STANDARD OPERATION PROCEDURE
STANDARDIZATION OF SODIUM Revision No.
THIOSULFATE SOLUTION
1. Purpose
To determine the concentration of Thiosulfate
solution.
2. Responsible
Foreman and Asst Supervisor.
3. Apparatus
4.1 Analytical balance, accurate up to 0.0001 gram.
4.2 Methrom dosimat titrator with magnetic stirrer or
equivalent apparatus
4.3 Timer
4.4 Repeater pipette with filling flask, 20 milliliter.
4.5 Measuring pipette, 5 milliliter
4.6 Glass stoppered iodine flask, 250 millimeter.
4. Reagents
4.1 Kalium dikromat, reagent grade, roller to soft and
make to dry in temperature 110 degree C before
using minimal 2 hour.
4.2 Make Soluble kalium iodine, 10% maker with
soluble 10 gram KI (reagent grade) into 100
milliliter aquadest.
4.3 HCL reagent grade concentrated.
4.4 Make soluble starch 1% already check sensitivity
(if needed) make pasta from 1 gram starch and a
little aquadest. At the same time mixing added
100 milliliter boil water. Moved 5 milliliter the
solution into 100 milliliter aquadest and added
0.05 milliliter KI 0.1 the color dark blue disappear
be equal with adding 0.05 milliliter Na2Ss)3 0.1N.
5. Procedure
5.1 Weighing until 0.0001 gram a near, 0.1 +- 0.01
gram kalium dikromat into flask iodine 250
milliliter and added 25 milliliter aquadest. The
weight appropriate for Na2S2O3 o.1 N and mush
be appropriated with comparing 0.05 milliliter
Na2S2O3 0.1N.
5.2 Weighing 5 milliliter HCL concentrated and 20
milliliter kalium iodine solution. Stir in older than
mix.
5.3 So that 5 minute and added aquadest pass
through mouth of flask without release the closed.
5.4 Slowly, release the closed and continue adding
aquadest until total in used 100 milliliter included
volume in step 5.3.
5.5 Titration using Na2S2O3 solution an already
standardization with strong stirring and
continually until the color yellow near
disappeared.
5.6 Added 1 – 2 milliliter starch indicator and
continue titration with and thiosulfat solution in a
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SOP-QA-09-031
00
Effective Date 01.01.10
1. Tujuan
Menentukan konsentrasi larutan Thiosulfate.
2. Tanggung jawab
Foreman dan Asst. Supervisor.
3. Peralatan
4.1 Analytical balance, dengan akurasi 0.0001 gram.
4.2 Methrom dosimat titrator dengan magnetic stirrer
atau alat sejenis.
4.3 Alat Pengukur waktu.
4.4 Repeater pipette with filling flask, 20 ml.
4.5 Measuring pipette, 5 ml
4.6 Glass stoppered iodine flask, 250 ml.
4. Reagents
4.1 Kalium dikromat, reagent grade, digiling halus
dan dikeringkan pada 110 OC sebelum digunakan
min 2 jam.
4.2 Larutan kalium iodide, 10% dibuat dengan
melarutkan 10 gram KI (reagent grade) dalam
100 ml aquadest.
4.3 HCl reagent grade
4.4 Larutan kanji 1 %, telah di uji kepekaannya (jika
diperlukan) buat pasta dari 1 gram kanji dan
sedikit aquadest. Sambil diaduk tambahkan 100
ml air mendidih. Pindahkan 5 ml larutan ini ke
dalam 100 ml aquadest dan tambahkan 0.05 ml
KI 0.1 warna biru gelap harus hilang setara
dengan penambahan 0.05 ml Na2S2O3 0.1 N.
5. Prosedur
5.1 Timbang sampai 0.0001 gram terdekat, 0.1 ±
0.01 gram kalium dikromat ke dalam labu iodine
250 ml dan tambahkan 25 ml aquadest. Berat ini
memadai untuk Na2S203 0.1 N dan harus
disesuaikan dengan perbandingan berat yang
sama bila akan menstrandarisasi larutan standar
yang berbeda kosentrasinya.
5.2 Tambahkan 5 ml HCl dan 20 ml larutan kalium
iodide. Aduk agar bercampur.
5.3 Biarkan 5 menit dan tambahkan aquadest melalui
mulut labu tanpa melepas tutupnya.
5.4 Perlahan, lepaskan tutup tersebut dan lanjutkan
penambahan aquadest sampai total 100 ml
digunakan termasuk volume pada langkah 5.3.
5.5 Titrasi dengan larutan Na2S2O3 yang telah
distandarisasi dengan pengadukan yang kuat
terus menerus sampai warna kuning hampir
hilang.
5.6 Tambahkan 1-2 ml indicator kanji dan lanjutkan
titrasi dengan penambahan larutan thiosulfat
secara perlahan sampai warna biru tepat
 STANDARD OPERATION PROCEDURE SOP-QA-09-031

STANDARDIZATION OF SODIUM Revision No. 00

THIOSULFATE SOLUTION
Effective Date 01.01.10

slowly manner the color blue fix disappeared. menghilang.

6. Calculation 6. Perhitungan
N = W . N = W
0.04903 x V 0.04903 x V
Where :
N : Normalization Na2S2O3 solution N : Normalitas larutan Na2S2O3
W : Weight of Sodium dicromat (gram) W : Berat kalium dikromat (gram)
V : Milliliter of Na2S2O3 then needed in V : ml Na2S2O3 yang di butuhkan
filtration process. dalam titrasi

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