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INTRODUCTION
Medicinal plants are gaining much interest recently because their use in ethno
medicine treating common disease such as cold, fever and other medicinal claims are
now supported with sound scientific evidences. One is Aratiles (Muntingia calabura
way that it causes dizziness, fast heartbeat, weakness, fatigue, shaking and headache.
It can happen in people with diabetes who take medicines that increase insulin levels in
the body. Taking too much medication, skipping meals, eating less than normal or
exercising more than usual can lead to low blood sugar for these individuals.
Blood sugar which is also known as glucose comes from food and serves as an
important energy source for the body. Carbohydrates — foods such as rice, potatoes,
bread, tortillas, cereal, fruit, vegetables, and milk — are the body’s main source of
glucose.
After eating, glucose is absorbed into the bloodstream, where it travels to the body’s
cells. A hormone called insulin, which is made in the pancreas, converts glucose into
energy. If a person eats glucose more than he needs, the body will store it in the liver
and muscles or it will change it into fat so it can be used for energy when it is needed
later.
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Without enough glucose, your body cannot perform its normal functions. In the short
term, people who are not on medications that increase insulin have enough glucose to
maintain blood sugar levels, and the liver can make glucose if needed. However, for
those on these specific medications, a short-term reduction in blood sugar can cause a
lot of problems. Your blood sugar is considered low when it drops below 70 mg/dL.
Immediate treatment for low blood sugar levels is important to prevent more serious
Based from the Media Briefing on Nocturnal Hypoglycemia (2014), more than 382
million people worldwide have diabetes. Four million people have diabetes in the
Philippines. Half of them may experience nocturnal hypoglycemia. Almost 50% of all
hypoglycemic events happen at night. Up to 50% of adults with Type 1 diabetes may
suffer from nocturnal hypoglycemia. And up to 33% of adults with Type 2 diabetes may
The aim of this study was to test the effectiveness of Aratiles leaves in increasing
blood glucose level to help lessen the people who are suffering from hypoglycemia.
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Statement of the Problem
This study was conducted to prove the effectiveness of Aratiles (Muntingia calabura
d. Negative Control;
f. Untreated?
2. Are there significant differences on the effect of the different treatments prepared
that can increase blood glucose level in white albino wistar mice?
Research Hypotheses
prepared that can increase the blood glucose level in white albino wistar mice.
that can increase the blood glucose level in white albino wistar mice.
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Significance of the Study
The importance of this study is to be able to increase the blood glucose level of
individuals using Aratiles leaves. It is also significant since many citizens are suffering
from hypoglycemia. Eating candies, soda, and fruit juices are advisable for people who
have hypoglycemia, it takes time in order to finally increase their blood sugar. As a
remedy, the researchers found out that Aratiles leaves can increase the blood sugar
level in a fast manner without leaving any side effects that can worsen the said illness.
This study was conducted on June 2017- September 2017 at Juan G. Macaraeg
National High School and it was mainly focused on the property of Aratiles leaves that
The extraction of Aratiles leaves using ethanol and the acclimatization of animals,
inducing of the different treatments on six samples were all done at the said school.
Furthermore, the Aratiles leaves were used as the plant part, ethanol was used as
the solvent and white albino wistar mice as the test subjects. The researchers did not
use any other plants or plant parts other than Aratiles leaves, solvents other than ethanol
Hence, this research study only focused on the property of Aratiles leaves in
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Conceptual Framework
The concept of this study focused on Aratiles leaves in increasing the blood glucose
level of white albino wistar mice. The paradigm of the study shows the input, process,
and output of the study wherein in the input is the extraction of Aratiles leaves; process
is testing the effectivity of the negative control and extracted Aratiles leaves in different
concentrations; and output is the result in process that shows which concentration is the
most effective.
Concentrations of Aratiles
Leaves Ethanolic Extract
a. 60 mg/kg
b. 90 mg/kg Effective
Aratiles (Muntingia
calabura Linn.) Leaves concentration of
c. 120 mg/kg Aratiles Leaves
Ethanolic Extract
Ethanolic Extract
d. Pure Extract
e. Negative Control
f. Untreated
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Definition of Terms
Aratiles (Muntingia calabura Linn.) Leaves – can increase the blood glucose level
Ethanol – a solvent that was used in the study to dilute the extracted Aratiles leaves
Diabetes – can occur when the pancreas produces very little or no insulin, or when the
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CHAPTER II
Aratiles (Muntingia calabura Linn.) is a very popular tree among Filipino children
because of its small berry-rounded and pinkish when fruit was ripe. It can be seen
branches. It has a green, hairy and sticky leaves that measures 8 – 13 cm long with
tooth margins and pointed apex. Its leaves contain anti-oxidant, anti-microbial, anti-
septic, anti-spasmodic, flavones, flavonoids, and also provide nitric oxide. The flowers
on the tree last only a day, and their petals are usually gone by afternoon. It is
sometimes referred to as the Jamaican Cherry, or Strawberry Tree due to its blossom
The fruit requires a tropical climate and is indigenous to Central America, Southern
Mexico, tropical South America, as well as islands like Trinidad, St. Vincent, and
Jamaica. This fruit is also cultivated in regions with warm climates like Southeast Asia,
Constituents
flavonoid.
