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The Microbial World

Question Answer
Define microbes/microorganisms A living organism too small to be seen with the naked eye.
Types of microbes (smallest to Viruses, bacteria, archaea, algae, fungi, protozoa, helminths (early stages)
largest)
Marine and freshwater form the basis of the food chain in oceans, lakes, and rivers
microorganisms function to...
Soil microbes function to.... break down wastes and incorporate nitrogen gas from the air into organic
compounds, thereby recycling chemical elements between the soil, water, life,
and air.
What is photosynthesis? a food and oxygen-generating process that is critical to life on Earth.
Humans and many other animals digestion in the intestines and for synthesis of some vitamins like vitamin B for
depend on microbes for... metabolism and vitamin K for blood clotting.
Medical commercial application of vitamins, organic acids, enzymes, alcohols, and many drugs.
microorganisms..
Military application of acetone helped to make cordite (a smokeless form of gunpowder) which played
microorganisms in WWI... a significant role in determining the outcome of WWI)
Food industry applications of producing vinegar, sauerkraut, pickles, alcoholic beverages, green olives, soy
microorganisms.... sauce, buttermilk, cheese, yogurt, and bread.
Pathogenic disease-producing
Genus (plural: genera) the first name used in scientific nomenclature assigned to each organism. It is
always capitalized.
Specific epithet (species) the second name used in scientific nomenclature assigned to each organism. It
is not capitalized.
Staphlo- describes the clustered arrangement of the cells
coccus indicates shape spherical or ovoid
aureus Latin for golden, the color of many colonies of this bacterium.
Escherichia coli is named after Honors Theodor Escherich whom discovered this bacteria. The specific epithet,
coli, reminds us that E. coli live in the colon, or large intestines.
Bacteria (singular: bacterium) relatively simple, single-celled (unicellular) organisms. Cell wall contains a
protein-carbohydrate complex called peptidoglycan; their genetic material is
not enclosed in a special nuclear membrane.
Prokaryotes Greek for prenucleus. Include both bacteria and archaea
Flagella appendages that allow many bacteria to "swim"
Archaea Consist of prokaryotic cells, if they have cell walls, the walls lack peptidoglycan;
found in extreme environments. Consist of Methanogens, Halophiles, and
Thermophiles
Methanogens one of three archaea that live in extreme environments; they produce methane
as a waste product from respiration. Not known to cause disease in humans.
Extreme halophiles halo = salt; philic one of three archaea that live in extreme salty environments such as the Great
= loving Salt Lake and the Dead Sea. Not known to cause disease in humans.
Extreme thermophiles therm = heat one of three archaea that live in extreme hot sulfurous water, such as hot
springs at Yellowstone National Park. Not known to cause disease in humans.
Fungi (singular: fungus) eukaryotic (contain distinct nucleus containing DNA), surrounded by nuclear
membrane. May be unicellular or multicellular. Cell walls are composed
primarily of chitin; can reproduce sexually or asexually.
Protozoa unicellular eukaryotic microbes; reproduce sexually or asexually
Algae (singular: alga) photosynthetic eukaryotes with a wide variety of shapes and both sexual and
asexual reproductive forms.
Viruses smallest form of microbes; most can only be seen with an electron microscope;
they are acellular; can only reproduce by using the cellular machinery of other
organisms. Thus they are considered living when they multiply within the cells
they infect.
Helminths parasitic worms (flatworms and roundworms); during some stage in their life
cycle, they are microscopic in size.
Cell theory all living things are composed of cells
Spontaneous generation a belief that some forms of life could arise spontaneously from nonliving matter
Francesco Redi set out to disprove spontaneous generation; using a controlled experiment.
what
Biogenesis the claim that living cells can arise only from preexisting living cells
Aseptic techniques technique that prevents contamination by unwanted microorganisms
Fermentation microorganisms called yeasts convert sugars to alcohol in the absence of air;
discovered by Pasteur
Pasteurization the process of heating solution enough to kill most of the bacteria that causes
spoilage and disease
Germ theory of disease the theory that microorganisms might cause disease
Koch’s postulates "set of rules"; which organisms cause which diseases
Immunity protection from disease provided by vaccination or previous infection
Chemotherapy the treatment of disease by using chemical substances
Antibiotics chemicals produced naturally by bacteria and fungi to act against other
microorganisms
Synthetic drugs chemotherapeutic agents prepared from chemicals in the laboratory
Bacteriology the study of bacteria; includes Gram Positive (Staph, Strep, Bacillus,) Gram neg
(E.coli, Salmonella,) Mycobacteria (TB and leprosy,) Spirochetes (Syphilis, Lyme
disease,) and others (Rickettsia, Chlamydia, Mycoplasma)
Mycology the study of fungi; includes the study of Fungi, Yeast (single cell), and Multi-
cellular (mushrooms and molds.) Used in medicine, agricultural, and ecological
branches.
Parasitology the study of protozoa and parasitic worms; Protozoans (Amoebas, Ciliates, and
Flageliates) and Helminths (flatworms and roundworms.)
Virology the study of viruses; Includes Acellular, DNA Viruses (Herpes, Pox viruses,) RNA
Viruses (Polio, rabies, influenza,) and Prions (Mad Cow disease)
Genomics the study of all of an organism’s genes
Immunology the study of immunity; Humoral (antibodies) and Cellular (Cytotoxic and killer
cells)
Recombinant DNA a form of artificial DNA that is created by combining sequences that would not
normally occur together through the process of gene splicing; it can be used to
mass produce desired proteins.
Microbial genetics studies the mechanisms by which microorganisms inherit traits
Microbial biology studies how genetic information is carried in molecules of DNA and how DNA
directs the synthesis of proteins
Bioremediation the process of using microbes to clean up pollutants and toxic wastes produced
by various industrial processes.
Biotechnology the practical application of using microorganisms commercial to produce
common foods and chemicals
Gene therapy inserting a missing gene or replacing a defective one in human cells
Normal microbiota normal flora, cause no harm and in some cases can be beneficial
What does EID stand for? emerging infectious diseases; examples include: bird flu ('05), monkeypox ('03),
SARS ('03), West Nile Fever ('01), Mad Cow Disease('96, Ebola (95),...HIV-AIDS
('78-'79)
Obtaining a pure culture of organism Koch's postulates
is the first step of which process
Agar a gelatinous substance derived from a polysaccharide that accumulates in the
cell walls of agarophyte red algae
Chitin a polysaccharide found in the outer skeleton of insects, crabs, shrimps, and
lobsters
Define transformation the process in which genes are transferred from one bacterium to another as
"naked" DNA solution.
What are Biofilms aggregated bacteria adhering to each other and to a solid surface
5 Disciplines of Microbiology 1. Virology 2. Bacteriology 3. Mycology 4.Parisitology and 5. Immunology
Prions are Infectious agent consisting of a self-replicating protein; an "infectious protein"

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