0 valutazioniIl 0% ha trovato utile questo documento (0 voti)
164 visualizzazioni16 pagine
The document describes a student research project on the anti-inflammatory properties of Muntingia calabura (Aratilis) leaf extract. It lists the group members and research title. It then provides 3 examples of journal articles about Muntingia calabura and its properties: 1) discusses using its leaves to create an anti-inflammatory ointment, 2) identifies new cytotoxic flavonoids isolated from its roots, and 3) examines its effects on isoproterenol-induced myocardial infarction in rats.
The document describes a student research project on the anti-inflammatory properties of Muntingia calabura (Aratilis) leaf extract. It lists the group members and research title. It then provides 3 examples of journal articles about Muntingia calabura and its properties: 1) discusses using its leaves to create an anti-inflammatory ointment, 2) identifies new cytotoxic flavonoids isolated from its roots, and 3) examines its effects on isoproterenol-induced myocardial infarction in rats.
The document describes a student research project on the anti-inflammatory properties of Muntingia calabura (Aratilis) leaf extract. It lists the group members and research title. It then provides 3 examples of journal articles about Muntingia calabura and its properties: 1) discusses using its leaves to create an anti-inflammatory ointment, 2) identifies new cytotoxic flavonoids isolated from its roots, and 3) examines its effects on isoproterenol-induced myocardial infarction in rats.
Group Members: Calamba, Micah Lou C. Aragon, Jerielle Jade T. Jakosalem, Rhem Catherine
Research Title: Aratilis (Muntingia calabura) leaf extract as Anti-inflammatory ointment
Plant common name:Aratilis/Aratiles, Manzanitas, Sarisa (Bacolod area)
No Journal title Method Use
. 1. Aratilis ointment The raw material is the herbal plant, Aratilis which will become a form of ointment after processing. Aratilis (Muntingia Calabura known in the Philippines as “aratilis”, “aratiles” or “saresa”) is a widely cultivated fruit-bearing tree which is abundant in tropical countries such as the Philippines. It strives in soil despite of acidity rates that most plants can’t survive on and normally grows in roadsides, open grasslands, mountains, and backyards- almost anywhere in every province in the archipelago. It is a small tree 7– 12 meters tall with tiered and slightly drooping branches. It has serrated leaves 2.5–15 cm long and 1–6.5 cm wide. The flowers are small, white and slightly malodorous. It gives rise to 1–1.5 cm light red fruit. The fruit is edible, sweet and juicy, and contains a large number of tiny (0.5 mm) yellow seeds. It is a pioneer species that thrives in poor soil, able to tolerate acidic and alkaline conditions and drought. Its seeds are dispersed by birds and fruit bats. It is cultivated for its edible fruit, and has become naturalized in some other parts of the tropics, including southeastern Asia. As a pioneer plant, it could help condition the soil and make it habitable to other plants. However, it might also be considered as an invasive species since it might out-compete indigenous plants. 2. Plant anticancer agents, XLVIII. New cytotoxic flavonoids From a cytotoxic Et2O-soluble from Muntingia calabura root s. extract of Muntingia calabura roots, twelve new flavonoids were isolated, constituting seven flavans 1-7, three flavones 8, 10, and 12, and two biflavans 9 and 11. The structures of compounds 1-12 were established by the interpretation of spectral data, with the nmr assignments of these constituents being based on 1H-1H COSY, 1H-13C HETCOR, and selective INEPT experiments. This is the first report of the occurrence of 7,8-di-O- substituted flavans, biflavans, and flavones. Most of the isolates demonstrated cytotoxic activity when tested against cultured P-388 cells, with the flavans being more active than the flavones. Furthermore, certain of these structurally related flavonoids exhibited somewhat selective activities when evaluated with a number of human cancer cell lines. 3. Effects of Muntingia calabura L. on Six groups of Wistar albino rats, isoproterenol-induced myocardial each infarction comprising six animals, were Commented [1]: selected for this study. Group I served as a control, Group I I rats were given isoproterenol (20 mg/ 100g, subcutaneously), and Group Ill rats were given M. calabura leaf extract (300 mg/kg). Groups IV, V and VI rats were given M. calabura leaf extract (100 mg/kg, 200 mg/kg and 300 mg/kg, respectively) and isoproterenol (20 mg/100g subcutaneously) prior to MI induction. The transaminases (aspartate transaminase and alanine transaminase), lactate dehydrogenase (LDH) and creatine phosphokinase (CK), were estimated in both the serum and heart tissues, and the serum uric acid level was also estimated. 