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Sanger sequencing is a method for DNA sequencing developed by Frederick Sanger in 1977. It involves using DNA polymerase to selectively incorporate chain-terminating dideoxynucleotides during in vitro DNA replication, generating DNA fragments of different lengths that can be separated by gel electrophoresis. The order of the bases is read from the gel according to the fragment lengths, allowing determination of the DNA sequence. Sanger sequencing revolutionized DNA sequencing and was the primary method of DNA sequencing until the development of next-generation sequencing technologies.
Sanger sequencing is a method for DNA sequencing developed by Frederick Sanger in 1977. It involves using DNA polymerase to selectively incorporate chain-terminating dideoxynucleotides during in vitro DNA replication, generating DNA fragments of different lengths that can be separated by gel electrophoresis. The order of the bases is read from the gel according to the fragment lengths, allowing determination of the DNA sequence. Sanger sequencing revolutionized DNA sequencing and was the primary method of DNA sequencing until the development of next-generation sequencing technologies.
Sanger sequencing is a method for DNA sequencing developed by Frederick Sanger in 1977. It involves using DNA polymerase to selectively incorporate chain-terminating dideoxynucleotides during in vitro DNA replication, generating DNA fragments of different lengths that can be separated by gel electrophoresis. The order of the bases is read from the gel according to the fragment lengths, allowing determination of the DNA sequence. Sanger sequencing revolutionized DNA sequencing and was the primary method of DNA sequencing until the development of next-generation sequencing technologies.