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Basic introduction:
What is light?
The light what we see is just a small region of electromagnetic spectrum,
which human eyes are capable of sense. This region is called as “visible light”.
Why we can see any object and how could we define colors?
When the visible light is reflected from any object and touches the sensors
of our eyes, we can see the objects. In the process of reflection- out of
multiple colors of the radiation, some colors are absorbed and some are
reflected. The color which is reflected is the color what we can see.
Why black colored car is hotter than white colored car during summer?
All the frequencies of white light (all colors) are absorbed by the black
colored car while all the frequencies of white light (all colors) are reflected
by white colored car. So, black colored car is hotter than white colored car
during summer.
It = Io.e-kc It = Io.e-kT
Here, Io = Intensity of initial (original) light
It= Intensity of transmitted light
k = constant
C = concentration
T= Thickness of the solution
Instrumentation:
The source light is passed through filter (to get desired frequency light)
and concentrated using lens. Then this light is targeted on a sample (which is
kept in cuvette). The light transmits through the sample and the
transmitted light is measured by detector. As the intensity of the initial
light is known, we can find the difference between – intensity of initial light
and intensity of transmitted light.
Prism Grating
1. 1.
The light coming from the source must be having proper intensity.
The light source must be having all the frequencies of light, so that the
required frequency can be acquired.
The light source must be stable. It must not change with time.
For different region of light, following lamps (light sources) can be used:
EM Region Light Source Wavelength
1. U.V. / near IR region Hydrogen or Deuterium 10-200 nm
discharge Lamp
2. Visible region Tungsten Lamp 200-1000 nm
3. IR region Nernst glover 1000-10,00,000 nm
Filter and Monochromators:
From this part, the radiation with only specific wavelength or specific
wavelength could be passed. Other radiations are absorbed.
Filters:
Filters allow only a small section of frequency to pass through and all others
are absorbed.
Filters are used mostly in colorimeter. They are made up of glass or gelatin.
By using different filters we can separate different regions of visible light
as follows:
Color of visible region wavelength
1. Violet 380-470 nm
2. Blue 440-490 nm
3. Blue (slightly greenish) 490-500 nm
4. Green 500-560 nm
5. Yellow (slightly greenish) 560-580 nm
6. Yellow 580-600 nm
7. Orange 600-650 nm
8. Red 650-750 nm
Detector: The light which is initiated from the source and pass through
cuvette is measured by the detector. Absorption or transmission of the light
can be measured by detector. Mainly three type of detectors can be used:
1. Photovoltaic Cell (Barrier layer Cell)
2. Photo tubes (Photo emissive tube)
3. Photo multiplier tubes