4 The extract was prepared by The Antinociceptive Action of Aqueous Extract soaking the dried powdered from Muntingia calabura Leaves: The Role of Opioid leaves of M. calabura in distilled Receptors water (dH2O) overnight, and the supernatant obtained was considered as a stock solution with 100% concentration. The stock solution was diluted to 1, 5, 10, 50 and 100% and used to determine the antinociceptive activity of MCAE. A further experiment was done with 50% concentration to determine the effect of temperature and naloxone involvement of the opioid receptor system in MCAE antinociceptive activity. Results: At the various concentrations MCAE showed significant antinociceptive activity in both tests. However, the concentration-dependent activity was observed only in the ACT but not in the HPT. The 50% concentration of MCAEs were also stable against the effect of various temperatures as indicated by the presence of activity in both tests. The temperatures (40, 60 and 100°C) also showed an enhanced extract activity only in the HPT. Pre- treatment with naloxone (2 and 10 mg/kg) blocked the extract activity in both tests, indicating the involvement of the opioid receptor system in MCAE antinociceptive activity. 5 This study dealt with the anti- Anti-platelet aggregation activity of the fresh ripe fruits of platelet aggregation activity of Muntingia calabura linn. (manzanitas) fresh ripe fruit of Muntingia calabura Linn. (mazanitas). Manzinatas are known to have antiseptic, astringent and antispasmodic properties. Study also shows bioactivity of manzanitas which includes antibacterial property. Fresh ripe fruits of manzanitas were used in the study. Methods of extraction included expression and masceration. Five different test solutions with 100mg/mL concentrations were used. There were five test solutions used in the Giemsa Micoplate Assay, test solution 1 (Pure Manzanitas Juice), test solution 2 (CrudeEthanol-free), test solution 3 (Hexane-free), test solution 4 (Ethyl acatate-free) and test solution 5 (Ethanol-free). Aspirin solution with 2 mg/mL concentration and dimethyl sulfoxide (DMSO) were used as positive and negative controls, respectively. Giemsa Microplate assay was used in the determination of the anti- platelet aggregation activity. Three trials were conducted in quadraplicates. The test determination of the anti- platelet aggregation was subjected to phytochemical screening using thin layer chromatography. Out of the five test solutions , only test solution 5 (Ethanol-free) inhibited platelet aggregation at concentration of 100mg/mL as shown by the absence of violet gels using the Giesma Microplate Assay. Test solution 1-4 did not inhibit platelet aggregation. In the phytochemical screening, it was found out that the ethanol-free extract contain flavonoids and tannins. The ripe fruits of manzanitas had anti-platelet aggregation activity using the Giesma Microplate Assay. It is recommended that all the test solutions must undergo phytochemical screening to determine the active constituents present. Cell counting of the aggregated platelets should also be done in order to have analysis of the anti- platelet. 6 The in vitro antiproliferative and In vitro antiproliferative and antioxidant activities of the antioxidant activities of the extracts of Muntingia calabura leaves. aqueous, chloroform and methanol extracts of Muntingia calabura leaves were determined in the present study. Assessed using the 3,(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay, the aqueous and methanol extracts of M. calabura inhibited the proliferation of MCF-7, HeLa, HT- 29, HL-60 and K-562 cancer cells while the chloroform extract only inhibited the proliferation of MCF-7, HeLa, HL-60 and K-562 cancer cells. Interestingly, all extracts of M. calabura, which failed to inhibit the MDA-MB-231 cells proliferation, did not inhibit the proliferation of 3T3 (normal) cells, indicating its safety. All extracts (20, 100 and 500 μg/ml) were found to possess antioxidant activity when tested using the DPPH radical scavenging and superoxide scavenging assays with the methanol, followed by the aqueous and chloroform, extract exhibiting the highest antioxidant activity in both assays. The total phenolic content for the aqueous, methanol and chloroform extracts were 2970.4 ± 6.6, 1279.9 ± 6.1 and 2978.1 ± 4.3 mg/100 g gallic acid, respectively. In conclusion, the M. calabura leaves possess potential antiproliferative and antioxidant activities that could be attributed to its high content of phenolic compounds, and thus, needs to be further explored. 