Manual Fitting: Sample Urea Denaturation of Lysozyme

Your Name Here

Change these Constants Minimize This Number

ΔG (kJ mol )-1
29.9102022909 Reduced X2 0.621
m (kJ mol-1 M-1) 9.3983217654

Don't Change These Constants

R (kJ mol-1 K-1) 8.31E-03
T (K) 298
af -0.231996
bf -6.55406
au 0.474099
bu -2.53758

Experimental Measurements Smooth Curve (Model)

Molar Ellipticity Uncertainty (Error
[Urea] (M) [Urea] (M) Molar Ellipticity
Bar)
0.00 -6.526 0.10 0.0 -6.55
0.25 -6.572 0.10 0.1 -6.58
0.50 -6.703 0.10 0.2 -6.60
0.75 -6.667 0.10 0.3 -6.62
1.00 -6.861 0.10 0.4 -6.65
1.25 -6.851 0.10 0.5 -6.67
1.50 -7.002 0.10 0.6 -6.69
1.75 -6.940 0.10 0.7 -6.72
2.00 -6.848 0.10 0.8 -6.74
2.25 -6.995 0.10 0.9 -6.76
2.50 -6.661 0.10 1.0 -6.78
2.75 -6.228 0.10 1.1 -6.81
3.00 -5.136 0.10 1.2 -6.83
3.25 -3.865 0.10 1.3 -6.85
3.50 -2.377 0.10 1.4 -6.87
3.75 -1.343 0.10 1.5 -6.89
4.00 -0.928 0.10 1.6 -6.91
4.25 -0.616 0.10 1.7 -6.93
4.50 -0.504 0.10 1.8 -6.94
4.75 -0.262 0.10 1.9 -6.95
5.00 -0.182 0.10 2.0 -6.96
5.25 -0.228 0.10 2.1 -6.95
5.50 0.164 0.10 2.2 -6.93
5.75 0.152 0.10 2.3 -6.90
6.00 0.336 0.10 2.4 -6.83
6.25 0.357 0.10 2.5 -6.73
6.50 0.585 0.10 2.6 -6.58
6.75 0.609 0.10 2.7 -6.36
7.00 0.874 0.10 2.8 -6.07
2.9 -5.68
3.0 -5.20
3.1 -4.65
3.2 -4.05
3.3 -3.45
3.4 -2.88
3.5 -2.37
3.6 -1.95
3.7 -1.60
3.8 -1.32
3.9 -1.11
4.0 -0.94
4.1 -0.80
4.2 -0.69
4.3 -0.60
4.4 -0.52
4.5 -0.45
4.6 -0.39
4.7 -0.33
4.8 -0.28
4.9 -0.23
5.0 -0.17
5.1 -0.12
5.2 -0.08
5.3 -0.03
5.4 0.02
5.5 0.07
5.6 0.12
5.7 0.16
5.8 0.21
5.9 0.26
6.0 0.31
6.1 0.35
6.2 0.40
6.3 0.45
6.4 0.50
6.5 0.54
6.6 0.59
6.7 0.64
6.8 0.69
6.9 0.73
7.0 0.78
7.1 0.83
7.2 0.88
7.3 0.92
7.4 0.97
7.5 1.02
7.6 1.07
7.7 1.11
7.8 1.16
7.9 1.21
8.0 1.26
 Urea Unfolding of a Protein
2.0

1.0

0.0

-1.0
Molar Ellipticity (x 103)
-2.0

-3.0

-4.0

-5.0

-6.0

-7.0 Data
Best
Fi t
-8.0
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0

Urea (M)
Manual Fitting: Your Guanidinium Denaturation of Lysozyme
Your Name Here

Change These Parameters Minimize This Number

y0 3.83E+02 Reduced X2 36952.087
a 148.981762366
b 6.5976063755
c 4.6855882083

Do Not Change This Calculation

calculated ymax 532.1085076916

Experimental Measurements Smooth Curve (Model)

Uncertainty (Error
[GndHCl] (M) FI 340 nm [GndHCl] (M) FI 340 nm
Bar)
2 390.4173584 0.10 0.0 383.13
2.5 387.5532227 0.10 0.1 383.13
3 379.7349548 0.10 0.2 383.13
4 420.0425415 0.10 0.3 383.13
4.5 455.0172729 0.10 0.4 383.13
6 497.6891479 0.10 0.5 383.13
7 528.9228516 0.10 0.6 383.13
0.7 383.13
0.8 383.13
0.9 383.13
1.0 383.13
1.1 383.14
1.2 383.15
1.3 383.16
1.4 383.18
1.5 383.21
1.6 383.25
1.7 383.31
1.8 383.40
1.9 383.51
2.0 383.67
2.1 383.87
2.2 384.14
2.3 384.48
2.4 384.91
2.5 385.45
2.6 386.12
2.7 386.95
2.8 387.95
2.9 389.16
3.0 390.59
3.1 392.29
3.2 394.26
3.3 396.54
3.4 399.15
3.5 402.10
3.6 405.39
3.7 409.04
3.8 413.02
3.9 417.33
4.0 421.93
4.1 426.78
4.2 431.84
4.3 437.06
4.4 442.38
4.5 447.75
4.6 453.09
4.7 458.37
4.8 463.53
4.9 468.53
5.0 473.34
5.1 477.92
5.2 482.25
5.3 486.33
5.4 490.15
5.5 493.70
5.6 496.99
5.7 500.03
5.8 502.82
5.9 505.38
6.0 507.73
6.1 509.87
6.2 511.83
6.3 513.61
6.4 515.22
6.5 516.70
6.6 518.03
6.7 519.25
6.8 520.35
6.9 521.35
7.0 522.26
7.1 523.09
7.2 523.84
7.3 524.52
7.4 525.14
7.5 525.71
7.6 526.22
7.7 526.69
7.8 527.12
7.9 527.51
8.0 527.86
 Guanidinium Unfolding of a Protein
600.0

500.0

Fluorescnce Intensity (AFU) 400.0

300.0

200.0

100.0
Lys ozyme
Data
Best Fi t
0.0
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0

Guanidinium HCl (M)