linoleic acid (31) palmitic acid (3b) and α-linolenic acid (3c), and a mixture of ß-
methanolic fruit extract yielded 1.49 g/100g gallic acid of phenolic content, 3
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mg/g CE of flavonoid, and 300 µg CGE/100g fresh mass fruit of anthocyanin.
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Properties
Use as Medicine
Although this fruit and its health benefits aren't familiar to most people, we want to
give you 10 reasons to look for it next time you're in the grocery store:
1. Antibacterial – this fruit consists of powerful antibacterial compounds that make the
Kerson cherry a great source of antibacterial agents. The fruit can even be used for
2. Gout – throughout history Kerson fruit has been used as an effective pain reliever for
gout. Eating 10-15 of these cherries can reduce or even eliminate pain.
3. Lowers blood pressure – making a tea of Kerson fruit leaves lowers blood pressure
due to nitric oxide that relaxes the blood vessels and improves blood flow.
also good for your heart. It aids in the prevention of heart attacks and other
inflammation.
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5. Helps with headaches – eating the fruit or making a tea from its leaves can provide
significant relief for headaches. It is often recommended for people suffering from
6. Pain reliever – we mentioned that Kerson cherries relieve pain associated with gout
and headaches. However, eating the cherries or drinking the tea from its leaves can
help relieve all sorts of aches and pains in your body due to its natural ability to block
pain receptors. This means that you don’t have to rely on pharmaceutical drugs and
7. Prevents cancer – studies have confirmed that Kerson leaves contain cancer fighting
abilities, and they may be used as a key component in the future of cancer treatment.
Scientists have emphasized the importance of more research in order to confirm the
8. Anti-inflammatory purposes – eating the cherries and drinking the tea can also be
9. Helps with abdominal cramps – the fruit and tea has a remarkable ability to help
10. Helps with diabetes – Kerson cherries are also known to lower blood sugar, making
11. It is a huge source of Vitamin C – One hundered grams of the berries is equal to
150 mgs of Vitamin C. We already know that this vitamin helps to prevent flu and
colds, has strong antioxidants, and even works to improve some types of
cardiovascular disease.
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12. It has strong antioxidant properties – Oxidation is a chemical process in your body
that releases free radicals. These damaging free radicals form chains and are
considered to be culprits in cancer and other diseases. Antioxidants kill free radicals,
and Kerson Fruit is full of them — 24 different flavonoids and phenolic compounds to
be exact. Don’t worry too much about exactly what flavonoids and phenolic
compounds are — you just need to know that they are the “good guys.”
13. It contains important nutrients – The fruit itself includes fiber, carbs, protein,
calcium, phosphorous, iron, and B-vitamins (which are some great mood enhancers).
With this type of excellent nutritional benefit, it is no wonder that Kerson Fruit is such
a staple in other parts of the world. People eat the fruit raw, make jams and jellies,
and use it for baking. Finding Kerson Fruit in the US is a bit challenging. You can,
however, buy it online in liquid form or in form of one or a group of supplements, and
Use as Food
The fruit is generally consumed in three different ways: directly from the tree, in a
Others
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Low blood sugar can occur for a number of reasons. It’s usually a side effect of
diabetes treatment.
Diabetes affects the body’s ability to use insulin. Think of insulin as the key that
unlocks your cells, letting glucose in for energy. People with diabetes use a variety of
treatments to help their bodies use the glucose in their blood. Among these are oral
If you take too much of these types of medications, your blood sugar may drop too
low. People also sometimes experience low blood sugar when planning to eat a big
meal, but then they do not eat enough. Skipping meals, eating less than normal, or
eating later than normal but taking your medication at your normal time can also lead to
Unplanned excess physical activity without eating enough can also cause a drop in
Drinking alcohol when you’re on these medications can also lead to low blood
sugar, especially if it replaces food. When the body is trying to get rid of alcohol it
You don’t have to have diabetes to experience low blood sugar. Some other causes
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Symptoms of mild low blood sugar
You may have these symptoms when your blood sugar has dropped below 70 milligrams
per deciliter (mg/dL). When you have had diabetes for many years, you may not always
Some young children with diabetes cannot recognize symptoms of low blood sugar. Others
can, but not every time. To be safe, the parents need to do a home blood sugar test
Sweating (almost always present). Check for sweating on the back of your neck at
your hairline.
Blurred vision.