7 Activation of Nitric Oxide Signaling Pathway The cardiovascular effect of the Mediates Hypotensive Effect of Muntingia crude methanol extract from the leaf of Muntingia calabura L. (Tiliaceae) Leaf Extract calabura L. (Tiliaceae) was investigated in the anesthetized rats. The crude methanol extract was sequentially fractionated to obtain the water-soluble extract (WSE). Intravenous administration of the WSE (10, 25, 50, 75 or 100 mg/kg) produced an initial followed by a delayed decrease in systemic arterial pressure (SAP) in a dose-dependent manner. The M. calabura- induced initial hypotension lasted for 10 min and the delayed depressor effect commenced after 90 min and lasted for at least 180 min post-injection. The same treatment, on the other hand, had no appreciable effect on heart rate (HR) or the blood gas/electrolytes concentrations. Both the initial and delayed hypotensive effects of WSE (50 mg/kg, i.v.) were significantly blocked by pre-treatment with a nonselective nitric oxide (NO) synthase (NOS) inhibitor, NG- nitro-L- arginine methyl ester (L-NAME, 0.325 mg/kg/min for 5 min) or a soluble guanylate cyclase (sGC) inhibitor, 1H- [1,2,4]oxadiazole[4,3- α]quinoxalin-1-one (ODQ, 0.2 mg/kg/min for 5 min). Moreover, whereas the initial depressor effect of WSE was inhibited by pre-treatment with a selective endothelial NOS (eNOS) inhibitor, N5-(1- Iminoethyl)-L-ornithine (L-NIO, 1 mg/kg/min for 5 min), the delayed hypotension was attenuated by a selective inducible NOS (iNOS) inhibitor, S- methylisothiourea (SMT, 0.5 mg/kg/min for 5 min). Administration of WSE also produced an elevation in plasma nitrate/nitrite concentration, as well as an increase in the expression of iNOS protein in the heart and thoracic aorta. These results indicate that WSE from the leaf of M. calabura elicited both a transient and delayed hypotensive effect via the production of NO. Furthermore, activation of NO/sGC/cGMP signaling pathway may mediate the M. calabura-induced hypotension. 8 Today, we observe that some "The Feasibility of Mansanitas (Zizyphus jujuba Linn.) fruit products have increased their Extract as an Alternative source of Sugar" price especially sugar which is one of the basic needs. The researcher made this project for everyone to know that mansanitas is effective as alternative for sugar. Mansanitas as alternative source for sugar is safe, affordable and environmental friendly. The researcher gathers 400 pieces of mansanitas fruit (to produce an estimated amount of 250 mL extract) sieve, container, pan and lather, and stove. The researcher washed the fruit. Then the fruit’s sap was extracted by squeezing using your hands. The extract was filtered using a sieve to remove the bonny and irregularly furrowed stone inside the fruit. The extract was poured in the heated pan and then stirred thoroughly until it became caramelized. Therefore this study is effective as an alternative source for sugar. The researcher recommended to the future investigators to conduct further experimentation about mansanitas. 9 Effects of Muntingia calabura L. on isoproterenol-induced Six groups of Wistar albino rats, myocardial infarction. each comprising six animals, were selected for this study. Group I served as a control, Group II rats were given isoproterenol (20 mg/100g, subcutaneously), and Group III rats were given M. calabura leaf extract (300 mg/kg). Groups IV, V and VI rats were given M. calabura leaf extract (100 mg/kg, 200 mg/kg and 300 mg/kg, respectively) and isoproterenol (20 mg/100g subcutaneously) prior to MI induction. The transaminases (aspartate transaminase and alanine transaminase), lactate dehydrogenase (LDH) and creatine phosphokinase (CK), were estimated in both the serum and heart tissues, and the serum uric acid level was also estimated. 