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`APPENDIX

I. Concentration of Standard Cu (II), ppm

M1V1 = M2V2

1. 2.00 mL working standard solution: 3. 6.00 mL working standard solution:

2500 ppm× 0.002 L 2500 ppm × 0.006 L

=100 ppm Cu(II) =300 ppm Cu(II )
0.050 L 0.050 L

2. 4.00 mL working standard solution: 4. 8.00 mL working standard solution:

2500 ppm× 0.004 L 2500 ppm × 0.008 L

=200 ppm Cu(II ) =400 ppm Cu(II )
0.050 L 0.050 L

5. 10.00 mL working standard solution:

2500 ppm × 0.010 L

=500 ppm Cu(II )
0.050 L

III. Calibration Curve

0.500
0.450 f(x) = 0x + 0
0.400 R² = 1
0.350
0.300
Absorbance

0.250
0.200
0.150
0.100
0.050
0.000
50.00 100.00150.00200.00250.00300.00350.00400.00450.00500.00550.00
Standard Cu(II) Concentration (ppm)

Figure 1. Standard Cu(II) Concentration versus Absorbance

III. Concentration of Diluted Sample

A−b 0.348−0.0001
x= A- Absorbance x= =386.56 ppm Cu( II )
m 0.0009
b- y-intercept of calibration
curve Trial 2:
m- slope of calibration curve
0.349−0.0001
x= =387.67 ppm Cu( II )
Trial 1: 0.0009

Trial 3:
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0.348−0.0001
x= =386.56 ppm Cu(II )
0.0009