DONOR’S 3% - 5% WASHED RED CELL SUSPENSION PATIENT’S 3% - 5% WASHED RED CELL SUSPENSION

MAJOR CROSS-MATCHING AUTO-CONTROL

1 gtt of patient’s plasma 1 gtt of patient’s plasma
1 gtt of donor’s suspension 1 gtt of patient’s suspension
centrifuge for 1 minute centrifuge for 1 minute
mix mix
observe observe

add 1 gtt of Low Ionic Strength Solution (LISS) add 1 gtt of Low Ionic Strength Solution (LISS)
mix gently mix gently
incubate for 15 – 20 minutes incubate for 15 – 20 minutes
centrifuge for 1 minute centrifuge for 1 minute
mix mix
observe observe

wash 3 times with Normal Saline Solution (NSS) wash 3 times with Normal Saline Solution (NSS)

blot blot
add 1 gtt of Anti-Human Globulin (AHG) add 1 gtt of Anti-Human Globulin (AHG)
centrifuge for 1 minute centrifuge for 1 minute
mix mix
observe observe
FORWARD TYPING
ANTI-A ANTI-A
1 gtt of anti-A antiserum 1 gtt of anti-A antiserum
1 gtt of donor’s red cell suspension 1 gtt of patient’s red cell suspension
centrifuge for 1 minute centrifuge for 1 minute
mix and observe mix and observe
ANTI-B ANTI-B
1 gtt of anti-B antiserum 1 gtt of anti-B antiserum
1 gtt of donor’s red cell suspension 1 gtt of patient’s red cell suspension
centrifuge for 1 minute centrifuge for 1 minute
mix and observe mix and observe
ANTI-D ANTI-D
1 gtt of anti-D antiserum 1 gtt of anti-D antiserum
1 gtt of donor’s red cell suspension 1 gtt of patient’s red cell suspension
centrifuge for 1 minute centrifuge for 1 minute
mix and observe mix and observe
REVERSE TYPING
KNOWN A KNOWN A
1 gtt of known A cells 1 gtt of known A cells
1 gtt of donor’s plasma 1 gtt of patient’s plasma
centrifuge for 1 minute centrifuge for 1 minute
mix and observe mix and observe
KNOWN B KNOWN B
1 gtt of known B cells 1 gtt of known B cells
1 gtt of donor’s plasma 1 gtt of patient’s plasma
centrifuge for 1 minute centrifuge for 1 minute
mix and observe mix and observe