These symptoms may go away shortly after you eat food that contains sugar.
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Chapter III
METHODOLOGY
Research Design
This research study aimed to determine the property of Aratiles leaves in increasing
blood glucose level. Experimental method design will be used in this study.
In this experiment, white albino wistar mice were divided into 6 groups.
Table 1
Concentrations
REPLICATES CONCENTRATION
OF
TREATMENTS
1 60 mg/kg
2 90 mg/kg
3 120 mg/kg
4 Pure Extract
5 Negative Control
6 Untreated
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Table 1: Three concentrations; untreated, pure Aratiles leaves extract and negative
3. Group of mice given a treatment of 120 mg/kg Aratiles leaves ethanolic extract
Table 2
DOSAGE (ml)
Table 2: The dosage of Aratiles leaves ethanolic extract that were administered in mice.
The given data above uses millilitre (ml) as the unit for measurement because the
aratiles leaves extract were diluted by ethanol.
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Set Up
This study had three trials and three set ups for control group; pure Aratiles leaves
extract for the first set up; for the negative control, ethanol solvent was used; and the
For the experimental group, there were three trials in three set ups. Set up A was 60
mg/kg; Set up B was 90 mg/kg; and Set up C was 120 mg/kg. The independent variable
in this study was the amount of Aratiles leaves extract given in mice while the dependent
variable was the change in blood glucose level of mice. Controlled variable was the mice
1. Plant Material
the leaves were cut into tiny pieces which weighed 200 grams. Next, the
leaves were put in an Erlenmeyer flask and added with a 200 ml ethanol
and locked using a cork. Lastly, it was macerated for 72 hours and it was
2. Experimental Animals
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Welfare Act of 1998” in the experiment. Department of Agriculture
Before being used in the experiment, white albino wistar mice were
being adapted for 7 days in order to get used to the environment. The
acclimatization, the mice were given a standard pellet for mice 3 times a
day (1 tbsp.) and 75 ml of water every day. Their cages were cleaned
every after two days and changed with clean sawdust bedding.
b) Procedures
Administration of Treatment
Wistar Mice based on Calculating Injection Doses for Rodents cited by Research
Animal Resource Center (2017). For the greatest accuracy in dosing, each
individual animal is weighed, and that result is used for the equation:
was required.
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Evaluation of Hyperglycemic Property
The researchers gathered a droplet of blood on the tail tip of each mouse
and their blood glucose were monitored using a glucometer. Prior to treatments
with plant extract, the researchers checked and recorded the blood glucose level
c) Extraction Procedure
First, the researchers gathered and washed Aratiles leaves. Then, the leaves
were cut into tiny pieces which weighed 200 grams. Next, the leaves were put in an
Erlenmeyer flask and added with a 200 ml ethanol and locked using a cork. Lastly, it
was macerated for 72 hours and it was evaporated using a hot plate stirrer.
The Aratiles leaves extract was tested for the presence of phytochemical
compounds.
warming on steam bath for 1 or 2 minutes. The extract was called, filtered, and
then adjacent the volume of the filtrate to 7 ml by washing the residue on filter
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The 1 ml of the filtrate was placed into each 4 small test tubes. To the first
test tubes, 3 drops of Modified Mayer’s Regent (Mercury Potassium Iodide TS)
was added, as the second test tube, 3 drop of Mayer’s Regent (Mercury Iodide
TS) was added, to the third, 3 drops of Wagner’s Regent (Iodide and Potassium
TS) and the last test tube, 3 drops of Bouchard’s regent (2% Iodide and 4%
Potassium Iodide).
bath. The residue was cooled to a room temperature and 15 ml of light petroleum
ether was added. Mixed well and filtered. The procedure was repeated with
where combines. The defatted residue was set aside for screening of flavonoids
and leucoanthocyanins.
The combination ethereal filtrate was evaporated to dryness and the residue
anhydrous sodium sulphate, filtrated, and divided the filtrate equally into three dry
test tubes. The following tests were essentially dehydration reaction and
The defatted residue was added from section B-3 in 30 ml of 50% ethanol
The evaporating dish was removed from the stem bath 25ml of hot distilled water
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By mixing it well with a steering rod allowed it to cool at room temperature
spontaneously. The cooled extract was centrifuged for several minutes and the
upper half decanted from each tube used. 3-4 drops of 10% sodium chloride
precipitate was filtered. 3 ml of filtrated each of the tree tubes was added.
Treatment Amount
A 60 mg/kg
B 90 mg/kg
C 120 mg/kg
D Pure Extract
E Negative Control
F Untreated
f) Treatment of Data
The result of the blood glucose level of mice with different concentrations,
positive and negative control, and untreated, using the analysis of variance and
statistical analysis using Median test p<0.05 was used in considering the
significant.
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Experimental Flowchart
Phytochemical Sreening
Interpretation of Results
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