J. A. Ines/Chemistry 26.

1 (2018) P a g e |3

`APPENDIX

I. Concentration of Standard Cu (II), ppm

M1V1 = M2V2

1. 2.00 mL working standard solution: 3. 6.00 mL working standard solution:

2500 ppm × 0.002 L 2500 ppm × 0.006 L

= 100 ppm Cu (II) = 300 ppm Cu (II)
0.050 L 0.050 L

2. 4.00 mL working standard solution: 4. 8.00 mL working standard solution:

2500 ppm × 0.004 L 2500 ppm × 0.008 L

= 200 ppm Cu (II) = 400 ppm Cu (II)
0.050 L 0.050 L

5. 10.00 mL working standard solution:

2500 ppm × 0.010 L

= 500 ppm Cu (II)
0.050 L
III. Calibration Curve

0.500
0.450
0.400
0.350 y = 0.0009x + 1E-04
Absorbance

0.300 R² = 0.9997
0.250
0.200
0.150
0.100
0.050
0.000
0.00 100.00 200.00 300.00 400.00 500.00 600.00
Standard Cu(II) Concentration (ppm)

Figure 1. Standard Cu(II) Concentration versus Absorbance

III. Concentration of Diluted Sample

x=
A−b
A- Absorbance Trial 2:
m
b- y-intercept of calibration x=
0.349−0.0001
= 387.67 ppm Cu (II)
curve 0.0009

m- slope of calibration curve Trial 3:

Trial 1: 0.348−0.0001
x= = 386.56 ppm Cu (II)
0.0009
0.348−0.0001
x= = 386.56 ppm Cu (II)
0.0009
J. A. Ines/Chemistry 26.1 (2018) P a g e |4