Cell Culture Guidelines

TrypLE Seeding Density

Surface
Vessel Size Media Volume* Express (suggested)
area (cm2)
Volume
Plates (#/well)
6-well 9.5 1.9-4.8 mL .5 mL 2.9 x 105
12-well 3.8 .8-1.9 mL .3 mL 1.1 x 105
24-well 1.9 .4-1 mL 200 μL 5.7 x 104
48-well .95 190-475 μL 160 µL 2.9 x 104
96-well .32 72-180 µL 40 µL 9.6 x 103
Flasks
T25 25 5-12.5mL 1 mL 7.5 x 105
T75 75 15-37.5mL 2 mL 2.3 x 106
T150 150 30-75mL 4 mL 4.5 x 106
Dishes
35mm dish 8.8 1.8-4.4mL .6 mL 2.6 x 105
60mm dish 21.5 4.3-10.8 mL 1 mL 6.3 x 105
100mm dish 55 11-27.5 mL 1.5 mL 1.7 x 106
150mm dish 150 30-75 mL 415 mL 4.5 x 106
*Numbers calculated utilizing CO2 penetration equation (0.2-0.5 x cm2)

Cell Line Name Cell line origin Origin Species Media

12D7-MDR CD4+ T-lymphocyte Homo sapiens RPMI-1640
1720-CRL Testis Mus musculus DMEM
A-375 Skin Homo sapiens DMEM
A549 Lung Homo sapiens F-12K Medium
4T1 Mammary Gland Mus musculus RPMI-1640
BaF3 Murine Bone Marrow Mus musculus RPMI-1640
C166-GFP Yolk sac Mus musculus DMEM
C2C12 Muscle Mus musculus DMEM
CHO-K1/CCL-61 Ovary Cricetulus griseus F-12K
Colo-205 Colon Homo sapiens RPMI-1640
COS-7 Kidney Cercopithecus DMEM
aethiops
D835Y-LUC Acute Myeloid Leukemia Homo sapiens RPMI-1640
DMS 114 Lung Homo sapiens Waymouth’s MB
DU 145 Prostate Homo sapiens MEM
H1581 Lung Homo sapiens RPMI-1640
H1703 Lung Homo sapiens RPMI-1640
H1755 Lung, derived from Homo sapiens RPMI-1640
metastatic liver
H460 Lung, pleural effusion Homo sapiens RPMI-1640
H520 Lung Homo sapiens RPMI-1640
H9 Cutaneous T Lymphocyte Homo sapiens RPMI-1640
HCC827 Lung Homo sapiens RPMI-1640
hCMEC/D3 Blood-Brain Barrier Homo sapiens EndoGRO -MV
Cell Line Name Cell line origin Origin Species Media
HCT 116 Colon Homo sapiens McCoy’s 5a Medium
HCT15 Colon Homo sapiens RPMI-1640
HEK-293 Embryonic Kidney Homo sapiens MEM
HeLa Cervix Homo sapiens DMEM
HEL92.1.7 Bone Marrow Homo sapiens RPMI-1640
HepaRG Hepatic progenitors Homo sapiens Williams’ Medium E
Synovial tissue with
HFLS-OA Homo sapiens HFLS Growth Medium
osteoarthritis
HL60 Peripheral Blood Homo sapiens Iscove’s Modified DM
HLY-1 B-cell Homo sapiens
HOS Bone Homo sapiens MEM
HT1080 Connective Tissue Homo sapiens Eagle’s MEM
HT29 Colon Homo sapiens McCoy’s 5a Medium
HUVEC Umbilical vein Homo sapiens F-12K
ISG-KO-STING Melanoma Homo sapiens RPMI
J774A.1 Macrophage Mus musculus DMEM
K-562 Bone marrow Homo sapiens Iscove’s Modified DM
Kasumi-1 Myeloblast Homo sapiens RPMI-1640
KB HeLa contaminant Homo sapiens MEM
KG-1 Bone marrow Homo sapiens Iscove’s Modified DM
KO cGAS THP-1 Monocytes Homo sapiens RPMI-1640
L1210 Skin Lymphoblast Mus musculus DMEM
L-929 Subcutaneous connective Mus musculus MEM
tissue
LC-2-AD Lung Homo sapiens RPMI 1640
LNCAP Prostate Homo sapiens RPMI-1640
M109 Murine lung carcinoma Mus musculus RPMI-1640
MB49 Bladder Mus musculus
MDA-MB-231 Mammary Gland Homo sapiens Leibovitz’s L-15
McCoy Fibroblast Mus musculus MEM
MCF-7 Mammary Gland Homo sapiens DMEM
MCR-5 Lung Homo sapiens MEM
MDCK Kidney Canis familiaris MEM
MG-63 Bone Homo sapiens MEM
MIA PaCa Pancreas Homo sapiens DMEM
MOLM 14 Myeloblast Homo sapiens RPMI-1640
MOLM 13 Acute Myeloid Leukemia Homo sapiens
MV-4-11 Lymphoblast Homo sapiens Iscove’s Modified DM
NCI-H460 Lung Homo sapiens RMPI-1640
NIH3T3 Embryo Mus musculus DMEM
NOMO1 Hemo-lymphocytic Homo sapiens
OVCAR-3 Ovary Homo sapiens RPMI-1640
PC-3 Prostate Homo sapiens F-12K Medium
PK1(LLC) Kidney Sus scrofa Medium 199
 Cell Culture Guidelines
Cell Line Name Cell line origin Origin Species Media
RAW 264.7 Abelson murine leukemia Mus musculus DMEM
virus-induced tumor
RAW Blue Macrophages Mus musculus DMEM
RBL-243 Peripheral blood Rattus norvegicus MEM
RS4;11 Bone Marrow Homo sapiens RPMI-1640
Saos-2 Bone Homo sapiens McCoy’s 5A Medium
SKOV3 Ovary Homo sapiens McCoy’s 5A Medium
Stomach, derived from
SNU16 Homo sapiens RPMI-1640
metastatic ascites
T24 Urinary Bladder Homo sapiens McCoy’s 5a Medium
THP-1 Monocyte Homo sapiens RPMI-1640
TT Thyroid/Medulla Homo sapiens F-12K Medium
U-87 Brain Homo sapiens MEM
UMUC3 Urinary Bladder Homo sapiens MEM