10 The effects of ripe manzanitas fruits (Zizyphus jujuba) on This is an experimental the gastrointestinal motility of white albino mice. randomized controlled study conducted at Cebu Doctors Hospital Research Facility to determine the effect of ripe manzanitas fruit (Zizyphus jujuba) on the gastrointestinal motility of the White Albino Mice Forty healthy white male albino mice of the same age were randomly divided into ten trials with each trial containing four mice to correspond to the four treatment groups (Positive control: Cisapride 0.5 mg in 0.5 cc, Negative control Atropine 0.5 mg in 0.5 cc, Neutral control: Water 0.5 cc, Test substance 100% fruit extract 0.5 cc). All animals were not fed for at least 12 hours except water. Treatment for each trial were given orally using a gavage needle attached to a tuberculin syringe. After 30 minutes all animals were given activated charcoal suspension orally. Then after another 30 minutes, all animals were sacrificed and dissected to exposed the gastrointestinal tract. The length of the small intestine of each animal from the pylorodoudenal junction up to the cecum were measured and recorded as well as the distance travelled by the activated charcoal from the pylorodoudenal junction. From this, the percentage of the distance travelled by the activate charcoal was computed by dividing the distance travelled by the activate charcoal by the length of the small intestine then multiplying the result by 100. The results showed that the distance travelled by the activated charcoal in the test substance group (average percentage of distance: 56.57%) was longer than the negative control group (average percentage: 13.03%) and the neutral control group (average percentage 30.6%). But the distance travelled by the activated charcoal in the positive control group (average percentage 68.61%) was longer than the test substance group. One-way analysis of variance using the f-test at 0.01 level of significance showed that there were significant differences in the average percentage of the distance travelled by the activated charcoal (p-value 0.01) between the treatment groups. Using the Duncan multiple range test at 0.01 level of significance, all means were found to be significantly different from each other This study shows that the ripe Manzanitas fruit (Zizyphus jujuba) has some stimulatory effect on the gastrointestinal tract of the white albino mice 11 Abstract Total antioxidant activity of aratiles and mango extracts on The total antioxidant activities of human low density lipoprotein. aratiles and mango fruit extracts and vitamin C were tested in vitro on human low density lipoprotein (LDL). Samples were incubated with 80 uM copper oxidation catalyst at 37 degrees centigrade for 3 hours. The extent of oxidation was measured by determining the hexanal concentrations and conjugated diene readings. In the hexanal static headspace gas chromatography method, the inhibition of hexanal formation was most effective in the presence of vitamin C, followed by that of the mango extract. Aratiles exhibited prooxidant activity. In the ultraviolet conjugated diene absorbance method, the order of antioxidant activity differed. Aratiles was the best inhibitor of conjugated diene formation, followed by mango, and vitamin C. Concentrations of mango extract higher than 0.50 g/ml may have potential for greater antioxidant activity than what was observed. It is applicable to assess the antioxidant activity of fruit extracts using hexanal gas chromatography. The use of conjugated diene absorbance method needs further modification. (Author) 12 Chemical investigation of the Chemical constituents of Muntingia calabura L. dichloromethane extract of the fruit of Muntingia calaburaafforded squalene (1), triglyceride (2), a mixture of linoleic acid (3a), palmitic acid (3b) and α-linolenic acid (3c), and a mixture of β- sitosterol (4a) and stigmasterol (4b). The structures of 1-4b were identified by comparison of their NMR data with those reported in the literature. Keywords: Muntingia calabura, Muntingiaceae, squalene, triglyceride, β-sitosterol, stigmasterol, linoleic acid, palmitic acid, α-linolenic acid 13 Plant-based antimicrobials Anti-Inflammatory and Anti-Bacterial Efficacy of Aratiles represent a vast untapped source Leaves (Muntingia Calabura Linn) Against Staphylococcus of medicines and further Aureaus exploration of plant antimicrobials needs to occur. Antimicrobials of plant origin have enormous therapeutic potential. . Antimicrobials of plant origin have enormous therapeutic potential. They are effective un treatment of infectious disease while simultaneously mitigating many of the side effects that are often associated with synthetic antimicrobials. Present study was executed to mainly investigate the anti- inflammatory and antibacterial efficacy of Muntingia calabura Linn against Staphylococcus aureaus. This study is done to provide people with an inexpensive, natural and safe anti inflammatory agent. In addition, it may ease the pain of arthritis. The output of this study will help to make anti inflammatory products out of aratilis (Muntingia calabura linn) flowers and leaves. This study might also provide new