Peptide mapping

Peptide mapping, the principal technique for confirming a protein’s primary

structure (amino acid sequence), can be used in drug discovery and
throughout the manufacturing process. It is one of the most important
techniques in comparability studies of biologics and a key step in the
characterization of biopharmaceutical proteins. For recombinant protein drugs,
such as monoclonal antibodies (mAbs) and antibody drug conjugates (ADCs),
peptide mapping can be used for identification, primary structural
characterization, and quality assurance/quality control (QA/QC). Some global
regulators, like the US Food and Drug Administration (US FDA) and the
European Medicines Agency (EMA), have developed guidelines to ask for
providing peptide mapping to confirm the amino acid sequence of drug
substance proteins.

Peptide Mapping Service at Creative Proteomics

At Creative Proteomics, we have developed a liquid chromatography with

tandem mass spectrometry (LC-MS/MS)-based peptide mapping platform for
confirmation of protein primary structure. And we can provide the peptide
mapping service to analyze the sequence of biopharmaceuticals and confirm
complete expression of recombinant protein in compliance with ICH Q6B.

Figure 1. Workflow of Peptide Mapping Service at Creative Proteomics

As peptide mapping service compares the peptide masses with protein

databases, only proteins from the database can be identified using this method.
Proteins, whose sequence is not found in a protein database, such as
antibodies or novel proteins, can be deduced by our service of de-novo
sequencing. In addition, we can provide detailed reports, including:

Experiment procedures
Parameters of liquid chromatography and mass spectrometer
MS raw data files
Bioinformatics analysis
Peptide mapping results
Our Advantages

Various types of proteases used to achieve coverage of 100%, such as trypsin,

chymotrypsin, Asp-N, Glu-C, Lys-C, and Lys-N.
Professional peptide mapping platform including advanced instrument and
software.
Protein sample 100% sequence validation service.
Delivery of detailed report in a fast turnaround time.
Technology Platform

2D Electrophoresis
High Performance Liquid Chromatography (HPLC)
Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS)
Points of Interest in Peptide Mapping Services
Our peptide mapping service can help clients to comply with ICH Q6B
guidelines in support of marketing applications. At Creative Proteomics,
peptide mapping services have different uses, including but not limited:

Amino acid sequence. Peptide mapping is a valuable method to confirm the

amino acid sequence of purified proteins, like drug substance protein, in
accordance with ICH Q6B.
Post-translational modifications (PTMs). Based on peptide mapping, we can
analysis PTMs, including deamidation, oxidation, N-terminal pyroglutamic acid,
C-terminal lysine clipping, and glycosylation.
Disulfide bridges and free thiols. At Creative Proteomics, peptide mapping can
be performed without reduction to determine the positions of disulfide bridges
and/or free thiols (sulfhydryl groups).
With professional and experienced staff, Creative Proteomics provides
high-quality peptide mapping services. Our peptide mapping service is not only
for structural characterization and confirmation, but provides a whole suite of
information to help inform the drug development process. Our ordering
procedure is as follows. As every project has different requirements, please
contact our specialists to discuss your specific needs.

Ordering Procedure

Our customer service representatives are available 24 hours a day, from

Monday to Sunday.