Analytical Assay for Glycerol

Name of Chemical Molecular Formula

Glycerol C3 H8 O3

Parameters Specifications

1) Appearance. Syrup Liquid

2) Colour Colour or almost colourless
3) Solubility in H2o . Miscible
4) Identification. +ve
5) Refraction Index. 1.470-1.475
6) Specific gravity at 25C. NLT1.249
7) Solubility in Ether Insoluble

Description Notes For Procedure:

1) Identifications :

Mix 1 ml with 0.5 ml of HNO3 superimpose 0.5 ml of potassium dichromate solution a blue ring
develops at the interface of the two liquids allow stand for 10 minutes. The blue colour does not
diffuse at the lower layer

2) Ref Index:

Ref index is determined by refract meter .and specific gravity is determined by RD bottle.