knowledge about the properties of aratilis (Muntingia calabura Linn). Extracts of aratilis leaves are subjected to several solutions of oil, water and ethanol at 1:1 ratio. Antibacterial efficacy is done using cup cylinder assay by measuring the diameter (mm) of the clear zone around the cup. The results showed that the oil solution showed an average value of 9.03mm, the ethanol solution showed an average value of 18.27 mm and the water solution with an average of 20.10 mm. The negative controls – oil, ethanol and water – exhibited an average of inhibition with values, 8.87 mm, 14.67 mm, and 12.10 mm, respectively. The positive control, Streptomycin sulfate, exhibited a wide range of inhibition with a 24.53 mm clearing. The ointment has no range of inhibition that may due to dilution to the petroleum jelly. 14 Today, we observe that some The Feasibility of Mansanitas (Zizyphus jujuba Linn.) fruit products have increased their Extract as an Alternative source of Sugar price especially sugar which is one of the basic needs. The researcher made this project for everyone to know that mansanitas is effective as alternative for sugar. Mansanitas as alternative source for sugar is safe, affordable and environmental friendly. The researcher gathers 400 pieces of mansanitas fruit (to produce an estimated amount of 250 mL extract) sieve, container, pan and lather, and stove. The researcher washed the fruit. Then the fruit’s sap was extracted by squeezing using your hands. The extract was filtered using a sieve to remove the bonny and irregularly furrowed stone inside the fruit. The extract was poured in the heated pan and then stirred thoroughly until it became caramelized. Therefore this study is effective as an alternative source for sugar. The researcher recommended to the future investigators to conduct further experimentation about mansanitas. 15 Literature has been retrieved Muntingia calabura: A review of its traditional uses, from a number of databases chemical properties, and pharmacological observations (e.g., Pubmed, Science Direct, Springer Link, etc.). General web searches were also carried out using Google and Yahoo search engines by applying some related search terms (e.g., Muntingia calabura, phytochemical, pharmacological, extract, and traditional uses). The articles related to agriculture, ecology, and synthetic work and those using languages other than English or Malay have been excluded. The bibliographies of papers relating to the review subject were also searched for further relevant references. 16 Leaves and stems Bioactive metabolite profiles and weighting 100 g each antimicrobial activity of ethanolic were immersed in 95% ethanol at a ratio of 1:10 extracts from Muntingia calabura L. (w/v) for 72 h. The leaves and stems mixtures were then decanted and filtered. The filtrate was concentrated under reduced pressure using a rotary evaporator (Laborota 4001, Heidolph) with the temperature set at 40 °C. The crude leaf and stem extracts were then air-dried for 14 days. After air-drying, extracts were reconstituted in 95% ethanol, and filtered through a Whatman No. 1 filter paper for further bioassays. 17 This research used 10% MUNTINGIA CALABURA concentration of Muntingia calabura L. leaves, chlorhexidine L LEAVES EXTRACT gluconate 0.12%, and sterile INHIBITS distilled water, as the treatment, positive control and negative GLUCOSYLTRANSFERAS control group, respectively. GTF activity assays through fructose E ACTIVITY OF extensive area analysis by using High Performance Liquid STREPTOCOCCUS Chromatography (HPLC). Fructose extensive area MUTANS determined based on time retention from each groups. Fructose concentrations were expressed in percent (%) then converted into μmol/ml fructose that defined as a unit of GTF activity. The data was analyzed by one-way ANOVA. 18 Muntingia calabura L., Antinociceptive effect of semi-purified Muntingiaceae, is a medicinal petroleum ether partition plant for various pain-related diseases. The aims of the of Muntingia calabura leaves present study were to determine the antinociceptive profile and to elucidate the possible mechanisms of antinociception of petroleum ether partition obtained from crude methanol extract of M. calabura leaves using various animal models. The antinociceptive profile of petroleum ether fraction (given oral; 100, 250 and 500 mg/kg) was established using the in vivo chemicals (acetic acid- induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) models of nociception. The role of glutamate, TRPV1 receptor, bradykinin, protein kinase C, potassium channels, and various opioid and