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1/Introduction:
Carbohydrates are the most abundant class of organic compounds found in plant
and animal cells. They originate as products of photosynthesis. The formulas of
many carbohydrates can be written as CnH2nOn or (CH2O)n .Carbohydrates are
divided into monosaccharides ,disaccharides , polysaccharides.
2/Procedure
3. Collect the scratching and place on the slide, add a water drop and cover with
coverslip. Observe under microscope
4. After adding1-2 drops of Lugol solution to the edge of the coverslip and observe
the phenomenon
2. Take out 1 drop of starch suspension and put onto the glass side. Add 1 drop of
Lugol solution and using toothpick to mix. Observe the color change of this
suspension (compare with the Lugol solution’s color)
3. Add 5 drops of concentrated HCl solution into that test tube. Mix well
4.Take out 1 drop of starch-HCl mixture onto the slide and test the color with 1
drop of Lugol solution
5. Place the test tube containing starch and HCl into the laboratory water bath
(100oC) and boil this suspension. Every 2 minutes, take out 1 drop of the mixture
and put onto a glass side, let it cool down for a while and add 1 drop of Lugol
solution. Observe the change of color intensity.
6. Coninue boil and test with Lugol solution until no color change is detected.
Mark the time that the color does not change
3/ Result:
a)Microscopic observation:
Lugol solution
-Add Lugol into the mixture of starch and HCl, HCl makes the polymers chain of
starch shorter but we still got deep blue solution, like the previous step
-When we put test tube of HCl and starch into hot water bath, bond between starch
and iodine broke. Add 1 drop of Lugol solution into the mixture after every 2
minutes , we can see that the color slowly change from deep blue to dark brown
and finally is the amber color of Lugol solution
Color of spot
Lugol Amber
8 min Brown
4/ Discussion:
b) Explain the different color in starch- HCl mixture after time of boiling.
Based on the color of spot, why we can say that the structure of starch is
affected by temperature
Starch includes chains of monomers as glucoses. These monomers bonded to
each other by glycosidic linkage. When we add HCl , the polymers chain of
starch becomes shorter. After adding Lugol solution, starch and iodine
combines with each other. However, under the temperature of hot water bath
, the conformation of iodine is no longer lasting. As a result, the color
changes from deep blue to light brown