDT- 2019/07/30 06:00
PHST- 2019/04/02 00:00 [received]
PHST- 2019/07/16 00:00 [accepted]
PHST- 2019/07/30 06:00 [entrez]
PHST- 2019/07/30 06:00 [pubmed]
PHST- 2019/07/30 06:00 [medline]
AID - 10.1371/journal.pone.0220479 [doi]
AID - PONE-D-19-09283 [pii]
PST - epublish
SO - PLoS One. 2019 Jul 29;14(7):e0220479. doi: 10.1371/journal.pone.0220479.
eCollection 2019.