non-opioid receptors in modulating the partition's antinociceptive activity was also determined. The results obtained demonstrated that petroleum ether partition exerted significant (p < 0.05) antinociception in all the chemicals-, thermal-, capsaicin-, glutamate-, bradykinin, and phorbol 12- myristate 13-acetate (PMA)- induced nociception models. The antinociceptive activity was reversed following pretreatment with opioid antagonists (i.e. naloxone, β- funaltrexamine, naltrindole and nor-binaltorphimine), and the non-opioid receptor antagonists (i.e. pindolol (a β- adrenoceptor), haloperidol (a non-selective dopaminergic), atropine (a non-selective cholinergic receptor), caffeine (a non-selective adenosinergic receptor), and yohimbine (an α2-noradrenergic)). In addition, pretreatment with L- arginine (a nitric oxide (NO) donor), NG-nitro-L-arginine methyl esters (L-NAME; an inhibitor of NO synthase (NOS)), methylene blue (MB; an inhibitor of cyclic-guanosine monophosphate (cGMP) pathway), or their combination failed to inhibit petroleum ether partition's antinociception. In conclusion, petroleum ether partition exerts antinociceptive activity at the peripheral and central levels via the modulation of, partly, the opioid (i.e. µ, κ and δ) and several non-opioids (i.e. β-adrenergic, dopaminergic, cholinergic, adenosinergic, and α2- noradrenergic) receptors, glutamatergic, TRPV1 receptors, PKC and K+ channels systems, but not L-arg/NO/cGMP pathway. 19 The present investigation deals TYROSINASE INHIBITION AND ANTI-OXIDANT PROPERTIES with identifying new potential OF MUNTINGIA CALABURA EXTRACTS: IN VITRO STUDIES skin agents from natural origin for tre ating the hyperpigmentation disorders. The medicinal plant, Muntingia calabura was selected as the candidate plant for the present study. 10 % (w/v) extract of various parts (leaf, flower and fruit) of M.calabura ethanol, hy droethanol and petroleum ether) was prepared by decoction method. The antityrosinase activity of the extracts from various parts of the plant was determined by using mushroom tyrosinase as an apt model system and it was found that the hydroethanolic extract of leaves from M. calabura lightening and antioxidant in different solvents (aqueous, possessed the maximum tyrosinase inhibiting potential among the various parts examined. The antioxidant activity of leaf extract of the plant was also ascertained by using 2, 2diphenyl 1picryl hydrazyl (DPPH) scavenging assay and ferric thiocyanate assay. The results showed that DPPH scavenging activity of hydroethanolic extract of leaves was in a dose dependant manner with the IC50 value of 8.5 µ inhibition of lipid peroxidation was almost similar in aqueous and hydroet g. The hanolic leaf extracts of M.calabura. The phenolic content of various solvent extracts of M.calabura leaves was also determined. The hydroethanolic extract of M.calabura leaves exhibited the phenolic content to a greater extent. Our findings revealed that leaves of M.calabura exerted the potent antityrosinase and antioxidant activities. 20 The aim of the present Insecticidal activity of Muntingia study was to evaluate calaburaextracts against larvae and the insecticidal effects of hexane and ethanolic pupae of diamondback, Plutella extracts of the flowers xylostella and fruits of Muntingia calabura against diamondback moth, Plutella xylostella. The leaf disc immersion methodology was carried out to assess the insecticidal effects on the larvae and pupae as well as duration of the larval phase following feeding of the first instar larvae of diamondback on the treated leaf discs for 72 h. All extracts were toxic to larvae and pupae of P. xylostella. The ethanolic extracts of the flowers and fruits of M. calabura were the most toxic against first instar P. xylostella larvae with LC50 values of 0.61 μg mL−1 and 1.63 μg mL−1 respectivel y, followed by the hexane extract of the fruits (LC50 = 5.5 μg mL−1) and flowers (LC50 = 18.9 μg mL−1). All extracts were more toxic to P. xylostella larvae compared with cordycepin, the positive control which produced 100% mortality at 500 μg mL−1 in 72 h. Fruit extracts were more active than the flowers in producing pupal mortality following 72 h of feeding of the first instar larvae on leaf discs treated with the extracts. Both the hexane and the ethanolic extracts of M. calabura fruits and flowers prolonged larval duration by ∼2 days in some cases as compared with the control (7.2 days). These results suggest that M. calabura has potential for development as commercial insecticide for controlling P. xylostella due to its insecticidal effects.